scholarly journals Oleocanthal Quantification Using 1H NMR Spectroscopy and Polyphenols HPLC Analysis of Olive Oil from the Bianchera/Belica Cultivar

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 242
Author(s):  
Martina Starec ◽  
Antonella Calabretti ◽  
Federico Berti ◽  
Cristina Forzato
Keyword(s):  
1H Nmr ◽  
Total Phenolic ◽  
Health Claim ◽  
Virgin Olive Oils ◽  
Beneficial Effects ◽  
Olive Oils ◽  
Commission Regulation ◽  
Eu Commission ◽  
Hplc Analyses ◽  
The Eu

The cultivar Bianchera is an autochthonous variety from the eastern part of northern Italy, but it is also cultivated in the Slovenian and Croatian peninsula of Istria where it is named Belica (Slovenia) and Bjelica (Croatia). The properties of oleocanthal, a natural anti-inflammatory ibuprofen-like compound found in commercial monocultivar extra virgin olive oils, were determined by means of both quantitative 1H NMR (qNMR) and HPLC analyses, where qNMR was identified as a rapid and reliable method for determining the oleocanthal content. The total phenolic content (TPC) was determined by means of the Folin–Ciocalteau method and the major phenols present in the olive oils were also quantified by means of HPLC analyses. All these analyses confirmed that the cultivar Bianchera was very rich in polyphenols and satisfied the health claim provided by the EU Commission Regulation on the polyphenols content of olive oils and their beneficial effects on human health.

scholarly journals Detection of olive oils authenticity by determination of their sterol content using LC/GC

2013 ◽  
Vol 19 (No. 3) ◽  
pp. 97-103 ◽  
Author(s):  
I. Bohačenko ◽  
Z. Kopicová
Keyword(s):  
Packed Column ◽  
Gradient Elution ◽  
Unsaponifiable Fraction ◽  
Virgin Olive Oils ◽  
Olive Oils ◽  
Modified Method ◽  
Commission Regulation ◽  
Preparative Lc ◽  
The Eu

The content of selected sterols as declared in the EU Commission Regulation was used to prove the authenticity of olive oils. A modified method using the preparative LC with silica gel packed column and gradient elution with three mixtures of hexane and diethyl ether was used to separate undesirable interfering compounds in the unsaponifiable fraction before the determination of sterols using GC. Model experiments based on the determination of D-7-stigmastenol and campesterol (addition of sunflower and soybean oils), or brassicasterol (addition of rapeseed oil) were used to verify that this method is capable of identifying adulteration of olive oils by additions of sunflower, soybean or rapeseed oils. An elevated content of these marker sterols, in comparison with their permitted contents, enables the identification of an addition of 5–10% of the above oils to the olive oil. This method was also used to evaluate the authenticity of five samples of olive oils from the SIAL exhibition (Paris) and ten samples of virgin olive oils obtained on thePrague markets. It was revealed that none of the samples showed the signs of adulteration.

scholarly journals Traceability of “Tuscan PGI” Extra Virgin Olive Oils by 1H NMR Metabolic Profiles Collection and Analysis

Metabolites ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 60 ◽  
Author(s):  
Chiara Roberta Girelli ◽  
Laura Del Coco ◽  
Samanta Zelasco ◽  
Amelia Salimonti ◽  
Francesca Luisa Conforti ◽  
...  
Keyword(s):  
Olive Oil ◽  
1H Nmr ◽  
Large Scale ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Added Value ◽  
Metabolic Profiles ◽  
The European Union ◽  
Virgin Olive Oils ◽  
Olive Oils

