scholarly journals Anthocyanin Induction by Drought Stress in the Calyx of Roselle Cultivars

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1555 ◽  
Author(s):  
Jeny Hinojosa-Gómez ◽  
César San Martín-Hernández ◽  
José B. Heredia ◽  
Josefina León-Félix ◽  
Tomás Osuna-Enciso ◽  
...  
Keyword(s):  
Water Stress ◽  
Abiotic Factors ◽  
Control Level ◽  
Ultra Performance Liquid Chromatography ◽  
Anthocyanin Content ◽  
Hibiscus Sabdariffa ◽  
Chemical Profile ◽  
Moisture Condition ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content

Abiotic factors can alter the chemical profile of crops and the number of compounds they contain. In this study, the anthocyanin and anthocyanidin contents, determined by ultra-performance liquid chromatography (UPLC-MS/MS), and the colour attributes of the calyces of three cultivars of Hibiscus sabdariffa subjected to three water stress regimes during the stage of physiological maturity were investigated. The total anthocyanin content in calyx increased relative to the control content under a 65% moisture irrigation regime. Among the cultivars, UAN16-2 showed the greatest increases in the contents of cyanidin, delphinidin 3-O-glucoside, cyanidin 3-O-glucoside, and cyanidin 3-O-sambubioside. The content of cyanidin 3-O-sambubioside showed the greatest increase, increasing by 55% relative to the control level. The contents of these compounds are correlated with colour attributes such as luminosity. Water stress under the 33% moisture condition during plant development led to decreased anthocyanin contents in all of the roselle cultivars.

scholarly journals Effects of Temperature and Heating Time on Degradation and Antioxidant Activity of Anthocyanin from Roselle Petals (Hibiscus sabdariffa L.)

2020 ◽  
Vol 1 (4) ◽  
pp. 288-238
Author(s):  
Meiny Suzery ◽  
Burhan Nudin ◽  
Damar Nurwahyu Bima ◽  
Bambang Cahyono
Keyword(s):  
Antioxidant Activity ◽  
Heating Time ◽  
Heating Process ◽  
Anthocyanin Content ◽  
Hibiscus Sabdariffa ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Ic50 Value ◽  
Dpph Method ◽  
Effects Of Temperature

The effects of temperature and heating time on the degradation and antioxidant activity of anthocyanin compounds from roselle petals (Hibiscus sabdariffa L.) have been evaluated from this research. The purpose of this study is to determine, the degradation and antioxidant activity of anthocyanin compounds on variations of temperature and heating time. In this study anthocyanin was extracted from roselle petals using ethanol solvent at 25oC. Then the anthocyanin extract obtained was dissolved into aqueous solution. The solution was then heated at 40oC, 60oC and 80oC for 60 minutes. The degradation of anthocyanin compounds was analyzed by calculating the total of anthocyanin contents, while the antioxidant activity was evaluated using the DPPH method. The results showed that Total Anthocyanin Content (TAC) from the extract solution before heating was 144.28 mg/L. Meanwhile the total value of anthocyanin in the 60 minutes after heating at 400C, 600C and 800C are 108.88 mg/L, 88.84 mg/L and 63.78 mg/L respectively. Heating at 80oC decreases antioxidant activity by increasing the IC50 value to 86.8 ppm higher than the conditions before heating. The increase of temperature and length of the heating process made the anthocyanin structure degraded. Degradation was indicated by the reduction of total anthocyanin content and the decrease of antioxidant activity.

scholarly journals Efek Penambahan Sari Bunga Rosella (Hibiscus sabdariffa L.) Terhadap Kualitas Selai Lembaran Dami Nangka (Artocarpus heterophyllus)

2019 ◽  
Vol 12 (01) ◽  
pp. 21-33
Author(s):  
Veronica Angga Puspita ◽  
Tatang Sopandi
Keyword(s):  
Vitamin C ◽  
Total Content ◽  
Raw Material ◽  
Anthocyanin Content ◽  
Hibiscus Sabdariffa ◽  
Total Anthocyanin ◽  
Flower Extract ◽  
Randomized Design ◽  
Total Anthocyanin Content ◽  
Completely Randomized Design

