scholarly journals Extraction Methods Affect the Structure of Goji (Lycium barbarum) Polysaccharides

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 936
Author(s):  
Shengyi Zhou ◽  
Atikur Rahman ◽  
Junhui Li ◽  
Chaoyang Wei ◽  
Jianle Chen ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell Wall ◽  
Extraction Methods ◽  
Hot Water ◽  
Water Extraction ◽  
Substantial Part ◽  
Cell Wall Polysaccharides ◽  
Lycium Barbarum ◽  
Hot Water Extraction ◽  
Rhamnogalacturonan I

Polysaccharides are considered to be the most important active substances in Goji. However, the structure of polysaccharides varies according to the extraction methods applied, and the solution used to prepare Goji polysaccharides (LBPs) were limited. Thus, it is important to clarify the connection between extraction methods and structure of Goji polysaccharide. In view of the complex composition of cell wall polysaccharides and the various forms of interaction, different extraction methods will release different parts of the cell wall. The present study compared the effects of different extraction methods, which have been used to prepare different types of plant cell wall polysaccharides based on various sources, on the structure of cell-wall polysaccharides from Goji, by the single separate use of hot water, hydrochloric acid (0.4%) and sodium hydroxide (0.6%), at both high and low temperatures. Meanwhile, in order to explore the limitations of single extraction, sequential extraction methods were applied. Structural analysis including monosaccharide analysis, GPC-MALLS, AFM and 1H-NMR suggested the persistence of more extensively branched rhamnogalacturonan I (RG-I) domains in the procedures involving low-temperature-alkali, while procedures prepared by high-temperature-acid contains more homogalacturonan (HG) regions and results in the removal of a substantial part of the side chain, specifically the arabinan. A kind of acidic heteropolysaccharide was obtained by hot water extraction. SEC-MALLS and AFM confirmed large-size polymers with branched morphologies in alkali-extracted polysaccharides. Our results provide new insight into the extraction of Goji polysaccharides, which differ from the hot water extraction used by traditional Chinese medicine.

scholarly journals Effects of non-traditional extraction methods on extracting bioactive compounds from chaga mushroom (Inonotus obliquus) compared with hot water extraction

LWT ◽  
2019 ◽  
Vol 110 ◽  
pp. 80-84 ◽  
Author(s):  
Ah Young Hwang ◽  
Si Chang Yang ◽  
Jaecheol Kim ◽  
Taehwan Lim ◽  
Hyunnho Cho ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Extraction Methods ◽  
Hot Water ◽  
Water Extraction ◽  
Inonotus Obliquus ◽  
Hot Water Extraction

Optimization of ultrasonic extraction of Lycium barbarum polysaccharides using response surface methodology

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Jitian Song ◽  
Shi Dongqi ◽  
Su Hang ◽  
Feng Yongxia ◽  
Tian Wei
Keyword(s):  
High Efficiency ◽  
Hot Water ◽  
Ultrasonic Extraction ◽  
Water Extraction ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Lycium Barbarum ◽  
Hot Water Extraction

AbstractUltrasonic extraction was a new development method to achieve high-efficiency extraction of Lycium barbarum polysaccharides instead of hot water extraction. In this paper, the single factor method combined with the box Behnken design of response surface method was used to study the influence of extraction time, extraction temperature, material liquid ratio and ultrasonic power on the extraction rate of L. barbarum polysaccharide. The results indicated that the best extraction rate of L. barbarum polysaccharide was 12.54 ± 0.12% under the conditions of 80 min for extraction time, 73 °C for extraction temperature, 1 g:38 mL for material to liquid ratio, and 185 W for ultrasonic power. Under the same operating conditions, the yield of L. barbarum polysaccharide using ultrasonic extraction was 83.3%, which was higher than that hot water extraction. Moreover, the extraction time of ultrasonic extraction was only 47% of that using hot water extraction. This suggested that there was great potentials of using ultrasonic extraction in the realization of high-efficiency extraction of L. barbarum polysaccharide. The results of this study could also provide a theoretical basis for the coupling of ultrasonic extraction and ultrasonic concentration process to develop the integrated equipment of both ultrasonic extraction and ultrasonic concentration.

