scholarly journals Collagenated Porcine Heterologous Bone Grafts: Histomorphometric Evaluation of Bone Formation Using Different Physical Forms in a Rabbit Cancellous Bone Model

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1339
Author(s):  
Rui I. Falacho ◽  
Paulo J. Palma ◽  
Joana A. Marques ◽  
Maria H. Figueiredo ◽  
Francisco Caramelo ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Cancellous Bone ◽  
Critical Size ◽  
Femoral Bone ◽  
New Bone Formation ◽  
Qualitative And Quantitative ◽  
Bone Particle ◽  
Post Surgery ◽  
New Zealand White Rabbits

Collagenated porcine-derived bone graft materials exhibit osteoconductive properties and the development of different formulations intends to enhance bone regeneration. This study aims to evaluate bone healing in a rabbit cancellous bone defect in response to grafting with different physicochemical forms of heterologous porcine bone. Twenty-six adult male New Zealand White rabbits received two critical size femoral bone defects per animal (n = 52), each randomly assigned to one of the five tested materials (Apatos, Gen-Os, mp3, Putty, and Gel 40). Animals were sacrificed at 15- and 30-days post-surgery. Qualitative and quantitative (new bone, particle and connective tissue percentages) histological analyses were performed. Histomorphometry showed statistically significant differences in all evaluated parameters between mp3 and both Putty and Gel 40 groups, regardless of the timepoint (p < 0.05). Moreover, statistical differences were observed between Apatos and both Putty (p = 0.014) and Gel 40 (p = 0.007) groups, at 30 days, in regard to particle percentage. Within each group, regarding new bone formation, mp3 showed significant differences (p = 0.028) between 15 (40.93 ± 3.49%) and 30 (52.49 ± 11.04%) days. Additionally, intragroup analysis concerning the percentage of particles revealed a significant reduction in particle occupied area from 15 to 30 days in mp3 and Gen-Os groups (p = 0.009). All mp3, Gen-Os and Apatos exhibited promising results in terms of new bone formation, thus presenting suitable alternatives to be used in bone regeneration.

scholarly journals BAG S53P4 putty as bone graft substitute – a rabbit model

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Ilkka Saarenpää ◽  
Patricia Stoor ◽  
Janek Frantzén
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Proximal Tibia ◽  
Rabbit Model ◽  
Bone Graft Substitute ◽  
Control Group ◽  
The Novel ◽  
Bone Augmentation ◽  
New Bone Formation ◽  
New Zealand White Rabbits

AbstractBioactive glass (BAG) S53P4 granules represent a bone augmentation biomaterial for the surgical treatment of bony defects, even in challenging conditions such as osteomyelitis. The aim of this eight-week rabbit implantation study was to evaluate the biocompatibility and bone regeneration performance of a BAG S53P4 putty formulation following its implantation into the proximal tibia bone of twenty-eight New Zealand white rabbits. BAG S53P4 putty was compared to BAG S53P4 granules (0.5-0.8 mm) to evaluate whether the synthetic putty binder influences the bone regeneration of the osteostimulative granules. The putty formulation facilitates clinical use because of its mouldability, injectability and ease of mixing with autograft. Implantation of putty and granules into proximal tibia defects resulted in good osseointegration of the two groups. Both biomaterials were biocompatible, showed high new bone formation, high vascularization and periosteal growth. No signs of disturbed bone formation were observed due to the PEG-glycerol binder in the BAG S53P4 putty. Instead, intramedullary ossification and stromal cell reaction were more advanced in the putty group compared to the control group (p = 0.001 and p < 0.001). In conclusion, the novel mouldable BAG S53P4 putty showed reliable bone regeneration in bony defects without adverse tissue or cell reactions.

scholarly journals Lack of repair of rat skull critical size defect treated with bovine morphometric protein bound to microgranular bioabsorbable hydroxyapatite

2004 ◽  
Vol 15 (3) ◽  
pp. 175-180 ◽  
Author(s):  
Gabriel Ramalho Ferreira ◽  
Tania Mary Cestari ◽  
José Mauro Granjeiro ◽  
Rumio Taga
Keyword(s):  
Foreign Body ◽  
Bone Formation ◽  
Bone Morphogenetic Proteins ◽  
Bone Repair ◽  
Critical Size ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Body Type ◽  
Granulomatous Reaction ◽  
Post Surgery

