scholarly journals Tetracyclic and Pentacyclic Triterpenes with High Therapeutic Efficiency in Wound Healing Approaches

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5557
Author(s):  
Roxana Ghiulai ◽  
Oana Janina Roşca ◽  
Diana Simona Antal ◽  
Marius Mioc ◽  
Alexandra Mioc ◽  
...  
Keyword(s):  
Wound Healing ◽  
Molecular Mechanisms ◽  
Pharmaceutical Formulations ◽  
Complex Process ◽  
Common Skin ◽  
Ancient Times ◽  
Skin Conditions ◽  
Degrees Of Severity

Wounds are among the most common skin conditions, displaying a large etiological diversity and being characterized by different degrees of severity. Wound healing is a complex process that involves multiple steps such as inflammation, proliferation and maturation and ends with scar formation. Since ancient times, a widely used option for treating skin wounds are plant- based treatments which currently have become the subject of modern pharmaceutical formulations. Triterpenes with tetracyclic and pentacyclic structure are extensively studied for their implication in wound healing as well as to determine their molecular mechanisms of action. The current review aims to summarize the main results of in vitro, in vivo and clinical studies conducted on lupane, ursane, oleanane, dammarane, lanostane and cycloartane type triterpenes as potential wound healing treatments.

scholarly journals Microfluidic and Lab-on-a-Chip Systems for Cutaneous Wound Healing Studies

2021 ◽  
Author(s):  
Ghazal Shabestani Monfared ◽  
Peter Ertl ◽  
Mario Rothbauer
Keyword(s):  
Wound Healing ◽  
Molecular Mechanisms ◽  
Chronic Wounds ◽  
Cell Communication ◽  
Lab On A Chip ◽  
Tissue Level ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound

Cutaneous wound healing is a complex multi-stage process involving direct and indirect cell communication events with the aim of efficiently restoring the barrier function of the skin. One key aspect in cutaneous wound healing is associated with cell movement and migration into the physically, chemically and biologically injured area resulting in wound closure. Understanding the conditions under which cell migration is impaired and elucidating the cellular and molecular mechanisms that improve healing dynamics is therefore crucial in devising novel therapeutic strategies to elevate patient suffering, reduce scaring and eliminate chronic wounds. Following the global trend towards automation, miniaturization and integration of cell-based assays into microphysiological systems, conventional wound healing assays such as the scratch assay or cell exclusion assay have recently been translated and improved using microfluidics and lab-on-a-chip technologies. These miniaturized cell analysis systems allow precise spatial and temporal control over a range of dynamic microenvironmental factors including shear stress, biochemical and oxygen gradients to create more reliable in vitro models that resemble the in vivo microenvironment of a wound more closely on a molecular, cellular, and tissue level. The current review provides (a) an overview on the main molecular and cellular processes that take place during wound healing, (b) a brief introduction into conventional in vitro wound healing assays, and (c) a perspective on future cutaneous and vascular wound healing research using microfluidic technology.

scholarly journals Triclosan: An Update on Biochemical and Molecular Mechanisms

2019 ◽  
Vol 2019 ◽  
pp. 1-28 ◽  
Author(s):  
Mohammad A. Alfhili ◽  
Myon-Hee Lee
Keyword(s):  
Molecular Mechanisms ◽  
Therapeutic Effects ◽  
Molecular Pathways ◽  
Biochemical Alterations ◽  
Present Decade ◽  
Surgical Sutures ◽  
Molecular Aspects ◽  
Skin Conditions

Triclosan (TCS) is a synthetic, chlorinated phenolic antimicrobial agent commonly used in commercial and healthcare products. Items made with TCS include soaps, deodorants, shampoos, cosmetics, textiles, plastics, surgical sutures, and prosthetics. A wealth of information obtained from in vitro and in vivo studies has demonstrated the therapeutic effects of TCS, particularly against inflammatory skin conditions. Nevertheless, extensive investigations on the molecular aspects of TCS action have identified numerous adversaries associated with the disinfectant including oxidative injury and influence of physiological lifespan and longevity. This review presents a summary of the biochemical alterations pertaining to TCS exposure, with special emphasis on the diverse molecular pathways responsive to TCS that have been elucidated during the present decade.

