scholarly journals Cellular Internalization of Beta-Carotene Loaded Polyelectrolyte Multilayer Capsules by Raman Mapping

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1477 ◽  
Author(s):  
Loredana F. Leopold ◽  
Oana Marișca ◽  
Ioana Oprea ◽  
Dumitrița Rugină ◽  
Maria Suciu ◽  
...  
Keyword(s):  
Beta Carotene ◽  
Bioactive Molecules ◽  
Cellular Internalization ◽  
Layer By Layer ◽  
Raman Mapping ◽  
Polyelectrolyte Multilayer ◽  
Layer Technique ◽  
Cellular Components ◽  
Layer By Layer Technique

Raman mapping is becoming a very useful tool in investigating cells and cellular components, as well as bioactive molecules intracellularly. In this study, we have encapsulated beta-carotene using a layer-by-layer technique, as a way to enhance its stability and bioavailability. Further, we have used Raman mapping to characterize the as-obtained capsules and monitor their uptake by the human retinal epithelial D407 cells. We were able to successfully map the beta-carotene distribution inside the capsules, to localize the capsules intracellularly, and distinguish between capsules and other cellular components.

scholarly journals Evaluations of polyelectrolyte multilayer membranes assembled by a dynamic layer-by-layer technique

Desalination ◽  
2008 ◽  
Vol 234 (1-3) ◽  
pp. 300-306 ◽  
Author(s):  
Shulan Ji ◽  
Guojun Zhang ◽  
Zhongzhou Liu ◽  
Yuelian Peng ◽  
Zhan Wang
Keyword(s):  
Layer By Layer ◽  
Polyelectrolyte Multilayer ◽  
Layer Technique ◽  
Multilayer Membranes ◽  
Layer By Layer Technique ◽  
Dynamic Layer

Polyelectrolyte Multilayer Film Coating and Stability at the Surfaces of Oral Prosthesis Base Polymers: an in vitro and in vivo Study

2006 ◽  
Vol 85 (1) ◽  
pp. 44-48 ◽  
Author(s):  
O. Etienne ◽  
C. Picart ◽  
C. Taddei ◽  
P. Keller ◽  
E. Hubsch ◽  
...  
Keyword(s):  
Multilayer Film ◽  
Film Coating ◽  
Human Saliva ◽  
Layer By Layer ◽  
Polyelectrolyte Multilayer ◽  
Layer Technique ◽  
Polyelectrolyte Multilayer Film ◽  
Layer By Layer Technique

A new type of coating involving a layer-by-layer technique has been recently reported. This coating is composed of a polyelectrolyte multilayer film that confers specific properties on surfaces to which it is applied. Here, we studied the applicability of such a technique to the coating of oral prostheses, by first testing the construction of polyelectrolyte multilayer films on several polymers used in oral prosthesis bases, and, subsequently, by studying the stability of these coatings in vitro, in human saliva, and in vivo in a rat model. We demonstrated that the multilayered films are able to coat the surfaces of all tested polymers completely, thus increasing their wettability. We also showed that saliva does not degrade the film after 7 days in vitro and after 4 days in vivo. Taken together, our results establish that the layer-by-layer technique is suitable for the coating of oral devices.

Pectin-Chitosan Multilayer Coated Microbeads of Diclofenac Sodium Prepared by Layer-by-Layer Technique

2014 ◽  
Vol 1060 ◽  
pp. 45-49
Author(s):  
Kamonrak Cheewatanakornkool ◽  
Pornsak Sriamornsak
Keyword(s):  
Controlled Release ◽  
Drug Release ◽  
Diclofenac Sodium ◽  
Gastric Fluid ◽  
Layer By Layer ◽  
Release Behavior ◽  
Freeze Dried ◽  
Layer Technique ◽  
Calcium Pectinate ◽  
Layer By Layer Technique

The main objective of this study was to fabricate biopolymer-based microbeads, providing enteric properties and controlled release of diclofenac sodium, using layer-by-layer technique. The calcium pectinate microbeads have been designed and coated with chitosan and pectin multilayers. Drug release was performed in simulate gastric fluid (pH 1.2) for 2 hours, followed by pH 6.8 buffer for 8 hours. The effects of chitosan concentration, number of layer and drying technique on drug release were investigated. The results showed that the calcium pectinate microbeads could be simply prepared by ionotropic gelation and then coated with chitosan and pectin solutions using layer-by-layer procedure. The diameter of the microbeads ranged from 800 to 1000 μm for air-dried samples and from 1 to 2 mm for freeze-dried samples. The freeze-dried microbeads had a rough surface and many pores inside, as observed by SEM. The microbeads coated with 4% chitosan/4% pectin revealed a slower drug release than those coated with 1% chitosan/4% pectin and demonstrated a controlled release pattern. Moreover, different drying techniques and numbers of layer also influenced drug release behavior of the prepared microbeads.

Highly transparent and self-healing films based on the dynamic Schiff base linkage

RSC Advances ◽  
2016 ◽  
Vol 6 (116) ◽  
pp. 115247-115251 ◽  
Author(s):  
Jiaoyu Ren ◽  
Yanxi Zhu ◽  
Hongyun Xuan ◽  
Xuefan Liu ◽  
Zhichao Lou ◽  
...  
Keyword(s):  
Schiff Base ◽  
Layer By Layer ◽  
Self Healing ◽  
Layer Technique ◽  
Layer By Layer Technique

In this study, we report highly transparent self-healing films based on Schiff base linkages between DF-PEG and CS via a layer-by-layer technique.

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