According to Coldiretti, Italy still continues to hold the European Quality record in extra virgin olive oils with origin designation and protected geographical indication (PDO and PGI). To date, 46 Italian brands are recognized by the European Union: 42 PDO and 4 PGI (Tuscan PGI, Calabria PGI; Tuscia PGI and PGI Sicily). Specific regulations, introduced for these quality marks, include the designation of both the geographical areas and the plant varieties contributing to the composition of the olive oil. However, the PDO and PGI assessment procedures are currently based essentially on farmer declarations. Tuscan PGI extra virgin olive oil is one of the best known Italian trademarks around the world. Tuscan PGI varietal platform is rather wide including 31 specific olive cultivars which should account for at least 95% of the product. On the other hand, while the characteristics of other popular Italian extra virgin olive oils (EVOOs) cultivars from specific geographical areas have been extensively studied (such as those of Coratina based blends from Apulia), little is still known about Tuscan PGI EVOO constituents. In this work, we performed, for the first time, a large-scale analysis of Tuscan PGI monocultivar olive oils by 1H NMR spectroscopy and multivariate statistical analyses (MVA). After genetic characterization of 217 leaf samples from 24 selected geographical areas, distributed all over the Tuscany, a number of 202 micro-milled oil samples including 10 PGI cultivars, was studied. The results of the present work confirmed the need of monocultivar genetically certified EVOO samples for the construction of 1H-NMR-metabolic profiles databases suitable for cultivar and/or geographical origin assessment. Such specific PGI EVOOs databases could be profitably used to justify the high added value of the product and the sustainability of the related supply chain.

Determination of 1,2/1,3-diglycerides in Sicilian extra-virgin olive oils by 1H-NMR over a one-year storage period

2016 ◽  
Vol 31 (7) ◽  
pp. 822-828 ◽  
Author(s):  
Andrea Salvo ◽  
Archimede Rotondo ◽  
Giovanna Loredana La Torre ◽  
Nicola Cicero ◽  
Giacomo Dugo
Keyword(s):  
1H Nmr ◽  
Storage Period ◽  
Virgin Olive Oils ◽  
Olive Oils ◽  
One Year

Characterisation of the Turkish and Slovenian extra virgin olive oils by chemometric analysis of the presaturation 1H NMR spectra

LWT ◽  
2018 ◽  
Vol 92 ◽  
pp. 10-15 ◽  
Author(s):  
İbrahim Sani Özdemir ◽  
Çağdaş Dağ ◽  
Damjan Makuc ◽  
Erdal Ertaş ◽  
Janez Plavec ◽  
...  
Keyword(s):  
1H Nmr ◽  
Nmr Spectra ◽  
Chemometric Analysis ◽  
1H Nmr Spectra ◽  
Virgin Olive Oils ◽  
Olive Oils

scholarly journals Extraction, Analysis, and Antioxidant Activity Evaluation of Phenolic Compounds in Different Italian Extra-Virgin Olive Oils

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3249 ◽  
Author(s):  
Chiara Fanali ◽  
Susanna Della Posta ◽  
Alessandra Vilmercati ◽  
Laura Dugo ◽  
Marina Russo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Limit Of Detection ◽  
Total Concentration ◽  
Total Phenolic ◽  
Virgin Olive Oils ◽  
Olive Oils ◽  
Italian Regions ◽  
Compound Concentration

The analysis of phenolic compounds in extra virgin olive oils was carried out by high-performance liquid chromatography utilizing photodiode array and mass spectrometry detectors. The chromatographic profile of thirty samples from four Italian Regions highlighted the presence of secoiridoids, phenolic alcohols, flavonoids, and phenolic acid classes. A similar qualitative profile was observed with some differences in peak area and fifteen compounds were tentatively identified. Quantitative analysis was performed by UV detection considering eight standard phenolic compounds. The chromatographic method, after optimization, was validated studying some parameters, e.g., intra-day and inter-day retention time precision, limit of detection, limit of quantification, and linearity. Recovery of the method was performed achieving good results (10 and 50 g·g−1 with recovery of 72.9–92.1% (w/w) and 79.1–102.8% (w/w), respectively). In all samples secoiridoids were the main compounds ranging from 85 to more than 99% (w/w) of the total concentration of detected phenolic compounds while phenolic acids accounted for the lowest percentage (0.1–0.6%, w/w). Finally, total concentration of phenolic compounds and antioxidant activity were determined with different chemical assays. A good and significant correlation among total phenolic compound concentration and antioxidant activity was observed. A significant different phenolic compound concentration and antioxidant activity was determined between samples from Puglia and Sicily. This was studied performing statistical analysis by one-way analysis of variance (ANOVA) followed by Bonferroni post-hoc test.

