scholarly journals Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of Tagetes erecta L. Flowers

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1201
Author(s):  
Ana Flavia Burlec ◽  
Łukasz Pecio ◽  
Solomiia Kozachok ◽  
Cornelia Mircea ◽  
Andreia Corciovă ◽  
...  
Keyword(s):  
Biological Activities ◽  
In Vitro Cytotoxicity ◽  
In Vitro Assays ◽  
Tagetes Erecta ◽  
Phytochemical Analysis ◽  
Total Extract ◽  
Tagetes Erecta L ◽  
Cytotoxicity Assessment

Tagetes erecta L. is a popular ornamental plant of the Asteraceae family, which is widely cultivated not only for its decorative use, but also for the extraction of lutein. Besides carotenoid representatives, which have been extensively studied, other important classes of secondary metabolites present in the plant, such as polyphenols, could exhibit important biological activities. The phytochemical analysis of a methanolic extract obtained from T. erecta inflorescences was achieved using liquid chromatography–mass spectrometry (LC-MS) techniques. The extract was further subjected to a multistep purification process, which allowed the separation of different fractions. The total extract and its fractions contain several polyphenolic compounds, such as hydroxybenzoic and hydroxycinnamic acid derivatives, flavonols (especially quercetagetin glycosides), and several aglycons (e.g., quercetin, patuletin). One of the fractions, containing mostly quercetagitrin, was subjected to two different antioxidant assays (metal chelating activity and lipoxygenase inhibition) and to in vitro cytotoxicity assessment. Generally, the biological assays showed promising results for the investigated fraction compared to the initial extract. Given the encouraging outcome of the in vitro assays, further purification and structural analysis of compounds from T. erecta extracts, as well as further in vivo investigations are justified.

Tamarix articulata (T. articulata) - An Important Halophytic Medicinal Plant with Potential Pharmacological Properties

2019 ◽  
Vol 20 (4) ◽  
pp. 285-292 ◽  
Author(s):  
Abdullah M. Alnuqaydan ◽  
Bilal Rah
Keyword(s):  
Medicinal Plant ◽  
Biological Activities ◽  
Wide Spectrum ◽  
Biological Properties ◽  
In Vivo Model ◽  
Drug Candidate ◽  
Phytochemical Analysis ◽  
Pharmacological Properties

Background:Tamarix Articulata (T. articulata), commonly known as Tamarisk or Athal in Arabic region, belongs to the Tamaricaece species. It is an important halophytic medicinal plant and a good source of polyphenolic phytochemical(s). In traditional medicines, T. articulata extract is commonly used, either singly or in combination with other plant extracts against different ailments since ancient times.Methods:Electronic database survey via Pubmed, Google Scholar, Researchgate, Scopus and Science Direct were used to review the scientific inputs until October 2018, by searching appropriate keywords. Literature related to pharmacological activities of T. articulata, Tamarix species, phytochemical analysis of T. articulata, biological activities of T. articulata extracts. All of these terms were used to search the scientific literature associated with T. articulata; the dosage of extract, route of administration, extract type, and in-vitro and in-vivo model.Results:Numerous reports revealed that T. articulata contains a wide spectrum of phytochemical(s), which enables it to have a wide window of biological properties. Owing to the presence of high content of phytochemical compounds like polyphenolics and flavonoids, T. articulata is a potential source of antioxidant, anti-inflammatory and antiproliferative properties. In view of these pharmacological properties, T. articulata could be a potential drug candidate to treat various clinical conditions including cancer in the near future.Conclusion:In this review, the spectrum of phytochemical(s) has been summarized for their pharmacological properties and the mechanisms of action, and the possible potential therapeutic applications of this plant against various diseases discussed.

scholarly journals Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee Melipona fasciculata

2019 ◽  
Vol 20 (18) ◽  
pp. 4512 ◽  
Author(s):  
Alberto Jorge Oliveira Lopes ◽  
Cleydlenne Costa Vasconcelos ◽  
Francisco Assis Nascimento Pereira ◽  
Rosa Helena Moraes Silva ◽  
Pedro Felipe dos Santos Queiroz ◽  
...  
Keyword(s):  
Native Species ◽  
Biological Activities ◽  
Stingless Bee ◽  
In Vitro Assays ◽  
Pollen Extract ◽  
Biological Potential ◽  
Anti Inflammatory ◽  
Analgesic Activities

