scholarly journals A New Family of Diverse Skin Peptides from the Microhylid Frog Genus Phrynomantis

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 912
Author(s):  
Constantijn Raaymakers ◽  
Benoit Stijlemans ◽  
Charlotte Martin ◽  
Shabnam Zaman ◽  
Steven Ballet ◽  
...  

A wide range of frogs produce skin poisons composed of bioactive peptides for defence against pathogens, parasites and predators. While several frog families have been thoroughly screened for skin-secreted peptides, others, like the Microhylidae, have remained mostly unexplored. Previous studies of microhylids found no evidence of peptide secretion, suggesting that this defence adaptation was evolutionarily lost. We conducted transcriptome analyses of the skins of Phrynomantis bifasciatus and Phrynomantis microps, two African microhylid species long suspected to be poisonous. Our analyses reveal 17 evolutionary related transcripts that diversified from to those of cytolytic peptides found in other frog families. The 19 peptides predicted to be processed from these transcripts, named phrynomantins, show a striking structural diversity that is distinct from any previously identified frog skin peptide. Functional analyses of five phrynomantins confirm the loss of a cytolytic function and the absence of insecticidal or proinflammatory activity, suggesting that they represent an evolutionary transition to a new, yet unknown function. Our study shows that peptides have been retained in the defence poison of at least one microhylid lineage and encourages research on similarly understudied taxa to further elucidate the diversity and evolution of skin defence molecules.

2018 ◽  
Vol 18 (7) ◽  
pp. 591-610 ◽  
Author(s):  
Chiara Testa ◽  
Anna Maria Papini ◽  
Michael Chorev ◽  
Paolo Rovero

The long-lasting impetus to design novel modes of macrocyclization, and their implementation into a wide range of bioactive peptides, originates from their contributions to the restriction of conformational space and the stabilization of preferential bioactive conformations that support higher efficacy and binding affinity to cognate macromolecular targets, improved specificity and lowering susceptibility to enzymatic degradation processes. Introducing CuI-catalyzed azide-alkyne cycloaddition (CuAAC), a prototypical click reaction, to the field of peptide sciences as a bio-orthogonal reaction that generates a disubstituted-[1,2,3]triazol-1-yl moiety as a pseudopeptidic bond that is peptidomimetic in nature, paved the way to its widespread application as a new and promising mode of macrocyclization. This review presents the state-of-art of CuAAC-mediated macrocyclization as it applies to an expansive range of bioactive peptides and explores the relationship among the structural diversity of CuAACmediated cyclizations, biological activities and conformations.


Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1492
Author(s):  
Francisco G. Blanco ◽  
Natalia Hernández ◽  
Virginia Rivero-Buceta ◽  
Beatriz Maestro ◽  
Jesús M. Sanz ◽  
...  

Bacterial biopolymers are naturally occurring materials comprising a wide range of molecules with diverse chemical structures that can be produced from renewable sources following the principles of the circular economy. Over the last decades, they have gained substantial interest in the biomedical field as drug nanocarriers, implantable material coatings, and tissue-regeneration scaffolds or membranes due to their inherent biocompatibility, biodegradability into nonhazardous disintegration products, and their mechanical properties, which are similar to those of human tissues. The present review focuses upon three technologically advanced bacterial biopolymers, namely, bacterial cellulose (BC), polyhydroxyalkanoates (PHA), and γ-polyglutamic acid (PGA), as models of different carbon-backbone structures (polysaccharides, polyesters, and polyamides) produced by bacteria that are suitable for biomedical applications in nanoscale systems. This selection models evidence of the wide versatility of microorganisms to generate biopolymers by diverse metabolic strategies. We highlight the suitability for applied sustainable bioprocesses for the production of BC, PHA, and PGA based on renewable carbon sources and the singularity of each process driven by bacterial machinery. The inherent properties of each polymer can be fine-tuned by means of chemical and biotechnological approaches, such as metabolic engineering and peptide functionalization, to further expand their structural diversity and their applicability as nanomaterials in biomedicine.


