scholarly journals Autologous Materials in Regenerative Dentistry: Harvested Bone, Platelet Concentrates and Dentin Derivates

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5330
Author(s):  
Sara Bernardi ◽  
Guido Macchiarelli ◽  
Serena Bianchi
Keyword(s):  
Regenerative Medicine ◽  
Bone Tissue ◽  
Clinical Applications ◽  
Physiological Characteristics ◽  
Sinus Lift ◽  
Platelet Concentrates ◽  
Specific Group ◽  
Ridge Augmentation ◽  
Regenerative Dentistry

The jawbone is a peculiar type of bone tissue, unique for its histological, anatomical and physiological characteristics. Therefore, a defect in the maxilla or in the mandible, because of pathological sequelae is difficult to prevent and to restore. Several biomaterials have been and are currently being developed to respond to the demands of regenerative medicine. A specific group of biomaterials used in regenerative dentistry is represented by the autologous materials. Platelet concentrates harvested bone and dentin derivates are indeed used in an attempt to minimise the alveolar resorption or in vertical ridge augmentation procedures or in sinus lift interventions. The aim of this review is to examine the properties of the above-listed materials, to compare them and to indicate eventual clinical applications.

scholarly journals Therapeutic Effects of the Addition of Fibroblast Growth Factor-2 to Biodegradable Gelatin/Magnesium-Doped Calcium Silicate Hybrid 3D-Printed Scaffold with Enhanced Osteogenic Capabilities for Critical Bone Defect Restoration

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 712
Author(s):  
Wei-Yun Lai ◽  
Yen-Jen Chen ◽  
Alvin Kai-Xing Lee ◽  
Yen-Hong Lin ◽  
Yu-Wei Liu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Calcium Silicate ◽  
Clinical Applications ◽  
Fibroblast Growth Factor 2 ◽  
Therapeutic Effects ◽  
Fibroblast Growth ◽  
3D Printed

Worldwide, the number of bone fractures due to traumatic and accidental injuries is increasing exponentially. In fact, repairing critical large bone defects remains challenging due to a high risk of delayed union or even nonunion. Among the many bioceramics available for clinical use, calcium silicate-based (CS) bioceramics have gained popularity due to their good bioactivity and ability to stimulate cell behavior. In order to improve the shortcomings of 3D-printed ceramic scaffolds, which do not easily carry growth factors and do not provide good tissue regeneration effects, the aim of this study was to use a gelatin-coated 3D-printed magnesium-doped calcium silicate (MgCS) scaffold with genipin cross-linking for regulating degradation, improving mechanical properties, and enhancing osteogenesis behavior. In addition, we consider the effects of fibroblast growth factor-2 (FGF-2) loaded into an MgCS scaffold with and without gelatin coating. Furthermore, we cultured the human Wharton jelly-derived mesenchymal stem cells (WJMSC) on the scaffolds and observed the biocompatibility, alkaline phosphatase activity, and osteogenic-related markers. Finally, the in vivo performance was assessed using micro-CT and histological data that revealed that the hybrid bioscaffolds were able to further achieve more effective bone tissue regeneration than has been the case in the past. The above results demonstrated that this type of processing had great potential for future clinical applications and studies and can be used as a potential alternative for future bone tissue engineering research, as well as having good potential for clinical applications.

scholarly journals Regenerative medicine: characterization of human bone matrix gelatin (BMG) and folded platelet-rich fibrin (F-PRF) membranes alone and in combination (sticky bone)

2021 ◽  
Author(s):  
Lajos Csönge ◽  
Ágnes Bozsik ◽  
Zoltán Tóth-Bagi ◽  
Róbert Gyuris ◽  
János Kónya
Keyword(s):  
Regenerative Medicine ◽  
Blood Cell ◽  
Bone Matrix ◽  
Cell Content ◽  
Membrane Bone ◽  
Local Bone ◽  
Freeze Dried ◽  
Fibrin Network ◽  
Regenerative Dentistry ◽  
Particle Ratio

AbstractDuring the last two decades autologous platelet and leukocyte rich products (PRP; PRF), opened new perspectives in regenerative medicine. In particular regenerative dentistry played a pioneer role in the application of these products in bone regenerative cases. Many aspects of cytokines, such as, growth factor release, blood cell content and its characterization were reported, but some practical questions are still unanswered in the preparation of PRF membranes and sticky bones. A new folding technique was introduced that created a good quality, pliable, and strong F-PRF membrane with a dense fibrin network and more homogenous blood cell distribution. F-PRF produced a very promising sticky bone combined with human freeze-dried cortical bone matrix gelatin (BMG). There hasn’t been much focus on the quality and character of the applied bone and the optimal membrane/bone particle ratio has not been reported. A 0.125 g BMG/ml plasma (1 g/8 ml) seems like the ideal combination with maximal BMG adhesion capacity of the membrane. Particle distribution of BMG showed that 3/4 of the particles ranged between 300–1000 µ, the remnant 1/4 was smaller than 300 µ. The whole F-PRF membrane and its parts were compared with conventional A-PRF membrane concerning their resistance against proteolytic digestion. The F-PRF was superior to A-PRF, which dissolved within 4–5 days, while F-PRF was destroyed only after 11 days, so this provides a better chance for local bone morphogenesis. The F-PRF pieces had similar resistance to the whole intact one, so they can be ideal for surgical procedures without risk of fast disintegration.