Straw from jackfruit so far is still considered a waste that is still thrown away by most people and even often becomes a problem that can pollute the environment. Jackfruit straw contains many of the same substances as fruit flesh such as protein, crude fiber, sugar and so on. Jackfruit straw has pectin which can be used as raw material in making jam. In making jam, fruits that have high acidity are also needed. Rosella contains vitamin C and flavonoids in the form of antosanin which can be used as flavor enhancers and natural dyes. The aim of this study was to determine the addition of rosella flower concentration that produced the best jackfruit straw jam based on the level of panelist preference (texture, color, taste), anthocyanin content, vitamin C content. This study used a completely randomized design with 5 treatments consisting of concentrations 0%, 10%, 20%, 30% and 40% with 5 repetitions. Analysis carried out by ANOVA test, if there is an effect of giving rosella juice then it will be followed by Duncan's test. The results showed that the addition of 40% roselle flower extract affected the level of preference for texture, color and taste. The addition of 40% has the highest total anthocyanin content of 45.22 mg / 100g and the total content of vitamin C in jackfruit straw sheet jam is 35.11 ppm.   Keyword : Jam sheet, jackfruit, rosella essence, levels of pleasure, anthocyanin contains, vitamin C contains.

scholarly journals Optimization of total anthocyanin content and antioxidant activity of a Hibiscus sabdariffa infusion using response surface methodology

Biotecnia ◽  
2019 ◽  
Vol 21 (2) ◽  
pp. 114-122 ◽  
Author(s):  
Mayra L. Salmerón-Ruiz ◽  
J. Abraham Domínguez-Avila ◽  
J. Fernando Ayala-Zavala ◽  
Emilio Alvarez-Parrilla ◽  
Mónica A. Villegas-Ochoa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Anthocyanin Content ◽  
Hibiscus Sabdariffa ◽  
Dpph Assay ◽  
Total Anthocyanin ◽  
Preparation Conditions ◽  
Total Anthocyanin Content ◽  
Health Promoting

Hibiscus sabdariffa L. calyces are underutilized sources of health-promoting anthocyanins. Infusions are the most common way to consume them, but because anthocyanins are thermosensitive, prolonged extraction times at high temperatures may reduce their bioactivities, suggesting the need to identify optimal preparation conditions. Response surface methodology was used to establish calyces-to-water ratio (X1: 1–20 g/100 mL), temperature (X2: 70–100 °C), and time (X3: 1–30 min) that would produce an infusion with optimized total anthocyanin content (TAC) and antioxidant activity. Under optimum conditions (X1=10 g/100 mL, X2=88.7 °C, and X3=15.5 min) TAC was 132.7±7.8 mg cyanidin-3-glucoside equivalents (C3G)/100 mL, and antioxidant activity was 800.6±69.9 (DPPH assay), and 1792.0±153.5 (ABTS assay) μmol Trolox equivalents (TE)/100 mL. Predicted and experimental results were statistically similar. Identifying ideal processing conditions can promote consumption of an H. sabdariffa-based functional beverage with high anthocyanin content and antioxidant activity that exert health-promoting bioactivities on the consumer.

Inhibitory effects of bioaccessible anthocyanins and procyanidins from apple, red grape, cinnamon on α-amylase, α-glucosidase and lipase

2020 ◽  
pp. 1-9
Author(s):  
Pınar Ercan ◽  
Sedef Nehir El
Keyword(s):  
Inhibitory Activity ◽  
Digestive Enzymes ◽  
Positive Control ◽  
Anthocyanin Content ◽  
Inhibitory Effects ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Before And After ◽  
Red Grape

Abstract. The goals of this study were to determine and evaluate the bioaccessibility of total anthocyanin and procyanidin in apple (Amasya, Malus communis), red grape (Papazkarası, Vitis vinifera) and cinnamon (Cassia, Cinnamomum) using an in vitro static digestion system based on human gastrointestinal physiologically relevant conditions. Also, in vitro inhibitory effects of these foods on lipid (lipase) and carbohydrate digestive enzymes (α-amylase and α-glucosidase) were performed with before and after digested samples using acarbose and methylumbelliferyl oleate (4MUO) as the positive control. While the highest total anthocyanin content was found in red grape (164 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432 ± 177.31 mg/100 g) (p < 0.05). The anthocyanin bioaccessibilities were found as 10.2 ± 1%, 8.23 ± 0.64%, and 8.73 ± 0.70% in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57 ± 0.71%, 14.08 ± 0.74% and 18.75 ± 1.49%, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544 ± 21.94, 445 ± 15.67, 1592 ± 17.58 μg/mL, respectively), α-amylase (IC50 38.4 ± 7.26, 56.1 ± 3.60, 3.54 ± 0.86 μg/mL, respectively), and lipase (IC50 52.7 ± 2.05, 581 ± 54.14, 49.6 ± 2.72 μg/mL), respectively. According to our results apple, red grape and cinnamon have potential to inhibit of lipase, α-amylase and α-glucosidase digestive enzymes.

