scholarly journals Routine Management of Microalgae Using Autofluorescence from Chlorophyll

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4441 ◽  
Author(s):  
Toshiyuki Takahashi
Keyword(s):  
Cell Density ◽  
Environmental Changes ◽  
Algal Species ◽  
Working Hours ◽  
Industrial Applications ◽  
Density Analysis ◽  
Application Potential ◽  
Further Development ◽  
Automated Cell Counter ◽  
Cell Counter

From a high-potential biomass perspective, microalgae have recently attracted considerable attention due to their extensive application in many areas. Although studies searching for algal species with extensive application potential are ongoing, technical development for their assessment and maintenance of quality in culture are also critical and inescapable challenges. Considering the sensitivity of microalgae to environmental changes, management of algal quality is one of the top priorities for industrial applications. Helping substitute for conventional methods such as manual hemocytometry, turbidity, and spectrophotometry, this review presents an image-based, automated cell counter with a fluorescence filter to measure chlorophyll autofluorescence emitted by algae. Capturing chlorophyll-bearing cells selectively, the device accomplished precise qualification of algal numbers. The results for cell density using the device with fluorescence detection were almost identical to those obtained using hemocytometry. The automated functions of the device allow operators to reduce working hours, for not only cell density analysis but simultaneous multiparametric analysis such as cell size and algal status based on chlorophyll integrity. The automated device boldly supports further development of algal application and might contribute to opening up more avenues in the microalgal industry.

scholarly journals Potential of an Automated- and Image-Based Cell Counter to Accelerate Microalgal Research and Applications

Energies ◽  
2020 ◽  
Vol 13 (22) ◽  
pp. 6019
Author(s):  
Toshiyuki Takahashi
Keyword(s):  
Sustainable Development ◽  
Management System ◽  
Sustainable Development Goals ◽  
Environmental Changes ◽  
Development Goals ◽  
Conventional Methods ◽  
Functional Capacities ◽  
Automated Cell Counter ◽  
Cell Counter ◽  
Work Burden

Efforts to achieve Sustainable Development Goals (SDGs) have resulted in enhancement of the position of microalgae in feedstocks for food, feed, healthcare, and biofuels. However, stabile microalgal biorefineries require a sustainable and reliable management system of microalgae, which are sensitive to environmental changes. To expand microalgal applicability, assessment and maintenance of microalgal quality are crucial. Compared with conventional methods, including hemocytometry and turbidity, an automated- and image-based cell counter contributes to the establishment of routine management of microalgae with reduced work burden. This review presents the principle of an automated cell counter and highlights the functional capacities of the device for microalgal management. The method utilizing fluorescence function to evaluate the chlorophyll integrity of microalgae may lay the groundwork for making a large variety of microalgal biorefineries, creating an important step toward achieving SDGs.

Validation of cell density and viability assays using Cedex automated cell counter

Biologicals ◽  
2010 ◽  
Vol 38 (3) ◽  
pp. 393-400 ◽  
Author(s):  
Li-Chun Huang ◽  
Wendy Lin ◽  
Machiko Yagami ◽  
Daphne Tseng ◽  
Emily Miyashita-Lin ◽  
...  
Keyword(s):  
Cell Density ◽  
Automated Cell Counter ◽  
Cell Counter

scholarly journals The Management of Human Resources Development in Increasing the Quality of Islamic Education Institutions

AL-TA LIM ◽  
2020 ◽  
Vol 27 (2) ◽  
pp. 166-178
Author(s):  
Elvi Rahmi ◽  
Achmad Patoni ◽  
Sulistyorini Sulistyorini
Keyword(s):  
Elementary School ◽  
Human Resources ◽  
School Environment ◽  
Job Training ◽  
Environmental Changes ◽  
Participant Observation ◽  
Working Hours ◽  
Educational Institutions ◽  
Is Development

Human resources are the biggest strength in the management of Islamic educational institutions. The quality of human resources is crucial right now, because competence of human resources who have knowledge and skills will be able to cope environmental changes. Teachers and employees are the key of success in educational institutions. Good or bad performance of teachers and employees will support the competitiveness of schools. Based on this phenomenon, this research reveals the development of educators and education staff in enhancing competitiveness in the Excellent Islamic Elementary School in Bukittinggi City. This research uses descriptive qualitative. Located in Excellent Islamic Elementary School Bukittinggi City. The source of data in this study is the board of trustees, principals, teachers and employees. The techniques of data collection are participant observation, in-depth interviews and documentation. Later on the collected data is analyzed using data reduction techniques, data presentation, and conclusion. All data are approved by validity through a credibility test, transferability test, dependability test and conformability test. The results showed that the development of teachers and employees is done through 1) on the job training, that is, development that takes place during working hours takes place both formally and informally. 2) Off the job training, that is development that has done specifically outside of work. As for the development carried out: a) training in the form of quantum teaching training, workshops for making learning media and parenting seminars, b) rotation position, c) Religious Development, d) Teacher working groups (KKG) and comparative studies. This development aims to improve the competencies of teachers and employees in the school environment so that they can improve the quality of Islamic educational institutions in accordance with the increasingly stringent times. 

