scholarly journals Grape Cane Extracts as Multifunctional Rejuvenating Cosmetic Ingredient: Evaluation of Sirtuin Activity, Tyrosinase Inhibition and Bioavailability Potential

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2203
Author(s):  
Magdalena Anna Malinowska ◽  
Kévin Billet ◽  
Samantha Drouet ◽  
Thibaut Munsch ◽  
Marianne Unlubayir ◽  
...  
Keyword(s):  
Hplc Analysis ◽  
Major Constituent ◽  
Skin Permeability ◽  
Waste Biomass ◽  
Inhibition Assay ◽  
Tyrosinase Inhibition ◽  
Skin Whitening ◽  
Cosmetic Ingredients ◽  
Grape Varieties ◽  
Activation Capacity

Grape canes are waste biomass of viticulture containing bioactive polyphenols valuable in cosmetics. Whereas several studies reported the cosmetic activities of E-resveratrol, only few described the potential of E-ε-viniferin, the second major constituent of grape cane extracts (GCE), and none of them investigated GCE as a natural blend of polyphenols for cosmetic applications. In this study, we considered the potential of GCE from polyphenol-rich grape varieties as multifunctional cosmetic ingredients. HPLC analysis was performed to quantify major polyphenols in GCE i.e., catechin, epicatechin, E-resveratrol, E-piceatannol, ampelopsin A, E-ε-viniferin, hopeaphenol, isohopeaphenol, E-miyabenol C and E-vitisin B from selected cultivars. Skin whitening potential through tyrosinase inhibition assay and the activation capacity of cell longevity protein (SIRT1) of GCE were compared to pure E-resveratrol and E-ε-viniferin. Drug-likeness of GCE polyphenols were calculated, allowing the prediction of skin permeability and bioavailability. Finally, the present data enabled the consideration of GCE from polyphenol-rich varieties as multifunctional cosmetic ingredients in accordance with green chemistry practices.

scholarly journals Potent Microbial and Tyrosinase Inhibitors from Stem Bark of Bauhinia Rufescens (Fabaceae)

2013 ◽  
Vol 8 (10) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Aminu Muhammad ◽  
Hasnah Mohd Sirat
Keyword(s):  
Kojic Acid ◽  
Stem Bark ◽  
Positive Control ◽  
Inhibition Assay ◽  
Bacterial Strains ◽  
Tyrosinase Inhibition ◽  
Fungal Strains ◽  
Inhibition Concentration ◽  
Tyrosinase Enzyme ◽  
Antimicrobial Assay

The stem bark extracts of Bauhinia rufescens Lam. (Fabaceae) yielded 6-methoxy-7-methyl-8-hydroxydibenz[ b,f]oxepin, α-amyrin acetate, β-sitosterol 3- O-β-D-xylopyranoside, 4-(2′-Hydroxyphenethyl)-5-methoxy-2-methylphenol, menisdaurin and sequoyitol. Their structures were determined using spectroscopic methods and comparisons with the literature data. For the antimicrobial assay Gram-positive and Gram-negative bacterial and fungal strains were tested, while the tyrosinase inhibition assay utilized L-DOPA as a substrate for the tyrosinase enzyme. 6-Methoxy-7-methyl-8-hydroxydibenz[ b,f]oxepin, α-amyrin acetate, β-sitosterol 3- O-β-D-xylopyranoside, menisdaurin and sequoyitol showed weak to moderate activities with minimum inhibition concentration (MIC) values in the range of 112.5–900 μg/mL against all bacterial strains, while the MIC values for the fungal strains were in the range of 28.1–450 μg/mL. In the tyrosinase inhibition assay, α-amyrin acetate was found to be moderately active against tyrosinase with an inhibition of 62% at 0.1 mg/mL. This activity was lower than that of the positive control, kojic acid (85%).

scholarly journals Characterizing Tyrosinase Modulators from the Roots of Angelica keiskei Using Tyrosinase Inhibition Assay and UPLC-MS/MS as the Combinatorial Novel Approach