2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Yi Liu ◽  
Guo-Bin Zhuang ◽  
Xue-Zhi Zhou

High-mobility group box 1 (HMGB1) is a nuclear protein that can also act as an extracellular trigger of inflammation, proliferation, and migration in eye diseases. It induces signaling pathways by binding to the receptor for advanced glycation end products (RAGE) and Toll-like receptors (TLRs) 2, 4, and 9. This proinflammatory activity is considered to be important in the pathogenesis of a wide range of ocular diseases resulting from hemodynamic changes, presence of neovascular endothelial cells, secretion of intraocular immune factors or inflammation, and apoptosis of retinal cell layers. Further work is needed to elucidate in detail how HMGB1 contributes to ocular disease and how its damaging activity can be modulated. In this review, we summarize current knowledge on HMGB1 as a ligand that can evoke inflammation and immune responses in ocular diseases.


2013 ◽  
Vol 2013 (CICMT) ◽  
pp. 000014-000018 ◽  
Author(s):  
M. Osada ◽  
T. Sasaki

We present a novel procedure for ceramic nanocoating using oxide nanosheet as a building block. A variety of oxide nanosheets (such as Ti1−δO2, MnO2 and perovsites) were synthesized by delaminating appropriate layered precursors into their molecular single sheets. These nanosheets are exceptionally rich in both structural diversity and electronic properties, with potential applications including conductors, semiconductors, insulators, and ferromagnets. Another attractive aspect is that nanosheets can be organized into various nanoarchitectures by applying solution-based synthetic techniques involving electrostatic layer-by-layer assembly and Langmuir-Blodgett deposition. It is even possible to tailor superlattice assemblies, incorporating into the nanosheet galleries with a wide range of materials such as organic molecules, polymers, and inorganic/metal nanoparticles. Sophisticated functionalities or paper-like devices can be designed through the selection of nanosheets and combining materials, and precise control over their arrangement at the molecular scale.


2020 ◽  
Vol 98 (10) ◽  
pp. 953-958
Author(s):  
Amin Motamedinasab ◽  
Azam Anbaraki ◽  
Davood Afshar ◽  
Mojtaba Jafarpour

The general parasupersymmetric annihilation operator of arbitrary order does not reduce to the Kornbluth–Zypman general supersymmetric annihilation operator for the first order. In this paper, we introduce an annihilation operator for a parasupersymmetric harmonic oscillator that in the first order matches with the Kornblouth–Zypman results. Then, using the latter operator, we obtain the parasupercoherent states and calculate their entanglement, uncertainties, and statistics. We observe that these states are entangled for any arbitrary order of parasupersymmetry and their entanglement goes to zero for the large values of the coherency parameter. In addition, we find that the maximum of the entanglement of parasupercoherent states is a decreasing function of the parasupersymmetry order. Moreover, these states are minimum uncertainty states for large and also small values of the coherency parameter. Furthermore, these states show squeezing in one of the quadrature operators for a wide range of the coherency parameter, while no squeezing in the other quadrature operator is observed at all. In addition, using the Mandel parameter, we find that the statistics of these new states are subPoissonian for small values of the coherency parameter.