scholarly journals Microfluidic and Lab-on-a-Chip Systems for Cutaneous Wound Healing Studies

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 793
Author(s):  
Ghazal Shabestani Monfared ◽  
Peter Ertl ◽  
Mario Rothbauer
Keyword(s):  
Wound Healing ◽  
Molecular Mechanisms ◽  
Chronic Wounds ◽  
Cell Communication ◽  
Lab On A Chip ◽  
Tissue Level ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound

Cutaneous wound healing is a complex, multi-stage process involving direct and indirect cell communication events with the aim of efficiently restoring the barrier function of the skin. One key aspect in cutaneous wound healing is associated with cell movement and migration into the physically, chemically, and biologically injured area, resulting in wound closure. Understanding the conditions under which cell migration is impaired and elucidating the cellular and molecular mechanisms that improve healing dynamics are therefore crucial in devising novel therapeutic strategies to elevate patient suffering, reduce scaring, and eliminate chronic wounds. Following the global trend towards the automation, miniaturization, and integration of cell-based assays into microphysiological systems, conventional wound healing assays such as the scratch assay and cell exclusion assay have recently been translated and improved using microfluidics and lab-on-a-chip technologies. These miniaturized cell analysis systems allow for precise spatial and temporal control over a range of dynamic microenvironmental factors including shear stress, biochemical and oxygen gradients to create more reliable in vitro models that resemble the in vivo microenvironment of a wound more closely on a molecular, cellular, and tissue level. The current review provides (a) an overview on the main molecular and cellular processes that take place during wound healing, (b) a brief introduction into conventional in vitro wound healing assays, and (c) a perspective on future cutaneous and vascular wound healing research using microfluidic technology.

scholarly journals Wound Healing Properties of Selected Natural Products

2018 ◽  
Vol 15 (11) ◽  
pp. 2360 ◽  
Author(s):  
Nurul Ibrahim ◽  
Sok Wong ◽  
Isa Mohamed ◽  
Norazlina Mohamed ◽  
Kok-Yong Chin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Natural Products ◽  
Repair Process ◽  
Impaired Wound Healing ◽  
Complex Process ◽  
Synthesis Properties ◽  
Medicinal Properties ◽  
Underlying Diseases

Wound healing is a complex process of recovering the forms and functions of injured tissues. The process is tightly regulated by multiple growth factors and cytokines released at the wound site. Any alterations that disrupt the healing processes would worsen the tissue damage and prolong repair process. Various conditions may contribute to impaired wound healing, including infections, underlying diseases and medications. Numerous studies on the potential of natural products with anti-inflammatory, antioxidant, antibacterial and pro-collagen synthesis properties as wound healing agents have been performed. Their medicinal properties can be contributed by the content of bioactive phytochemical constituents such as alkaloids, essential oils, flavonoids, tannins, saponins, and phenolic compounds in the natural products. This review highlights the in vitro, in vivo and clinical studies on wound healing promotions by the selected natural products and the mechanisms involved.

scholarly journals A Low Molecular Weight Hyaluronic Acid Derivative Accelerates Excisional Wound Healing by Modulating Pro-Inflammation, Promoting Epithelialization and Neovascularization, and Remodeling Collagen

2019 ◽  
Vol 20 (15) ◽  
pp. 3722 ◽  
Author(s):  
Yin Gao ◽  
Yao Sun ◽  
Hao Yang ◽  
Pengyu Qiu ◽  
Zhongcheng Cong ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Wound Healing ◽  
Hyaluronic Acid ◽  
Molecular Mechanisms ◽  
Healing Process ◽  
Low Molecular Weight ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound

Recent knowledge of the cellular and molecular mechanisms underlying cutaneous wound healing has advanced the development of medical products. However, patients still suffer from the failure of current treatments, due to the complexity of healing process and thus novel therapeutic approaches are urgently needed. Previously, our laboratories produced a range of low molecular weight hyaluronic acid (LMW-HA) fragments, where a proportion of the glucosamine moieties were chemically N-acyl substituted. Specifically, N-butyrylation results in anti-inflammatory properties in a macrophage system, and we demonstrate the importance of N-acyl substituents in modulating the inflammatory response of LMW-HA. We have set up an inter-institutional collaborative program to examine the biomedical applications of the N-butyrylated LMW-HA (BHA). In this study, the potentials of BHA for dermal healing are assessed in vitro and in vivo. Consequently, BHA significantly promotes dermal healing relative to a commercial wound care product. By contrast, the “parent” partially de-acetylated LMW-HA (DHA) and the re-acetylated DHA (AHA) significantly delays wound closure, demonstrating the specificity of this N-acylation of LMW-HA in wound healing. Mechanistic studies reveal that the BHA-mediated therapeutic effect is achieved by targeting three phases of wound healing (i.e., inflammation, proliferation and maturation), demonstrating the significant potential of BHA for clinical translation in cutaneous wound healing.

scholarly journals Mechanically activated ion channel Piezo1 modulates macrophage polarization and stiffness sensing

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Hamza Atcha ◽  
Amit Jairaman ◽  
Jesse R. Holt ◽  
Vijaykumar S. Meli ◽  
Raji R. Nagalla ◽  
...  
Keyword(s):  
Wound Healing ◽  
Immune Responses ◽  
Positive Feedback ◽  
Macrophage Activation ◽  
Molecular Mechanisms ◽  
Macrophage Polarization ◽  
Subcutaneous Implantation

AbstractMacrophages perform diverse functions within tissues during immune responses to pathogens and injury, but molecular mechanisms by which physical properties of the tissue regulate macrophage behavior are less well understood. Here, we examine the role of the mechanically activated cation channel Piezo1 in macrophage polarization and sensing of microenvironmental stiffness. We show that macrophages lacking Piezo1 exhibit reduced inflammation and enhanced wound healing responses. Additionally, macrophages expressing the transgenic Ca2+ reporter, Salsa6f, reveal that Ca2+ influx is dependent on Piezo1, modulated by soluble signals, and enhanced on stiff substrates. Furthermore, stiffness-dependent changes in macrophage function, both in vitro and in response to subcutaneous implantation of biomaterials in vivo, require Piezo1. Finally, we show that positive feedback between Piezo1 and actin drives macrophage activation. Together, our studies reveal that Piezo1 is a mechanosensor of stiffness in macrophages, and that its activity modulates polarization responses.

scholarly journals The use of chitosan-based biomaterials for the treatment of hard-healing wounds

2019 ◽  
Vol 73 ◽  
pp. 768-781
Author(s):  
Marta Kędzierska ◽  
Katarzyna Miłowska
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Healing Process ◽  
Medical Engineering ◽  
Fibrin Clot ◽  
The Body ◽  
Complex Process ◽  
Other Substances

Wound healing is a complex process that engages skin cells, the blood, the immune system and a number of circulating substances in the body. Infections, contamination of the wound or a vast area of damage complicate and delay the natural process of skin regeneration. The incidence of hard-to-heal wounds is an increasingly common problem, because they can significantly impair the quality of life of the patient. For this reason, it is extremely important to look for factors (drugs, dressings or other substances) that could accelerate and relieve wound healing. Among many compounds in the area of medical engineering interest, attention should be paid to natural polysaccharides, e.g. chitosan and alginate. This article is devoted to biomaterials that play an important role in the treatment of chronic wounds. These include the following: hydrogels, non-wovens, membranes and chitosan sponges as well as chitosan-alginate composites or chitosan composites combined with zinc oxide and nanosilver. The material, which has chitosan as a base, works on all stages of the healing process. Many in vitro, in vivo and clinical studies that provide the basis for using chitosan materials as a substitute for conventional bandages and dressings have been carried out. At the stage of hemostasis, it accelerates platelet aggregation and the formation of a fibrin clot. In the inflamed stage, they cause the proliferation of neutrophils and macrophages that cleanse the wound, releasing cytokines at the wound site. Studies have shown that chitosan mimics the native extracellular matrix, providing the optimal microenvironment for the wound.