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3297 ◽  
Author(s):  
Jia-Hao Lee ◽  
Hui-Ching Mei ◽  
I-Chih Kuo ◽  
Tzong-Huei Lee ◽  
Yu-Hsin Chen ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Ultra Performance Liquid Chromatography ◽  
Rapid Screening ◽  
Inhibition Assay ◽  
Tyrosinase Inhibition ◽  
Novel Approach ◽  
Ic50 Values ◽  
Angelica Keiskei ◽  
Screening And Identification

In this study, an in vitro tyrosinase inhibition assay in combination with ultra performance liquid chromatography-orbitrap mass spectrometry (UPLC-orbitrap-MS) was developed for the rapid screening and identification of tyrosinase modulators from roots of Angelica keiskei. Of the 15 candidates considered, nine chalcones, xanthoangelols (1), B (2), D (3), E (4), G (5), H (6), 4-hydroxyderricin (7), xanthokeismin B (8) and (2E)-1-[4-hydroxy-2-(2-hydroxy-2-propanyl)-2,3-dihydro-1-benzofuran-7-yl]-3-(4-hydroxyphenyl)-2-propen-1-one (9), five coumarins, umbelliferone (10), selinidin (11), isopimpinellin (12), phellopterin (13) and xanthyletin (14), and one other compound, ashitabaol A (15), were distinguished between the test samples and the controls with statistical significance, and the structure of each compound was determined by comparing with in-house standards and the literature. Among these, six compounds, xanthoangelol (1), xanthoangelol D (3), xanthoangelol H (6), 4-hydroxyderricin (7), laserpitin (16) and isolaserpitin (17), were isolated from roots of A. keiskei. Of the compounds isolated, compounds 1, 7 and 16 were subjected to tyrosinase inhibitory assay, and the IC50 values were 15.87 ± 1.21, 60.14 ± 2.29 and >100 μM, respectively. The present study indicated that the combination of in vitro tyrosinase inhibition assay coupled with UPLC-MS/MS could be widely applied to the rapid screening of active substances from various natural resources.

scholarly journals In Silico Prediction of Tyrosinase and Adenylyl Cyclase Inhibitors from Natural Compounds

2014 ◽  
Vol 9 (2) ◽  
pp. 1934578X1400900
Author(s):  
Pedro Fong ◽  
Henry H. Y. Tong ◽  
Chi M. Chao
Keyword(s):  
Adenylyl Cyclase ◽  
Skin Irritation ◽  
Herbal Medicines ◽  
Skin Permeability ◽  
Pharmacophore Mapping ◽  
Topical Formulation ◽  
Melanin Production ◽  
Skin Whitening

Although many herbal medicines are effective in the treatment of hyperpigmentation, the potency of different constituents remains unknown. In this work, more than 20,000 herbal ingredients from 453 herbs were docked into the crystal structures of adenylyl cyclase and a human homology tyrosinase model using Surflex-Dock. These two enzymes are responsible for melanin production and inhibition of them may attain a skin-whitening effect superior to currently available agents. The essential drug properties for topical formulation of the herbal ingredients, including skin permeability, sensitization, irritation, corrosive and carcinogenic properties were predicted by Dermwin, Skin Sensitization Alerts (SSA), Skin Irritation Corrosion Rules Estimation Tool (SICRET) and Benigni/Bossa rulebase module of Toxtree. Moreover, similarity ensemble and pharmacophore mapping approaches were used to forecast other potential targets for these herbal compounds by the software, SEArch and PharmMapper. Overall, this study predicted seven compounds to have advanced drug-like properties over the well-known effective tyrosinase inhibitors, arbutin and kojic acid. These seven compounds have the highest potential for further in vitro and in vivo investigation with the aim of developing safe and high-efficacy skin-whitening agents.

scholarly journals Usefulness of Urea as a Means of Improving the Solubility of Poorly Water-Soluble Ascorbyl Palmitate

