scholarly journals Surface Display of Antigen Protein VP8* of Porcine Rotavirus on Bacillus Subtilis Spores Using CotB as a Fusion Partner

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3793 ◽  
Author(s):  
Wanqiang Li ◽  
Jie Feng ◽  
Jiajun Li ◽  
Jianzhen Li ◽  
Zhenhua Wang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Recombinant Strain ◽  
Shuttle Vector ◽  
Surface Display ◽  
Mucosal Vaccine ◽  
Viral Gastroenteritis ◽  
Fusion Partner ◽  
Spore Surface ◽  
Effective Measure ◽  
Porcine Rotavirus

Porcine rotavirus is a major cause of acute viral gastroenteritis in suckling piglets, and vaccination is considered to be an effective measure to control these infections. The development of a live mucosal vaccine using Bacillus subtilis spores as an antigen delivery vehicle is a convenient and attractive vaccination strategy against porcine rotavirus. In this study, a shuttle vector was constructed for the spore surface display of the spike protein VP8* from porcine rotavirus (the genotype was G5P[7]). A successful display of the CotB-VP8* fusion protein on the spore surface was confirmed by Western blot and immunofluorescence microscopy analysis. The capacity for immune response generated after immunization with the recombinant strain was evaluated in a mouse model. The intestinal fecal IgA and serum IgG were detected by enzyme-linked-immunosorbent serologic assay (ELISA). Importantly, recombinant strain spores could elicit strong specific mucosal and humoral immune responses. These encouraging results suggest that recombinant B. subtilis BV could provide a strategy for a potential novel application approach to the development of a new and safe mucosal subunit vaccine against porcine rotavirus.

Bacillus subtilis Spore Surface Display of Haloalkane Dehalogenase DhaA

2019 ◽  
Vol 76 (10) ◽  
pp. 1161-1167 ◽  
Author(s):  
Fuli Wang ◽  
Tianyu Song ◽  
Hui Jiang ◽  
Chengxin Pei ◽  
Qibin Huang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Surface Display ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Haloalkane Dehalogenase ◽  
Bacillus Subtilis Spore ◽  
Spore Surface Display

scholarly journals Bacillus subtilis spore surface display of photodecarboxylase for the transformation of lipids to hydrocarbons

2020 ◽  
Author(s):  
Marianna Karava ◽  
Peter Gockel ◽  
Johannes Kabisch
Keyword(s):  
Bacillus Subtilis ◽  
Immobilized Lipase ◽  
Surface Display ◽  
Immobilized Enzymes ◽  
Spore Surface ◽  
Oleaginous Yeasts ◽  
Biotechnological Production ◽  
One Pot ◽  
Bacillus Subtilis Spore ◽  
3D Printed

AbstractThe display of enzymes on the surface of spores allows the rapid and very simple biotechnological production of immobilized enzymes. Here we describe the development of a Bacillus subtilis spore display platform and its application to produce hydrocarbons from lipids obtained from the oleaginous yeasts Yarrowia lipolytica, Cutaneotrichosporon oleaginosus as well as olive oil.Lipid hydrolysis was examined in a bienzymatic one-pot cascade using a commercially immobilized lipase (RO lipase) as well as spores with and without additional heterologous lipase expression. Decarboxylation of the released fatty acids was achieved displaying a photodecarboxylase (CvFAP) on the spore surface. Differences in composition of the formed hydrocarbons were observed depending on the lipids source. Using 3D printed lighting equipment titers of up to 64.0 ± 5.6 mg/L hydrocarbons were produced.

scholarly journals Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis

2017 ◽  
Vol 27 (3) ◽  
pp. 159-167 ◽  
Author(s):  
Huayou Chen ◽  
Jawad Ullah ◽  
Jinru Jia
Keyword(s):  
Bacillus Subtilis ◽  
Industrial Development ◽  
Vaccine Development ◽  
Low Cost ◽  
Surface Display ◽  
Cell Surface Display ◽  
Coat Proteins ◽  
Spore Surface ◽  
Spore Surface Display ◽  
Alkaline Tolerance

Spore surface display is the most desirable with enhanced effects, low cost, less time consuming and the most promising technology for environmental, medical, and industrial development. Spores have various applications in industry due to their ability to survive in harsh industrial processes including heat resistance, alkaline tolerance, chemical tolerance, easy recovery, and reusability. Yeast and bacteria, including gram-positive and -negative, are the most frequently used organisms for the display of various proteins (eukaryotic and prokaryotic), but unlike spores, they can rupture easily due to nutritive properties, susceptibility to heat, pH, and chemicals. Hence, spores are the best choice to avoid these problems, and they have various applications over nonspore formers due to amenability for laboratory purposes. Various strains of <i>Clostridium</i> and <i>Bacillus</i> are spore formers, but the most suitable choice for display is <i>Bacillus subtilis</i> because, according to the WHO, it is safe to humans and considered as “GRAS” (generally recognized as safe). This review focuses on the application of spore surface display towards industries, vaccine development, the environment, and peptide library construction, with cell surface display for enhanced protein expression and high enzymatic activity. Different vectors, coat proteins, and statistical analyses can be used for linker selection to obtain greater expression and high activity of the displayed protein.

