scholarly journals In Vitro Alpha-Amylase and Alpha-Glucosidase Inhibitory Activity and In Vivo Antidiabetic Activity of Withania frutescens L. Foliar Extract

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 293
Author(s):  
Hamza Mechchate ◽  
Imane Es-safi ◽  
Abdelhadi Louba ◽  
Ali S. Alqahtani ◽  
Fahd A. Nasr ◽  
...  
Keyword(s):  
Local Population ◽  
Fasting Blood Glucose ◽  
Continuous Treatment ◽  
Alpha Amylase ◽  
Antidiabetic Activity ◽  
In Vitro Assays ◽  
Diabetic Mice ◽  
Alpha Glucosidase

Withania frutescens L. is a wild perennial woody plant used by the local population for diverse therapeutic purposes. This work aims to study for the first time the potential inhibitory effect of this plant hydroethanolic extract on α-amylase and α-glucosidase activities using in vitro methods and its antidiabetic and antihyperglycemic activities using alloxan-induced diabetic mice as a model for experimental diabetes. Two doses were selected for the in vivo study (200 and 400 mg/kg) and glibenclamide, a well-known antidiabetic drug (positive control) in a subacute study (28 days) where the antihyperglycemic activity was also assessed over a period of 12 h on diabetic mice. The continuous treatment of diabetic mice with the extract of Withania frutescens for 4 weeks succeeded to slowly manage their high fasting blood glucose levels (after two weeks), while the antihyperglycemic test result revealed that the extract of this plant did not control hyperglycemia in the short term. No toxicity signs or death were noted for the groups treated with the plant extract, and it shows a protective effect on the liver and kidney. The in vitro assays demonstrated that the inhibition of alpha-amylase and alpha-glucosidase might be one of the mechanisms of action exhibited by the extract of this plant to control and prevent postprandial hyperglycemia. This work indicates that W. frutescens have an important long term antidiabetic effect that can be well established to treat diabetes.

scholarly journals Assessment of In Vitro Antidiabetic Potential of Purified Anthocyanin Extract from Floral Petals of Wild Balsam Species

2020 ◽  
Vol 10 (3) ◽  
pp. 31-35
Author(s):  
R ARATHY ◽  
K MURUGAN ◽  
KV DINESH BABU ◽  
GS MANOJ
Keyword(s):  
International Diabetes Federation ◽  
Inhibitory Effect ◽  
Alpha Amylase ◽  
Herbal Remedies ◽  
Antidiabetic Activity ◽  
Public Health Issue ◽  
Impatiens Balsamina ◽  
Alpha Glucosidase

Diabetes is a notorious and growing clinical and public health issue. The International Diabetes Federation assumes that 592 million had diabetes by 2035 and that by 2040 the number will increase to 642 million. Cardiovascular corollary accounts for four million deaths annually attributable to diabetes. Evidence reveals that certain glucose-lowering phytochemicals can improve vascular outcomes with type 2 diabetes, which, together with better understanding of using multiple therapies concurrently, offers opportunities for beneficial personalization of medication regimens. Anthocyanins are coloured pigments and are natural antioxidants. Keeping this in focus, this study was undertaken to evaluate the in vitro antidiabetic activity in the petals of wild Impatiens balsamina L. The anthocyanin was extracted from floral petals of wild balsam species and purified to homogeneity using chromatographic techniques. Evaluation of in vitro antidiabetic properties of anthocyanin extract revealed a dose-dependent increase in the inhibitory effect on the alpha-glucosidase (200 μg/ml) and alpha-amylase enzymes (500 μg/ml) and was comparable with the standard acarbose drug (189 μg/ml and 50 μg/ml). These results indicated that anthocyanin could be used as a source of functional food and nutraceuticals. This information from wild species will be useful in finding more potent antidiabetic principle from the natural resources for the clinical development of antidiabetic therapeutics. Future studies are planned to substantiate the antidiabetic power of anthocyanin using in vivo animal models. Keywords: Alpha amylase, alpha glucosidase, diabetes, herbal remedies, Impatiens balsamina L.

scholarly journals Potent Natural Inhibitors of Alpha-Glucosidase and Alpha-Amylase against Hyperglycemia in Vitro and in Vivo