scholarly journals Stem Cells and Regenerative Medicine: Myth or Reality of the 21th Century

2015 ◽  
Vol 2015 ◽  
pp. 1-19 ◽  
Author(s):  
J.-F. Stoltz ◽  
N. de Isla ◽  
Y. P. Li ◽  
D. Bensoussan ◽  
L. Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Regenerative Medicine ◽  
Embryonic Stem ◽  
Clinical Applications ◽  
Cardiac Insufficiency ◽  
Immunomodulatory Activity ◽  
Hematopoietic Stem ◽  
Fetal Stem Cells ◽  
Organ Regeneration

Since the 1960s and the therapeutic use of hematopoietic stem cells of bone marrow origin, there has been an increasing interest in the study of undifferentiated progenitors that have the ability to proliferate and differentiate into various tissues. Stem cells (SC) with different potency can be isolated and characterised. Despite the promise of embryonic stem cells, in many cases, adult or even fetal stem cells provide a more interesting approach for clinical applications. It is undeniable that mesenchymal stem cells (MSC) from bone marrow, adipose tissue, or Wharton’s Jelly are of potential interest for clinical applications in regenerative medicine because they are easily available without ethical problems for their uses. During the last 10 years, these multipotent cells have generated considerable interest and have particularly been shown to escape to allogeneic immune response and be capable of immunomodulatory activity. These properties may be of a great interest for regenerative medicine. Different clinical applications are under study (cardiac insufficiency, atherosclerosis, stroke, bone and cartilage deterioration, diabetes, urology, liver, ophthalmology, and organ’s reconstruction). This review focuses mainly on tissue and organ regeneration using SC and in particular MSC.

Growth factor release from platelet concentrates: analytic quantification and characterization for clinical applications

Vox Sanguinis ◽  
2013 ◽  
Vol 105 (2) ◽  
pp. 129-136 ◽  
Author(s):  
C. Durante ◽  
F. Agostini ◽  
L. Abbruzzese ◽  
R. T. Toffola ◽  
S. Zanolin ◽  
...  
Keyword(s):  
Growth Factor ◽  
Clinical Applications ◽  
Platelet Concentrates ◽  
Growth Factor Release ◽  
Factor Release

Electrical impedance spectroscopy – a potential method for the study and monitoring of a bone critical-size defect healing process treated with bone tissue engineering and regenerative medicine approaches

2016 ◽  
Vol 4 (16) ◽  
pp. 2757-2767 ◽  
Author(s):  
Evgeny Kozhevnikov ◽  
Xiaolu Hou ◽  
Shupei Qiao ◽  
Yufang Zhao ◽  
Chunfeng Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Critical Size ◽  
Healing Process ◽  
Potential Method ◽  
Electrical Impedance Spectroscopy ◽  
Critical Size Defect ◽  
Critical Size Defects

The development of strategies of bone tissue engineering and regenerative medicine has been drawing considerable attention to treat bone critical-size defects (CSDs).

scholarly journals PLATELET CONCENTRATES- PREPARATION PROTOCOLS AND RECENT ADVANCES

2020 ◽  
pp. 85-87
Author(s):  
Prathamesh Fulsundar ◽  
Vijaysinh More
Keyword(s):  
Tissue Repair ◽  
Autologous Blood ◽  
Effective Means ◽  
Cost Effective ◽  
Blood Platelet ◽  
Clinical Applications ◽  
Prolonged Release ◽  
Platelet Concentrates ◽  
Platelet Rich Fibrin ◽  
Tissue Healing

Autologous blood derivatives are surgical biologic additive that are prepared by manipulation of autologous blood. Platelet rich fibrin is one of the most commonly used blood derivatives in dentistry. Blood derivatives have several advantages such as being 100% autogenous, cost effective, less time consuming, simple to perform and with superior & prolonged release of growth factors. Since inception there has been evolution of various techniques, in-depth research regarding its biological actions, clinical applications. Several modifications have been advocated in the conventional protocol like the advanced PRF, injectable PRF, PRF lysate and Titanium-prepared PRF. Hence, the aim of this article is to review various types and properties of blood derivatives and the advancement in the PRF technology since its inception. Furthermore, platelet concentrates are safe, reliable and cost-effective means to accelerate tissue healing and for improving the efficiency of tissue repair after injury.

Bone tissue regenerative medicine via bioactive nanomaterials

2017 ◽  
pp. 769-792 ◽  
Author(s):  
Paula V. Messina ◽  
Noelia L. D’Elía ◽  
Luciano A. Benedini
Keyword(s):  
Regenerative Medicine ◽  
Bone Tissue

Regenerative medicine: clinical applications of stem cells

Stem Cells ◽  
2021 ◽  
pp. 159-220
Author(s):  
Christine L. Mummery ◽  
Anja van de Stolpe ◽  
Bernard Roelen ◽  
Hans Clevers
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Clinical Applications
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