scholarly journals Cicer arietinum L. Sprouts’ Influence on Mineralization of Saos-2 and Migration of MCF-7 Cells

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4490
Author(s):  
Małgorzata Zakłos-Szyda ◽  
Ilona Gałązka-Czarnecka ◽  
Joanna Grzelczyk ◽  
Grażyna Budryn
Keyword(s):  
Biological Activity ◽  
Cicer Arietinum ◽  
Biochanin A ◽  
Cicer Arietinum L ◽  
Prevention Of Osteoporosis ◽  
And Migration ◽  
Induced Apoptosis ◽  
Factor Α ◽  
Necrosis Factor ◽  
Mcf 7

In the present study, we investigated the biological activity of four extracts obtained from Cicer arietinum L. sprouts. The fermentation of the sprouts with Lactobacillus casei and their incubation with β-glucosidase elevated the concentrations of isoflavonoids, especially coumestrol, formononetin and biochanin A. To study the biological activity of C. arietinum, the human osteosarcoma Saos-2 and human breast cancer MCF-7 cell lines were used. The extracts obtained from fermented sprouts exhibited the strongest ability to decrease intracellular oxidative stress in both types of cells. They augmented mineralization and alkaline phosphatase activity in Saos-2 cells, as well as diminished the secretion of interleukin-6 and tumor necrosis factor α. Simultaneously, the extracts, at the same doses, inhibited the migration of MCF-7 cells. On the other hand, elevated concentrations of C. arietinum induced apoptosis in estrogen-dependent MCF-7 cells, while lower doses stimulated cell proliferation. These results are important for carefully considering the use of fermented C. arietinum sprouts as a dietary supplement component for the prevention of osteoporosis.

scholarly journals Estradiol Abrogates Apoptosis in Breast Cancer Cells through Inactivation of BAD: Ras-dependent Nongenomic Pathways Requiring Signaling through ERK and Akt

2004 ◽  
Vol 15 (7) ◽  
pp. 3266-3284 ◽  
Author(s):  
Romaine Ingrid Fernando ◽  
Jay Wimalasena
Keyword(s):  
Breast Cancer ◽  
Tumor Necrosis Factor ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Dominant Negative ◽  
Induced Apoptosis ◽  
Factor Α ◽  
Necrosis Factor

Estrogens such as 17-β estradiol (E2) play a critical role in sporadic breast cancer progression and decrease apoptosis in breast cancer cells. Our studies using estrogen receptor-positive MCF7 cells show that E2 abrogates apoptosis possibly through phosphorylation/inactivation of the proapoptotic protein BAD, which was rapidly phosphorylated at S112 and S136. Inhibition of BAD protein expression with specific antisense oligonucleotides reduced the effectiveness of tumor necrosis factor-α, H2O2, and serum starvation in causing apoptosis. Furthermore, the ability of E2 to prevent tumor necrosis factor-α-induced apoptosis was blocked by overexpression of the BAD S112A/S136A mutant but not the wild-type BAD. BAD S112A/S136A, which lacks phosphorylation sites for p90RSK1 and Akt, was not phosphorylated in response to E2 in vitro. E2 treatment rapidly activated phosphatidylinositol 3-kinase (PI-3K)/Akt and p90RSK1 to an extent similar to insulin-like growth factor-1 treatment. In agreement with p90RSK1 activation, E2 also rapidly activated extracellular signal-regulated kinase, and this activity was down-regulated by chemical and biological inhibition of PI-3K suggestive of cross talk between signaling pathways responding to E2. Dominant negative Ras blocked E2-induced BAD phosphorylation and the Raf-activator RasV12T35S induced BAD phosphorylation as well as enhanced E2-induced phosphorylation at S112. Chemical inhibition of PI-3K and mitogen-activated protein kinase kinase 1 inhibited E2-induced BAD phosphorylation at S112 and S136 and expression of dominant negative Ras-induced apoptosis in proliferating cells. Together, these data demonstrate a new nongenomic mechanism by which E2 prevents apoptosis.

scholarly journals Stoffwechsel aromatischer Pflanzeninhaltsstoffe I

1969 ◽  
Vol 24 (2) ◽  
pp. 234-239 ◽  
Author(s):  
Wolfgang Barz
Keyword(s):  
Cicer Arietinum ◽  
Half Life ◽  
Biochanin A ◽  
Pulse Labelling ◽  
Phaseolus Aureus ◽  
Cicer Arietinum L ◽  
Plant Products ◽  
Biological Half Life ◽  
Total Turnover ◽  
Secondary Plant

The turnover of the isoflavones, formononetin and biochanin A, in Cicer arietinum L. and of the isoflavone, daidzein, and the coumestan, coumestrol, in Phaseolus aureus Roxb. has been determined by pulse labelling. The biological half-life of formononetin, daidzein and coumestrol has been found to be approximately 50 hours, while the turnover of biochanin A appeared to be very slow. The excretion of all four plant products through the roots into the medium has been measured. Approximately 3 percent of the total turnover of isoflavones in the plants can be accounted for by excretion into the medium.The results are discussed in view of the concept that secondary plant products may well be subject to further metabolism.

Zur Biogenese der Isoflavone

1961 ◽  
Vol 16 (1) ◽  
pp. 2-5 ◽  
Author(s):  
Hans Grisebach ◽  
Gerhard Brandner
Keyword(s):  
Cicer Arietinum ◽  
Cinnamic Acid ◽  
Red Clover ◽  
Biochanin A ◽  
Cicer Arietinum L ◽  
The One

With the aid of cinnamic acid-[3-14C] and DL-Phenylalanine-[1-14C] it has been demonstrated that the biogenesis of both biochanin-A (5.7-Dihydroxy-4′-methoxyisoflavone) and formononetin (7-Hydroxy-4′-methoxyisoflavone) in chana germ (Cicer arietinum L.) involves a phenyl migration like the one which has been found to occur in the biogenesis of formononetin in red clover. 4.4′.6′-Trihydroxychalcone-4′-glucoside-[β-14C] is incorporated into formononetin but not into biochanin-A, the entire radioactivity being localized in C-2 of formononetin. Thus it has been proven that a C6-C3-C6 compound can be converted into an isoflavone. Acetate-[1-14C] is incorporated almost exclusively into ring A of formononetin.

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