scholarly journals Effect of Freeze Crystallization on Quality Properties of Two Endemic Patagonian Berries Juices: Murta (Ugni molinae) and Arrayan (Luma apiculata)

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 466
Author(s):  
María Guerra-Valle ◽  
Siegried Lillo-Perez ◽  
Guillermo Petzold ◽  
Patricio Orellana-Palma
Keyword(s):  
Process Parameters ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Flavonoid Content ◽  
Total Polyphenol ◽  
Total Anthocyanin ◽  
Quality Properties ◽  
Total Anthocyanin Content ◽  
Ugni Molinae

This work studied the effects of centrifugal block freeze crystallization (CBFC) on physicochemical parameters, total phenolic compound content (TPCC), antioxidant activity (AA), and process parameters applied to fresh murta and arrayan juices. In the last cycle, for fresh murta and arrayan juices, the total soluble solids (TSS) showed values close to 48 and 54 Brix, and TPCC exhibited values of approximately 20 and 66 mg gallic acid equivalents/100 grams dry matter (d.m.) for total polyphenol content, 13 and 25 mg cyanidin-3-glucoside equivalents/100 grams d.m. for total anthocyanin content, and 9 and 17 mg quercetin equivalents/100 grams d.m. for total flavonoid content, respectively. Moreover, the TPCC retention indicated values over 78% for murta juice, and 82% for arrayan juice. Similarly, the AA presented an increase over 2.1 times in relation to the correspondent initial AA value. Thus, the process parameters values were between 69% and 85% for efficiency, 70% and 88% for percentage of concentrate, and 0.72% and 0.88 (kg solutes/kg initial solutes) for solute yield. Therefore, this work provides insight about CBFC on valuable properties in fresh Patagonian berries juices, for future applications in health and industrial scale.

scholarly journals Differences in Anthocyanin Accumulation Profiles between Teinturier and Non-Teinturier Cultivars during Ripening

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1073
Author(s):  
Meng-Bo Tian ◽  
Lin Yuan ◽  
Ming-Yuan Zheng ◽  
Zhu-Mei Xi
Keyword(s):  
Gene Expression ◽  
Plant Secondary Metabolites ◽  
Anthocyanin Synthesis ◽  
Anthocyanin Content ◽  
Anthocyanin Accumulation ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
The Individual ◽  
Relative Gene ◽  
High Expression Level

Anthocyanins are vital components of plant secondary metabolites, and are also the most important coloring substances in wine. Teinturier cultivars are rich in anthocyanins. However, the differences in anthocyanin accumulation and profiles between teinturier and non-teinturier cultivars have not been reported. In this study, Yan 73 and Dunkelfelder were selected as the experimental materials, and three non-teinturier cultivars were used for comparison. LC-MS and qRT-PCR were used to determine the individual anthocyanin contents and the relative gene expression. The results show that the total anthocyanin content of the teinturier cultivars was considerably higher than that in non-teinturier cultivars, and the levels of individual anthocyanins increased gradually during ripening. Lower ratios of modified anthocyanins were found in the teinturier cultivars, which was not only due to the high expression level of VvUFGT and VvGST4, but also due to the relatively low expression of VvOMT in these cultivars. Cluster analysis of gene expression and anthocyanin accumulation showed that VvUFGT is related to anthocyanin accumulation, and that AM1 is related to the synthesis and transport of methylated anthocyanins. Our results will be useful for further clarifying the pathways of anthocyanin synthesis, modification, and transport in teinturier cultivars.

scholarly journals ANTHOCYANIN AND ANTHOCYANIDIN CONTENT OF HIGHBUSH BLUEBERRIES CULTIVATED IN BRAZIL

2013 ◽  
Vol 31 (2) ◽  
Author(s):  
JÚLIA RIBEIRO SARKIS ◽  
ISABEL CRISTINA TESSARO ◽  
LIGIA DAMASCENO FERREIRA MAECZAK
Keyword(s):  
Gradient Elution ◽  
Vaccinium Corymbosum ◽  
Differential Method ◽  
Anthocyanin Content ◽  
Qualitative And Quantitative Analysis ◽  
Total Anthocyanin ◽  
Qualitative And Quantitative ◽  
Total Anthocyanin Content ◽  
Highbush Blueberries ◽  
Fresh Pulp

The objective of this study was to apply a HPLC methodology for qualitative and quantitative analysis of the six anthocyanidins present in fruits and to analyze the anthocyanin and anthocyanidin content of blueberries cultivated in Southern Brazil. The samples used belong to highbush blueberries (Vaccinium corymbosum) cultivars. Total anthocyanin content was determined by the pH differential method and an HPLC gradient elution system with C18 column and UV-Vis detection at 520 nm were used for separation and quantification of the anthocyanidins. Total anthocyanin content was of 128 ± 3 mg per 100 g of fresh pulp. Blueberry pulp presented 55 % of delphinidin, 8 % of cyanidin, 3 % of peonidin and 34 % of malvidin. Pelargonidin was not identified in the sample and petunidin was below the limits of quantification. The results were similar to those reported in studies using North American and European blueberries.

