scholarly journals Identification and Characterization of α-Glucosidase Inhibition Flavonol Glycosides from Jack Bean (Canavalia ensiformis (L.) DC

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2481
Author(s):  
Anita M. Sutedja ◽  
Emiko Yanase ◽  
Irmanida Batubara ◽  
Dedi Fardiaz ◽  
Hanifah N. Lioe
Keyword(s):  
2D Nmr ◽  
The Other ◽  
Inhibition Activity ◽  
Jack Bean ◽  
Canavalia Ensiformis ◽  
Kaempferol Glycosides ◽  
Uv Visible ◽  
First Time ◽  
Identification And Characterization

Although the intake of jack bean (Canavalia ensiformis (L.) DC.), an underutilized tropical legume, can potentially decrease the risk of several chronic diseases, not much effort has been directed at profiling the polyphenolics contained therein. Hence, this work aimed to identify and quantify the dominant jack bean polyphenolics, which are believed to have antioxidant and other bioactivities. Four major compounds were detected and identified as kaempferol glycosides with three or four glycoside units. Their structures were established based on UV-visible, 1D, 2D NMR, and HR-ESI-MS analyses. Specifically, kaempferol 3-O-α-l-rhamnopyranosyl (1→6)- β-d-glucopyranosyl (1→2)-β-d-galactopyranosyl-7-O-[3-O-o-anisoyl]-α-l-rhamnopyranoside was detected for the first time, while the other three compounds have already been described in plants other than jack bean. This new compound was found to have a higher α-glucosidase inhibition activity compared to acarbose.

scholarly journals Isolation and Characterization of Crotosparsamide, a New Cyclic Nonapeptide from Croton sparsiflorus

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Rashad Mehmood ◽  
Abdul Malik
Keyword(s):  
Spectroscopic Data ◽  
2D Nmr ◽  
Spectral Studies ◽  
Isolation And Characterization ◽  
First Time

Crotosparsamide (1), a new cyclic nonapeptide, has been isolated from the n-butanol soluble sub-fraction of Croton sparsiflorus along with p-hydroxy methylcinnamate and kaempferol, which are reported for the first time from this species. Their structures were determined by chemical and spectral studies including ESIMS, and 1D and 2D NMR spectroscopic data.

Dispersal of linezolid-resistant enterococci carrying poxtA or optrA in retail meat and food-producing animals from Tunisia

2019 ◽  
Vol 74 (10) ◽  
pp. 2865-2869 ◽  
Author(s):  
Houyem Elghaieb ◽  
Ana R Freitas ◽  
Mohamed Salah Abbassi ◽  
Carla Novais ◽  
Mohamed Zouari ◽  
...  
Keyword(s):  
Developed Countries ◽  
Cow Milk ◽  
Urban Wastewater ◽  
Virulence Markers ◽  
Linezolid Resistance ◽  
Alternative Measures ◽  
Retail Meat ◽  
First Time ◽  
Identification And Characterization

Abstract Objectives The epidemiology of Enterococcus resistant to priority antibiotics including linezolid has mainly been investigated in developed countries and especially in hospitals. We aimed to evaluate the contribution of different non-human reservoirs for the burden of MDR enterococci in Tunisia, where scarce data are available. Methods Samples (n = 287) were collected from urban wastewater (n = 57), retail meat (n = 29; poultry/bovine/ovine), milk (n = 89; bovine/ovine), farm animal faeces (n = 80; poultry/bovine/ovine) and pets (n = 32; rabbit/dogs/cats/birds) in different Tunisian regions (2014–17). They were plated onto Slanetz–Bartley agar after pre-enrichment without antibiotics. Standard methods were used for bacterial identification and characterization of antibiotic resistance and virulence genes (PCR), antibiotic susceptibility testing (disc diffusion/broth microdilution; EUCAST/CLSI) and clonality (SmaI-PFGE/MLST). Results All samples carried Enterococcus (n = 377 isolates) resistant to antibiotics considered to be critical or highly important by WHO. Even without antibiotic selection, 38% of Enterococcus faecalis (Efs) and 22% of Enterococcus faecium (Efm) were identified as MDR. Linezolid-resistant isolates (5%; MIC = 8 mg/L) comprised six poxtA-carrying Efm (cow milk), seven optrA-carrying Efs (chicken faeces/meat) and five Efm lacking cfr/optrA/poxtA (poultry/bovine/ovine/wastewater). Clinically relevant Efm clones (clade A1) were identified in animal/meat sources. Ampicillin resistance (1%) was confined to ST18/ST78-like MDR Efm clones from bovine meat/milk samples carrying relevant virulence markers (e.g. ptsD/IS16). Conclusions This study provides evidence of the contribution of livestock and foodstuffs to the dispersal of acquired linezolid resistance genes including poxtA and optrA. We report the first poxtA-carrying Efm in Tunisia, and for the first time in bovine samples, stressing the urgent need for alternative measures to counteract the spread of linezolid-resistant enterococci globally.

