scholarly journals Oxidized Dextran as a Macromolecular Crosslinker Stabilizes the Zein/Caseinate Nanocomplex for the Potential Oral Delivery of Curcumin

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4061 ◽  
Author(s):  
Nikolas J. Rodriguez ◽  
Qiaobin Hu ◽  
Yangchao Luo
Keyword(s):  
Bioactive Compounds ◽  
Oral Delivery ◽  
Colloidal Stability ◽  
Schiff Base Complex ◽  
Spherical Shape ◽  
Heating Time ◽  
Morphological Observation ◽  
Hydrophobic Core ◽  
Uniform Size ◽  
Oxidized Dextran

In this study, we prepared complex nanoparticles from a combination of two proteins and one polysaccharide for the encapsulation and delivery of lipophilic bioactive compounds. Two proteins, zein and sodium caseinate (NaCas), provided a hydrophobic core for the encapsulation of a lipophilic compound (curcumin), while a polysaccharide dialdehyde, oxidized dextran, served as the coating material and macromolecular crosslinker to create covalent linkage with two proteins for stabilization purposes. The heating time and crosslinker concentration were optimized to achieve the desirable colloidal stability in simulated gastric and intestinal fluids. Our results suggested that heating time played a more important role than the concentration of oxidized dextran. The optimized complex nanoparticles had a particle size of around 150 nm with a PDI < 0.1 and negative surface charge. Morphological observation by transmission electron microscopy revealed a spherical shape and uniform size distribution. Fourier transform infrared and fluorescence spectroscopies evidenced the formation of Schiff base complex, confirming the validity of covalent crosslinking. Furthermore, the complex nanoparticles demonstrated superior encapsulation properties for curcumin, showing an efficiency of >90% at 10% loading. A rather slow kinetic release profile of curcumin from complex nanoparticles was observed under simulated gastrointestinal conditions. The complex nanoparticles prepared from zein, NaCas, and oxidized dextran hold promising potential for the oral delivery of lipophilic bioactive compounds.

scholarly journals Biosynthesis of CeO2 Nanoparticles Using Egg White and Their Antibacterial and Antibiofilm Properties on Clinical Isolates

Crystals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 584
Author(s):  
Shalendra Kumar ◽  
Faheem Ahmed ◽  
Nagih M. Shaalan ◽  
Osama Saber
Keyword(s):  
Bacterial Infections ◽  
Antibacterial Properties ◽  
Spherical Shape ◽  
Inhibition Zone ◽  
Egg White ◽  
Ceo2 Nanoparticles ◽  
Inorganic Materials ◽  
Ambient Conditions ◽  
Uniform Size ◽  
Tem Analysis

Bio-inspired synthesis is a novel and attractive environmentally friendly route to generating inorganic materials. In this work, the preparation of CeO2 NPs using egg white and investigation of their antibacterial properties both in liquid and solid growth medium against Escherichia coli and Staphylococcus aureus bacteria were reported. The CeO2 nanoparticles were characterized using X-ray diffraction (XRD), Field emission transmission electron microscope (FETEM), UV-Vis, Raman, and antibacterial measurements. The results from XRD and TEM analysis showed that the prepared nanoparticles were a single phase in the nano regime (5–7 nm) with spherical shape and uniform size distribution. Optical properties reflected the characteristics peaks of CeO2 in the UV-Vis range with a bandgap ~2.80 eV. The antibacterial activity of the synthesized NPs was achieved under ambient conditions with different bacteria and the results showed that the properties were different for both the bacteria. The highest activity with an inhibition zone of about 22 mm against S. aureus was obtained as compared with the 19 mm zone of inhibition obtained with E.coli. This finding will be of major significance that indicates a possibility to develop CeO2 NPs as antibacterial agents against extensive microorganisms to control and prevent the spread and persistence of bacterial infections.

Control of Morphology of YBO3:Eu by a Mild Hydrothermal Process

2005 ◽  
Vol 475-479 ◽  
pp. 1829-1832
Author(s):  
Ling He ◽  
Yu Hua Wang
Keyword(s):  
Solid State ◽  
Nmr Spectra ◽  
Single Phase ◽  
Hydrothermal Process ◽  
Spherical Shape ◽  
Solid State Reactions ◽  
Main Peak ◽  
Photoluminescence Intensity ◽  
Uniform Size ◽  
Excitation Peak