The stingless bee, Melipona fasciculata Smith (Apidae, Meliponini), is a native species from Brazil. Their products have high biotechnological potential, however there are no studies about the biological activities of pollen collected by M. fasciculata. In this context, the present study investigated the chemical composition, anti-oxidant, anti-inflammatory, and analgesic activities of hydroethanolic pollen extracts collected by M. fasciculata in three cities in Maranhão State, Brazil. We verified the antioxidant activity of the extracts and inhibitory activity against the cyclooxygenase enzyme using in vitro assays and in allowed to select the extract with higher efficiency to be used on in vivo assays. In these trials, the selected extract showed high anti-inflammatory activity as well as nociceptive effects at central and peripheral level, suggesting that this extract acts on inhibition of histamine release and decreased synthesis of prostaglandins and the in-silico study suggested that polyphenols and acids fatty acids in the extract may be associated with these activities. The results of the present study report the high biological potential of pollen extract and we conclude that the pollen collected by M. fasciculata can be considered as the object of research for new pharmacological alternatives.

scholarly journals Biological Activities of Novel Gyrase Inhibitors of the Aminocoumarin Class

2008 ◽  
Vol 52 (6) ◽  
pp. 1982-1990 ◽  
Author(s):  
Christine Anderle ◽  
Martin Stieger ◽  
Matthew Burrell ◽  
Stefan Reinelt ◽  
Anthony Maxwell ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Rank Order ◽  
Biological Activities ◽  
Dna Supercoiling ◽  
Binding Energies ◽  
In Vitro Assays ◽  
Gene Assay ◽  
Gyrase Inhibitors

ABSTRACT Thirty-one aminocoumarin antibiotics derived from mutasynthesis experiments were investigated for their biological activities. Their inhibitory activities toward Escherichia coli DNA gyrase were determined in two different in vitro assays: an ATPase assay and a DNA supercoiling assay. The assays gave a similar rank order of the activities of the compounds tested, although the absolute 50% inhibitory concentrations (IC50s) obtained in each assay were different. To confirm that the compounds also acted as gyrase inhibitors in vivo, reporter gene assays were carried out with E. coli by using gyrA and sulA promoter fusions with the luxCDABE operon. A strong induction of both promoters was observed for those compounds that showed gyrase inhibitory activity in the biochemical assays. Compounds carrying analogs of the prenylated benzoyl moiety (ring A) of clorobiocin that were structurally very different showed high levels of activity both in the biochemical assay and in the reporter gene assay, indicating that the structure of this moiety can be varied considerably without a loss of affinity for bacterial gyrase. The experimentally determined IC50s were compared to the binding energies calculated in silico, which indicated that a shift of the pyrrole carboxylic acid moiety from the O-3″ to the O-2″ position of the deoxysugar moiety has a significant impact on the binding mode of the compounds. The aminocoumarin compounds were also investigated for their MICs against different bacterial pathogens. Several compounds showed high levels of activity against staphylococci, including a methicillin-resistant Staphylococcus aureus strain. However, they showed only poor activities against gram-negative strains.

EVALUATION OF THE BOVINE TESTICULAR RADIORECEPTOR ASSAY FOR PITUITARY FOLLICLE-STIMULATING HORMONE

1979 ◽  
Vol 82 (2) ◽  
pp. 253-262 ◽  
Author(s):  
M. R. SAIRAM
Keyword(s):  
Biological Activities ◽  
Follicle Stimulating Hormone ◽  
Radioreceptor Assay ◽  
In Vitro Assays ◽  
Receptor Interactions ◽  
Testicular Receptor ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Stimulating Hormone

SUMMARY A modified bovine testicular receptor was used to evaluate highly purified follicle-stimulating hormone (FSH) from a number of species. The particulate receptor obtained from adult bovine testes could be stored frozen or lyophilized for long periods without appreciable decrease in the binding of the ligand facility or in loss of specificity. The bovine testis receptor binds twice as much 125I-labelled ovine FSH as 125I-labelled human hormone. When FSH from different species was compared against NIH-FSH-S10, using various FSH ligands, the ovine hormone was clearly the most active, although many had comparable in-vivo biological potencies. The results suggest that there is probably some species specificity in the hormone–receptor interactions. As the ovine hormone is structurally closer to the bovine, from which the receptor was derived, it appears to have the highest activity in vitro. Marked differences in the biological activities of the different preparations between the human chorionic gonadotrophin-augmentation test and the in-vitro assays have been observed. In the in-vitro assays, all preparations, with the exception of the porcine hormone preparation, were less active and the ratio of bioassay/radioreceptor assay varied widely. In the radioreceptor assays, all FSH preparations except pregnant mare serum gonadotrophin (PMSG) showed parallel inhibition curves. The three different PMSG preparations examined gave inhibition lines that were parallel to each other.

scholarly journals Physicochemical Characterization and Biological Activities of Black and White Garlic: In Vivo and In Vitro Assays

Foods ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 220 ◽  
Author(s):  
María Ángeles Toledano Medina ◽  
Tania Merinas-Amo ◽  
Zahira Fernández-Bedmar ◽  
Rafael Font ◽  
Mercedes del Río-Celestino ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Genetic Model ◽  
Biological Activities ◽  
Leukemia Cell ◽  
Physicochemical Characterization ◽  
Biological Data ◽  
In Vitro Assays ◽  
Age Related

