scholarly journals Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 409
Author(s):  
Ning-Hui Ma ◽  
Jing Guo ◽  
Si-Han Xu Chen ◽  
Xiu-Rong Yuan ◽  
Tong Zhang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Hplc Analysis ◽  
Hplc Method ◽  
Antioxidant Compounds ◽  
Dpph Assay ◽  
Bamboo Leaves ◽  
Phyllostachys Nigra ◽  
Further Development

Bamboo leaves of Phyllostachys nigra (PN), Lophatherum gracile (LG), and Pleioblastus amarus (PA) are three common herbs in China. In this work, a new high performance liquid chromatography (HPLC) method for the simultaneous determination of seven compounds in bamboo leaves has been developed; and PN, LG, and PA leaves were analyzed. PN showed four times as much chlorogenic acid (CA) than the other two, and contained the most isoorientin (iso-ORI) and isovitexin (iso-VIT) as well. The PA presented the most orientin (ORI) and LG covered a majority of cynaroside (CYN). We measured the antioxidant activity by scavenging the stable 2,2-diphenyl-1-pyridinohydrazinyl (DPPH) free radicals, and found that Luteolin (inhibitory concentration (IC)50 = 0.42 µM, LUT) and CYN (IC50 = 0.43 µM) showed 2–3 times higher antioxidant activity than iso-ORI (IC50 = 0.81 µM), ORI (IC50 = 0.84 µM), and other related antioxidant standards such as trolox (IC50 = 0.97 µM) and ascorbic acid (IC50 = 0.93 µM, VC). Among extracts, PN and PA showed considerable antioxidant activity, which was related well with the contents of CA, iso-ORI, and iso-VIT (p < 0.05). This study firstly provides evidence for functional antioxidant compounds of bamboo leaves based on statistical analysis of the HPLC analysis and DPPH assay, and it lays a foundation for its further development or utilization.

Study of Histamine Detection using Liquid Chromatography and Gas Chromatography

2021 ◽  
Vol 16 ◽  
pp. 1-9
Author(s):  
Muhammad Abdurrahman Munir ◽  
Muhammad Mukram Mohamed Mackeen ◽  
Lee Yook Heng ◽  
Khairiah Haji Badri
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Food Industry ◽  
Hplc Analysis ◽  
Hplc Method ◽  
Chromatography Analysis ◽  
Quantitation Limit ◽  
Satisfactory Recovery

Histamine is a heterocyclic amine shaped by decarboxylation of the histidine. It is a compound that lack chromophore and involatile. However, the detection of histamine is imperative due to the characteristic of histamine has given several disadvantages in food industry. This paper describes methods for histamine detection by employing high performance liquid chromatography and gas chromatography. The derivatization techniques required for both methods in order to increase the sensitivity of chromatography analysis. Two derivatizing agents were applied in this study such as 9-flourenilmethyl chloroformate (FMOC – Cl) for HPLC analysis whereas for GC analysis a N,O-bis (trimethylsilyl)acetamide (BSA) was used. Method validation was in accordance to Commission Decision 657/2002/CE. The validation of specificity, linearity, precision, accuracy, detection limit and quantitation limit results indicate that the methods were acceptable. The linear range for both methods were at 0.16 – 5.00 µg∙mL-1. The determination of histamine using GC showed the superiority of this instrument compared to HPLC. Method applicability was also checked on real sample namely mackerel in order to acquire a satisfactory recovery for both methods.

Comparative Study on Two Variants of Laghupanchamula (A Compound Ayurvedic Formulation) for Important Groups of Phytochemicals and Antioxidant Activity

2017 ◽  
Vol 2 (3) ◽  
pp. 157-163
Author(s):  
Shivani Ghildiyal ◽  
Apurva Joshi ◽  
Vinod K Joshi
Keyword(s):  
Antioxidant Activity ◽  
Comparative Study ◽  
High Performance ◽  
Hplc Analysis ◽  
Shikimic Acid ◽  
Ricinus Communis ◽  
Chemical Constituents ◽  
Tribulus Terrestris