scholarly journals Phenolic Profile and Antioxidant Activity of Italian Monovarietal Extra Virgin Olive Oils

Antioxidants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 161 ◽  
Author(s):  
Carmine Negro ◽  
Alessio Aprile ◽  
Andrea Luvisi ◽  
Francesca Nicolì ◽  
Eliana Nutricati ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Profile ◽  
Virgin Olive Oils ◽  
Olive Oils ◽  
Ic50 Values ◽  
Phenolic Profiles ◽  
Trolox Equivalent ◽  
Phenolic Substances

In the last years, the interest in Italian monovarietal oils has increased due to their specific organoleptic qualities. Extra virgin olive oils (EVOOs) are rich in phenolic compounds, secondary metabolites well known and studied for their nutraceutical properties. However, among EVOOs, there is great variability in phenolic composition due to the origin, the production technique, and mainly, the genotype. The aim of this work was to evaluate the different phenolic profiles and the antioxidant activities of monovarietal oils. The results confirm this variability. In fact, the overall content of oleuropein varies up to four times between the different genotypes (from 33.80 to 152.32 mg/kg oil), while the oleocanthal content is significant only in two oils. The antioxidant activity, determined with 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) assays, is correlated with the content of total phenolic substances, with half maximal inhibitory concentration (IC50) values for the DPPH test ranging from 160 to 91 mg of oil, while the ORAC test shows values between 5.45 and 8.03 μmol Trolox equivalent (TE)/g oil.

Multivariate Analysis of 1H-NMR Spectra of Genetically Characterized Extra Virgin Olive Oils and Growth Soil Correlations

2011 ◽  
Vol 88 (10) ◽  
pp. 1463-1475 ◽  
Author(s):  
Paride Papadia ◽  
Laura Del Coco ◽  
Innocenzo Muzzalupo ◽  
Marco Rizzi ◽  
Enzo Perri ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
1H Nmr ◽  
Nmr Spectra ◽  
1H Nmr Spectra ◽  
Virgin Olive Oils ◽  
Olive Oils

scholarly journals The Potential of Apulian Olive Biodiversity: The Case of Oliva Rossa Virgin Olive Oil

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 369
Author(s):  
Giacomo Squeo ◽  
Roccangelo Silletti ◽  
Giacomo Mangini ◽  
Carmine Summo ◽  
Francesco Caponio
Keyword(s):  
Virgin Olive Oil ◽  
Monounsaturated Fatty Acids ◽  
Quality Parameters ◽  
Total Phenolic ◽  
Oil Accumulation ◽  
Virgin Olive Oils ◽  
Olive Oils ◽  
Minor Compounds ◽  
Few Data ◽  
High Level

In this study, the drupes and virgin olive oils extracted from the Oliva Rossa landrace are characterized. Oliva Rossa is an old landrace part of the autochthonous Apulian olive germplasm for which only few data have been reported till now. During the study, the maturity patterns of the drupes had been followed. Four samplings per year were planned, one every 14 days starting from the middle of October. The pigmentation index, the oil content and the total phenolic content of the drupes were measured. Simultaneously, virgin olive oils were extracted at the lab scale and analyzed for the fatty acid composition, the basic quality parameters and the content of minor compounds. The pigmentation pattern of the drupes was different among the years and, despite this trend, at the third sampling time the stage of maximum oil accumulation was always over. The extracted virgin olive oils had a medium to high level of oleic acid. With colder temperatures, a higher level of monounsaturated fatty acids, oleic/linoleic ratio and antioxidants was observed. The phenolic profile was dominated by 3,4-DPHEA-EDA and p-HPEA-EDA while the volatile profile by (E)-2-hexenal and 3-ethyl-1,5-octadiene.

