scholarly journals Erratum: Hong, E., et al. Toll-Like Receptor-Mediated Recognition of Nucleic Acid Nanoparticles (NANPs) in Human Primary Blood Cells. Molecules 2019, 24, 1094

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3852
Author(s):  
Enping Hong ◽  
Justin R. Halman ◽  
Ankit Shah ◽  
Edward Cedrone ◽  
Nguyen Truong ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Blood Cells ◽  
Editorial Office ◽  
Toll Like Receptor

The Molecules Editorial Office wishes to make the following erratum to this paper [...]

scholarly journals Bat Red Blood Cells express Nucleic Acid Sensing Receptors and bind RNA and DNA

2022 ◽  
Author(s):  
LK Metthew Lam ◽  
Jane Dobkin ◽  
Kaitlyn A. Eckart ◽  
Ian Gereg ◽  
Andrew DiSalvo ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Red Blood Cells ◽  
Immune Function ◽  
Blood Cells ◽  
Toll Like Receptors ◽  
Cpg Dna ◽  
Human Rbcs ◽  
Insight Into ◽  
Rna And Dna

Red blood cells (RBCs) demonstrate immunomodulatory capabilities through the expression of nucleic acid sensors. Little is known about bat RBCs, and no studies have examined the immune function of bat erythrocytes. Here we show that bat RBCs express the nucleic acid-sensing Toll-like receptors TLR7 and TLR9 and bind the nucleic acid ligands, single-stranded RNA, and CpG DNA. Collectively, these data suggest that, like human RBCs, bat erythrocytes possess immune function and may be reservoirs for nucleic acids. These findings provide unique insight into bat immunity and may uncover potential mechanisms by which virulent pathogens in humans are concealed in bats.

scholarly journals Nucleic acid agonists for Toll-like receptor 7 are defined by the presence of uridine ribonucleotides

2006 ◽  
Vol 36 (12) ◽  
pp. 3256-3267 ◽  
Author(s):  
Sandra S. Diebold ◽  
Catherine Massacrier ◽  
Shizuo Akira ◽  
Carine Paturel ◽  
Yannis Morel ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Toll Like Receptor

scholarly journals Pardaxin Promoted Differentiation and Maturation of Leukemic Cells via Regulating TLR2/MyD88 Signal against Cell Proliferation

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Wu-Ching Uen ◽  
Chen-Yen Choong ◽  
Chen-Jei Tai ◽  
Cheng-Jeng Tai
Keyword(s):  
Blood Cells ◽  
Superoxide Anion ◽  
Therapeutic Potential ◽  
Leukemic Cells ◽  
U937 Cells ◽  
Toll Like Receptor ◽  
Marine Fish Species ◽  
Superoxide Anion Production ◽  
Anion Production ◽  
Myd88 Protein

Objective. Leukemia is a cancer of the blood cells. Leukemic THP-1 and U937 cells were used in this study as monocytic effectors cells for proliferation responses and macrophage-like cells induction in leukemia. Pardaxin is an antimicrobial peptide isolated from the marine fish species.Methods. After treatment for 5 days, pardaxin significantly suppressed cell viability and arrested cell cycle at G0/G1 phase in leukemic cells which were evaluated.Results. Pardaxin also induced cell differentiation and maturation of THP-1 and U937 cells into macrophage-like cells with phagocytotic ability. Moreover, pardaxin elevated the expression of MyD88 but not toll-like receptor (TLR)-2 in both leukemic cells. TLR-2 blocking peptide was used to confirm that pardaxin attenuated phagocytotic ability and superoxide anion production in leukemic cells via activating MyD88 protein.Conclusions. These findings suggested that pardaxin has a therapeutic potential for leukemia.

Cytokine production by whole blood cells of the reconvalescents of community-acquired pneumonia under the influence of low-intensity microwave irradiation

10.12737/5025 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Никифоров ◽  
V. Nikiforov ◽  
Терехов ◽  
I. Terekhov ◽  
Хадарцев ◽  
...  
Keyword(s):  
Microwave Radiation ◽  
Whole Blood ◽  
Blood Cells ◽  
Cytokine Production ◽  
Cell Activity ◽  
Community Acquired Pneumonia ◽  
Tnf Alpha ◽  
Toll Like Receptor ◽  
Low Intensity ◽  
Whole Blood Cells

The study discusses the effect on the functional state of the whole blood cells of low-intensity microwave radiation. On the model of intercellular interactions in the conditions of spontaneous cell activity and by stimulating cells complex mitogen, the influence of microwave radiation 1000 MHz on production by whole blood cells interleukins: IL-1β, 2, 6, 8, 10, 12, 13, 17A, TNF-alpha, IFN-g, RAIL-1, and soluble forms of toll-like receptor 1, 2, 4, 6 types was studied. The research results show that a single 45 minutes of exposure of microwave radiation of 0.05 PPM microwatt/cm2 has the ability to enhance mitogen-stimulated production of RAIL-1 by 38,7% (p=0.03), to reduce the induced production of IL-1β by 26,3% (p=0,037), IL-8 by 56,2% (p=0.022) and to increase oppressed segment complex mitogen production of IL-10 by 27,8% (p=0,041). The microwave radiation increased spontaneous and mitogen-stimulated expression of TLR, especially TLR1 during the initial low level.