ABSTRACT Introduction Laghupanchamula is a compound formulation prepared by combinations of roots of five herbs. Two variants of Laghupanchamula have been described in Ayurvedic classics where beside four common herbs fifth one is either Gokshura (Tribulus terrestris L.) or Eranda (Ricinus communis L.). The objective of the study is to make comparison between two variants of Laghupanchmula with respect to important group of phytochemicals and antioxidant activity to corroborate the science behind their therapeutic utility. Materials and methods Standard methods have been followed for quantitative determination of total quantity of phenols, tannins, flavonoids, and flavonols, and in vitro antioxidant activity in variants of Laghupanchmula formulations. Qualitative high-performance liquid chromatography (HPLC) analysis has also been performed to establish presence/absence of important chemical constituents in formulations. Results Additional quantity of phenols, tannins, flavonoids, and flavonols has been observed in Laghupanchamula variant containing Eranda than the variant containing Gokshura. Greater antioxidant activity has also been found in formulation containing Eranda. The HPLC analysis revealed the presence of shikimic acid, gallic acid, catechin in both formulations, but rutin has been found only in formulation containing Eranda. Conclusion From the results of experiments it has been observed that the formulation containing Eranda has more antioxidant activity as it contains more quantities of phenol, tannins, flavonoids, and flavonols than the formulation containing Gokshura. How to cite this article Ghildiyal S, Joshi A, Joshi VK. Comparative Study on Two Variants of Laghupanchamula (A Compound Ayurvedic Formulation) for Important Groups of Phytochemicals and Antioxidant Activity. J Drug Res Ayurvedic Sci 2017;2(3):157-163.

scholarly journals Antioxidant (gallic acid and quercetin) profile of Sumatran wild mangoes (Mangifera spp.): a potential source for antidegenerative medicine

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 220
Author(s):  
Fitmawati Fitmawati ◽  
Esi Resida ◽  
Sri Nur Kholifah ◽  
Rodesia Mustika Roza ◽  
Muhammad Almurdani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
High Performance ◽  
Dry Weight ◽  
Hplc Method ◽  
Flavonoid Compound ◽  
The Public ◽  
New Findings ◽  
Phenolic Analysis

Background: New findings on the potential of wild mangoes from the island of Sumatra as a source of antioxidant helps their conservation effort as it introduces their useful compounds to the public. This study aims to analyze the antioxidant profile and quantification of gallic acid and quercetin content from leaves and bark of Sumatran wild mangoes. Exploration and analysis of phytochemical constituents from 11 Sumatran wild mangoes was performed. Methods: Antioxidant activity of wild mangoes was analysed with 1,1- diphenyl-2-picryl hydroxyl (DPPH), and determination of quercetin and gallic acid content was performed by high performance liquid chromatography (HPLC) method. Total flavonoid and phenolic analysis was also performed. Curve fitting analysis used a linear regression approach. Results: The highest level of antioxidant activity, phenolic compound and flavonoid compound was found in the leaves and bark of Mangifera sp1. (MBS), the bark of M. foetida3 (var. batu) and leaves of M. torquenda, and the bark and leaves of M. sumatrana, respectively. The content of gallic acid in leaves ranged from 5.2270-35.4763 mg/g dry weight. Quercetin content of wild mangoes leaves ranged from 0.76 to 1.47 mg/g dry weight with the lowest value in M. foetida2 (var. manis) and the highest in M. laurina. Conclusion: The results obtained are expected to be useful in supporting the development of antidegenerative drugs from natural ingredients that have potential as immunomodulatory agents.

scholarly journals Phytochemical screening and antioxidant profiling of Sumatran wild mangoes (Mangifera spp.): a potential source for medicine antidegenerative effects

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 220 ◽  
Author(s):  
Fitmawati Fitmawati ◽  
Esi Resida ◽  
Sri Nur Kholifah ◽  
Rodesia Mustika Roza ◽  
Muhammad Almurdani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
High Performance ◽  
Dry Weight ◽  
Hplc Method ◽  
Flavonoid Compound ◽  
The Public ◽  
New Findings ◽  
Phenolic Analysis

Background: New findings on the potential of wild mangoes from the island of Sumatra as a source of antioxidant helps their conservation effort as it introduces their useful compounds to the public. This study aims to analyze the antioxidant profile and quantification of gallic acid and quercetin content from leaves and bark of Sumatran wild mangoes. Exploration and analysis of phytochemical constituents from 11 Sumatran wild mangoes was performed. Methods: Antioxidant activity of wild mangoes was analysed with 1,1- diphenyl-2-picryl hydroxyl (DPPH), and determination of quercetin and gallic acid content was performed by high performance liquid chromatography (HPLC) method. Total flavonoid and phenolic analysis was also performed. Curve fitting analysis used a linear regression approach. Results: The highest level of antioxidant activity, phenolic compound and flavonoid compound was found in the leaves and bark of Mangifera sp1. (MBS), the bark of M. foetida 3 (var. batu) and leaves of M. torquenda, and the bark and leaves of M. sumatrana, respectively. The content of gallic acid in leaves ranged from 5.23-35.48 mg/g dry weight. Quercetin content of wild mangoes leaves ranged from 0.76 to 1.16 mg/g dry weight with the lowest value in M. foetida 2 (var. manis) and the highest in M. laurina. Conclusion: The results obtained are expected to be useful in supporting the development of drugs that have antidegenerative effects.

