scholarly journals Encapsulation of Vitamin E in Yogurt-Based Beverage Emulsions: Influence of Bulk Pasteurization and Chilled Storage on Physicochemical Stability and Starter Culture Viability

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1504
Author(s):  
Vassilios Raikos ◽  
Lynn P. Pirie ◽  
Sati Gürel ◽  
Helen E. Hayes
Keyword(s):  
Vitamin E ◽  
Starter Culture ◽  
Chilled Storage ◽  
Culture Viability ◽  
Physicochemical Stability ◽  
Dietary Requirements ◽  
Processing And Storage ◽  
Fat Soluble Vitamins ◽  
And Storage

Yogurt is a nutritious food that is regularly consumed in many countries around the world and is widely appreciated for its organoleptic properties. Despite its contribution to human dietary requirements, yogurt in its traditional recipe is a poor source of fat-soluble vitamins. To respond to consumer demands and further increase the nutritional value of this product, this work aimed to fortify yogurt with vitamin E by using emulsification as the method of encapsulation. The effects of thermal processing and chilled storage on the physicochemical stability of the yogurt-based beverage was investigated. Vitamin E was only minorly affected by bulk pasteurization at 63 °C for 30 min and remained stable during storage at 4 °C for 28 days. Fortified samples showed increased in vitro antioxidant activity compared with non-fortified samples. Lactic acid bacterial counts were above the minimum recommended levels (>106 cfu/g) after processing and storage. In conclusion, this work has demonstrated that emulsification can be an effective strategy for developing yogurt-based products fortified with fat soluble vitamins.

Assuring Growth Inhibition of Listerial Contamination during Processing and Storage of Traditional Greek Graviera Cheese: Compliance with the New European Union Regulatory Criteria for Listeria monocytogenes

2009 ◽  
Vol 72 (11) ◽  
pp. 2264-2271 ◽  
Author(s):  
JOHN SAMELIS ◽  
ELENI GIANNOU ◽  
ALEXANDRA LIANOU
Keyword(s):  
European Union ◽  
Listeria Monocytogenes ◽  
Growth Inhibition ◽  
Starter Culture ◽  
The European Union ◽  
Manufacturing Method ◽  
The Core ◽  
Processing And Storage ◽  
And Storage

The current microbiological regulatory criteria in the European Union specify a maximum Listeria monocytogenes population of 100 CFU/g allowable in ready-to-eat foods provided the product will not exceed this limit throughout its shelf life. The aim of this study was to validate the manufacturing method for traditional Greek Graviera cheese produced from thermized milk. Initial challenge experiments evaluated the fate of inoculated L. monocytogenes (ca. 4 log CFU/ml, three-strain cocktail) in thermized Graviera cheese milk (TGCM; 63°C for 30 s) in the presence and absence of a product-specific starter culture (SC) in vitro. Milk samples were incubated for 6 h at 37°C and then for 66 h at 18°C. Experiments were conducted to evaluate the fate of a cocktail of three nonpathogenic L. monocytogenes and L. innocua indicator strains inoculated (ca. 3 log CFU/g) in Graviera cheeses commercially manufactured from TGCM+SC. Cheeses were brined, ripened at 18°C and 90% relative humidity for 20 days, and stored at 4°C for up to day 60 under vacuum. In TGCM, L. monocytogenes increased by ca. 2 log units, whereas in TGCM+SC L. monocytogenes growth was retarded (P < 0.05) after a ca. 1-log increase within 6 h at 37°C. Populations of Listeria indicator strains did not grow in TGCM+SC cheeses at any stage; they declined 10-fold in fresh cheeses within 5 days and then survived with little death thereafter. Thus, growth inhibition but not inactivation of potent natural Listeria contaminants at levels below 100 CFU/g occurs in the core of traditional Greek Graviera cheese during fermentation, ripening, and storage.

scholarly journals Impact of storage conditions and premix type on fat-soluble vitamin stability1

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Marut Saensukjaroenphon ◽  
Caitlin E Evans ◽  
Chad B Paulk ◽  
Jordan T Gebhardt ◽  
Jason C Woodworth ◽  
...  
Keyword(s):  
Vitamin E ◽  
Vitamin A ◽  
Chemical Treatment ◽  
Storage Time ◽  
Storage Condition ◽  
The United States ◽  
Heat Pulse ◽  
Pulse Treatment ◽  
Fat Soluble Vitamins ◽  
And Storage