scholarly journals Customizable Ceramic Nanocomposites Using Carbon Nanotubes

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3176
Author(s):  
Chinyere Okolo ◽  
Rafaila Rafique ◽  
Sadia Sagar Iqbal ◽  
Tayyab Subhani ◽  
Mohd Shahneel Saharudin ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Substantial Reduction ◽  
Industrial Applications ◽  
Plasma Sintering ◽  
Electrical Conductivities ◽  
Spark Plasma ◽  
Novel Strategy ◽  
Further Development ◽  
Ceramic Matrices ◽  
Ceramic Nanocomposites

A novel tweakable nanocomposite was prepared by spark plasma sintering followed by systematic oxidation of carbon nanotube (CNT) molecules to produce alumina/carbon nanotube nanocomposites with surface porosities. The mechanical properties (flexural strength and fracture toughness), surface area, and electrical conductivities were characterized and compared. The nanocomposites were extensively analyzed by field emission scanning electron microscopy (FE-SEM) for 2D qualitative surface morphological analysis. Adding CNTs in ceramic matrices and then systematically oxidizing them, without substantial reduction in densification, induces significant capability to achieve desirable/application oriented balance between mechanical, electrical, and catalytic properties of these ceramic nanocomposites. This novel strategy, upon further development, opens new level of opportunities for real-world/industrial applications of these relatively novel engineering materials.

scholarly journals Invasive Seaweeds in the Iberian Peninsula: A Contribution for Food Supply

Marine Drugs ◽  
2020 ◽  
Vol 18 (11) ◽  
pp. 560
Author(s):  
Diana Pacheco ◽  
Glacio Souza Araújo ◽  
João Cotas ◽  
Rui Gaspar ◽  
João M. Neto ◽  
...  
Keyword(s):  
Iberian Peninsula ◽  
Food Industry ◽  
Algal Species ◽  
Industrial Applications ◽  
Indigenous Species ◽  
Invasion Mechanisms ◽  
Daily Food ◽  
Research Findings ◽  
Non Indigenous Species ◽  
Nutraceutical Properties

The introduction of exotic organisms in marine ecosystems can lead to economic and ecological losses. Globally, seaweeds represent a significant part of these non-indigenous species (NIS), with 407 introduced algal species. Furthermore, the presence of NIS seaweeds has been reported as a major concern worldwide since the patterns of their potential invasion mechanisms and vectors are not yet fully understood. Currently, in the Iberian Peninsula, around 50 NIS seaweeds have been recorded. Some of these are also considered invasive due to their overgrowth characteristic and competition with other species. However, invasive seaweeds are suitable for industrial applications due to their high feedstock. Hence, seaweeds’ historical use in daily food diet, allied to research findings, showed that macroalgae are a source of nutrients and bioactive compounds with nutraceutical properties. The main goal of this review is to evaluate the records of NIS seaweeds in the Iberian Peninsula and critically analyze the potential of invasive seaweeds application in the food industry.

scholarly journals Mechanism Underlying Flow Velocity and Its Corresponding Influence on the Growth of Euglena gracilis, a Dominant Bloom Species in Reservoirs

2019 ◽  
Vol 16 (23) ◽  
pp. 4641
Author(s):  
Yi Tan ◽  
Jia Li ◽  
Linglei Zhang ◽  
Min Chen ◽  
Yaowen Zhang ◽  
...  
Keyword(s):  
Flow Velocity ◽  
Euglena Gracilis ◽  
Environmental Changes ◽  
Algal Species ◽  
Physiological Mechanisms ◽  
Algae Growth ◽  
The Impact ◽  
Stress Hysteresis ◽  
Static Flow ◽  
Flow Velocities

The effects of hydrodynamics on algae growth have received considerable attention, and flow velocity is one of the most frequently discussed factors. For Euglena gracilis, which aggregates resources and is highly resistant to environmental changes, the mechanism underlying the impact of flow velocity on its growth is poorly understood. Experiments were conducted to examine the response of algae growth to different velocities, and several enzymes were tested to determine their physiological mechanisms. Significant differences in the growth of E. gracilis were found at different flow velocities, and this phenomenon is unique compared to the growth of other algal species. With increasing flow velocity and time, the growth of E. gracilis is gradually inhibited. In particular, we found that the pioneer enzyme is peroxidase (POD) and that the main antioxidant enzyme is catalase (CAT) when E. gracilis experiences flow velocity stress. Hysteresis between total phosphorus (TP) consumption and alkaline phosphatase (AKP) synthesis was observed. Under experimental control conditions, the results indicate that flow velocities above 0.1 m/s may inhibit growth and that E. gracilis prefers a relatively slow or even static flow velocity, and this finding could be beneficial for the control of E. gracilis blooms.

Robust automated cell counter and viability measurement for engineered T cell product release

Cytotherapy ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. S69
Author(s):  
Y. Ohayon ◽  
C.M. Corl ◽  
R.M. Leskowitz ◽  
A. Malykhin ◽  
S.M. Arostegui ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Product ◽  
Product Release ◽  
Automated Cell Counter ◽  
Cell Counter
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