scholarly journals Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1335 ◽  
Author(s):  
Yannick Nuapia ◽  
Hlanganani Tutu ◽  
Luke Chimuka ◽  
Ewa Cukrowska
Keyword(s):  
Cannabis Sativa ◽  
Polar Compounds ◽  
Hot Water ◽  
Water Extraction ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Plant Materials ◽  
Hot Water Extraction ◽  
Pressurized Hot Water Extraction ◽  
Cannabinoid Compounds

Phytochemicals of Cannabis sativa mainly for the use in the different industries are that of delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). Pressurized hot water extraction (PHWE) is seen as an efficient, fast, green extraction technique for the removal of polar and semi-polar compounds from plant materials. The PHWE technique was applied to extract cannabinoid compounds from Cannabis sativa seed. Response surface methodology was used to investigate the influence of extraction time (5–60 min), extraction temperature (50–200 °C) and collector vessel temperature (25–200 °C) on the recovery of delta-9-tetrahydrocannabinol (THC), cannabinol (CBN), cannabidiol (CBD), cannabichromene (CBG) and cannabigerol (CBC) from Cannabis sativa seed by PHWE. The identification and semi quantification of cannabinoid compounds were determined using GCXGC-TOFMS. The results obtained from different extractions show that the amount of THC and CBN was drastically decreasing in the liquid extract when the temperature rose from 140 to 160 °C in the extraction cell and the collector′s vessel. The optimal conditions to extract more CBD, CBC, and CBG than THC and CBN were set at 150 °C, 160 °C and 45 min as extraction temperature, the temperature at collector vessel, and the extraction time, respectively. At this condition, the predicted and experimental ratio of THCt (THC + CBN)/CBDt (CBD + CBC+ CBG) was found to be 0.17 and 0.18, respectively. Therefore, PHWE can be seen as an alternative to the classic extraction approach as the efficiency is higher and it is environmentally friendly.

scholarly journals In Situ Decarboxylation-Pressurized Hot Water Extraction for Selective Extraction of Cannabinoids from Cannabis sativa. Chemometric Approach

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3343
Author(s):  
Yannick Nuapia ◽  
Kgomotso Maraba ◽  
Hlanganani Tutu ◽  
Luke Chimuka ◽  
Ewa Cukrowska
Keyword(s):  
Cannabis Sativa ◽  
Selective Extraction ◽  
Hot Water ◽  
Water Extraction ◽  
P Value ◽  
Hot Water Extraction ◽  
Pressurized Hot Water Extraction ◽  
Cannabinoid Compounds ◽  
Psychoactive Effects

Isolation of the therapeutic cannabinoid compounds from Cannabis Sativa L. (C. Sativa) is important for the development of cannabis-based pharmaceuticals for cancer treatment, among other ailments. The main pharmacological cannabinoids are THC and CBD. However, THC also induces undesirable psychoactive effects. The decarboxylation process converts the naturally occurring acidic forms of cannabinoids, such as cannabidiolic acid (CBDA) and tetrahydrocannabinolic acid (THCA), to their more active neutral forms, known as cannabidiol (CBD) and tetrahydrocannabinol (THC). The purpose of this study was to selectively extract cannabinoids using a novel in situ decarboxylation pressurized hot water extraction (PHWE) system. The decarboxylation step was evaluated at different temperature (80–150 °C) and time (5–60 min) settings to obtain the optimal conditions for the decarboxylation-PHWE system using response surface methodology (RSM). The system was optimized to produce cannabis extracts with high CBD content, while suppressing the THC and CBN content. The identification and quantification of cannabinoid compounds were determined using UHPLC-MS/MS with external calibration. As a result, the RSM has shown good predictive capability with a p-value < 0.05, and the chosen parameters revealed to have a significant effect on the CBD, CBN and THC content. The optimal decarboxylation conditions for an extract richer in CBD than THC were set at 149.9 °C and 42 min as decarboxylation temperature and decarboxylation time, respectively. The extraction recoveries ranged between 96.56 and 103.42%, 95.22 and 99.95%, 99.62 and 99.81% for CBD, CBN and THC, respectively.