scholarly journals Fractionation of Sugarcane Extracts and Determination of Phenolic Compounds and their Antioxidant Activity

2022 ◽  
Vol 34 (2) ◽  
pp. 284-288
Author(s):  
K. Kerdchan ◽  
P. Srihanam
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
High Performance ◽  
Hplc Analysis ◽  
Saccharum Officinarum ◽  
Phenolic Substance ◽  
Ethanolic Extracts ◽  
The Individual ◽  
Phytochemical Contents

Crude ethanolic extracts of four cultivars of sugarcane (Saccharum officinarum L.) were firstly prepared and then fractionated using silica gel column chromatography before the determination of their oxidative substances. The fractionated extracts were then tested for antioxidant activity by various assays. Finally, high-performance liquid chromatography (HPLC) was applied for the quantitative determination of the individual phenolic compounds. Sub-fraction 2 has the highest phytochemical contents as well as antioxidant activity. All tested phytochemicals had positively correlated to antioxidant activity. HPLC analysis showed that the phytochemicals in the fractionated extracts varied by the sugarcane cultivars. The main flavonoid substances found in the fractionated extracts were epicatechin, catechin, quercetin, resveratrol, myricetin and rutin while gallic acid was the main phenolic substance. The obtained information is useful for further studies and applications.

Rapid Method for the Determination of Vitamins A and E in Foods Using Ultra-High-Performance Liquid Chromatography

2012 ◽  
Vol 95 (2) ◽  
pp. 517-522 ◽  
Author(s):  
You -Shin Shim ◽  
Ki-Jin Kim ◽  
Dongwon Seo ◽  
Masahito Ito ◽  
Hiroaki Nakagawa ◽  
...  
Keyword(s):  
Particle Size ◽  
High Performance Liquid Chromatography ◽  
Rapid Method ◽  
High Performance ◽  
Hplc Analysis ◽  
Hplc Method ◽  
The Novel ◽  
Hplc Separation ◽  
Interday Precision

Abstract A rapid and novel ultra-HPLC (u-HPLC) method for the determination of vitamins A (retinol) and E (α-, γ-, and δ-tocopherol) in foods was validated in terms of its precision, accuracy, and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 μm, id 2 mm, and length 75 mm), followed by fluorescence detection. The recovery of retinol was more than 84.58%; the LOD and LOQ of the u-HPLC analysis were 0.015 and 0.045 mg/kg, respectively. The intraday and interday precision was less than 9.12%. The recoveries of α-, γ-, and δ-tocopherol were more than 81.37%; the LOD and the LOQ were 0.014, 0.002, and 0.001 mg/kg and 0.042, 0.005, and 0.004 mg/kg, respectively. All calibration curves had good linearity (r2 = 0.99) within the test ranges. The novel, rapid method coupled to u-HPLC can provide significant improvements in the speed, sensitivity, and resolution compared with a conventional HPLC method.

scholarly journals Antioxidant (gallic acid and quercetin) profile of Sumatran wild mangoes (Mangifera spp.): a potential source for antidegenerative medicine

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 220
Author(s):  
Fitmawati Fitmawati ◽  
Esi Resida ◽  
Sri Nur Kholifah ◽  
Rodesia Mustika Roza ◽  
Muhammad Almurdani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
High Performance ◽  
Dry Weight ◽  
Hplc Method ◽  
Flavonoid Compound ◽  
The Public ◽  
New Findings ◽  
Phenolic Analysis

