scholarly journals In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3353 ◽  
Author(s):  
Oya Ustuner ◽  
Ceren Anlas ◽  
Tulay Bakirel ◽  
Fulya Ustun-Alkan ◽  
Belgi Diren Sigirci ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Folk Medicine ◽  
Traditional Use ◽  
Necrosis Factor Alpha ◽  
Treatment Protocols ◽  
Fibroblast Migration ◽  
Anti Inflammatory ◽  
Treatment Of Wounds

Thymus sipyleus Boiss. subsp. rosulans (Borbas) Jalas (TS) is a commonly used plant in the treatment of various complaints, including skin wounds in Turkish folk medicine. Despite the widespread traditional use of TS, there is not any scientific report confirming the effectiveness of this plant on the healing process. This research aimed to investigate the effects of different extracts obtained from TS on biological events during wound healing, on a cellular basis. In this context, proliferative activities of the extracts, as well as the effects on wound closure and hydroxyproline synthesis, were determined. In addition to wound healing properties, the antioxidant, antibacterial and anti-inflammatory activities of the extracts were evaluated. Decoction (D) and infusion (I) extracts contained the highest amount of phenolic content and showed the most potent activity against DPPH radical. All extracts exhibited complete protection against the damage induced by hydrogen peroxide (H2O2) by increasing cell viability compared to only H2O2-treated groups, both in co-treatment and pre-treatment protocols. None of the extracts exhibited cytotoxic activity, and most of the extracts from the TS stimulated fibroblast proliferation and migration. All TS extracts exert anti-inflammatory activity by suppressing the overproduction of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO). The most pronounced activity on hydroxyproline synthesis was observed in D extract. In summary, it was observed that TS extracts can promote the healing process by enhancing fibroblast migration, proliferation and collagen synthesis as well as suppressing pro-inflammatory cytokines. The obtained data in this work support the traditional use of TS as a valuable plant-based compound for the treatment of wounds.

scholarly journals Wound Healing Activity of the Essential Oil of Bursera morelensis, in Mice

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1795
Author(s):  
Judith Salas-Oropeza ◽  
Manuel Jimenez-Estrada ◽  
Armando Perez-Torres ◽  
Andres Eliu Castell-Rodriguez ◽  
Rodolfo Becerril-Millan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Essential Oil ◽  
Cell Viability ◽  
Murine Model ◽  
Biological Activities ◽  
Healing Process ◽  
In Vitro Tests ◽  
Wound Healing Process ◽  
Fibroblast Migration

Bursera morelensis is used in Mexican folk medicine to treat wounds on the skin. It is an endemic tree known as “aceitillo”, and the antibacterial and antifungal activity of its essential oil has been verified; it also acts as an anti-inflammatory. All of these reported biological activities make the essential oil of B. morelensis a candidate to accelerate the wound-healing process. The objective was to determine the wound-healing properties of B. morelensis’ essential oil on a murine model. The essential oil was obtained by hydro-distillation, and the chemical analysis was performed by gas chromatography-mass spectrometry (GC-MS). In the murine model, wound-healing efficacy (WHE) and wound contraction (WC) were evaluated. Cytotoxic activity was evaluated in vitro using peritoneal macrophages from BALB/c mice. The results showed that 18 terpenoid-type compounds were identified in the essential oil. The essential oil had remarkable WHE regardless of the dose and accelerated WC and was not cytotoxic. In vitro tests with fibroblasts showed that cell viability was dose-dependent; by adding 1 mg/mL of essential oil (EO) to the culture medium, cell viability decreased below 80%, while, at doses of 0.1 and 0.01 mg/mL, it remained around 90%; thus, EO did not intervene in fibroblast proliferation, but it did influence fibroblast migration when wound-like was done in monolayer cultures. The results of this study demonstrated that the essential oil was a pro-wound-healing agent because it had good healing effectiveness with scars with good tensile strength and accelerated repair. The probable mechanism of action of the EO of B. morelensis, during the healing process, is the promotion of the migration of fibroblasts to the site of the wound, making them active in the production of collagen and promoting the remodeling of this collagen.