2017 ◽  
Author(s):  
Jirawat Riyaphan ◽  
Chien-Hung Jhong ◽  
May-Jwan Tsai ◽  
Der-Nan Lee ◽  
Max K. Leong ◽  
...  
Keyword(s):  
Type Ii Diabetes ◽  
Herbal Medicines ◽  
Alpha Amylase ◽  
Type Ii ◽  
Reference Drug ◽  
Significant Difference ◽  
Natural Inhibitors ◽  
Alpha Glucosidase

The inhibition of alpha-glucosidase and alpha-amylase is one of clinic strategies for remedy the type II diabetes. Herbal medicines are reported to alleviate hyperglycemia. However, the constituents from those sources whether are targeted to the alpha-glucosidase and alpha-amylase still unexplored. This study attempted to select the compounds for efficacy of hypoglycemia via cellular and mouse levels. The results illustrated that the cytotoxicity in all tested compounds at various concentrations except the concentration of 16-hydroxy-cleroda-3,13-dine-16,15-olide (HCD) at 30 µM were not significant difference (p > 0.05) when compared with the untreated control. Acarbose (reference drug), Antroquinonol, Catechin, Quercetin, Actinodaphnine, Curcumin, HCD, Docosanol, Tetracosanol, Berberine, and Rutin could effectively inhibit the alpha-glucosidase activity of Caco-2 cells when compared with the control (maltose). The compounds (Curcumin, HCD, Tetracosanol, Antroquinonol, Berberine, Catechin, Actinodaphnine, and Rutin) could reduce blood sugar level at 30 min in tested mice. The effects of tested compounds on area under curve (AUC) were significant (p < 0.05) among Acarbose, Tetracosanol, Antroquinonol, Catechin, Actinodaphnine, and Rutin along with Berberine and Quercetin. In in vitro (alpha-glucosidase) with in vivo (alpha-amylase) experiments suggest that bioactive compounds can be a potential inhibitor candidate of alpha-glucosidase and alpha-amylase for the alleviation of type II diabetes.

scholarly journals Antidiabetic Activity ofPterospermum acerifoliumFlowers and Glucose Uptake Potential of Bioactive Fraction in L6 Muscle Cell Lines with Its HPLC Fingerprint

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Rathinavelusamy Paramaguru ◽  
Papiya Mitra Mazumder ◽  
Dinakar Sasmal ◽  
Venkatesan Jayaprakash
Keyword(s):  
Glucose Uptake ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diabetic Control ◽  
Antidiabetic Activity ◽  
Peripheral Tissues ◽  
Hplc Fingerprint

The present study was designed to estimate the detailed antidiabetic activity ofPterospermum acerifolium(L.) Willd flowers.In vitroalpha amylase inhibition study was carried out on 50% ethanol extract of flowers (PAFEE) and its various fractions. The active ethyl acetate fraction (PAFEF) was subfractionated into three subfractions (PAFE1, PAFE2, and PAFE3) and subjected to acute toxicity studies followed by antidiabetic screeningin vivoby streptozotocin-nicotinamide induced type II diabetes. Diabetic animals treated with PAFE2 (30 mg/kg) reduced the levels of fasting blood glucose, significantly (P<0.001) compared to that of diabetic control animals. Histological studies on drug treated groups did not show remarkable positive changes inβ-cells. PAFE2 showed32.6±1.93% glucose uptake over control and, in the presence of PI3K inhibitor wortmannin, declined to13.7±2.51%. HPLC analysis of PAFE2 reveals the presence of quercetin and apigenin as major constituents and both are inhibiting the glycogen phosphorylase enzyme in molecular modelling studies. The study evidenced strongly that the probable glucose lowering mechanism of action of active subfraction PAFE2 is by increasing the glucose uptake in peripheral tissues and by inhibition of gluconeogenesis.

scholarly journals In vitro, acute and subchronic evaluation of the antidiabetic activity of Atractylis flava Desf n-butanol extract in alloxan-diabetic rats

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mohamed Akram Melakhessou ◽  
Salah Eddine Marref ◽  
Naima Benkiki ◽  
Cherine Marref ◽  
Imene Becheker ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Plant Extract ◽  
Fasting Blood Glucose ◽  
Folk Medicine ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Oral Glucose ◽  
Butanol Extract

