scholarly journals Preparation of Antioxidant Protein Hydrolysates from Pleurotus geesteranus and Their Protective Effects on H2O2 Oxidative Damaged PC12 Cells

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5408
Author(s):  
Xiyu Liao ◽  
Zhenjun Zhu ◽  
Shujian Wu ◽  
Mengfei Chen ◽  
Rui Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pc12 Cells ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Protein Hydrolysates ◽  
Scavenging Activity ◽  
Protective Effects ◽  
Antioxidant Power ◽  
Promising Source

Pleurotus geesteranus is a promising source of bioactive compounds. However, knowledge of the antioxidant behaviors of P. geesteranus protein hydrolysates (PGPHs) is limited. In this study, PGPHs were prepared with papain, alcalase, flavourzyme, pepsin, and pancreatin, respectively. The antioxidant properties and cytoprotective effects against oxidative stress of PGPHs were investigated using different chemical assays and H2O2 damaged PC12 cells, respectively. The results showed that PGPHs exhibited superior antioxidant activity. Especially, hydrolysate generated by alcalase displayed the strongest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (91.62%), 2,2-azino-bis (3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) radical scavenging activity (90.53%), ferric reducing antioxidant power, and metal ion-chelating activity (82.16%). Analysis of amino acid composition revealed that this hydrolysate was rich in hydrophobic, negatively charged, and aromatic amino acids, contributing to its superior antioxidant properties. Additionally, alcalase hydrolysate showed cytoprotective effects on H2O2-induced oxidative stress in PC12 cells via diminishing intracellular reactive oxygen species (ROS) accumulation by stimulating antioxidant enzyme activities. Taken together, alcalase hydrolysate of P. geesteranus protein can be used as beneficial ingredients with antioxidant properties and protective effects against ROS-mediated oxidative stress.

scholarly journals Evaluation of the Antioxidant and Antimicrobial Activities of Porcine Liver Protein Hydrolysates Obtained Using Alcalase, Bromelain, and Papain

2020 ◽  
Vol 10 (7) ◽  
pp. 2290 ◽  
Author(s):  
Paula Borrajo ◽  
Mirian Pateiro ◽  
Mohammed Gagaoua ◽  
Daniel Franco ◽  
Wangang Zhang ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Protein Hydrolysates ◽  
Liver Protein ◽  
Scavenging Activity ◽  
Porcine Liver ◽  
Membrane Pore ◽  
Antioxidant Power

In order to make the by-products generated from the porcine industry more valuable, pig livers were used in this trial to obtain protein hydrolysates. Three proteases (alcalase, bromelain, and papain) were utilized for enzymatic hydrolysis with two different durations, 4 and 8 hours. Ultrafiltration process was used for the recovery of the extracts, employing three different membrane pore sizes (30, 10, and 5 kDa). The porcine livers contained considerable amounts of protein (19.0%), considering they are almost composed of water (74.1%). The antioxidant activity of the obtained hydrolysates was investigated using four antioxidant methods (2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2-2′-Azino-di-[3-ethylbenzthiazoline sulfonate] (ABTS) radical scavenging activity, ferric reducing antioxidant power assay (FRAP), and oxygen radical absorbance capacity assay (ORAC)). Antibacterial properties were also measured against Gram-negative and Gram-positive bacteria. Results indicated that the three studied factors (type of enzyme, membrane pore size, and time) significantly affected the parameters evaluated. Hydrolysates obtained at 8 hours with alcalase had the best antioxidant properties. The 30 kDa alcalase extracts exhibited the highest DPPH (562 µg Trolox/g), FRAP (82.9 µmol Fe2+/100 g), and ORAC (53.2 mg Trolox/g) activities, while for ABTS the 10 kDa alcalase showed the higher values (1068 mg ascorbic acid/100 g). Concerning the antibacterial activity, 30 kDa hydrolysates obtained with bromelain for 4 hours exhibited the highest antimicrobial capacity, providing an inhibition of 91.7%.

scholarly journals Protective Effects of the Hydroethanolic Extract of Fridericia chica on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL)

Toxins ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 748
Author(s):  
Neda Alvarez-Ortega ◽  
Karina Caballero-Gallardo ◽  
María Taboada-Alquerque ◽  
Jackeline Franco ◽  
Elena E. Stashenko ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Neuroblastoma Cells ◽  
Scavenging Activity ◽  
Protective Effects ◽  
Human Neuroblastoma ◽  
Dependent Relationship ◽  
Dpph Method ◽  
Hydroethanolic Extract