scholarly journals Human macrophages utilize a wide range of pathogen recognition receptors to recognize Legionella pneumophila, including Toll-Like Receptor 4 engaging Legionella lipopolysaccharide and the Toll-like Receptor 3 nucleic-acid sensor

2021 ◽  
Vol 17 (7) ◽  
pp. e1009781
Author(s):  
Lubov S. Grigoryeva ◽  
Nicholas P. Cianciotto
Keyword(s):  
Nucleic Acid ◽  
Legionella Pneumophila ◽  
Critical Role ◽  
U937 Cells ◽  
Toll Like Receptor ◽  
Lectin Receptors ◽  
Macrophage Response ◽  
Human Macrophages ◽  
Wide Range ◽  
Pathogen Recognition Receptors

Cytokines made by macrophages play a critical role in determining the course of Legionella pneumophila infection. Prior murine-based modeling indicated that this cytokine response is initiated upon recognition of L. pneumophila by a subset of Toll-like receptors, namely TLR2, TLR5, and TLR9. Through the use of shRNA/siRNA knockdowns and subsequently CRISPR/Cas9 knockouts (KO), we determined that TRIF, an adaptor downstream of endosomal TLR3 and TLR4, is required for full cytokine secretion by human primary and cell-line macrophages. By characterizing a further set of TLR KO’s in human U937 cells, we discerned that, contrary to the viewpoint garnered from murine-based studies, TLR3 and TLR4 (along with TLR2 and TLR5) are in fact vital to the macrophage response in the early stages of L. pneumophila infection. This conclusion was bolstered by showing that i) chemical inhibitors of TLR3 and TLR4 dampen the cytokine output of primary human macrophages and ii) transfection of TLR3 and TLR4 into HEK cells conferred an ability to sense L. pneumophila. TLR3- and TLR4-dependent cytokines promoted migration of human HL-60 neutrophils across an epithelial layer, pointing to the biological importance for the newfound signaling pathway. The response of U937 cells to L. pneumophila LPS was dependent upon TLR4, a further contradiction to murine-based studies, which had concluded that TLR2 is the receptor for Legionella LPS. Given the role of TLR3 in sensing nucleic acid (i.e., dsRNA), we utilized newly-made KO U937 cells to document that DNA-sensing by cGAS-STING and DNA-PK are also needed for the response of human macrophages to L. pneumophila. Given the lack of attention given them in the bacterial field, C-type lectin receptors were similarly examined; but, they were not required. Overall, this study arguably represents the most extensive, single-characterization of Legionella-recognition receptors within human macrophages.

scholarly journals Toll-Like Receptor-Mediated Recognition of Nucleic Acid Nanoparticles (NANPs) in Human Primary Blood Cells

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1094 ◽  
Author(s):  
Enping Hong ◽  
Justin Halman ◽  
Ankit Shah ◽  
Edward Cedrone ◽  
Nguyen Truong ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Rational Design ◽  
Size Composition ◽  
Immune Recognition ◽  
Toll Like Receptor ◽  
Structure Activity ◽  
Infusion Reactions ◽  
Major Route ◽  
Comprehensive Study

Infusion reactions (IRs) create a translational hurdle for many novel therapeutics, including those utilizing nanotechnology. Nucleic acid nanoparticles (NANPs) are a novel class of therapeutics prepared by rational design of relatively short oligonucleotides to self-assemble into various programmable geometric shapes. While cytokine storm, a common type of IR, has halted clinical development of several therapeutic oligonucleotides, NANP technologies hold tremendous potential to bring these reactions under control by tuning the particle’s physicochemical properties to the desired type and magnitude of the immune response. Recently, we reported the very first comprehensive study of the structure–activity relationship between NANPs’ shape, size, composition, and their immunorecognition in human cells, and identified the phagolysosomal pathway as the major route for the NANPs’ uptake and subsequent immunostimulation. Here, we explore the molecular mechanism of NANPs’ recognition by primary immune cells, and particularly the contributing role of the Toll-like receptors. Our current study expands the understanding of the immune recognition of engineered nucleic acid-based therapeutics and contributes to the improvement of the nanomedicine safety profile.

Aberrant Toll-like receptor 2 promoter methylation in blood cells from patients with pulmonary tuberculosis

2014 ◽  
Vol 69 (6) ◽  
pp. 546-557 ◽  
Author(s):  
Yung-Che Chen ◽  
Chang-Chun Hsiao ◽  
Chung-Jen Chen ◽  
Tung-Ying Chao ◽  
Sum-Yee Leung ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Promoter Methylation ◽  
Blood Cells ◽  
Toll Like Receptor ◽  
Toll Like Receptor 2

Sequence analysis confirmation of transfusion-transmitted hepatitis C by red blood cells that tested negative by minipool hepatitis C virus nucleic acid testing

Transfusion ◽  
2007 ◽  
Vol 47 (6) ◽  
pp. 1102-1104 ◽  
Author(s):  
Piotr Grabarczyk ◽  
Agnieszka Gronowska ◽  
Ewa Brojer ◽  
Magdalena Letowska ◽  
Piotr Radziwon
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nucleic Acid ◽  
Sequence Analysis ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Nucleic Acid Testing
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