The phosphors YBO3:Eu with different morphology was successfully realized by mild hydrothermal process with different reaction condition. Highly spherical shape, uniform size and non-agglomeration of YBO3:Eu phosphors particle was obtained at 260°C,which is about 850°C lower than that by solid state reactions. The result of XRD showed that all samples were single phase with vaterite type structure. From IR and NMR spectra, it could be concluded that YBO3 contains mainly BO4 tetrahedra which is in favor for absorption VUV excitation. Monitoring by 598nm emission, the main excitation peak at about 247nm was observed in the excitation spectrum of spherical Y0.95Eu0.05BO3. Correspondingly, under 247nm excitation, the main peak at about 598nm was observed. The photoluminescence intensity (5D0→7F1 transition of Eu3+) of spherical shape of Y0.95Eu0.05BO3 was 1.5 times in comparison with which prepared by solid-state reactions. The best photoluminescence intensity was obtained when the concentration of Eu3+ is at 20 at. %. It indicated that hydrothermal method have a potential in the preparing various particles phosphors for application in displays and lamps.

scholarly journals One-Pot Method for Preparation of Magnetic Multi-Core Nanocarriers for Drug Delivery

Materials ◽  
2019 ◽  
Vol 12 (3) ◽  
pp. 540 ◽  
Author(s):  
Črt Dragar ◽  
Tanja Potrč ◽  
Sebastjan Nemec ◽  
Robert Roškar ◽  
Stane Pajk ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Aqueous Phase ◽  
Colloidal Stability ◽  
Drug Loading ◽  
Spherical Shape ◽  
Surfactant Solution ◽  
Time Frame ◽  
One Pot ◽  
Magnetic Nanocarriers

The development of various magnetically-responsive nanostructures is of great importance in biomedicine. The controlled assembly of many small superparamagnetic nanocrystals into large multi-core clusters is needed for effective magnetic drug delivery. Here, we present a novel one-pot method for the preparation of multi-core clusters for drug delivery (i.e., magnetic nanocarriers). The method is based on hot homogenization of a hydrophobic phase containing a nonpolar surfactant into an aqueous phase, using ultrasonication. The solvent-free hydrophobic phase that contained tetradecan-1-ol, γ-Fe2O3 nanocrystals, orlistat, and surfactant was dispersed into a warm aqueous surfactant solution, with the formation of small droplets. Then, a pre-cooled aqueous phase was added for rapid cooling and the formation of solid magnetic nanocarriers. Two different nonpolar surfactants, polyethylene glycol dodecyl ether (B4) and our own N1,N1-dimethyl-N2-(tricosan-12-yl)ethane-1,2-diamine (SP11), were investigated for the preparation of MC-B4 and MC-SP11 magnetic nanocarriers, respectively. The nanocarriers formed were of spherical shape, with mean hydrodynamic sizes <160 nm, good colloidal stability, and high drug loading (7.65 wt.%). The MC-B4 nanocarriers showed prolonged drug release, while no drug release was seen for the MC-SP11 nanocarriers over the same time frame. Thus, the selection of a nonpolar surfactant for preparation of magnetic nanocarriers is crucial to enable drug release from nanocarrier.

scholarly journals Cell-Penetrating Peptide and Transferrin Co-Modified Liposomes for Targeted Therapy of Glioma

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3540 ◽  
Author(s):  
Xi Wang ◽  
Yarong Zhao ◽  
Shiyan Dong ◽  
Robert J. Lee ◽  
Dongsheng Yang ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Therapeutic Agent ◽  
Spherical Shape ◽  
Cell Penetrating Peptides ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Great Promise ◽  
Uniform Size ◽  
Cell Penetrating ◽  
Low Toxicity

Glioma is one of the most aggressive and common malignant brain tumors. Due to the presence of the blood-brain barrier (BBB), the effectiveness of therapeutics is greatly affected. In this work, to develop an efficient anti-glioma drug with targeting and which was able to cross the BBB, cell-penetrating peptides (R8) and transferrin co-modified doxorubicin (DOX)-loaded liposomes (Tf-LPs) were prepared. Tf-LPs possessed a spherical shape and uniform size with 128.64 nm and their ξ-potential was 6.81 mV. Tf-LPs were found to be stable in serum within 48 h. Uptake of Tf-LPs in both U87 and GL261 cells was analyzed by confocal laser scanning microscopy and by flow cytometry. Tf-LPs were efficiently taken up by both U87 and GL261 cells. Moreover, Tf-LPs exhibited sustained-release. The cumulative release of DOX from Tf-LPs reached ~50.0% and showed excellent anti-glioma efficacy. Histology of major organs, including brain, heart, liver, spleen, lungs and kidney, and the bodyweight of mice, all indicated low toxicity of Tf-LPs. In conclusion, Tf-LPs showed great promise as an anti-glioma therapeutic agent.