scholarly journals Evaluation of in vitro fibroblast migration by electrospun triple-layered PU-CA/gelatin.PRGF/PU-CA scaffold using an AAVS1 targeted EGFP reporter cell line

Bioimpacts ◽  
2021 ◽  
Author(s):  
Forough Shams ◽  
Hamideh Moravvej ◽  
Simzar Hosseinzadeh ◽  
Bahram Kazemi ◽  
Masoumrh Rajabibazl ◽  
...  
Keyword(s):  
Wound Healing ◽  
Real Time ◽  
Reporter Gene ◽  
Healing Process ◽  
Functional Evaluation ◽  
Fibroblast Cells ◽  
Fibroblast Migration ◽  
Egfp Reporter ◽  
And Migration

Introduction: Migration of fibroblast cells in wound areas is a critical aspect of the wound healing process. Employment of enhanced green fluorescent protein (EGFP) labeled fibroblast cells facilitates real-time monitoring and functional evaluation of these cells in both in vitro and in vivo settings. Plasma rich in growth factor (PRGF) is a potent accelerator of wound healing; therefore, in this study, a novel method to fabricate an electrospun bioactive scaffold containing PRGF was employed to induce in vitro cell proliferation and migration. Methods: First, the EGFP reporter gene was integrated into the AAVS1 locus of fibroblast cells using CRISPR/Cas9 system. Then, PRGF was obtained from platelet-rich plasma, and a multi-layered scaffold was fabricated using polyurethane-cellulose acetate (PU-CA) fibers as the outer layers and PRGF-containing gelatin fibers were located in the internal layer like a central strip. Scanning electron microscopy (SEM), tensile, water contact angle, and FTIR tests were performed to assess the characteristics of the scaffolds. The EGFP targeted cells were cultured on scaffolds with or without PRGF to investigate their viability, toxicity, and migration pattern in response to the release profile. Results: Fluorescence images showed that the number of migrating cells on scaffold containing PRGF was more significant than PU-CA scaffold up to day 6. Increased expression of SGPL1, DDR2, and VEGF genes was also observed on the scaffold containing PRGF compared to PU-CA using real-time polymerase chain reaction (PCR) analysis with around 3-, 2-, and 2-fold enhancement, respectively. Conclusion: The current scaffold provides the appropriate template for cell attachment and migration. In addition, the present results highlight the potential of reporter gene targeting for the in vitro analysis of biological processes such as migration.

scholarly journals In Vitro and In Vivo Wound Healing and Anti-Inflammatory Activities of Babassu Oil (Attalea speciosa Mart. Ex Spreng., Arecaceae)

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
José Alex A. Santos ◽  
José Wellinton da Silva ◽  
Simone Maria dos Santos ◽  
Maria de Fátima Rodrigues ◽  
Camila Joyce A. Silva ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Palm Tree ◽  
Amazon Forest ◽  
Myeloperoxidase Activity ◽  
Skin Wounds ◽  
Babassu Oil ◽  
Anti Inflammatory

Babassu (Attalea speciosa Mart. ex Spreng., Arecaceae) is a palm tree endemic to Brazil and found mainly in the borders of Amazon forest, where the harvesting of its fruits is an important source of income for more than 300,000 people. Among the communities of coconut breakers women, babassu oil is used in culinary, as fuel, and mostly as medicinal oil for the treatment of skin wounds and inflammation. This study aimed to evaluate in vitro and in vivo the wound healing effects of babassu oil. In vitro, babassu oil increased the migration of L929 fibroblasts, inhibited the production of nitric oxide by LPS-stimulated peritoneal macrophages, and increased the levels of INF-γ and IL-6 cytokines production. In vivo, babassu oil accelerated the healing process in a full-thickness splinted wound model, by an increase in the fibroblasts number, blood vessels, and collagen deposition in the wounds. The babassu oil also increased the recruitment of inflammatory cells into the wound site and showed an anti-inflammatory effect in a chronic ear edema model, reducing ear thickness, epidermal hyperplasia, and myeloperoxidase activity. Thus, these data corroborate the use of babassu oil in folk medicine as a remedy to treat skin wounds.