2017 ◽  
Vol 2017 ◽  
pp. 1-9
Author(s):  
Yutaka Inoue ◽  
Daichi Niiyama ◽  
Isamu Murata ◽  
Ikuo Kanamoto
Keyword(s):  
Radical Scavenging ◽  
Ascorbyl Palmitate ◽  
Water Soluble ◽  
Mushroom Tyrosinase ◽  
Inhibition Assay ◽  
Dpph Radical ◽  
Tyrosinase Inhibition ◽  
Solubility Test ◽  
Crystal System ◽  
Dpph Radical Scavenging

The aim of this study was to evaluate complexes of L-ascorbyl palmitate (ASCP) and urea (UR). This evaluation involved differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD), scanning electron microscopy (SEM), near-infrared spectroscopy (NIR), a solubility test, a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging test, and a mushroom tyrosinase inhibition assay. Physicochemical evaluation revealed that ASCP/UR complexes form at a molar ratio of 1/12. The solubility test revealed that ASCP/UR complexes had increased solubility compared to ASCP. The DPPH radical scavenging test and mushroom tyrosinase inhibition assay revealed that the activity of ASCP/UR complexes was not impaired by complex formation. These results are probably due to the tetragonal crystal system of UR changing to a hexagonal crystal system and interaction with the alkyl group of ASCP.

scholarly journals Effect of Manufacturing and Absorption Promotion of Lidocaine Hydrogel Using Ultrasonic Waves during Cosmetic Procedures

2021 ◽  
Vol 19 (4) ◽  
pp. 619-626
Author(s):  
Sol-Hui Song ◽  
Hoon Kim
Keyword(s):  
Ultrasonic Treatment ◽  
Hplc Analysis ◽  
Skin Penetration ◽  
Ultrasonic Waves ◽  
Viscosity Measurement ◽  
Ultrasound Treatment ◽  
Skin Permeability ◽  
Gelation Rate ◽  
Ultrasound Parameters ◽  
Permeability Experiment

Purpose: In this study, we study to more effectively use anesthesia products used in beauty procedures following the popularization of anti-aging. Hydrogel, which contains lidocaine, is believed to be more effective in relieving pain if used in cosmetic procedures with ultrasonic waves.Methods: The availability of manufactured hydrogels and commercial gels for ultrasonic treatment was compared, and the effect on skin penetration and skin penetration due to ultrasonic limitations was evaluated based on their applicability. Usability and optimal ultrasound parameters were identified during ultrasound treatment.Results: Viscosity measurement, gelation rate, swelling, skin permeability experiment, and HPLC analysis of manufactured hydrogels all revealed properties, with skin permeability being highest at frequency 1 MHz, cycle low 200, and high 50.Conclusions: Finally, hydrogels containing lidocaine increased skin permeability during ultrasound treatment, allowing for faster targeted transdermal transmission that was more effective depending on the ultrasound parameters. As a result, it is determined that it can be used in cosmetic procedures.

Phyto-chemical screening of Root Extracts of Glycyrrhiza glabra by Spectroscopic Methods (UV-VIS Spectrophotometer, FTIR and HPLC)

2019 ◽  
Vol 11 (06) ◽  
Author(s):  
Arti Kumari ◽  
Jainendra Kumar
Keyword(s):  
Hplc Analysis ◽  
Detrimental Effect ◽  
Acne Vulgaris ◽  
Glycyrrhiza Glabra ◽  
Skin Whitening ◽  
Propionibacterium Acne ◽  
Chemical Screening ◽  
Root Extracts ◽  
Bioactive Constituent

Glycyrrhiza glabra, commonly known as licorice, has traditionally been used in various medicinal preparations as expectorant, antimicrobial, antiviral, anticancer, anti allergic agent. It has also prominent role in skin whitening and has anti-inflammatory and esterogenic properties. This study aims to the characterization of the bioactive constituent glabridin in plant extract of Glycyrrhiza glabra in ethanol, methanol and ethyl acetate using UV-VIS and FTIR and HPLC. A wavelength scan (200-600 nm) by UV-Vis spectrophotometer was performed and the characteristic peaks were recorded. The scan confirmed presence of glabridin with λmax at 281 nm with a small number of additional phytochemicals indicated by the extra peaks. FTIR spectrum of 62% pure glabridin was compared to the spectra shown by the three extracts. Presence of glabridin in all the three extracts was corroborated by HPLC analysis with the retention time of 4.05 minute. Glabridin was also evaluated for its antibacterial activity against Propionibacterium acne, causative agent of Acne vulgaris and found to have the detrimental effect at concentration 500, 1000 and 1500 ppm.