Abstract Feed ingredients and additives could be a potential medium for foreign animal disease entry into the United States. The feed industry has taken active steps to reduce the risk of pathogen entry through ingredients. Medium chain fatty acid (MCFA) and heat pulse treatment could be an opportunity to prevent pathogen contamination. The objective of experiment 1 was to determine the impact of 0, 30, 60, or 90 d storage time on fat-soluble vitamin stability when vitamin premix (VP) and vitamin trace mineral premix (VTM) were blended with 1% inclusion of MCFA (1:1:1 blend of C6:C8:C10) or mineral oil (MO) with different environmental conditions. Samples stored at room temperature (RT) (~22 °C) or in an environmentally controlled chamber set at 40 °C and 75% humidity, high-temperature high humidity (HTHH). The sample bags were pulled out at days 0, 30, 60 and 90 for RT condition and HTHH condition. The objective of experiment 2 was to determine the effect of heat pulse treatment and MCFA addition on fat-soluble vitamin stability with two premix types. A sample from each treatment was heated at 60 °C and 20% humidity. For experiment 1, the following effects were significant for vitamin A: premix type × storage condition (P = 0.031) and storage time × storage condition (P = 0.002) interactions; for vitamin D3: main effect of storage condition (P < 0.001) and storage time (P = 0.002); and for vitamin E: storage time × storage condition interaction (P < 0.001). For experiment 2, oil type did not affect the stability of fat-soluble vitamins (P > 0.732) except for vitamin A (P = 0.030). There were no differences for fat-soluble vitamin stability between VP and VTM (P > 0.074) except for vitamin E (P = 0.016). Therefore, the fat-soluble vitamins were stable when mixed with both vitamin and VTM and stored at 22 °C with 28.4% relative humidity (RH). When premixes were stored at 39.5 °C with 78.8%RH, the vitamin A and D3 were stable up to 30 d while the vitamin E was stable up to 60 d. In addition, MCFA did not influence fat-soluble vitamin degradation during storage up to 90 d and in the heat pulse process. The vitamin stability was decreased by 5% to 10% after the premixes was heated at 60 °C for approximately nine and a half hours. If both chemical treatment (MCFA) and heat pulse treatment have similar efficiency at neutralizing or reducing the target pathogen, the process of chemical treatment could become a more practical practice.

Processing and storage of lye-treated carrots fermented by a mixed starter culture

1997 ◽  
Vol 35 (1) ◽  
pp. 83-90 ◽  
Author(s):  
Alfredo Montaño ◽  
Antonio H. Sánchez ◽  
Luis Rejano ◽  
A. de Castro
Keyword(s):  
Starter Culture ◽  
Processing And Storage ◽  
And Storage

Influence of different processing and storage conditions on in vitro bioaccessibility of polyphenols in black carrot jams and marmalades

2015 ◽  
Vol 186 ◽  
pp. 74-82 ◽  
Author(s):  
Senem Kamiloglu ◽  
Ayca Ayfer Pasli ◽  
Beraat Ozcelik ◽  
John Van Camp ◽  
Esra Capanoglu
Keyword(s):  
Storage Conditions ◽  
Black Carrot ◽  
In Vitro Bioaccessibility ◽  
Processing And Storage ◽  
And Storage

scholarly journals Antioxidant capacity and lipid stability egg during processing and storage.

2014 ◽  
Vol 4 (1) ◽  
pp. 19
Author(s):  
Michelle Garcêz Carvalho ◽  
Alfredo Tenuta Filho
Keyword(s):  
Oxidative Stability ◽  
Antioxidant Capacity ◽  
Lipid Fraction ◽  
Thiobarbituric Acid ◽  
Storage Conditions ◽  
Limit Of Quantification ◽  
Total Antioxidant ◽  
Processing And Storage ◽  
And Storage

<p>Different processing conditions, packaging and storage can affect the natural antioxidant protection of the egg. Thus, the objectives were: (a) To standardize a methodology able to evaluate in vitro antioxidant capacity of fresh and processed egg, (b) investigate the effect of pasteurization and spray on the oxidative stability of the lipid fraction of egg and (c) evaluate the antioxidant capacity and oxidative stability of fatty acids from whole egg pasteurized atomized, bottle of high density polyethylene, opaque, under nitrogen, at 5&plusmn;2&ordm;C for up to 90 days. The phosphomolybdenum method to measure the total antioxidant capacity of lipid fraction (CATL) Egg presented analytical adequacy, with a limit of quantification of 0.017 mg &alpha;-tocoferol/mL. The CATL decreased with the progress of processing and the reverse was observed for the lipids, 7-CETO (7-ketocholesterol) and TBARS (thiobarbituric acid reactive substances). The pasteurized egg atomized (OIPA) kept under ideal storage conditions considered stable in relation to hydration, CATL and TBARS. It is concluded that the method of phosphomolybdenum suitability presented analytical sufficient. Pasteurization did not affect any of the parameters evaluated, but the atomization caused significant decrease CATL, and elevated lipids, TBARS and 7-CETO. Was maintained hydration and stability of the lipid OIPA stored for 90 days at 5&deg;C, indicating that the conditions for packaging and storage were effective.&nbsp;</p><p>&nbsp;</p><p>DOI:&nbsp;<a href="http://dx.doi.org/10.14685/rebrapa.v4i1.102">http://dx.doi.org/<span>10.14685/rebrapa.v4i1.</span><span>102</span></a></p><p><span><br /></span></p>