scholarly journals Wound Healing Activity of α-Pinene and α-Phellandrene

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2488
Author(s):  
Judith Salas-Oropeza ◽  
Manuel Jimenez-Estrada ◽  
Armando Perez-Torres ◽  
Andres Eliu Castell-Rodriguez ◽  
Rodolfo Becerril-Millan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Folk Medicine ◽  
Fibroblast Cell ◽  
In Vitro Tests ◽  
Low Concentrations ◽  
In Vivo Tests ◽  
Wound Healing Activity

Bursera morelensis is used in Mexican folk medicine to treat wounds on the skin. Recently, it was shown that the essential oil (EO) of B. morelensis has wound healing activity, accelerating cutaneous wound closure and generating scars with good tensile strength. α-pinene (PIN) and α-phellandrene (FEL) are terpenes that have been found in this EO, and it has been shown in different studies that both have anti-inflammatory activity. The aim of this study was to determine the wound healing activity of these two terpenes. The results of in vitro tests demonstrate that PIN and FEL are not cytotoxic at low concentrations and that they do not stimulate fibroblast cell proliferation. In vivo tests showed that the terpenes produce stress-resistant scars and accelerate wound contraction, due to collagen deposition from the early stages, in wounds treated with both terpenes. Therefore, we conclude that both α-pinene and α-phellandrene promote the healing process; this confirms the healing activity of the EO of B. morelensis, since having these terpenes as part of its chemical composition explains part of its demonstrated activity.

Angiostatin is a novel anti-inflammatory factor by inhibiting leukocyte recruitment

Blood ◽  
2005 ◽  
Vol 105 (3) ◽  
pp. 1036-1043 ◽  
Author(s):  
Triantafyllos Chavakis ◽  
Athanasios Athanasopoulos ◽  
Joong-Sup Rhee ◽  
Valeria Orlova ◽  
Thomas Schmidt-Wöll ◽  
...  
Keyword(s):  
Wound Healing ◽  
Vascular Injury ◽  
Healing Process ◽  
Specific Interaction ◽  
Angiogenesis Inhibitor ◽  
Terminal Phase ◽  
Inflammatory Factor ◽  
Anti Inflammatory

AbstractAngiogenesis and inflammation are closely related biologic processes in wound healing and the responses to vascular injury as well as in cardiovascular diseases; however, the molecular connections are poorly defined. In particular, it is yet unclear whether endogenous factors can regulate both angiogenesis and inflammation. Here, we show that the endogenous angiogenesis inhibitor, angiostatin (containing kringle domains 1-4 of plasminogen), serves an anti-inflammatory role, since the kringles 1-3 and its kringle 4 directly interact with leukocyte β1- and β2-integrins, respectively. In particular, a specific interaction between kringle 4 and αMβ2-integrin (Mac-1) but not leukocyte function antigen 1 (LFA-1) was identified. Angiostatin thereby inhibited β1- and β2-integrin–mediated adhesion of leukocytes to extracellular matrix proteins and the endothelium as well as their transmigration through the endothelium in vitro. Moreover, angiostatin blocked the peritonitis-induced neutrophil emigration in vivo. In addition, through its interaction with Mac-1, angiostatin reduced activation of the proinflammatory transcription factor nuclear factor κB (NFκB), as well as the NFκB-related expression of tissue factor, a potent initiator of hemostasis following vascular injury. Finally, angiostatin forms were generated in vivo following skin injury/inflammation and were detectable during the following entire period of wound healing peaking at the terminal phase of the healing process. Taken together, over and above inhibition of neovascularization, angiostatin was identified as an antiadhesive/anti-inflammatory substance. These observations could provide the basis for new therapeutic applications of angiostatin to target chronic inflammatory processes in different pathologic situations.

scholarly journals Effect of Unloading Condition on the Healing Process and Effectiveness of Platelet Rich Plasma as a Countermeasure: Study on In Vivo and In Vitro Wound Healing Models

