scholarly journals A Reversible Colorimetric and Fluorescence “Turn-Off” Chemosensor for Detection of Cu2+ and Its Application in Living Cell Imaging

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4283 ◽  
Author(s):  
Yun Hu ◽  
Aiqian Chen ◽  
Zhuo Kong ◽  
Demeng Sun
Keyword(s):  
Cell Imaging ◽  
High Selectivity ◽  
Color Change ◽  
Dual Function ◽  
Biological Applications ◽  
Sensing System ◽  
Neutral Aqueous Solution ◽  
Intracellular Changes ◽  
Living Cell Imaging ◽  
Naked Eye Detection

Dual-function chemosensors that combine the capability of colorimetric and fluorimetric detection of Cu2+ are still relatively rare. Herein, we report that a 3-hydroxyflavone derivative (E)-2-(4-(dimethylamino)styryl)-3-hydroxy-4H-chromen-4-one (4), which is a red-emitting fluorophore, could serve as a reversible colorimetric and fluorescence “turn-off” chemosensor for the detection of Cu2+. Upon addition of Cu2+ to 4 in neutral aqueous solution, a dramatic color change from yellow to purple-red was clearly observed, and its fluorescence was markedly quenched, which was attributed to the complexation between the chemosensor and Cu2+. Conditions of the sensing process had been optimized, and the sensing studies were performed in a solution of ethanol/phosphate buffer saline (v/v = 3:7, pH = 7.0). The sensing system exhibited high selectivity towards Cu2+. The limit of naked eye detection of Cu2+ was determined at 8 × 10−6 mol/L, whereas the fluorescence titration experiment showed a detection limit at 5.7 × 10−7 mol/L. The complexation between 4 and Cu2+ was reversible, and the binding constant was found to be 3.2 × 104 M−1. Moreover, bioimaging experiments showed that 4 could penetrate the cell membrane and respond to the intracellular changes of Cu2+ within living cells, which indicated its potential for biological applications.

scholarly journals A highly selective ratiometric fluorescent probe for the cascade detection of Zn2+ and H2PO4− and its application in living cell imaging

RSC Advances ◽  
2017 ◽  
Vol 7 (64) ◽  
pp. 40615-40620 ◽  
Author(s):  
Kui Du ◽  
Shizhen Niu ◽  
Li Qiao ◽  
Yandong Dou ◽  
Qing Zhu ◽  
...  
Keyword(s):  
Charge Transfer ◽  
Fluorescent Probe ◽  
Living Cell ◽  
Cell Imaging ◽  
High Selectivity ◽  
Ratiometric Sensor ◽  
Intermolecular Charge Transfer ◽  
Living Cell Imaging ◽  
Ratiometric Fluorescent Probe

A simple ratiometric sensor (L1) for the cascade detection of Zn2+ and H2PO4− with high selectivity was reported based on the intermolecular charge transfer (ICT) mechanism.

A novel rhodamine-based fluorescent probe for selective detection of ClO– and its application in living cell imaging

2018 ◽  
Vol 96 (12) ◽  
pp. 1129-1133
Author(s):  
Changping Yang ◽  
Mingliang Zuo ◽  
Xiaoli Hu ◽  
Xuelin Chen ◽  
Duoduo Zhang ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Cell Imaging ◽  
High Selectivity ◽  
Fluorescence Enhancement ◽  
Colour Change ◽  
Living Cells ◽  
Fluorescent Imaging ◽  
Selective Detection ◽  
Living Cell Imaging ◽  
Interfering Ions

A novel fluorescent rhodamine-based probe L for selective responding to ClO– has been synthesized and characterized. The spectroscopy showed that probe L can detect ClO– in aqueous solution without interaction with other interfering ions, and the detection is also evident by the colour change from colourless to reddish purple under white light. The remarkable fluorescence enhancement showed the high selectivity and sensitivity of probe L for the detection of ClO–. Furthermore, probe L was applied to intracellular fluorescent imaging of HeLa cells treated with ClO– and MTT assay showed nontoxicity in living cells.

scholarly journals Nile-Red-Based Fluorescence Probe for Selective Detection of Biothiols, Computational Study, and Application in Cell Imaging

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4718
Author(s):  
Xiang Rong ◽  
Zhong-Yong Xu ◽  
Jin-Wu Yan ◽  
Zhi-Zhong Meng ◽  
Bin Zhu ◽  
...  
Keyword(s):  
Cell Imaging ◽  
High Selectivity ◽  
Fluorescence Enhancement ◽  
Fluorescence Probe ◽  
Color Change ◽  
Computational Study ◽  
Theoretical Calculations ◽  
Nile Red ◽  
Blue Shift ◽  
Selective Detection

A new colorimetric and fluorescence probe NRSH based on Nile-red chromophore for the detection of biothiols has been developed, exhibiting high selectivity towards biothiols over other interfering species. NRSH shows a blue shift in absorption peak upon reacting with biothiols, from 587 nm to 567 nm, which induces an obvious color change from blue to pink and exhibits a 35-fold fluorescence enhancement at 645 nm in red emission range. NRSH displays rapid (<1 min) response for H2S, which is faster than other biothiols (>5 min). The detection limits of probe NRSH towards biothiols are very low (22.05 nM for H2S, 34.04 nM for Cys, 107.28 nM for GSH and 113.65 nM for Hcy). Furthermore, NRSH is low cytotoxic and can be successfully applied as a bioimaging tool for real-time monitoring biothiols in HeLa cells. In addition, fluorescence mechanism of probe NRSH is further understood by theoretical calculations.