scholarly journals Pressurized Hot Water Extraction of Okra Seeds Reveals Antioxidant, Antidiabetic and Vasoprotective Activities

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1645
Author(s):  
Eng Shi Ong ◽  
Christina Liu Ying Oh ◽  
Joseph Choon Wee Tan ◽  
Su Yi Foo ◽  
Chen Huei Leo
Keyword(s):  
Biological Activities ◽  
Vascular Inflammation ◽  
Single Step ◽  
Polyphenolic Compounds ◽  
Hot Water ◽  
Water Extraction ◽  
Extraction Temperature ◽  
Hot Water Extraction ◽  
Pressurized Hot Water Extraction ◽  
Different Parts

Abelmoschus esculentus L. Moench (okra) is a commonly consumed vegetable that consists of the seeds and peel component which are rich in polyphenolic compounds. The aim of this study is to utilize pressurized hot water extraction (PHWE) for the extraction of bioactive phytochemicals from different parts of okra. A single step PHWE was performed at various temperatures (60 °C, 80 °C, 100 °C and 120 °C) to determine which extraction temperature exhibits the optimum phytochemical profile, antioxidant and antidiabetic activities. The optimum temperature for PHWE extraction was determined at 80 °C and the biological activities of the different parts of okra (Inner Skin, Outer Skin and Seeds) were characterized using antioxidant (DPPH and ABTS), α-glucosidase and vasoprotective assays. Using PHWE, the different parts of okra displayed distinct phytochemical profiles, which consist of primarily polyphenolic compounds. The okra Seeds were shown to have the most antioxidant capacity and antidiabetic effects compared to other okra parts, likely to be attributed to their higher levels of polyphenolic compounds. Similarly, okra Seeds also reduced vascular inflammation by downregulating TNFα-stimulated VCAM-1 and SELE expression. Furthermore, metabolite profiling by LC/MS also provided evidence of the cytoprotective effect of okra Seeds in endothelial cells. Therefore, the use of PHWE may be an alternative approach for the environmentally friendly extraction and evaluation of plant extracts for functional food applications.

Optimization of ultrasonic extraction of Lycium barbarum polysaccharides using response surface methodology

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Jitian Song ◽  
Shi Dongqi ◽  
Su Hang ◽  
Feng Yongxia ◽  
Tian Wei
Keyword(s):  
High Efficiency ◽  
Hot Water ◽  
Ultrasonic Extraction ◽  
Water Extraction ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Lycium Barbarum ◽  
Hot Water Extraction

AbstractUltrasonic extraction was a new development method to achieve high-efficiency extraction of Lycium barbarum polysaccharides instead of hot water extraction. In this paper, the single factor method combined with the box Behnken design of response surface method was used to study the influence of extraction time, extraction temperature, material liquid ratio and ultrasonic power on the extraction rate of L. barbarum polysaccharide. The results indicated that the best extraction rate of L. barbarum polysaccharide was 12.54 ± 0.12% under the conditions of 80 min for extraction time, 73 °C for extraction temperature, 1 g:38 mL for material to liquid ratio, and 185 W for ultrasonic power. Under the same operating conditions, the yield of L. barbarum polysaccharide using ultrasonic extraction was 83.3%, which was higher than that hot water extraction. Moreover, the extraction time of ultrasonic extraction was only 47% of that using hot water extraction. This suggested that there was great potentials of using ultrasonic extraction in the realization of high-efficiency extraction of L. barbarum polysaccharide. The results of this study could also provide a theoretical basis for the coupling of ultrasonic extraction and ultrasonic concentration process to develop the integrated equipment of both ultrasonic extraction and ultrasonic concentration.