Background: New findings on the potential of wild mangoes from the island of Sumatra as a source of antioxidant helps their conservation effort as it introduces their useful compounds to the public. This study aims to analyze the antioxidant profile and quantification of gallic acid and quercetin content from leaves and bark of Sumatran wild mangoes. Exploration and analysis of phytochemical constituents from 11 Sumatran wild mangoes was performed. Methods: Antioxidant activity of wild mangoes was analysed with 1,1- diphenyl-2-picryl hydroxyl (DPPH), and determination of quercetin and gallic acid content was performed by high performance liquid chromatography (HPLC) method. Total flavonoid and phenolic analysis was also performed. Curve fitting analysis used a linear regression approach. Results: The highest level of antioxidant activity, phenolic compound and flavonoid compound was found in the leaves and bark of Mangifera sp1. (MBS), the bark of M. foetida 3 (var. batu) and leaves of M. torquenda, and the bark and leaves of M. sumatrana, respectively. The content of gallic acid in leaves ranged from 5.23-35.48 mg/g dry weight. Quercetin content of wild mangoes leaves ranged from 0.76 to 1.16 mg/g dry weight with the lowest value in M. foetida 2 (var. manis) and the highest in M. laurina. Conclusion: The results obtained are expected to be useful in supporting the development of antidegenerative drugs from natural ingredients that have potential as immunomodulatory agents.

The Dtermination of Paracetamol by HPLC Validation of the Method and Application on Serum Samples

2018 ◽  
Vol 69 (3) ◽  
pp. 627-631 ◽  
Author(s):  
Viorica Ohriac (Popa) ◽  
Diana Cimpoesu ◽  
Adrian Florin Spac ◽  
Paul Nedelea ◽  
Voichita Lazureanu ◽  
...  
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Hplc Analysis ◽  
Emotional Experience ◽  
Optimum Conditions ◽  
Serum Samples ◽  
Liquid Chromatograph ◽  
Mobile Phase Flow Rate ◽  
Quantification Limit

Pain is defined as a disagreeable sensory and emotional experience related to a tissue or potential lesion. Paracetamol (Acetaminophen) is the most used non-morphine analgesic. For the determination of paracetamol we developed and validated the high performance liquid chromatography (HPLC) analysis using a Dionex Ultimate 3000 liquid chromatograph equipped with a multidimensional detector. After determining the optimum conditions of analysis (80/20 water / acetonitrile mobile phase, flow rate 1.0 mL / min, detection wavelength 245 nm) we validated the method following the following parameters: linearity of response function, linearity of results, limit (LD = 0.66 mg / mL) and quantification limit (LQ = 2.00 mg / mL), and precision. The method of determining paracetamol by HPLC was applied to 30 samples of serum collected from patients who had pain and were treated with paracetamol.

Developing a High-performance Liquid Chromatography Method for Simultaneous Determination of Loratadine and its Metabolite Desloratadine in Human Plasma

2020 ◽  
Vol 20 (13) ◽  
pp. 1053-1059
Author(s):  
Mahmoud M. Sebaiy ◽  
Noha I. Ziedan
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
High Performance ◽  
Allergic Diseases ◽  
Reversed Phase ◽  
Hplc Method ◽  
H1 Receptor ◽  
Chromatography Method

Background: Allergic diseases are considered as the major burden on public health with increased prevalence globally. Histamine H1-receptor antagonists are the foremost commonly used drugs in the treatment of allergic disorders. The target drug in this study, loratadine, belongs to this class of drugs and its biometabolite desloratadine which is also a non-sedating H1 receptor antagonist with anti-histaminic activity being 2.5 to 4 times greater than loratadine. This study aimed to develop and validate a novel isocratic Reversed-phase High-Performance Liquid Chromatography (RP-HPLC) method for rapid and simultaneous separation and determination of loratadine and its metabolite, desloratadine in human plasma. Methods: The drug extraction method from plasma was based on protein precipitation technique. The separation was carried out on a Thermo Scientific BDS Hypersil C18 column (5μm, 250 x 4.60 mm) in a mobile phase of MeOH: 0.025M KH2PO4 adjusted to pH 3.50 using orthophosphoric acid (85: 15, v/v) at an ambient temperature. The flow rate was maintained at 1 mL/min and maximum absorption was measured using the PDA detector at 248 nm. Results: The retention times of loratadine and desloratadine in plasma samples were recorded to be 4.10 and 5.08 minutes, respectively, indicating a short analysis time. Limits of detection were found to be 1.80 and 1.97 ng/mL for loratadine and desloratadine, respectively, showing a high degree of sensitivity of the method. The method was then validated according to FDA guidelines for the determination of the two analytes in human plasma. Conclusion: The results obtained indicate that the proposed method is rapid, sensitive in the nanogram range, accurate, selective, robust and reproducible compared to other reported methods.

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