Fridericia chica (Bignoniaceae) is a traditional medicinal plant. The aim of this research was to determine the protective effects of the hydroethanolic extract from the F. chica leaves (HEFc) against the cytotoxicity of zearalenone (α-ZEL) and β-ZEL on SH-SY5Y cells. Free radical scavenging activity of HEFc was evaluated using the DPPH method. The cytotoxicity of both zearalenone metabolites and HEFc was examined using MTT test, as was the cytoprotective effects of the HEFc on cells treated with these mycotoxins. The chemical composition of HEFc was determined using UPLC-QTOF-MS/MS. HEFc elicited good DPPH radical scavenging activity following a concentration-dependent relationship. Cells exposed to α-ZEL exhibited a viability ˂50% after 48 h of treatment (25 and 50 µM), while those exposed to β-ZEL showed viability ˂50% (100 µM) and ˂25% (25-100 µM) after 24 and 48 h of exposure, respectively. HEFc showed a significant increase in cell viability after exposure to α-ZEL (25 and 50 µM) and β-ZEL (6–100 µM) (p < 0.05). UPLC-QTOF-MS/MS analyses allowed the identification of 10 phytochemical components in the HEFc. In short, the hydroethanolic extract of F. chica grown in Colombian Caribbean can protect against the effects of mycotoxins and it is a valuable source of compounds with antioxidant properties.

scholarly journals Antioxidant Properties of Popular Turmeric(Curcuma longa)Varieties from Bangladesh

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
E. M. Tanvir ◽  
Md. Sakib Hossen ◽  
Md. Fuad Hossain ◽  
Rizwana Afroz ◽  
Siew Hua Gan ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Curcuma Longa ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Scavenging Activity ◽  
Free Radical Damage ◽  
Antioxidant Power

We investigated the aqueous and ethanolic extracts of different forms (local names:muraandchora) of turmeric(Curcuma longa)from the Khulna and Chittagong divisions of Bangladesh for their antioxidant properties and polyphenol, flavonoid, tannin, and ascorbic acid contents. The antioxidant activity was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging activity and ferric reducing antioxidant power (FRAP) values. The ethanolic extract of Chittagong’s mura contained the highest concentrations of polyphenols (16.07%), flavonoids (9.66%), and ascorbic acid (0.09 mg/100 g) and chora resulted in high yields (17.39%). The ethanolic extract of Khulna’s mura showed a higher DPPH radical-scavenging activity with the lowest 50% inhibitory concentration (IC50) (1.08 μg/mL), while Khulna’s chora had the highest FRAP value (4204.46±74.48 μM Fe[II]per 100 g). Overall, the ethanolic extract had higher antioxidant properties than those in the aqueous extract. However, the tannin concentration was lower in the ethanolic extract. We conclude that the turmeric varieties investigated in this study are useful sources of natural antioxidants, which confer significant protection against free radical damage.

scholarly journals FREE RADICAL SCAVENGING ACTIVITY OF MARINE SPONGES COLLECTED FROM KOVALAM, CHENNAI

2017 ◽  
Vol 10 (6) ◽  
pp. 321 ◽  
Author(s):  
Yuvarani Thambidurai ◽  
Sudarsanam D ◽  
Habeeb Skm ◽  
Ashok Kumar T
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Marine Sponges ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Frap Assay ◽  
Antioxidant Power

Objective: The main focus of this study is to screen the marine sponges for potent free radical scavenging activity. Methods: Various methods such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assay are employed to ascertain the antioxidant properties of marine sponges namely Dysidea herbacea and Sigmadocia pumila. Results: On analyzing, the result of ABTS assay D. herbacea and S. pumila exhibited almost equal antioxidant properties. While calculating the inhibitory concentration 50% value for DPPH assay, the Sample 1 and 2 has an IC of 655.49 and 826.739 μl, respectively, and in FRAP assay, the Sample 1 and 2 has an IC of 67.587 and 74.57 μg, respectively. Conclusion: Overall from this assay, D. herbacea revealed slightly better antioxidant activity when compared to S. pumila, also which in future may serve as a better source to fight against various diseases

scholarly journals Evaluation of Antidiabetic and Antioxidant Properties ofBrucea javanicaSeed

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Abdulwali Ablat ◽  
Jamaludin Mohamad ◽  
Khalijah Awang ◽  
Jamil A. Shilpi ◽  
Aditya Arya
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Oral Glucose ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Nitric Oxide Radical