scholarly journals Inhibition Mechanism of L-Cysteine on Maillard Reaction by Trapping 5-Hydroxymethylfurfural

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1391
Author(s):  
Shiqiang Yang ◽  
Zhongfei Zhang ◽  
Jiaoyong Li ◽  
Yuge Niu ◽  
Liangli Lucy Yu
Keyword(s):  
Maillard Reaction ◽  
Antioxidant Activities ◽  
Nutrient Utilization ◽  
In Vitro Assays ◽  
Raw 264.7 Cells ◽  
Inhibition Mechanism ◽  
Safety And Health ◽  
Processing And Storage ◽  
And Storage

The Maillard reaction (MR) can affect the color, flavor, organoleptic properties, and nutritional value of food. Sometimes, MR is undesirable due to lowering the nutrient utilization, producing harmful neo-formed compounds, etc. In this case, it is necessary to control MR. Some chemical substances, such as phenolic acid, vitamins, aminoguanidine, and thiols extracted from garlic or onion, can effectively prevent MR. In this study, L-cysteine (L-cys) was found to inhibit MR after screening 10 sulfhydryl compounds by comparing their ability to mitigate browning. The inhibition mechanism was speculated to be related to the removal of 5-hydroxymethylfurfural (HMF), a key mid-product of MR. The reaction product of HMF and L-cys was identified and named as 1-dicysteinethioacetal–5-hydroxymethylfurfural (DCH) according to the mass spectrum and nuclear magnetic resonance spectrum of the main product. Furthermore, DCH was detected in the glutamic–fructose mixture after L-cys was added. In addition, the production of DCH also increased with the addition of L-cys. It also was worth noting that DCH showed no cell toxicity to RAW 264.7 cells. Moreover, the in vitro assays indicated that DCH had anti-inflammatory and antioxidant activities. In conclusion, L-cys inhibits MR by converting HMF into another adduct DCH with higher safety and health benefits. L-cys has the potential to be applied as an inhibitor to prevent MR during food processing and storage.

scholarly journals Short Communication: Quantification of the Effect of Mycotoxin Binders on the Bioavailability of Fat-Soluble Vitamins In Vitro

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2251
Author(s):  
Abdelhacib Kihal ◽  
María Ercilda Rodríguez-Prado ◽  
Carles Cristofol ◽  
Sergio Calsamiglia
Keyword(s):  
Vitamin D ◽  
Vitamin E ◽  
Yeast Cell ◽  
Active Carbon ◽  
Cell Walls ◽  
Short Communication ◽  
In Vitro System ◽  
Main Factors ◽  
Fat Soluble Vitamins

The aim of this study was to determine the capacity of six mycotoxin binders (MTBs) to adsorb vitamins A, D and E in an in vitro system that simulates gastric and intestinal digestion. Experiment 1 evaluated the recovery rate of vitamins A, D and E in the incubation conditions. In Experiment 2, the main factors were the MTB (bentonite, clinoptilolite, sepiolite, montmorillonite, active carbon and yeast cell walls), vitamins (A, D and E) and incubation type (vitamins incubated separately or together). The recovery was high for vitamin D (83%) and E (93%), but low for vitamin A (23%), for which no further analyses were conducted. When incubated separately, vitamin D was only adsorbed by yeast cell wall (20.2%). Vitamin E adsorption was highest with bentonite (54.5%) and montmorillonite (46.3%) and lowest with sepiolite (16.6%) and active carbon (18.5%). When incubated together, vitamin D was not adsorbed by any MTB. Vitamin E adsorption was highest in bentonite (61.8%) and montmorillonite (50.7%) and lowest in sepiolite (15.4%). Results indicate that the bioavailability of vitamin E, but not that of vitamin D, may be reduced in the presence of MTBs.

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