Abstract Background Diabetes mellitus is a serious complex multifactorial disorder that imposes huge health and economic burden on societies. Because the currently available medications have many drawbacks, it's important to look for alternative therapies. Medicinal plants utilized in folk medicine are ideal candidates. Therefore, this work assessed the antidiabetic action of n-butanol extract from the whole plant Atractylis flava Desf (BEAF). These ethnomedicinal properties of BEAF were scientifically validated using in vitro and in vivo assays. In vitro antidiabetic effect of the BEAF was conducted using α-Glucosidase and α-Amylase assays. While the antihyperglycemic activity was assessed using two rat models: Alloxan-induced diabetic rats and oral glucose challenged rats. Experimental diabetes was induced by a single intraperitoneal injection of alloxan at a dose of 150 mg/kg and animals with fasting blood glucose levels (BGL) > 200 mg/dL were considered diabetic. Glibenclamide (5 mg/kg) was used as a typical drug. Results The BEAF at all tested dose levels (100, 250, and 500 mg/kg) showed a significant decrease in blood glucose level in all the two animal models. Besides, the plant extract exhibited a potent inhibitory effect on α-Amylase and α-Glucosidase activity at a concentration of 1000 µg/mL with 76.17% and 89.37%, respectively. Conclusion BEAF exerts in vitro and in vivo antidiabetic effects, these results suggest that the plant extract can be a therapeutic resource in the treatment of diabetes and hyperlipidemia.

scholarly journals BIOASSAY-GUIDED EVALUATION OF FICUS SEMICORDATA FOR ANTIDIABETIC ACTIVITY

2017 ◽  
Vol 9 (3) ◽  
pp. 71 ◽  
Author(s):  
Virender Kaur ◽  
Kumud Upadhyaya ◽  
Milind Pande
Keyword(s):  
Diabetes Mellitus ◽  
Early Stage ◽  
Ethanol Extract ◽  
Postprandial Hyperglycemia ◽  
Alpha Amylase ◽  
Antidiabetic Activity ◽  
Biological Studies

Objective: The early stage of diabetes mellitus type 2 is associated with postprandial hyperglycemia. The therapeutic approach involved in the treatment of type 2 diabetes mellitus is the use of agents that can decrease postprandial hyperglycemia by inhibiting carbohydrate digesting enzymes. In an effort of identifying herbal drugs which may become useful in the prevention or mitigation of diabetes, the antidiabetic activity of Ficus semicordata (FS) and its constituents were studied. The present study was undertaken in part to identify the potent antihyperglycemic fraction from the ethanol extract of the plant, using bioassay guided evaluation.Methods: The ethanol extract of Ficus semicordata were fractionated to obtain chloroform, ethyl acetate, n-butanol and ethanol extracts which were tested for alpha-amylase, alpha-glucosidase inhibitory, properties. Further fractionation of the more active ethanol fraction yielded isolates FS-1 and FS-2 which were tested for in vivo antidiabetic activity using Streptozotocin (STZ)-induced diabetic rats.Results: Ethanol extract from leaves of the plant showed notable alpha-amylase (IC50 = 3.352µg/ml and alpha-glycosidase inhibitory activity (IC50= 3.448µg/ml) as compared to standard acarbose (IC50 = 3.175µg/ml. Subfraction FS-1 and FS-2 which were tested for in vivo antidiabetic activity using acute STZ-induced diabetic rats significantly (*p<0.05, **p<0.01, *** p<0.001) reduced blood glucose level.Conclusion: The Ficus semicordata plant extracts and the fractionated components could be used as a natural antidiabetic after comprehensive in vitro and in vivo biological studies.

scholarly journals Preliminary Analysis of the Antidiabetic Activity of Indonesian Polyherbal Formulation

2021 ◽  
Vol 21 (3) ◽  
pp. 219
Author(s):  
Syamsudin Abdillah ◽  
Apriala Gita Lestari ◽  
Priskila Monika ◽  
Wahono Sumaryono ◽  
Kartiningsih Hisyam
Keyword(s):  
Stevia Rebaudiana ◽  
Inhibitory Effect ◽  
Antidiabetic Activity ◽  
Smallanthus Sonchifolius ◽  
Polyherbal Formulation ◽  
Diabetes In Mice ◽  
Ethanol Extracts ◽  
Alpha Glucosidase

This study aims to prepare polyherbal formulations and also analyze the antidiabetic potentials of the prepared polyherbal in animals. The ethanol extracts of the leaves of <em>Smallanthus sonchifolius</em>, <em>Stevia rebaudiana, Syzygium polyanthum</em>, and <em>Camellia sinensis</em> were used in this study. The extracts of the aforementioned plants were prepared in the ratio of 2:1:1:1. The formulation was tested for antidiabetic activity <em>in vitro</em> through the inhibition of alpha-glucosidase and <em>in vivo</em> using alloxan-induced diabetes in mice. The polyherbal has an inhibitory effect with the IC<sub>50</sub> value of 26.23 μg/mL compared to acarbose (control) was 17.02 μg/mL. The diabetic animals were observed to show an obvious decline in glucose level when compared with control (P&lt; 0.001) after treatment.