The ability of a pool of bovine bone morphogenetic proteins bound to synthetic microgranular hydroxyapatite (BMPb-HA) to stimulate bone repair was determined in rat critical size defects. An 8-mm diameter defect was created in the calvaria of 25 rats. In 15 rats, the defects were filled with BMPb-HA homogenized with blood (experimental group), and in 10 rats the defects were filled only with blood clots (control). The calvariae of experimental rats were collected 1, 3 and 6 months after surgery and of the control rats at the end of surgery and 6 months thereafter. The morphometric results obtained in the radiographs showed an absence of new bone formation at 1 and 3 months post-surgery and, histologically, the defects were filled with fibrous connective tissue and numerous foci of a foreign body-type granulomatous reaction around hydroxyapatite agglomerates. At the end of 6 months, the number and size of the granulomatous foci decreased and the area of the defects was reduced by 22% compared to the 0-hour control due to the formation of new bone at their borders, although the mean area was similar to the 6-month control. We conclude that the use of BMPb-HA in the treatment of critical size bone defects of the rat skull leads to the formation of a foreign body-type granulomatous reaction that markedly inhibits new bone formation, suggesting that synthetic microgranular hydroxyapatite does not represent a good carrier for BMP-induced bone formation.

scholarly journals Digital Customized Titanium Mesh for Bone Regeneration of Vertical, Horizontal and Combined Defects: A Case Series

Medicina ◽  
2021 ◽  
Vol 57 (1) ◽  
pp. 60
Author(s):  
Daniele De Santis ◽  
Federico Gelpi ◽  
Giuseppe Verlato ◽  
Umberto Luciano ◽  
Lorena Torroni ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Case Series ◽  
Guided Bone Regeneration ◽  
Point Of View ◽  
Significant Gain ◽  
Titanium Mesh ◽  
New Bone Formation ◽  
Correct Method ◽  
Combined Defects

Background and Objective: Guided bone regeneration allows new bone formation in anatomical sites showing defects preventing implant rehabilitation. Material and Methods: The present case series reported the outcomes of five patients treated with customized titanium meshes manufactured with a digital workflow for achieving bone regeneration at future implant sites. A significant gain in both width and thickness was achieved for all patients. Results: From a radiographic point of view (CBTC), satisfactory results were reached both in horizontal and vertical defects. An average horizontal gain of 3.6 ± 0.8 mm and a vertical gain of 5.2 ± 1.1 mm. Conclusions: The findings from this study suggest that customized titanium meshes represent a valid method to pursue guided bone regeneration in horizontal, vertical or combined defects. Particular attention must be paid by the surgeon in the packaging of the flap according to a correct method called the “poncho” technique in order to reduce the most frequent complication that is the exposure of the mesh even if a partial exposure of one mesh does not compromise the final outcome of both the reconstruction and the healing of the implants.

scholarly journals The use of solvent-preserved human and bovine cancellous bone blocks for lateral defect augmentation - an experimental controlled study in vivo

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Lara Schorn ◽  
Tim Fienitz ◽  
Kathrin Berndsen ◽  
Norbert R. Kübler ◽  
Henrik Holtmann ◽  
...  
Keyword(s):  
Bone Formation ◽  
Cancellous Bone ◽  
Bone Substitute ◽  
Bone Matrix ◽  
Human Bone ◽  
Controlled Study ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Bone Substitute Material ◽  
Substitute Material