scholarly journals Genetic Construction of Bacillus subtilis Recombinant Strain, Producing Anthrax Protective Antigen

2018 ◽  
Vol 2 (2) ◽  
pp. 51-60
Keyword(s):  
Bacillus Subtilis ◽  
Recombinant Strain ◽  
Protective Antigen ◽  
Shuttle Vector ◽  
Subtilis Strain ◽  
Anthrax Vaccine ◽  
Anthrax Protective Antigen ◽  
Recombinant Bacillus Subtilis ◽  
New Generation ◽  
Anthrax Vaccines

Anthrax is a serious infectious disease with high mortality. The epidemiological security depends on the vaccination of susceptible animals and population at risk. But many of the existing anthrax vaccine strains possess low levels of protective antigen production and high reactogenicity. One of the most promising trends in production of new generation of vaccines is the cloning of particular determinants of immunogenicity of anthrax microbe for the creation of highly effective producers of Bacillus anthracis protective antigen. The aim of the article is to present the results of the study on the construction of recombinant Bacillus subtilis strain, producing B.anthracis protective antigen, promising for use in chemical anthrax vaccines technology. The pHT43PA plasmid containing the gene pag, providing the synthesis of protective antigen of the anthrax microbe and functioning stably in the cells of the recombinant strain Amy21(pHT43РА) of B. subtilis, was constructed on the basis of the shuttle vector pHT43. It is found out during the research, that the microbial cells of the recombinant strain Amy21(pHT43РА) of B. subtilis provide the production of immunologically active protective antigen in quantities, not inferior than anthrax vaccine strains. These data, as well as safety and simplicity of В. subtilis make it possible to continue the research of this recombinant strain as a producer of anthrax protective antigen, promising for use in vaccines production

scholarly journals ThespsGene Products Affect the Germination, Hydrophobicity, and Protein Adsorption of Bacillus subtilis Spores

2014 ◽  
Vol 80 (23) ◽  
pp. 7293-7302 ◽  
Author(s):  
Giuseppina Cangiano ◽  
Teja Sirec ◽  
Cristina Panarella ◽  
Rachele Isticato ◽  
Loredana Baccigalupi ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Experimental Validation ◽  
Surface Display ◽  
Deep Understanding ◽  
Transcriptional Analysis ◽  
Functional Study ◽  
Spore Surface ◽  
Wild Type ◽  
Content Type ◽  
Hydrophobic Force

ABSTRACTThe multilayered surface of theBacillus subtilisspore is composed of proteins and glycans. While over 70 different proteins have been identified as surface components, carbohydrates associated with the spore surface have not been characterized in detail yet. Bioinformatic data suggest that the 11 products of thespsoperon are involved in the synthesis of polysaccharides present on the spore surface, but an experimental validation is available only for the four distal genes of the operon. Here, we report a transcriptional analysis of thespsoperon and a functional study performed by constructing and analyzing two null mutants lacking either all or only the promoter-proximal gene of the operon. Our results show that bothspsmutant spores apparently have normal coat and crust but have a small germination defect and are more hydrophobic than wild-type spores. We also show that spores lacking all Sps proteins are highly adhesive and form extensive clumps. In addition,spsmutant spores have an increased efficiency in adsorbing a heterologous enzyme, suggesting that hydrophobic force is a major determinant of spore adsorption and indicating that a deep understanding of the surface properties of the spore is essential for its full development as a surface display platform.

scholarly journals Bacillus subtilis Spore Surface Display Technology: A Review of Its Development and Applications

2019 ◽  
Vol 29 (2) ◽  
pp. 179-190 ◽  
Author(s):  
Zhang Guoyan ◽  
An Yingfeng ◽  
Hossain M Zabed ◽  
Guo Qi ◽  
Miaomiao Yang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Surface Display ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Bacillus Subtilis Spore ◽  
Spore Surface Display ◽  
Display Technology

scholarly journals A system of vectors for Bacillus subtilis spore surface display

2014 ◽  
Vol 13 (1) ◽  
pp. 30 ◽  
Author(s):  
Adam Iwanicki ◽  
Iwona Piątek ◽  
Małgorzata Stasiłojć ◽  
Anna Grela ◽  
Tomasz Łęga ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Surface Display ◽  
Spore Surface ◽  
Subtilis Spore ◽  
Bacillus Subtilis Spore ◽  
Spore Surface Display
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