White and three types of black garlic (13, 32, and 45 days of aging, named 0C1, 1C2, and 2C1, respectively) were selected to study possible differences in their nutraceutic potential. For this purpose, garlic were physicochemically characterized (Brix, pH, aW, L, polyphenol, and antioxidant capacity), and both in vivo and in vitro assays were carried out. Black garlic samples showed higher polyphenol content and antioxidant capacity than the white ones. The biological assays showed that none of the samples (neither raw nor black garlic) produced toxic effects in the Drosophila melanogaster animal genetic model, nor exerted protective effects against H2O2, with the exception of the 0C1 black garlic. Moreover, only white garlic was genotoxic at the highest concentration. On the other hand, 0C1 black garlic was the most antigenotoxic substance. The in vivo longevity assays showed significant extension of lifespan at some concentrations of white and 0C1and 1C2 black garlic. The in vitro experiments showed that all of the garlic samples induced a decrease in leukemia cell growth. However, no type of garlic was able to induce proapoptotic internucleosomal DNA fragmentation. Taking into account the physicochemical and biological data, black garlic could be considered a potential functional food and used in the preventive treatment of age-related diseases. In addition, our findings could be relevant for black-garlic-processing agrifood companies, as the economical and timing costs can significantly be shortened from 45 to 13 days of aging.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF STEMS OF THE PLANT TAGETES ERECTA L. (FAMILY-ASTERACEAE)

2018 ◽  
Vol 10 (4) ◽  
pp. 64
Author(s):  
Jafar Ali ◽  
Dibyendu Shil
Keyword(s):  
Antimicrobial Activity ◽  
Microbial Activity ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Tagetes Erecta ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Phytochemical Constituents ◽  
Tagetes Erecta L

Objective: To estimate the anti-microbial activity of ethanolic extract of Tagetes erecta L. (family-Asteraceae) in association with phytochemical analysis.Methods: The ethanolic extract of the stems of TageteseErecta L. was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activity of ethanolic extract of has been carried out against one Gram positive bacteria-Staphylococcus aureus and one Gram negative bacteria-Escherichia coli, and the antifungal activity of the plant extract was evaluated on Candida albicans species. The testing was done by the disc diffusion method to visualize the zone of inhibition of ethanolic extract was compared with that of standard drug i.e., Ciprofloxacin for anti-bacterial activity and Terbinafine for anti-fungal activity. The MIC was determined by the microdilution method (Cup-plate method).Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the stems of Tagetes erecta L. Various phytochemical analysis confirmed the presence of alkaloids, saponin glycosides, flavonoids, phenol, proteins and amino acids, cardiac glycosides, steroids, tanin and phenolic compounds. The anti-microbial activity of the ethanolic extract of the plant showed significant results against all three of the test organisms in compare to the standard drugs.Conclusion: The present study concluded that ethanolic extract of-of the stems of Tagetes erecta L. Proved the existence of phytochemical constituents. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards and it can be useful for the development of the antibiotics.

Novel Calcium Aminoclay (CaAC)-Vitamin C Hybrid: In-Vitro Cytotoxicity Assessment in HaCaT Cells and In-Vivo Embryotoxicity Assay in Zebrafish

2021 ◽  
Vol 21 (7) ◽  
pp. 3667-3672
Author(s):  
Vinh Van Tran ◽  
Vu Khac Bui Hoang ◽  
Hang-Suk Chun ◽  
Ju-Young Moon ◽  
Young-Chul Lee
Keyword(s):  
Vitamin C ◽  
Cell Viability ◽  
Hacat Cell ◽  
In Vitro Cytotoxicity ◽  
Hatching Rate ◽  
Hacat Cells ◽  
Promising Candidate ◽  
Cytotoxicity Assessment

Vitamin C (VC) is well-known as a hydrophilic antioxidant commonly used in cosmeceutical formulations due to its protection and maintenance of youthful skin. Aminoclay (AC), a synthetic organic-nanoclay, has shown great potential for delivery of VC. However, the practical cosmeceutical applications of aminoclay for delivery of VC are severely limited due to the paucity of reported research on its cytotoxicity to human skin. Therefore, in the present study, we evaluated the biosafety of a calcium aminoclay-vitamin C (CaAC-VC) hybrid through an In-Vitro cytotoxicity assessment in HaCaT cells and an In-Vivo embryotoxicity assay in zebrafish. HaCaT cell viability and changes in the morphology and hatching rate of the zebrafish were investigated. The results indicated that the CaAC-VC hybrid showed a lower cytotoxicity relative to pure VC and that as such, it should be considered to be a promising candidate for VC-delivery applications.