scholarly journals In vivo and in vitro antidiabetic potential of Taraxacum officinale root extracts

2020 ◽  
Vol 33 (3) ◽  
pp. 168-175
Author(s):  
Lana YM. Juee ◽  
Alaadin M. Naqishbandi
Keyword(s):  
Glucose Tolerance ◽  
Glucose Uptake ◽  
Aqueous Extract ◽  
Taraxacum Officinale ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Diabetic Mice ◽  
Phytochemical Study

AbstractTaraxacum officinale F.H. Wigg (Asteraceae) root is traditionally used to treat diabetes, dyspepsia, heartburn, anorexia and hepatitis. In this work, petroleum ether, chloroform, methanol and aqueous extracts of T. officinale root were evaluated for their antidiabetic activity in normoglycemic and alloxan-induced diabetic mice at two concentrations (200 and 400 mg/kg) using antidiabetic and subcutaneous glucose tolerance tests. Herein, in vitro glucose uptake assay was performed using HepG2 and 2-NDBG, while LC-MS/MS was employed for the phytochemical study of the main active constituents in the active extract. In the experiments, T. officinale root aqueous extract (400 mg/kg) showed a significant decrement in blood glucose level (62.33%, p ≤0.05), while other extracts (p >0.05) showed insignificant activity – in alloxan-induced diabetic mice with no apparent effect on the normoglycemic model. The extracts also showed an insignificant reduction in glucose levels (p >0.05) in the subcutaneous glucose tolerance test. However, a significant glucose uptake enhancement (149.6724%, p ≤0.05) was exhibited by the aqueous extract. Phytochemical study of the aqueous extract showed higher total phenolic than total flavonoid content, in which chlorogenic acid, protocatechuic acid, and luteolin-7-glucoside were identified.

scholarly journals Exploration of Antidiabetic Activity of Stephania japonica Leaf Extract in Alloxan-Induced Swiss Albino Diabetic Mice

2019 ◽  
pp. 1-12
Author(s):  
Md. Dobirul Islam ◽  
Syeda Farida Akter ◽  
Md. Amirul Islam ◽  
Md. Salim Uddin
Keyword(s):  
Gallic Acid ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Diabetic Mice ◽  
Mice Model ◽  
Leaf Extracts ◽  
Alternative Source ◽  
Dry Extract

Aims: Presently the medicinal world is rapidly turning more on the therapeutic health benefits of natural product and medicinal plants in the management of major crucial disease and their complications. Medicinal plant, Stephania japonica has been studied for exploring antidiabetic potentiality as an alternative source of medicine against the global threat of Diabetes mellitus (DM). Methods: The extraction of S. japonica leaf was carried out by acetone and ethanol. Phytochemical screening and quantitative analysis of S. japonica leaf extracts were evaluated through chemically forming characterized color formation and calibration method respectively, by using standard reference substances (ascorbic acid, gallic acid, and quercetin) to assess the probable compounds present in the extract. Anti-diabetic potentiality of highest phytochemicals containing two extracts were investigated in in vitro as a ⍺-amylase inhibitors and in vivo through alloxan-induced Swiss albino diabetes mice model. Results: Alkaloids, carbohydrates, steroids, flavonoids, resins, saponins, tannins and coumarins were present in the leaf extracts. The estimated amount of total phenolics, flavonoids, flavonols and proanthrocyanidins contents of acetone and ethanol extract were 92.12±0.64 and 56.54±1.05 mg of gallic acid equivalent (GAE)/gm of dry extract, 66.02±1.42 and 46.17±0.54 mg of catechin equivalent (CAE)/gm of dry extract, 7.05±0.108 and 5.26±0.083 mg of quercetin equivalent (QUE)/gm of dry extract, 35.19±0.67 and 9.55±1.11 mg CAE/gm of dry extract, respectively. In 3, 5-dinitrosalicylic acid method, acetone and ethanol extract showed α-amylase inhibition of 51.02% and 46.62%, respectively at the concentration of 1000 µg/mL whereas in starch iodine color assay, acetone and ethanol extract showed inhibition of 57.32% and 52.12%, respectively at the concentration of 800 µg/mL. In contrast, both of the leaf extracts significantly (p<0.05 to p<0.001) improved the lipid profile parameters, blood glucose level and serum hepatic marker proteins in alloxan-induced diabetic mice. Conclusion: The present study strongly concluded that S. japonica leaf extracts process potent antidiabetic potentiality that might be significance for the management of diabetes and its complications.