A fluorescent probe for the specific detection of cysteine in human serum samples

2019 ◽  
Vol 11 (26) ◽  
pp. 3280-3285 ◽  
Author(s):  
Zhen Huang ◽  
Cuiyan Wu ◽  
Yaqian Li ◽  
Zile Zhou ◽  
Ruihua Xie ◽  
...  
Keyword(s):  
Human Serum ◽  
Fluorescent Probe ◽  
High Selectivity ◽  
Color Change ◽  
Eye Detection ◽  
Specific Detection ◽  
Serum Samples ◽  
Naked Eye ◽  
Turn On ◽  
Naked Eye Detection

A turn-on fluorescent probe for cysteine with high selectivity was designed and synthetized. The probe can be used for “naked-eye” detection of Cys with an obvious color change from yellow to colorless. The probe could be applied for Cys detection in real human serum.

A selective purine-based fluorescent chemosensor for the “naked-eye” detection of zinc ions (Zn2+): applications in live cell imaging and test strips

2020 ◽  
Vol 44 (35) ◽  
pp. 15195-15201 ◽  
Author(s):  
Haiyan Xu ◽  
Wei Chen ◽  
Weixia Zhang ◽  
Lixin Ju ◽  
Hongfei Lu
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
High Selectivity ◽  
Living Cells ◽  
Fluorescent Chemosensor ◽  
Zinc Ions ◽  
Eye Detection ◽  
Naked Eye ◽  
Turn On ◽  
Naked Eye Detection

In this work, a novel purine based probe PTAHN was successfully designed and synthesized. PTAHN displayed high selectivity towards Zn2+via turn-on fluorescence. What's more, PTAHN can be proficiently employed for imaging Zn2+ in living cells.

Dansyl-appended CuII-complex-based nitroxyl (HNO) sensing with living cell imaging application and DFT studies

2019 ◽  
Vol 48 (8) ◽  
pp. 2760-2771 ◽  
Author(s):  
Debjani Maiti ◽  
Abu Saleh Musha Islam ◽  
Ananya Dutta ◽  
Mihir Sasmal ◽  
Chandraday Prodhan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Copper Complex ◽  
Cell Imaging ◽  
High Selectivity ◽  
Dft Studies ◽  
Sulfur Species ◽  
Turn On ◽  
Imaging Application ◽  
Fluorescence Turn On ◽  
Living Cell Imaging

We introduce herein, a novel copper complex-based fluorescent probe[CuII(DQ468)Cl]+that exhibits a significant fluorescence turn-on response towards nitroxyl with high selectivity over other biological reactive oxygen, nitrogen and sulfur species, including nitric oxide.

Au@Ag Core/Shell Nanorods Based Colorimetric Assay for the Rapid and Selective Detection of Cyanide Anions

2019 ◽  
Vol 11 (12) ◽  
pp. 1739-1744
Author(s):  
Rui-Ling Zhang ◽  
Shi-Liang Jia ◽  
Kai-Ming Wang
Keyword(s):  
High Selectivity ◽  
Color Change ◽  
Colorimetric Assay ◽  
Water Solution ◽  
Blue Shift ◽  
Core Shell ◽  
Optimal Conditions ◽  
Sample Analysis ◽  
Aspect Ratios ◽  
Naked Eye Detection

A colorimetric assay based on Au@Ag core/shell nanorods was developed to rapidly determine cyanide in aqueous solution. The aspect ratios of Au@Ag core/shell nanorods were changed with the dissolution of metals in cyanide solution, which led to the decrease of Ag shell absorption spectra and the blue shift of longitudinal plasmon absorption band of Au core, as well as the color change of Au@Ag core/shell nanorods. In contrast to spherical Au@Ag nanoparticles with less sample analysis time (1 min per sample), the distinguish ability of this assay was proven to be higher. Under optimal conditions, the developed colorimetric assay gave a linear range of 1–200 μM for cyanide in water solution and a naked-eye detection limit of 0.5 μM. On the side, the colorimetric assay exhibited high selectivity towards cyanide over other common anions. The developed Au@Ag nanorods probes have been successfully applied to determine the level of cyanide in local lake, river and industrial water samples.

Highly Cu2+-sensitive and selective colorimetric and fluorescent probes: Utilizations in batch, flow analysis and living cell imaging

2017 ◽  
Vol 241 ◽  
pp. 868-878 ◽  
Author(s):  
Yordkhuan Tachapermpon ◽  
Sumonmarn Chaneam ◽  
Adisri Charoenpanich ◽  
Jitnapa Sirirak ◽  
Nantanit Wanichacheva
Keyword(s):  
Fluorescent Probes ◽  
Living Cell ◽  
Cell Imaging ◽  
Flow Analysis ◽  
Living Cell Imaging
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