scholarly journals Definition and Measurement of Follicle Stimulating Hormone

2000 ◽  
Vol 21 (1) ◽  
pp. 5-22 ◽  
Author(s):  
Matthew P. Rose ◽  
Rose E. Gaines Das ◽  
Adam H. Balen
Keyword(s):  
Single Molecule ◽  
Early Recognition ◽  
Biological Activities ◽  
World Health ◽  
In Vitro Assays ◽  
Assay Method ◽  
Endocrine Activity ◽  
Health Organization

Abstract FSH has a key role in the development and function of the reproductive system and is widely used both diagnostically and therapeutically in developmental and reproductive medicine. The accurate measurement of FSH levels, in patients for diagnosis and monitoring and in therapeutic preparations for clinical use, is essential for safe and successful treatment. Historically, FSH was defined on the basis of classical in vivo endocrine activity, and early therapeutic preparations were calibrated using in vivo bioassays. There was early recognition that reference preparations were required for calibration if the results from different laboratories were to be comparable. In response to the perceived need, the World Health Organization established the first standard for such preparations in 1959. Subsequent developments in biotechnology have led to recognition that there is no single molecule that can be uniquely defined as FSH, and that FSH can induce a range of biological activities. Several highly purified standards for FSH are now available, but discontinuity and heterogeneity of estimates of FSH activity in terms of these standards made using in vitro assays and binding assays have been noted. It is thus essential that any measurement of FSH include specification both of the standard with which the measured FSH is compared and the assay method used for that comparison.

Two zinc-aminoclays’ in-vitro cytotoxicity assessment in HeLa cells and in-vivo embryotoxicity assay in zebrafish

2017 ◽  
Vol 137 ◽  
pp. 103-112 ◽  
Author(s):  
Hang-Suk Chun ◽  
Duckshin Park ◽  
Song Eun Lim ◽  
Kwang-Hun Jeong ◽  
Ji-Seon Park ◽  
...  
Keyword(s):  
Hela Cells ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Assessment

scholarly journals Toxicological and Epigenetic Studies of Two Types of Ale Beer, Tyrosol and Iso-Alpha Humulone

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 485
Author(s):  
Tania Merinas-Amo ◽  
Rocío Merinas-Amo ◽  
Rafael Font ◽  
Mercedes del Río Celestino ◽  
Ángeles Alonso-Moraga
Keyword(s):  
Cancer Cell ◽  
Biological Activities ◽  
Methylation Status ◽  
Life Extension ◽  
In Vitro Assays ◽  
Antimutagenic Activity ◽  
Tumor Cell Death ◽  
Beer Consumption

Although many benefits drawn from beer consumption are claimed, the epidemiological records are contradictory with respect to cancer prevention. The purpose of this study was to investigate the possible health-related activities involving genome safety and the ageing processes of two types of lyophilised ale beers (blond and stout), as well as two of their bioactive compounds (tyrosol and iso-alpha humulone). A multipurpose trial set of in vivo toxicity, antitoxicity, mutagenicity, antimutagenicity, lifespan and healthspan assays using Drosophila melanogaster were used. In parallel, several in vitro assays were designed using the cancer cell line HL-60 in order to establish the possible chemopreventive activity of the selected substances, where epigenetic modulation of DNA methylation changes, clastogenic activity and tumour cell inhibition growth were evaluated. The safety of the four substances was confirmed: lyophilised blond ale beer (LBAB), lyophilised stout ale beer (LSAB), tyrosol and iso-alpha humulone were neither toxic nor genotoxic. Moreover, all substances, except tyrosol, revealed the ability to protect individual genomes against oxidative radicals and to exert antimutagenic activity against the genotoxin hydrogen peroxide. With respect to the degenerative process indicators of lifespan and healthspan, tyrosol was the only compound that did not exert any influence on the life extension of Drosophila; LBAB induced a significant lifespan extension in D. melanogaster; LSAB and its distinctive compound iso-alpha humulone induced a reduction in longevity. The in vitro assays showed the cytotoxic activity of LBAB, LSAB and tyrosol against HL-60 cells. Moreover, proapoptotic DNA fragmentation or DNA strand breakage was observed for both types of beers and iso-alpha humulone at different concentrations. Furthermore, the lyophilised ale beers and tyrosol exhibited an increasing genome-wide methylation status, while iso-alpha humulone exhibited a demethylation status in repetitive cancer cell sequences. Although the biological activities assigned to beer consumption cannot be linked to any specific molecule/element due to the complexity of the phenolic profile, as well as the multifactor brewing process, the results obtained let us propose lyophilised ale beers as safe potential nutraceutical beverages when consumed in moderate amounts. The prevention of toxicity and genetic oxidative damage, as well as the induction of tumor cell death and modulation of the methylation status, are the key activities of beer that were shown in the present research.

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