Abstract Background The aim of this study was to compare new bone formation, resorbed bone matrix, and fibrous enclosed residual bone substitute material in laterally augmented alveolar bone defects using allogeneic, pre-treated and cleaned human bone blocks (tested in dogs, therefore considered to be xenogeneic), and pre-treated and cleaned bovine cancellous bone blocks, both with and without a collagen membrane in order to evaluate their augmentative potential. Methods Thirty-two critical size horizontal defects were prepared in the mandible of 4 adult foxhound dogs (8 per dog, 4 on each side). After 3 months of healing, the defects were laterally augmented in a split-mouth-design with either human (HXB) or bovine solvent-preserved bone blocks (BXB). Afterwards, defects were randomly covered with a bovine collagenous membrane (HXB + M, BXB + M). After a healing interval of 6 months, percentages of new bone formation, resorbed bone matrix, and fibrous enclosed residual bone substitute material were compared. Results Results showed little new bone formation of up to 3.7 % in human bone blocks (HXB 3.7 % ± 10.2, HXB + M 0.3 %± 0.4, BXB, 0.1 % ± 0.8, BXB + M 2.6 % ± 3.2, p = > 0.05). Percentages of fibrous encapsulation were higher in human bone blocks than in bovine bone blocks (HXB 71.2 % ± 8.6, HXB + M 73.71 % ± 10.6, BXB, 60.5 % ± 27.4, BXB + M 52.5 % ± 28.4, p = > 0.05). Resorption rates differed from 44.8 % in bovine bone blocks covered with a membrane to 17.4 % in human bone blocks (HXB 17.4 % ± 7.4, HXB + M 25.9 % ± 10.7, BXB, 38.4 % ± 27.2, BXB + M 44.8 % ± 29.6, p = > 0.05). The use of additional membranes did not significantly affect results. Conclusions Within its limitations, results of this study suggest that solvent-preserved xenogenic human and bovine bone blocks are not suitable for lateral bone augmentation in dogs. Furthermore, defect coverage with a membrane does not positively affect the outcome.

scholarly journals BMSCs Seeding in Different Scaffold Incorporation with Hyperbaric Oxygen Treat Seawater Immersed Bony Defect

2021 ◽  
Author(s):  
gan zhang ◽  
Xiaosong Chen ◽  
Xunsheng Cheng ◽  
Xiuwu Ma ◽  
Congcong Chen
Keyword(s):  
Bone Formation ◽  
Bone Defect ◽  
Medullary Canal ◽  
Bony Defect ◽  
New Bone Formation ◽  
X Ray ◽  
New Zealand White Rabbits ◽  
Group A ◽  
Group B ◽  
Group D

Abstract Introduction: The experiment was undertaken to estimate the effect of BMSCs seeding in different scaffold incorporation with HBO on the repair of seawater immersed bone defect. And future compared n-HA/PLGA with β-TCP/PLGA as scaffold in treatment effect of seawater immersed bone defect.Methods: 60 New Zealand White rabbits with standard seawater defect in radius were randomly divided to group A (implant with nothing), group B (implanted with atuogenous bone), group C (implanted with n-HA/PLGA/BMSCs, and Group D ( implanted with β-TCP/PLGA/BMSCs). After implant, each rabbit receive HBO treatment at 2.4 ATA 100% oxygen for 120 minutes per day for 2 weeeks. Radiograph, histological and biomechanical examination were used to analyze osteogenesis.Result: X-ray analysis show that n-HA/PLGA/BMSCs and β-TCP/PLGA/BMSCs could accelerate the new bone formation, and the new bone formation in group C was lager than in group D or group A, and close to group B (P<0.05). After 12 weeks, in group A, defect without scaffold show a loose connect tissue filled in the areas. The medullary canal in group B was recanalizated. Defect in group C and D show a larger number of wove bone formation. The new wove bone formation in defect areas in group C was lager than D. The mechanical examination revealed ultimate strength at 12 weeks were group D>group C>group B>group A(P<0.05).Conclusion: Scaffold of n-HA/PLGA and β-TCP/PLGA incorporation with HBO and BMSCs were effective to treat seawater immersed bone defect, and n-HA/PLGA was more excellent than β-TCP/PLGA.

Enhanced Effects of New Bone Formation by an Electrically Polarized Hydroxyapatite Microgranule/Platelet-Rich Plasma Composite Gel

2012 ◽  
Vol 529-530 ◽  
pp. 82-87 ◽  
Author(s):  
Seiko Ohba ◽  
Wei Wang ◽  
Soichiro Itoh ◽  
Akiko Nagai ◽  
Kimihiro Yamashita
Keyword(s):  
Bone Formation ◽  
Histological Examination ◽  
Platelet Rich Plasma ◽  
New Bone Formation ◽  
Electrical Polarization ◽  
Osteogenic Cells ◽  
Post Surgery ◽  
Composite Gel