1976 ◽  
Vol 50 (S9) ◽  
pp. 1-261 ◽  
Author(s):  
Richard E. Grant

The Rat Buri Limestone was sampled for silicified brachiopods at 7 localities along the southern peninsula of Thailand. From north to south these localities are: Ban Kao, Khao Phrik, Khao Tok Nam, Khao Chang, Phangnga, Ko Muk NE, and Ko Muk NW. This limestone forms steep monadnocks that project above the alluvium (or the sea at Muk Island), and lacks any clear stratigraphic succession. Fossils indicate that Permian limestones identified throughout Thailand as the Rat Buri range in age from Sakmarian through Kazanian. The brachiopods from the peninsular localities indicate a late Artinskian (Baigendzinian) age and are correlated with the lower Byro Group of Western Australia, the Bitauni fauna of Timor, the upper Amb Formation in the Salt Range of Pakistan, the Lower Permian in the Karakorum Range, and the Trogkofel Limestone of the Karawanken Range in Yugoslavia. Western Hemisphere correlations are with the Copacabana Group of Peru and Bolivia and, very tenuously, with the topmost Cathedral Mountain or the lower Road Canyon Formations in West Texas.Analyses of life habits of the brachiopods indicate the following: Ban Kao lay nearest the Permian shore; the Rat Buri region was under shallow and fairly clear water, perhaps offshore from a reef; Phangnga was a muddy environment with many spiny and attached forms; Ko Muk was also fairly clear, and an especially favorable place for the growth of brachiopods. Sampling efficiency ranges from rather poor (Index .30) to very good (Index .75) with an overall index of .85 for the entire fauna. The Permian Index indicates that these faunas lived under tropical conditions, but the presence of certain genera suggests that seaways were open to Boreal regions.The brachiopod fauna consists of 109 species and 81 genera, of which one family, 15 genera and 71 species are new; 78 of these genera are considered here. The new genera (with family position in parentheses) are:Nematocrania(Craniidae);Demonedys(Chonetidae);Stictozoster(Productellidae);Comuquia, Dyschrestia(Overtoniidae);Incisius(Incisiidae, new family);Caricula, Gratiosina(Marginiferidae);Bibatiola, Celebetes(Chonetellidae);Stereochia(Dictyoclostidae);Litocothia(Lyttoniidae);Goleomyxa(Atriboniidae);Cruricella(Ambocoeliidae);Tipispirifer(Cyrtospiriferidae).Certain genera and species were selected for functional analyses. The lophophore ofIncisiusis interpreted as a filled-in schizolophe. The muscles of the Ambocoeliidae are reconstructed with a set of adjustor muscles designed to raise the shell to allow it to gape. Life position and muscle arrangement ofParalyttonia(and by analogy,Rigbyella)are reconstructed. The mode of growth and possible function of the stegidial plates ofTipispiriferare presented and, in the same vein, previous interpretations are the sequence of growth in the stegidium of the Devonian genusSphenospiraare criticized and analyzed. The cardinalia ofCleiothyridinaare interpreted with regard to muscle attachment, and the apical perforation is compared to the cardinal process of other brachiopods. The lophophore ofChonetinais reconstructed as a ptycholophe whose direction of growth is determined by the position and configuration of the anderidia.Derbyiaand other Orthotetacea are depicted as having attached to the substrate by byssus-like pedicular fibers, and thus were able to cling to loose sediment.


1991 ◽  
Vol 419 (2) ◽  
pp. 115-129 ◽  
Author(s):  
Guido Rindi ◽  
Shimon Efrat ◽  
Mohammad A. Ghatei ◽  
Stephen R. Bloom ◽  
Enrico Solcia ◽  
...  

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2095 ◽  
Author(s):  
Yi Qiu ◽  
Wen-Jian Lan ◽  
Hou-Jin Li ◽  
Liu-Ping Chen

Linear triquinane sesquiterpenoids represent an important class of natural products. Most of these compounds were isolated from fungi, sponges, and soft corals, and many of them displayed a wide range of biological activities. On account of their structural diversity and complexity, linear triquinane sesquiterpenoids present new challenges for chemical structure identification and total synthesis. 118 linear triquinane sesquiterpenoids were classified into 8 types, named types I–VIII, based on the carbon skeleton and the position of carbon substituents. Their isolation, structure elucidations, biological activities, and chemical synthesis were reviewed. This paper cited 102 articles from 1947 to 2018.


1988 ◽  
Vol 66 (12) ◽  
pp. 2611-2617 ◽  
Author(s):  
Peter L. Davies ◽  
Choy L. Hew ◽  
Garth L. Fletcher

Many marine teleosts have adapted to ice-laden seawater by evolving antifreeze proteins and glycoproteins. These proteins are synthesized in the liver for export to the blood where they circulate at levels of up to 20 mg/mL. There are at least four distinct antifreeze protein classes differing in carbohydrate content, amino acid composition, protein sequence, and secondary structure. In addition to antifreeze structural diversity, fish species differ considerably with respect to mechanisms controlling seasonal regulation of plasma antifreeze concentrations. Some species synthesize antifreeze proteins immediately before the onset of freezing conditions, some synthesize them in response to such conditions, whereas others possess high concentrations all year. Endogenous rhythms, water temperature, photoperiod, and pituitary hormones have all been implicated as regulators of plasma antifreeze protein levels. The structural diversity of antifreeze proteins and their occurrence in a wide range of fish species suggest that they evolved separately and recently during Cenozoic glaciation. Invariably, the genes coding for these antifreeze proteins are amplified, sometimes as long tandem arrays, suggesting intense selective pressure to produce large amounts of protein. The distribution of antifreeze gene types among fish species suggests that they could serve as important tools for studying phylogenetic relationships.


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