scholarly journals The Effect of Temperature on Pressurised Hot Water Extraction of Pharmacologically Important Metabolites as Analysed by UPLC-qTOF-MS and PCA

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
B. S. Khoza ◽  
L. Chimuka ◽  
E. Mukwevho ◽  
P. A. Steenkamp ◽  
N. E. Madala
Keyword(s):  
Antioxidant Activities ◽  
Pure Water ◽  
Principal Component ◽  
Extraction Process ◽  
Extraction Methods ◽  
Hot Water ◽  
Water Extraction ◽  
Effect Of Temperature ◽  
Extraction Temperature ◽  
Hot Water Extraction

Metabolite extraction methods have been shown to be a critical consideration for pharmacometabolomics studies and, as such, optimization and development of new extraction methods are crucial. In the current study, an organic solvent-free method, namely, pressurised hot water extraction (PHWE), was used to extract pharmacologically important metabolites from driedMoringa oleiferaleaves. Here, the temperature of the extraction solvent (pure water) was altered while keeping other factors constant using a homemade PHWE system. Samples extracted at different temperatures (50, 100, and 150°C) were assayed for antioxidant activities and the effect of the temperature on the extraction process was evaluated. The samples were further analysed by mass spectrometry to elucidate their metabolite compositions. Principal component analysis (PCA) evaluation of the UPLC-MS data showed distinctive differential metabolite patterns. Here, temperature changes during PHWE were shown to affect the levels of metabolites with known pharmacological activities, such as chlorogenic acids and flavonoids. Our overall findings suggest that, if not well optimised, the extraction temperature could compromise the “pharmacological potency” of the extracts. The use of MS in combination with PCA was furthermore shown to be an excellent approach to evaluate the quality and content of pharmacologically important extracts.

scholarly journals Effects of different extraction methods on the properties of pine cone polysaccharides from Pinus koraiensis

BioResources ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. 9945-9956
Author(s):  
Qiang Zhao ◽  
Jie Qin ◽  
Hairong Wang ◽  
Jingyu Wang ◽  
Xueming Zhang
Keyword(s):  
Monosaccharide Composition ◽  
Extraction Process ◽  
Extraction Methods ◽  
Hot Water ◽  
Water Extraction ◽  
Pinus Koraiensis ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Hot Water Extraction ◽  
Pine Cone

In order to realize the resource utilization of the Pinus koraiensis pine cone, the polysaccharides were obtained by a traditional hot water extraction process, and the optimal extraction process was confirmed. The effects of a traditional hot water, alkali, and ultrasound assisted extraction on the yield and properties of polysaccharides were studied. The structure, monosaccharide composition, and content of polysaccharides obtained by different extraction methods were analyzed by means of Fourier infrared chromatography and liquid chromatography. Effects of polysaccharides extracted by different methods on the antimicrobial activity of Staphylococcus aureus and Escherichia coli were studied. The scavenging effect of DPPH free radicals were also examined. The optimum condition of the hot water extraction was identified as an extraction temperature of 100 °C, a ratio of material to water at 1:16 (w/v), an extraction time of 4 h, and a yield of polysaccharide at 10.2%. In comparison, the yield of the pine cone polysaccharide was improved significantly to 29.1% by using NaOH solution extraction method. The FTIR spectra of polysaccharides showed that the samples possessed the typical chemical structure of a polysaccharide.

Influence of hot water extraction on cell wall and OSB strand mechanics

2017 ◽  
Vol 51 (6) ◽  
pp. 1307-1319 ◽  
Author(s):  
J. J. Paredes ◽  
S. Shaler ◽  
C. Howell ◽  
J. Jakes
Keyword(s):  
Cell Wall ◽  
Hot Water ◽  
Water Extraction ◽  
Hot Water Extraction
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