scholarly journals A small mitochondrial protein present in myzozoans is essential for malaria transmission

Open Biology ◽  
2016 ◽  
Vol 6 (4) ◽  
pp. 160034 ◽  
Author(s):  
Dennis Klug ◽  
Gunnar R. Mair ◽  
Friedrich Frischknecht ◽  
Ross G. Douglas
Keyword(s):  
Drug Targets ◽  
Mitochondrial Protein ◽  
Sister Group ◽  
Developmental Defect ◽  
Mitochondrial Mass ◽  
First Time ◽  
Potential Drug Targets ◽  
Identification And Characterization

Myzozoans (which include dinoflagellates, chromerids and apicomplexans) display notable divergence from their ciliate sister group, including a reduced mitochondrial genome and divergent metabolic processes. The factors contributing to these divergent processes are still poorly understood and could serve as potential drug targets in disease-causing protists. Here, we report the identification and characterization of a small mitochondrial protein from the rodent-infecting apicomplexan parasite Plasmodium berghei that is essential for development in its mosquito host. Parasites lacking the gene mitochondrial protein ookinete developmental defect ( mpodd ) showed malformed parasites that were unable to transmit to mosquitoes. Knockout parasites displayed reduced mitochondrial mass without affecting organelle integrity, indicating no role of the protein in mitochondrial biogenesis or morphology maintenance but a likely role in mitochondrial import or metabolism. Using genetic complementation experiments, we identified a previously unrecognized Plasmodium falciparum homologue that can rescue the mpodd(−) phenotype, thereby showing that the gene is functionally conserved. As far as can be detected, mpodd is found in myzozoans, has homologues in the phylum Apicomplexa and appears to have arisen in free-living dinoflagellates. This suggests that the MPODD protein has a conserved mitochondrial role that is important for myzozoans. While previous studies identified a number of essential proteins which are generally highly conserved evolutionarily, our study identifies, for the first time, a non-canonical protein fulfilling a crucial function in the mitochondrion during parasite transmission.

scholarly journals Photoluminescence of Pentavalent Uranyl Amide Complexes

2021 ◽  
Author(s):  
Fabrizio Ortu ◽  
simon Randall ◽  
David J. Moulding ◽  
Adam woodward ◽  
Karsten Meyer ◽  
...  
Keyword(s):  
Electronic Structure ◽  
Excited State ◽  
Charge Transfer ◽  
Synthesis And Characterization ◽  
New Family ◽  
Theoretical Investigations ◽  
Charge Transfer Transitions ◽  
Uv Visible ◽  
First Time

We report the synthesis and characterization of a new family of pentavalent uranyl amide complexes, supported also by photoluminescence and theoretical investigations. These studies reveal for the first time that the UV-visible emission of uranyl(V) is an admixture of charge transfer transitions accompanied by vibronic coupling of the quartet excited state with uranyl oxo and amide vibrations, thereby offering new insights into the electronic structure of the reactive uranyl(V)

scholarly journals Isolation, Identification and Characterization of Gefitinib Novel Degradation Products by NMR and HRMS, Method Development and Validation by UPLC

2021 ◽  
Vol 33 (8) ◽  
pp. 1743-1748
Author(s):  
Ramulu Yanaka ◽  
Hima Bindu Gandham ◽  
Chidananda Swamy Rumalla ◽  
Muralidharan Kaliyaperumal ◽  
Shaik John Saida ◽  
...  
Keyword(s):  
Method Development ◽  
Degradation Products ◽  
2D Nmr ◽  
The Novel ◽  
Ich Guidelines ◽  
Stress Degradation ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Identification And Characterization

Gefitinib (GFT) sold under the brand name Iressa, is a medication used to treat certain type of breast, lung and other cancers, Gefitinib was subject to stress degradation under acidic, basic, peroxide mediated oxidation, photolytic and thermal degradation. The stress degradation was performed according to ICH guidelines Q1A(R2) and the drug was inert under thermal and photolytic conditions. One degradant is identified in acid hydrolysis referred as 7-methoxy-6-(3-morpholinopropoxy) quinazolin-4(3H)-one (GFT-DP1) and two degradants were formed in peroxide mediated hydrolysis referred as 4-(3-((4-((3- chloro-4-fluorophenyl)amino)-7-methoxy-1-oxidoquinazolin-6-yl)oxy)-propyl)morpholine-4-oxide (GFT-DP2) and 4-(3-((4-((3-chloro-4-fluorophenyl)amino)-7-methoxyquinazolin-6-yl)oxy)-propyl)- morpholine-4-oxide (GFT-DP3). In present study, all the novel three degradation product structures were confirmed by HRMS and 1D (1H, 13C) and 2D (COSY, HSQC and HMBC) based on 1D and 2D NMR data proton and carbon chemical shift values assigned exactly for all degradation products. A stability indicating RP-UPLC method was developed and validated with shorter run time and this method was validated in terms of linearity, specificity, accuracy, LOD and LOQ.