scholarly journals Microcurrent Stimulation Triggers MAPK Signaling and TGF-β1 Release in Fibroblast and Osteoblast-Like Cell Lines

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1924
Author(s):  
Evangelia Konstantinou ◽  
Zoi Zagoriti ◽  
Anastasia Pyriochou ◽  
Konstantinos Poulas
Keyword(s):  
Wound Healing ◽  
Transforming Growth Factor Beta ◽  
Transcriptional Activation ◽  
Molecular Mechanisms ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Mapk Signaling ◽  
Tgf Β1

Wound healing constitutes an essential process for all organisms and involves a sequence of three phases. The disruption or elongation of any of these phases can lead to a chronic or non-healing wound. Electrical stimulation accelerates wound healing by mimicking the current that is generated in the skin after any injury. Here, we sought to identify the molecular mechanisms involved in the healing process following in vitro microcurrent stimulation—a type of electrotherapy. Our results concluded that microcurrents promote cell proliferation and migration in an ERK 1/2- or p38-dependent way. Furthermore, microcurrents induce the secretion of transforming growth factor-beta-1 (TGF-β1) in fibroblasts and osteoblast-like cells. Interestingly, transcriptomic analysis uncovered that microcurrents enhance the transcriptional activation of genes implicated in Hedgehog, TGF-β1 and MAPK signaling pathways. Overall, our results demonstrate that microcurrents may enhance wound closure through a combination of signal transductions, via MAPK’s phosphorylation, and the transcriptional activation of specific genes involved in the healing process. These mechanisms should be further examined in vivo, in order to verify the beneficial effects of microcurrents in wound or fracture healing.

The effects of polydeoxyribonucleotide on wound healing and tissue regeneration: a systematic review of the literature

2020 ◽  
Vol 15 (6) ◽  
pp. 1801-1821
Author(s):  
Maria T Colangelo ◽  
Carlo Galli ◽  
Stefano Guizzardi
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Molecular Mechanisms ◽  
Therapeutic Potential ◽  
Receptor Activation ◽  
Cochrane Central Register ◽  
Eligibility Criteria ◽  
Study Results

Aim: The present study evaluated the effects of polydeoxyribonucleotide (PDRN) on tissue regeneration, paying special attention to the molecular mechanisms that underlie its tissue remodeling actions to better identify its effective therapeutic potential in wound healing. Materials & methods: Strategic searches were conducted through MEDLINE/PubMed, Google Scholar, Scopus, Web of Science and the Cochrane Central Register of Controlled Trials, from their earliest available dates to March 2020. The studies were included with the following eligibility criteria: studies evaluating tissue regeneration, and being an in vitro, in vivo and clinical study. Results: Out of more than 90 articles, 34 fulfilled the eligibility criteria. All data obtained proved the ability of PDRN in promoting a physiological tissue repair through salvage pathway and adenosine A2A receptor activation. Conclusion: Up to date PDRN has proved promising results in term of wound regeneration, healing time and absence of side effects.

scholarly journals MODELS OF WOUND HEALING

2021 ◽  
Vol 10 (4) ◽  
pp. 64-71
Author(s):  
A. Yu. Tsibulevskiy ◽  
T. К. Dubovaya ◽  
I. A. Demyanenko
Keyword(s):  
Wound Healing ◽  
Large Scale ◽  
Laboratory Animals ◽  
Skin Wounds ◽  
Physiological Processes ◽  
Complex Process ◽  
History Of ◽  
Chronic Skin

Simulations of human disease in laboratory animals have continuously accompanied the history of medical discoveries and have helped researchers obtain useful information in medicine. The separation of physiological processes into their constituent components through modeling is one of the important parts of any research. Wound healing is a complex process. Using of models (in vitro, in vivo - on animals and humans) allowed large-scale research in this area, which significantly improved the knowledge of the healing mechanisms of both acute and chronic skin wounds. In parallel with the knowledge gained, the development of new wound treatments continues and the understanding of how to improve their care for the best results continues. Although in vitro and animal models have a place in these studies, by far the best models are those that rely on volunteers and provide physiologically more accurate mechanisms of skin pathogenesis and healing.

Sign in / Sign up

Export Citation Format

Share Document