scholarly journals Transcriptome analyses reveal anthocyanin biosynthesis in eggplants

2018 ◽  
Author(s):  
Xi Ou Xiao ◽  
Wen qiu Lin ◽  
Ke Li ◽  
Xue Feng Feng ◽  
Hui Jin ◽  
...  
Keyword(s):  
Anthocyanin Biosynthesis ◽  
Negative Regulation ◽  
Anthocyanin Content ◽  
Biosynthesis Pathway ◽  
Rna Seq ◽  
Wild Type ◽  
Rt Pcr ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Cumulative Knowledge

We obtained a white-peel eggplant (L6-5) by EMS mutation in our previous study, whose total anthocyanin content was significantly decreased as compared with that of wild-type (WT). To analyse the anthocyanin biosynthesis mechanism in eggplants, we analysed the eggplant peel by RNA-seq in this study. The transcript results revealed upregulation of 465 genes and downregulation of 525 genes in L6-5 as compared with the WT eggplant. A total of 11 anthocyanin biosynthesis structure genes were significantly downregulated in L6-5 as compared with that in WT. Meanwhile, on the basis of the RT-PCR results of four natural eggplant cultivars, the expression pattern of 11 anthocyanin biosynthesis structure genes was consistent with the anthocyanin content. Thus, we speculated the anthocyanin biosynthesis pathway in eggplant peel. The transcript and RT-PCR results suggested positive regulation of MYB1, MYB108 and TTG8 and negative regulation of bHLH36 in anthocyanin biosynthesis. This study enhanced our cumulative knowledge about anthocyanin biosynthesis in eggplant peels.

scholarly journals Effect of drying techniques on the retention of antioxidant activities of Saskatoon berries

2013 ◽  
Vol 2 (2) ◽  
Author(s):  
Pranabendu Mitra ◽  
Venkatesh Meda ◽  
Rick Green
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Vacuum Drying ◽  
Anthocyanin Content ◽  
Dpph Radical ◽  
Total Anthocyanin ◽  
Hot Air ◽  
Total Anthocyanin Content ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

The main objective of this research was to compare the retention of antioxidant activity and total anthocyanin content of Saskatoon berries dried by freeze drying, microwave-vacuum drying, thin layer hot air drying and vacuum drying. Antioxidant activity of berry samples was determined by DPPH radical scavenging and ABTS radical scavenging, and the pH differential method was used to determine total anthocyanin content of the berry samples. The results showed that the freeze dried Saskatoon berries exhibited the highest retention of anthocyanin and antioxidant activity among the dried samples, followed by microwave-vacuum dried berries, thin layer hot air dried berries and vacuum dried berries. There were significant differences between the berry samples at P<0.05.  DPPH radical scavenging and ABTS radical scavenging were correlated linearly with an R2 value of 0.99 at P<0.05 showing their effectiveness for the determination of the antioxidant activity of the Saskatoon berries. However, the DPPH radical scavenging assay was more effective than the ABTS radical scavenging assay. The results also showed that antioxidant activity of the berries was highly correlated with the total anthocyanin content of the fruit. The reduction of anthocyanin in dried berry samples was linearly correlated with the reduction of DPPH radical scavenging with an R2 value of 0.97 at P<0.05 and, also, linearly correlated with the reduction of ABTS radical scavenging with an R2 value of 0.88 at P<0.05.

scholarly journals INFLUENCE OF IRRIGATION STRATEGIES ON THE PHYSICOCHEMICAL PROPERTIES OF ‘SYRAH’ WINE PRODUCED IN SÃO FRANCISCO VALLEY

Irriga ◽  
2018 ◽  
Vol 23 (4) ◽  
pp. 818-834 ◽  
Author(s):  
Vanessa De Souza Oliveira ◽  
Giuliano Elias Pereira ◽  
Augusto Miguel Nascimento Lima ◽  
Alessandra Monteiro Salviano ◽  
Russaika Lirio Nascimento ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Vitis Vinifera ◽  
Deficit Irrigation ◽  
Vitis Vinifera L ◽  
Anthocyanin Content ◽  
Regulated Deficit Irrigation ◽  
Total Anthocyanin ◽  
Volatile Acidity ◽  
Total Anthocyanin Content ◽  
Caixa Postal