Preparation and Particle Size Characterization of Cu Nanoparticles Prepared by Anodic Arc Plasma

2010 ◽  
Vol 92 ◽  
pp. 163-169
Author(s):  
Hong Xia Qiao ◽  
Zhi Qiang Wei ◽  
Ming Ru Zhou ◽  
Zhong Mao He
Keyword(s):  
Crystal Structure ◽  
Particle Size ◽  
Specific Surface ◽  
Size Distribution ◽  
Surface Area ◽  
Specific Surface Area ◽  
Average Particle Size ◽  
Spherical Shape ◽  
Average Particle ◽  
Uniform Size

Copper nanoparticles were successfully prepared in large scales by means of anodic arc discharging plasma method in inert atmosphere. The particle size, specific surface area, crystal structure and morphology of the samples were characterized by X-ray diffraction (XRD), BET equation, transmission electron microscopy (TEM) and the corresponding selected area electron diffraction (SAED). The experiment results indicate that the crystal structure of the samples is fcc structure as same as that of the bulk materials. The specific surface area is is 11 m2/g, with the particle size distribution ranging from 30 to 90 nm, the average particle size about 67nm obtained from TEM and confirmed from XRD and BET results. The nanoparticles have uniform size, higher purity, narrow size distribution and spherical shape can be prepared by this convenient and effective method.

scholarly journals Photoregulation of Gold Nanoparticles Stabilized in a Diacetylenic Nanocapsule

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Saowalak Somjid ◽  
Apiwat Chompoosor ◽  
Somdej Kanokmedhakul ◽  
Saowapak Teerasong
Keyword(s):  
Gold Nanoparticles ◽  
Uv Irradiation ◽  
Irradiation Time ◽  
Spherical Shape ◽  
Average Diameter ◽  
Drug Carriers ◽  
Hydrophobic Core ◽  
Triggered Release ◽  
Hydrophobic Molecule ◽  
Positively Charged Gold Nanoparticle

The results of releasing a drug in a burst are unpredictable and one of the inherent drawbacks of using nanocarriers. Here, photoresponsive cationic gold nanoparticles to stabilize diacetylenic nanocapsules enabling photoregulated release of payloads are reported. The fabrication of these nanocapsules relied on an electrostatic interaction of a negatively charged diacetylenic core and a positively charged gold nanoparticle shell. Gold nanoparticles with photoresponsive ligands on their surfaces act as both hydrophobic core stabilizers and gatekeepers of the nanocapsules, while their polydiacetylene cores serve as hydrophobic drug carriers that can be tuned using UV irradiation. The morphology of nanocapsules was analyzed using TEM and dynamic light scattering. The resultant nanocapsules had a spherical shape with an average diameter of 152.9 ± 6.7 nm. Upon UV irradiation, the nanocapsules lost their integrity and an encapsulated model compound was released through diffusion. The release of a hydrophobic molecule was irradiation time dependent and thereby controllable. This light-triggered release provides an alternative strategy for controlled drug delivery.

scholarly journals A Microwave-Assisted Synthesis of Zinc Oxide Nanocrystals Finely Tuned for Biological Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 212 ◽  
Author(s):  
Nadia Garino ◽  
Tania Limongi ◽  
Bianca Dumontel ◽  
Marta Canta ◽  
Luisa Racca ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Colloidal Stability ◽  
Cell Uptake ◽  
Biological Behavior ◽  
Microwave Assisted Synthesis ◽  
Biological Applications ◽  
Uniform Size ◽  
Chemical Surface ◽  
Microwave Assisted ◽  
Synthesis Procedure

Herein we report a novel, easy, fast and reliable microwave-assisted synthesis procedure for the preparation of colloidal zinc oxide nanocrystals (ZnO NCs) optimized for biological applications. ZnO NCs are also prepared by a conventional solvo-thermal approach and the properties of the two families of NCs are compared and discussed. All of the NCs are fully characterized in terms of morphological analysis, crystalline structure, chemical composition and optical properties, both as pristine nanomaterials or after amino-propyl group functionalization. Compared to the conventional approach, the novel microwave-derived ZnO NCs demonstrate outstanding colloidal stability in ethanol and water with long shelf-life. Furthermore, together with their more uniform size, shape and chemical surface properties, this long-term colloidal stability also contributes to the highly reproducible data in terms of biocompatibility. Actually, a significantly different biological behavior of the microwave-synthesized ZnO NCs is reported with respect to NCs prepared by the conventional synthesis procedure. In particular, consistent cytotoxicity and highly reproducible cell uptake toward KB cancer cells are measured with the use of microwave-synthesized ZnO NCs, in contrast to the non-reproducible and scattered data obtained with the conventionally-synthesized ones. Thus, we demonstrate how the synthetic route and, as a consequence, the control over all the nanomaterial properties are prominent points to be considered when dealing with the biological world for the achievement of reproducible and reliable results, and how the use of commercially-available and under-characterized nanomaterials should be discouraged in this view.

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