Effect of Ultrasonic Wave on Lentinan Extraction

2011 ◽  
Vol 291-294 ◽  
pp. 1339-1343
Author(s):  
Wen Bo Zhang ◽  
Hong Rui Li ◽  
Jun Tao ◽  
Bing Bing Dong
Keyword(s):  
Extraction Efficiency ◽  
Hot Water ◽  
Water Extraction ◽  
Extraction Technique ◽  
Orthogonal Experimental Design ◽  
Extraction Temperature ◽  
Lentinus Edodes ◽  
Extraction Condition ◽  
Hot Water Extraction ◽  
Extraction Parameters

The research in this paper optimized the extraction technique of lentinan with ultrasonic assistant method on the basis of hot water extraction technique, and investigated the promoting function of ultrasound to polysaccharides extraction. Extraction condition was selected by means of orthogonal experimental design, four factors and three levels L9(34), after key elements were respectively chosen through single factor experiments. Comparison between optimal extraction parameters of two method, hot water extraction technique and ultrasonic assistant extraction technique, showed decreased extraction temperature and significantly shortened extraction time, which existed in the second means, improved the extraction efficiency. Lentinus edodes polysaccharide extracted with ultrasonic assistant technique, the extraction rate and polysaccharide content percentage increased 6.22% and 8.66% respectively, comparative to which extracted with hot water extraction technique.

scholarly journals Optimization of the extraction of galactoglucomannans from Pinus halepensis

Holzforschung ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Nacera Benouadah ◽  
Andrey Pranovich ◽  
Djamel Aliouche ◽  
Jalel Labidi ◽  
Stefan Willför
Keyword(s):  
High Performance ◽  
Pinus Halepensis ◽  
Hot Water ◽  
Water Extraction ◽  
Chemical Degradation ◽  
Extraction Time ◽  
Size Exclusion ◽  
Hot Water Extraction ◽  
Extraction Processes ◽  
Extraction Parameters

AbstractThe effectiveness of pressurized hot-water extraction conditions for obtaining galactoglucomannans (GGMs) from Pinus halepensis suitable for applications like coatings and films packaging was investigated. For this purpose, high molar masses with high yields are required, presenting a serious challenge for hot-water extraction processes. The extraction of GGMs was carried out in an accelerated solvent extractor (ASE) and the isolation was performed by precipitation in ethanol. Three temperatures in the range 160–180 °C and five extraction times 5–90 min were tested in order to optimize extraction parameters of GGMs, avoiding thermal and chemical degradation in hot-water. Total dissolved solids (TDS) were determined gravimetrically after freeze-drying and weight average molar masses (Mw) were determined by high-performance size exclusion chromatography (HPSEC). Total non-cellulosic carbohydrates were determined by gas chromatography (GC) after acid methanolysis. Free monomers were additionally analyzed by GC. Lignin in water extracts was measured by an ultraviolet (UV) method. Acetic acid was determined after alkaline hydrolysis of acetyl groups and analyzed by HPSEC. The main parameters influencing the extraction processes of the GGMs, namely, extraction time and temperature were studied. Optimal extraction parameters of GGMs were identified at 170 °C and 20 min extraction time, with average Mw of extracted fraction of 7 kDa leading to a GGM yield of approximately 56 ${\text{mgg}}_{\text{o}.\text{d}.\text{m}}^{-1}$, corresponding to 6% on dry wood basis.

scholarly journals Simultaneous extraction of phenolics and essential oil from peppermint by pressurized hot water extraction

2018 ◽  
Vol 56 (1) ◽  
pp. 200-207 ◽  
Author(s):  
Mustafa Çam ◽  
Esma Yüksel ◽  
Hamza Alaşalvar ◽  
Bülent Başyiğit ◽  
Hülya Şen ◽  
...  
Keyword(s):  
Essential Oil ◽  
Hot Water ◽  
Water Extraction ◽  
Hot Water Extraction ◽  
Simultaneous Extraction ◽  
Pressurized Hot Water Extraction
Sign in / Sign up

Export Citation Format

Share Document