scholarly journals Antidiabetic activity, alpha-amylase, and alpha-glucosidase inhibitory effect of Tradescantia spathacea Swartz extract

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1594-1599
Author(s):  
Nagaraju Jesetti ◽  
Rajasekhar Reddy Alavala ◽  
Subhakar Raju Rapaka ◽  
Umasankar Kulandaivelu ◽  
Koteswara Rao GSN
Keyword(s):  
Methanolic Extract ◽  
Amylase Activity ◽  
Inhibitory Effect ◽  
Antidiabetic Activity ◽  
Oral Glucose ◽  
Diabetic Patients ◽  
Diabetic Model ◽  
Alpha Glucosidase

Tradescantia spathacea Swartz belongs to the genus  Commelinaceae, a tropical tree used in many countries as an herbal drug for the care of diabetic patients. The aim of this study was to examine anti-diabetic activity of the Tradescantia spathacea Swartz methanolic extract (METSW) and the in-vitro activity of α-amylase, and α-glucosidase was carried out.  METSW compared with acarbose inhibition of the α-amylase and α-glucosidase enzyme, METSW exhibited IC50 less than 100μg/mL would be considered as healthy. The METSW showed IC50 66.22 ± 0.52μg/mL α-amylase activity, acarbose revealed an IC50 of 83.25 ± 1.28μg/mL. METSW demonstrated IC50 levels of  85.37 ± 0.72 μg/mL (y= 0.095x+41.89) inhibition of  the α-Glucosidase enzymes.  METSW at 400 mg/kg greatly decreased the region under the blood glucose level curve in a typical rat test for oral glucose tolerance. The single dose of the extract decreased dramatically from  211 mg/dl to 89.22 mg/dl at 400 mg/kg  METSW in the alloxan induced diabetic model. METSW possesses strong antidiabetic activity in vivo  and in vitro. Besides, the extract has also been shown to have a significant inhibitory activity of α-amylase and α-glucosidase which may lead to its anti-hyperglycemic function when used in diabetic patients. 

scholarly journals Anti-diabetic activity of Clerodendrum paniculatum leaves by In-vitro, In-vivo and Ex-vivo methods

2021 ◽  
Vol 16 (1) ◽  
pp. 211-118
Author(s):  
Venkatesh S ◽  
Aswani K ◽  
Asheena Asharaf V V ◽  
Anjitha P ◽  
Suresh A ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Ex Vivo ◽  
Soxhlet Extraction ◽  
Chloroform Extract ◽  
Alpha Amylase ◽  
Standard Drug ◽  
Ic50 Values ◽  
Alpha Glucosidase

The present study has been undertaken to evaluate the in-vitro, in-vivo and ex-vivo anti-diabetic activity of leaves of chloroform extract Clerodendrum paniculatum (CECP). The extract was prepared by soxhlet extraction. Phytochemical screening indicates the presence of flavonoids, phenols, carbohydrates etc. The anti-diabetic activity of extract was studied by in-vitro (alpha amylase inhibition and alpha glucosidase inhibition assay), in-vivo (streptozotocin induced diabetes) and ex-vivo (glucose uptake by rat hemi - diaphragm method). For in-vitro studies, the inhibitory action of CECP was compared with standard drug Acarbose. The IC50 values of CECP for alpha amylase and alpha glucosidase was found to be 158.396 µg/ml and 113.122 µg/ml respectively and the extract shows significant anti-diabetic activity. For in-vivo and ex-vivo studies Glibenclamide was used as a standard drug to compare the blood glucose level and uptake of glucose was calculated. The results obtained from the study indicate that both 200 mg/kg and 400 mg/kg of CECP showed significant anti-diabetic activity. The 400 mg/kg of CECP showed better activity when compared to 200 mg/kg of the extract. The glucose uptake study was performed by isolated rat hemidiaphragm method. The hemi diaphragm obtained from the rats treated with both the doses of CECP showed significant glucose uptake.

Sign in / Sign up

Export Citation Format

Share Document