INFLUENCE OF IRRIGATION STRATEGIES ON THE PHYSICOCHEMICAL PROPERTIES OF ‘SYRAH’ WINE PRODUCED IN SÃO FRANCISCO VALLEY     VANESSA DE SOUZA OLIVEIRA1; GIULIANO ELIAS PEREIRA2; AUGUSTO MIGUEL NASCIMENTO LIMA1; ALESSANDRA MONTEIRO SALVIANO3; RUSSAIKA LÍRIO NASCIMENTO1 E LUÍS HENRIQUE BASSOI4   1Univasf, Colegiado de Engenharia e Ambienta, Avenida Antonio Carlos Magalhães, 510, 48902-300, Juazeiro - BA, Brasil. [email protected], [email protected], [email protected] 2Embrapa Uva e Vinho, Rua Livramento, 515, Caixa Postal 130, 95701-008, Bento Gonçalves - RS, Brasil. [email protected] 3Embrapa Semiárido, BR 428 km 152, Caixa Postal 23, 56302-970, Petrolina - PE, Brasil. [email protected] 4Embrapa Instrumentação, Rua XV de Novembro, 1452, Caixa Postal 741, 13560-970, São Carlos - SP, Brasil. [email protected]     1 ABSTRACT   Deficit irrigation is used to control grapevine vegetative vigor and the grape composition can be influenced by soil water content. The minerals contained in the grapes are responsible for the physicochemical properties and stability of the wine. Therefore, the aim of this work was to evaluate the effects of irrigation strategies on the properties of ‘Syrah’ wines produced in São Francisco Valley, Brazil. The irrigation treatments used were FI (full irrigation), RDI (regulated deficit irrigation), and DI (deficit irrigation). Winemaking was performed by traditional methods, followed by stabilization and bottling. Density, alcohol content, pH, total and volatile acidity, total and free sulfur dioxide, total polyphenols index, color intensity, and total anthocyanin content were determined, as well as the contents of Ca, Mg, P, K, Na, Mn, Fe, Cu, Zn, Cd, Cr, Ni, and Pb. The properties of wines were influenced by irrigation strategies, except for the content of total anthocyanin, Mg, P, Na, Cu, and Mn. The contents of Cd, Cr, Fe, and Zn were similar in the vines. The wines demonstrated varied properties with different typicalities. Irrigation strategies can be recommended for different products, FI for young and cheap, and DI and RDI for aged and more expensive wines.   Keywords: Vitis vinifera L., grape, water availability, tropical wine.     OLIVEIRA, V. DE S.; PEREIRA, G. E.; LIMA, A. M. N.; SALVIANO, A. M.; NASCIMENTO, R. L. E BASSOI, L. H. INFLUÊNCIA DE ESTRATÉGIAS DE IRRIGAÇÃO NAS PROPRIEDADES FÍSICO-QUÍMICAS DO VINHO ‘SYRAH’ PRODUZIDO NO VALE DO SÃO FRANCISCO           2 RESUMO   A irrigação com déficit é utilizada para controlar o vigor vegetativo da videira, e a composição da uva pode ser influenciada pela umidade do solo. Os minerais presentes nas uvas são responsáveis pelas propriedades físico-químicas e pela estabilidade do vinho. Portanto, o objetivo deste trabalho foi avaliar os efeitos de estratégias de irrigação sobre as propriedades do vinho ‘Syrah’ produzido no Vale do São Francisco, Brasil. As estratégias de irrigação utilizadas foram IP (irrigação plena), IDC (irrigação com déficit controlado) e ID (irrigação com déficit). A elaboração de vinhos foi realizada por métodos tradicionais, seguido de estabilização e engarrafamento. A densidade, teor de álcool, pH, acidez total e volátil, dióxido de total e livre de enxofre, o índice total de polifenóis, a intensidade da cor, conteúdo total de antocianina e concentrações de Ca, Mg, P, K, Na, Mn, Fe, Cu e Zn, Cd, Cr, Ni e Pb foram determinadas. As propriedades dos vinhos foram influenciadas pelas estratégias de irrigação, exceto os teores totais de antocianinas, e de Mg, P, Na, Cu e Mn. Os níveis de Cd, Cr, Fe e Zn foram semelhantes nos vinhos. Os vinhos demonstraram propriedades variadas com diferentes tipicidades. As estratégias de irrigação podem ser recomendadas para a obtenção de diferentes produtos, sendo a IP para vinhos jovens e baratos, e o ID e IDC para vinhos envelhecidos e mais caros.   Palavras chave: Vitis vinifera L., uva, disponibilidade de água, vinho tropical.

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