scholarly journals Chemical and Biological Evaluation of the Oil and Seedcake from Seeds of a Greek Cardoon Cultivar as Potential Functional Vegetable Oil. Comparison with Sesame, Flaxseed and Extra Virgin Olive Oils

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2665
Author(s):  
Elisavet-Foteini Varvouni ◽  
Konstantia Graikou ◽  
Olga Gortzi ◽  
Antigoni Cheilari ◽  
Nektarios Aligiannis ◽  
...  
Keyword(s):  
Vegetable Oil ◽  
Seed Oil ◽  
Agricultural Waste ◽  
Biological Evaluation ◽  
Rich Source ◽  
Total Phenolic ◽  
Cynara Cardunculus ◽  
Virgin Olive Oils ◽  
Olive Oils ◽  
Cold Pressed

Cynara cardunculus L. is a plant of the Mediterranean basin, known since antiquity as a food and for its therapeutic properties. The needs of the 21st century for the utilization of agricultural waste has led to the study of the seed oil of a Greek cultivar of Cynara cardunculus (GCCC) as potential nutritional oil, as large amounts of cardoon seeds are discarded. The sterol and fatty acid profile of cold-pressed seed oil was examined by gas chromatography-mass spectrometry GC-MS and compared with that of solvent extraction. Total phenolic content was determined and compared with well-known and widely appreciated edible vegetable oils; while, additionally, the total lignan content and nutritional value of cold-pressed oil revealed it as a potential dietary candidate. Furthermore, the seedcake (residue of cold-pressed oil extraction) has been studied exerting it as a good source of phenolics. Both GCCC oil and seedcake were tested for their antioxidant and enzyme inhibitory activities exhibiting higher activity compared to the sesame, flaxseed and extra virgin olive oils. According to the results, Cynara seed oil was shown to be a rich source of ω-6/-9 fatty acids and phenolics, highlighting, indicating that it could be a promising health-promoting vegetable oil, while the seedcake was revealed as a rich source of bioactive compounds.

scholarly journals The Use of Electronic Nose as Alternative Non-Destructive Technique to Discriminate Flavored and Unflavored Olive Oils

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2886
Author(s):  
Nuno Rodrigues ◽  
Kevin Silva ◽  
Ana C. A. Veloso ◽  
José Alberto Pereira ◽  
António M. Peres
Keyword(s):  
Electronic Nose ◽  
Quality Parameters ◽  
Total Phenolic ◽  
Virgin Olive Oils ◽  
Phenolic Contents ◽  
Olive Oils ◽  
Classification Tool ◽  
Sensory Profiles ◽  
Non Destructive ◽  
Flavoring Agent

Cv. Arbequina extra virgin olive oils (EVOO) were flavored with cinnamon, garlic, and rosemary and characterized. Although flavoring significantly affected the physicochemical quality parameters, all oils fulfilled the legal thresholds for EVOO classification. Flavoring increased (20 to 40%) the total phenolic contents, whereas oxidative stability was dependent on the flavoring agent (a slight increase for rosemary and a decrease for cinnamon and garlic). Flavoring also had a significant impact on the sensory profiles. Unflavored oils, cinnamon, and garlic flavored oils had a fruity-ripe sensation while rosemary flavored oils were fruity-green oils. Fruit-related sensations, perceived in unflavored oils, disappeared with flavoring. Flavoring decreased the sweetness, enhanced the bitterness, and did not influence the pungency of the oils. According to the EU regulations, flavored oils cannot be commercialized as EVOO. Thus, to guarantee the legal labelling requirement and to meet the expectations of the market-specific consumers for differentiated olive oils, a lab-made electronic nose was applied. The device successfully discriminated unflavored from flavored oils and identified the type of flavoring agent (90 ± 10% of correct classifications for the repeated K-fold cross-validation method). Thus, the electronic nose could be used as a practical non-destructive preliminary classification tool for recognizing olive oils’ flavoring practice.

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