The ethanol extract ofB. javanicaseed was fractionated with solvents of different polarities and tested for antioxidant activities by several assays including DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP), ferrous ion chelating activity (FCA), and nitric oxide radical scavenging activity (NORSA) along with their polyphenolic contents. Antidiabetic activity was evaluated both in vitro and in vivo using a glycogen phosphorylaseα(GPα) inhibition assay and oral glucose tolerance test (OGTT) in nondiabetic rats. The ethyl acetate fraction (EAF), rich in tannin, exhibited the strongest antioxidant activities to DPPH, FRAP, and NORSA, except for FCA. The EAF also exerted a dose-depended inhibition of GPα(IC50= 0.75 mg/ml). Further evaluation of hypoglycemic effect on OGGT indicated that rats treated with EAF (125 mg/kg bw) showed a 39.91% decrease (P < 0.05) in blood glucose levels at 30 min, and continuous fall (P < 0.05) of 28.89% and 20.29% was observed in the following hours (60 and 90 min) compared to the normal control during OGTT. The EAF was applied to polyamide column chromatography, and the resulting tannin-free fraction was tested for both GPαinhibition and antioxidant (DPPH only) activity. The GPαinhibitory activity was retained, while antioxidant activity was lost (4.6-fold) after tannin removal. These results concluded that the GPαinhibitory activity initially detected was primarily due to the compounds other than tannins, whereas antioxidant activity was mainly due to the tannins.

scholarly journals Dietary Supplementation of Calendula officinalis Counteracts the Oxidative Stress and Liver Damage Resulted from Aflatoxin

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Mohamed A. Hamzawy ◽  
Ezzeldein S. M. El-Denshary ◽  
Nabila S. Hassan ◽  
Fathia A. Mannaa ◽  
Mosaad A. Abdel-Wahhab
Keyword(s):  
Oxidative Stress ◽  
Phenolic Compounds ◽  
Inflammatory Cytokines ◽  
Liver Damage ◽  
Biochemical Parameters ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Calendula Officinalis

This study was conducted to evaluate the total phenolic compounds, the antioxidant properties, and the hepatorenoprotective potential of Calendula officinalis extract against aflatoxins (AFs-) induced liver damage. Six groups of male Sprague-Dawley rats were treated for 6 weeks included the control; the group fed AFs-contaminated diet (2.5 mg/kg diet); the groups treated orally with Calendula extract at low (CA1) and high (CA2) doses (500 and 1000 mg/kg b.w); the groups treated orally with CA1 and CA2 one week before and during AFs treatment for other five weeks. The results showed that the ethanol extract contained higher phenolic compounds and posses higher 1,1-diphenyl 1-2-picryl hydrazyl (DPPH) radical scavenging activity than the aqueous extract. Animals fed AFs-contaminated diet showed significant disturbances in serum biochemical parameters, inflammatory cytokines, and the histological and histochemical pictures of the liver accompanied by a significant increase in malondialdehyde (MDA) and a significant decrease in superoxide dismutase (SOD) and glutathione peroxidase (GPx) in liver. Calendula extract succeeded to improve the biochemical parameters, inflammatory cytokines, decreased the oxidative stress, and improved the histological pictures in the liver of rats fed AFs-contaminated diet in a dose-dependent manner. It could be concluded that Calendula extract has potential hepatoprotective effects against AFs due to its antioxidant properties and radical scavenging activity.

scholarly journals Phytochemical Contents and Antioxidant Activity of Medicinal Plants from the Rubiaceae Family in Thailand

2021 ◽  
Vol 8 (1) ◽  
pp. 24-31
Author(s):  
Rungcharn Suksungworn ◽  
Sutsawat Duangsrisai
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Nitric Oxide Radical ◽  
Promising Source ◽  
Phytochemical Contents

Several plants of the Rubiaceae family possess potential pharmacological properties, such as antioxidant activity, for subsequent drug development. We investigated the methanolic extracts from the bark and wood of five Rubiaceae species for phenolic and flavonoid contents and antioxidant activity. Regarding the phytochemical contents and antioxidant activity, Mitragyna diversifolia wood (437.57 ± 9.90 mg GAE g-1) and Haldina cordifolia wood (30.11 ± 0.20 mg QE g-1) displayed the highest total phenolic content (TPC) and total flavonoid content (TFC) respectively. Morinda coreia bark followed the highest antioxidant activities (IC50 = 360.58 ± 19.28 µg ml-1) in the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), Catunaregam tomentosa bark (IC50 = 13.96 ± 5.32 µg ml-1) in the nitric oxide radical scavenging activity (NO), M. coreia wood (IC50 = 918.27 ± 0.16 µg ml-1) in the superoxide radical scavenging activity (SO) and M. coreia wood (IC50 = 236.65 ± 1.66 µg ml-1) in ferric reducing antioxidant power activity (FRAP). The TPC and TFC displayed strong correlations with DPPH in M. diversifolia wood and with FRAP in M. diversifolia bark and wood. We found high correlation between TFC and FRAP in all plant extracts except C. tomentosa wood, while no relation was detected between TFC and NO in all plant extracts. Comparing Rubiaceae species, the highest antioxidant potential were showed in C. tomentosa bark. Overall, it is worth mentioning that the Rubiaceae species exhibit potential as a promising source of natural antioxidants.