scholarly journals Characterization of the Structure of 2-(4-methyl-2 Phenyl-4, 5-dihydrooxazol-4-ylmethyl)-isoindole-1, 3-dione by 1D, COSY and HSQC 2D NMR Spectroscopy

2021 ◽  
pp. 30-37
Author(s):  
Khadim Dioukhane ◽  
Younas Aouine ◽  
Asmae Nakkabi ◽  
Salaheddine Boukhssas ◽  
Hassane Faraj ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
2D Nmr ◽  
The Other ◽  
Two Dimensional ◽  
Hsqc Spectrum ◽  
2D Nmr Spectroscopy ◽  
Perfect Correlation ◽  
Dione Derivative ◽  
The One

The identity of the 2-(4-Methyl-2-phenyl-4,5-dihydrooxazol-4-ylmethyl)-isoindole-1,3-dione, previously synthesized in our laboratory, was proven without doubt by means of 1D and 2D NMR spectroscopy. Two-dimensional NMR spectroscopy played a major role. The analysis of the 2D-COSY spectrum of isoindoline-1,3-dione derivative shows a perfect correlation between neighboring protons. Thus, a correlation was noted between the protons of the phthalimide, H(8) and H(9) on the one hand and H(8') and H(9') on the other hand. The analysis of the 2D-HSQC spectrum of the studied compound indicates a faultless correlation between protons and adjacent carbons, and no correlation in the case of all quaternary carbons.

scholarly journals SUBORDERS ACOTYLEA AND COTYLEA (POLYCLADIDA): STUDY ON MORPHOLOGICAL, ECOLOGICAL AND REPRODUCTIVE FEATURES OF SOME REPRESENTATIVE SPECIES FROM TUNISIAN COASTS (MEDİTERRANEAN)

2017 ◽  
Vol 10 ◽  
pp. 58-70
Author(s):  
Mehrez Gammoudi ◽  
Saïda Tekaya
Keyword(s):  
Parental Care ◽  
The Other ◽  
Morphological Data ◽  
Associated Fauna ◽  
Other Hand ◽  
Representative Species ◽  
First Time ◽  
Made In

The aim of this work is to provide some important morphological, ecological and reproductive features of 8 polyclad species from Tunisian waters belonging to Acotylea: Echinoplana celerrima Haswell, 1907, Leptoplana mediterranea (Bock, 1913), Discocelis tigrina (Blanchard, 1847) and Imogine mediterranea (Galleni, 1976) and Cotylea: Thysanozoon brocchii (Risso, 1818), Prosthiostomum siphunculus (Delle Chiaje, 1822), Yungia aurantiaca (Delle Chiaje, 1822) and Prostheceraeus moseleyi (Lang, 1884). New data on distribution of some species are added. Moreover, morphological data are provided for the first time in living specimens of D. tigrina. Based on our specimens, we confirm characterization of the two sub-orders Acotylea and Cotylea that have been already made in previous studies. Function of attachment organs in polyclads is discussed. On the other hand, data dealing with associated fauna are offered for all species. The two acotyleans E. celerrima and I. mediterranea were seen to cover their egg plates practicing thereby a parental care. This work could be a baseline for future taxonomic and behavioural investigations.

scholarly journals Identification and characterization of 19 predicted Myb-family DNA binding proteins in Magnaporthe oryzae concerning growth, conidiation, and pathogenicity

2021 ◽  
Author(s):  
Ya Li ◽  
Xiuxia Zheng ◽  
Mengtian Pei ◽  
Mengting Chen ◽  
Shengnan Zhang ◽  
...  
Keyword(s):  
Stress Response ◽  
Dna Binding ◽  
Magnaporthe Oryzae ◽  
Expression Profiles ◽  
Dna Binding Proteins ◽  
The Other ◽  
Genes Encoding ◽  
Identification And Characterization ◽  
Different Levels

Genes encoding for proteins containing the DNA binding Myb domain have been suggested to be important in regulating development and stress response in eukaryotes, including fungi. Magnaporthe oryzae (teleomorph Pyricularia oryzae) is considered the most destructive pathogen of rice. We screen the M. oryzae genome for all genes encoding proteins containing Myb domains since these genes could be essential during pathogenesis. We found 19 genes Myb1-19. Only a few have previously been investigated, and only one has proven to be involved in pathogenesis. We tried to delete the other 18 genes and succeeded with all except 6, five of which could be essential. RT-qPCR showed that all 19 genes are expressed during pathogenesis, although at different levels and with different expression profiles. To our surprise, only deletions of the genes encoding proteins MoMyb2, MoMyb13, and MoMyb15 showed growth, conidiation, and infection phenotypes, indicating that they are essential on their own during infection. This lack of phenotypes for the other mutants surprised us, and we extended the analysis to look for expression co-regulation and found 5 co-regulated groups of predicted proteins with Myb-domains. We point to likely compensatory regulations of the other Myb-family genes hiding the effect of many deletions. Further studies of the Myb-family genes are thus of interest since revealing the functions of these genes with a possible effect on pathogenicity since these could be targets for future measures to control M. oryzae in rice.

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