The effects of electrically polarized HA microgranule/PRP compositeon new bone formation were examined. The composite gel was implanted into bone holes in rabbits. Histological examination was performed 3 and 6 weeks post-surgery. It was hypothesized that PRP alone could not induce new bone formation until 6 weeks after implantation. HA microgranules with or without electrical polarization/PRP composite, especially the former, activated osteogenic cells, resulting in enhanced bone formation. It was confirmed that electrical polarization treatment of HA microgranules can accelerate new bone formation and this effect is enhanced by forming a complex within PRP.

scholarly journals Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model

Materials ◽  
2019 ◽  
Vol 12 (10) ◽  
pp. 1613
Author(s):  
Eunhye Jang ◽  
Ja-Youn Lee ◽  
Eun-Young Lee ◽  
Hyun Seok
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Human Bone ◽  
Control Group ◽  
New Bone Formation ◽  
Morphogenetic Protein ◽  
Regeneration Effect ◽  
Recombinant Human Bone

The aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP) (5 µg), and ABB/high BMP (50 µg) were grafted as subperiosteal bone grafts. The new bone formation parameters of bone volume (BV), bone mineral density (BMD), trabecular thickness (TbTh), and trabecular spacing (TbSp) were evaluated by microcomputed tomography (µ-CT), and a histomorphometric analysis was performed to evaluate the new bone formation area. The expression of osteogenic markers, such as bone sialoprotein (BSP) and osteocalcin, were evaluated by immunohistochemistry (IHC). The ABB/high BMP group showed significantly higher BV than the ABB/low BMP (p = 0.004) and control groups (p = 0.000) and higher TbTh than the control group (p = 0.000). The ABB/low BMP group showed significantly higher BV, BMD, and TbTh than the control group (p = 0.002, 0.042, and 0.000, respectively). The histomorphometry showed significantly higher bone formation in the ABB/low and high BMP groups than in the control group (p = 0.000). IHC showed a high expression of BSP and osteocalcin in the ABB/low and high BMP groups. Subperiosteal bone grafts with ABB and rhBMP-2 have not been studied. In our study, we confirmed that rhBMP-2 contributes to new bone formation in a subperiosteal bone graft with ABB.

scholarly journals Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs

2020 ◽  
Vol 8 (1) ◽  
pp. 7 ◽  
Author(s):  
Savvas Titsinides ◽  
Theodore Karatzas ◽  
Despoina Perrea ◽  
Efstathios Eleftheriadis ◽  
Leonidas Podaropoulos ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Repair ◽  
Healing Process ◽  
Bone Substitutes ◽  
Bone Defects ◽  
New Bone Formation ◽  
Post Surgery ◽  
Beta Tricalcium Phosphate ◽  
Close Proximity ◽  
Tissue Specimens

Regeneration of large jaw bone defects still remains a clinical challenge. To avoid incomplete bone repair, bone grafts have been advocated to support the healing process. This study comparatively evaluated new bone formation among a synthetic graft substitute, a human bone derivative, and a bovine xenograft. Materials were placed in 3 out of the 4 bone cavities, while 1 deficit was left empty, serving as a control, in mono-cortical defects, surgically prepared in the porcine calvaria bone. Animals were randomized in 2 groups and euthanized at 8 and 12 weeks. Harvested tissue specimens were qualitatively evaluated by histology. New bone formation was quantitatively measured by histomorphometry. Maximum new bone formation was noticed in defects grafted with beta-tricalcium phosphate b-TCP compared to the other bone substitutes, at 8 and 12 weeks post-surgery. Bovine and human allograft induced less new bone formation compared to empty bone cavity. Histologic analysis revealed that b-TCP was absorbed and substituted significantly, while bovine and human allograft was maintained almost intact in close proximity with new bone. Based on our findings, higher new bone formation was detected in defects filled with b-TCP when compared to bovine and human graft substitutes.

Age-related new bone formation following the use of cancellous bone-block allografts for reconstruction of atrophic alveolar ridges

2017 ◽  
Vol 20 (1) ◽  
pp. 4-8 ◽  
Author(s):  
Joseph Nissan ◽  
Roni Kolerman ◽  
Liat Chaushu ◽  
Marilena Vered ◽  
Sarit Naishlos ◽  
...  
Keyword(s):  
Bone Formation ◽  
Cancellous Bone ◽  
Bone Block ◽  
New Bone Formation ◽  
Age Related
Sign in / Sign up

Export Citation Format

Share Document