scholarly journals Protective Effects of Oenothera biennis against Hydrogen Peroxide-Induced Oxidative Stress and Cell Death in Skin Keratinocytes

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 255
Author(s):  
Seung Young Lee ◽  
Chul Hwan Kim ◽  
Buyng Su Hwang ◽  
Kyung-Min Choi ◽  
In-Jun Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Hacat Cells ◽  
Scavenging Activity ◽  
Protective Effects ◽  
Oenothera Biennis ◽  
Skin Keratinocytes

Background: Oenothera biennis (evening primrose) produces bioactive substances with a diverse range of pharmacological functions. However, it is currently unknown whether extract prepared from the aerial parts of O. biennis (APOB) can protect the skin against oxidative stress. Objective: The aim of this study is to investigate the protective effects of APOB against oxidative stress-induced damage in human skin keratinocytes (HaCaT) and elucidate the underlying mechanisms. Methods: We pretreated HaCaT cells with various concentrations of APOB or the antioxidant N-acetyl-L-cysteine before applying H2O2. We then compared the cell viability, intracellular reactive oxygen species (ROS) production, and DNA and mitochondrial damage between pretreated and untreated control cells using a range of assays, flow cytometry, and Western blot analysis and also examined the reducing power and DPPH free radical scavenging activity of APOB. Results: APOB pretreatment significantly increased cell viability, effectively attenuated H2O2-induced comet tail formation, and inhibited H2O2-induced phosphorylation of the histone γH2AX, as well as the number of apoptotic bodies and Annexin V-positive cells. APOB was found to have high reducing power and DPPH radical scavenging activity and also exhibited scavenging activity against intracellular ROS accumulation and restored the loss of mitochondrial membrane potential caused by H2O2. APOB pretreatment almost totally reversed the enhanced cleavage of caspase-3, the degradation of poly (ADP-ribose)-polymerase (PARP), DNA fragmentation that usually occurs in the presence of H2O2, and increased the levels of heme oxygenase-1 (HO-1), a potent antioxidant enzyme that is associated with the induction of nuclear factor-erythroid 2-related factor 2 (Nrf2). Conclusions: APOB can protect HaCaT cells from H2O2-induced DNA damage and cell death by blocking cellular damage related to oxidative stress via a mechanism that affects ROS elimination and by activating the Nrf2/HO-1 signaling pathway.

scholarly journals Protective Effects of Oenothera Biennis Against Hydrogen Peroxide-Induced Oxidative Stress and Cell Death in Skin Keratinocytes

2020 ◽  
Author(s):  
Seung Young Lee ◽  
Chul Hwan Kim ◽  
Buyng Su Hwang ◽  
Kyung-Min Choi ◽  
In-Jun Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Hacat Cells ◽  
Scavenging Activity ◽  
Protective Effects ◽  
Oenothera Biennis ◽  
Skin Keratinocytes

Background: Oenothera biennis (evening primrose) produces bioactive substances with a diverse range of pharmacological functions. However, it is currently unknown whether extract prepared from the aerial parts of O. biennis (APOB) can protect the skin against oxidative stress. To investigate the protective effects of APOB against oxidative stress-induced damage in human skin keratinocytes (HaCaT) and elucidate the underlying mechanisms. Methods: We pretreated HaCaT cells with various concentrations of APOB or the antioxidant N-acetyl-L-cysteine before applying H2O2. We then compared the cell viability, intracellular reactive oxygen species (ROS) production, and DNA and mitochondrial damage between pretreated and untreated control cells using a range of assays, flow cytometry, and Western blot analysis and also examined the reducing power and DPPH free radical-scavenging activity of APOB. Results: APOB pretreatment significantly increased cell viability, effectively attenuated H2O2-induced comet tail formation, and inhibited H2O2-induced phosphorylation of the histone &gamma;H2AX, as well as the number of apoptotic bodies and Annexin V-positive cells. APOB was found to have a high reducing power and DPPH radical-scavenging activity and also exhibited scavenging activity against intracellular ROS accumulation and restored the loss of mitochondrial membrane potential caused by H2O2. APOB pretreatment almost totally reversed the enhanced cleavage of caspase-3, the degradation of poly (ADP-ribose)-polymerase (PARP), DNA fragmentation that usually occurs in the presence of H2O2 and increased the levels of heme oxygenase-1 (HO-1), a potent antioxidant enzyme that is associated with the induction of nuclear factor-erythroid 2-related factor 2 (Nrf2). Conclusions: APOB can protect HaCaT cells from H2O2-induced DNA damage and cell death by blocking cellular damage related to oxidative stress via a mechanism that affects ROS elimination and by activating the Nrf2/HO-1 signaling pathway.

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