scholarly journals Search for Skin-whitening Agent from Prunus Plants and the Molecular Targets in Melanogenesis Pathway of Active Compounds

2014 ◽  
Vol 9 (2) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Kazuya Murata ◽  
Keisuke Takahashi ◽  
Haruka Nakamura ◽  
Kimihisa Itoh ◽  
Hideaki Matsuda
Keyword(s):  
Research Program ◽  
Molecular Targets ◽  
Molecular Target ◽  
The Novel ◽  
Tyrosinase Inhibition ◽  
Prunus Species ◽  
Skin Whitening ◽  
Whitening Agent ◽  
First Time ◽  
Potent Inhibitory Activity

In our research program for discovering novel skin-whitening materials, screening of extracts from flowers of some Prunus species was performed using an anti-tyrosinase assay. Among the tested plants, the flowers of P. persica showed the most potent inhibitory activity. In addition, P. persica also showed suppression of melanogenesis in B16 rat melanoma cells. The active principles of tyrosinase inhibition and suppression of melanogenesis were revealed to be an afzelin (3- O-α-L-rhamnosylkaempferol) and a flavanone, naringenin. The mechanism of the anti-melanogenesis effect of these two compounds was disclosed, for the first time, as the suppression of the expression of tyrosinase protein, which was controlled by the inhibition of phosphorylation of p38 MAPK. These findings show that these compounds could be candidates for the novel molecular target for a skin-whitening agent.

scholarly journals Novel Quercetin Derivative of 3,7-Dioleylquercetin Shows Less Toxicity and Highly Potent Tyrosinase Inhibition Activity

2021 ◽  
Vol 22 (8) ◽  
pp. 4264
Author(s):  
Moon-Hee Choi ◽  
Seung-Hwa Yang ◽  
Da-Song Kim ◽  
Nam Doo Kim ◽  
Hyun-Jae Shin ◽  
...  
Keyword(s):  
Inhibition Mechanism ◽  
Dependent Manner ◽  
Related Protein ◽  
Tyrosinase Inhibition ◽  
Zebrafish Model ◽  
Skin Whitening ◽  
Quercetin Derivatives ◽  
Tyrosinase Related Protein

Quercetin is a well-known plant flavonol and antioxidant; however, there has been some debate regarding the efficacy and safety of native quercetin as a skin-whitening agent via tyrosinase inhibition. Several researchers have synthesized quercetin derivatives as low-toxicity antioxidants and whitening agents. However, no suitable quercetin derivatives have been reported to date. In this study, a novel quercetin derivative was synthesized by the SN2 reaction using quercetin and oleyl bromide. The relationship between the structures and activities of quercetin derivatives as anti-melanogenic agents was assessed using in vitro enzyme kinetics, molecular docking, and quenching studies; cell line experiments; and in vivo zebrafish model studies. Novel 3,7-dioleylquercetin (OQ) exhibited a low cytotoxic concentration level at >100 µg/mL (125 µM), which is five times less toxic than native quercetin. The inhibition mechanism showed that OQ is a competitive inhibitor, similar to native quercetin. Expression of tyrosinase, tyrosinase-related protein 1 (TRP-1) and tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor was inhibited in B16F10 melanoma cell lines. mRNA transcription levels of tyrosinase, TRP-1, and TRP-2 decreased in a dose-dependent manner. Melanin formation was confirmed in the zebrafish model using quercetin derivatives. Therefore, OQ might be a valuable asset for the development of novel skin-whitening agents.

Sign in / Sign up

Export Citation Format

Share Document