2020 ◽  
Vol 21 (2) ◽  
pp. 407 ◽  
Author(s):  
Francesca Cialdai ◽  
Alessandra Colciago ◽  
Desiré Pantalone ◽  
Angela Maria Rizzo ◽  
Stefania Zava ◽  
...  
Keyword(s):  
Wound Healing ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Physical Factors ◽  
Medical Evacuation ◽  
Fibroblast Migration ◽  
Wound Healing Model ◽  
Healing Model

Wound healing is a very complex process that allows organisms to survive injuries. It is strictly regulated by a number of biochemical and physical factors, mechanical forces included. Studying wound healing in space is interesting for two main reasons: (i) defining tools, procedures, and protocols to manage serious wounds and burns eventually occurring in future long-lasting space exploration missions, without the possibility of timely medical evacuation to Earth; (ii) understanding the role of gravity and mechanical factors in the healing process and scarring, thus contributing to unravelling the mechanisms underlying the switching between perfect regeneration and imperfect repair with scarring. In the study presented here, a new in vivo sutured wound healing model in the leech (Hirudo medicinalis) has been used to evaluate the effect of unloading conditions on the healing process and the effectiveness of platelet rich plasma (PRP) as a countermeasure. The results reveal that microgravity caused a healing delay and structural alterations in the repair tissue, which were prevented by PRP treatment. Moreover, investigating the effects of microgravity and PRP on an in vitro wound healing model, it was found that PRP is able to counteract the microgravity-induced impairment in fibroblast migration to the wound site. This could be one of the mechanisms underlying the effectiveness of PRP in preventing healing impairment in unloading conditions.

Carpesium macrocephalum Attenuates Lipopolysaccharide-Induced Inflammation in Macrophages by Regulating the NF-κB/IκB-α, Akt, and STAT Signaling Pathways

2013 ◽  
Vol 41 (04) ◽  
pp. 927-943 ◽  
Author(s):  
Sushruta Koppula ◽  
Wan-Jae Kim ◽  
Jun Jiang ◽  
Do-Wan Shim ◽  
Na-Hyun Oh ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
In Vivo Studies ◽  
Raw264.7 Cells ◽  
Traditional Use ◽  
Necrosis Factor Alpha ◽  
Proinflammatory Mediators ◽  
Raw264.7 Macrophages ◽  
Anti Inflammatory

Carpesium macrocephalum (CM) Fr. et Sav. (Compositae) has been used in Chinese folk medicine as an analgesic, hemostatic, antipyretic, and to suppress inflammatory conditions. In the present study we aimed to provide scientific evidence for the anti-inflammatory properties of CM extract and evaluate the intrinsic mechanisms involved in both in vitro and in vivo experimental models. In in vitro findings, CM significantly inhibited the LPS-stimulated release of proinflammatory mediators such as nitric oxide, tumor necrosis factor-alpha, prostaglandin E2, and interleukin-6 in RAW264.7 macrophages in a concentration-dependent fashion. The attenuation of inflammatory responses in LPS-activated RAW264.7 cells by CM was closely associated with the suppression of nuclear factor-kappa B (NF-κB) phosphorylation, IκB-α degradation, and phosphorylation of Akt. CM treatment also attenuated the phosphorylation of STAT through TRIF dependent pathways in LPS-activated RAW264.7 cells. In vivo studies revealed that CM extract concentration dependently suppressed the acetic acid-induced vascular permeability in mice. Considering the data obtained regulation of multiple signaling mechanisms involving TRIF and Akt/NF-κB pathways might be responsible for the potent anti-inflammatory action of CM, substantiating its traditional use in inflammatory diseases.

scholarly journals Euryops arabicus Promotes Healing of Excised Wounds in Rat Skin: Emphasis on Its Collagen-Enhancing, Antioxidant, and Anti-Inflammatory Activities

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Ahmed Abdel-Lateff ◽  
Ashraf B. Abdel-Naim ◽  
Walied M. Alarif ◽  
Mardi M. Algandaby ◽  
Najla A. Alburae ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mrna Expression ◽  
Folk Medicine ◽  
Antioxidant Properties ◽  
Skin Wound ◽  
Tnf Α ◽  
The Family ◽  
Anti Inflammatory ◽  
Tgf Β1

Euryops arabicus Steud (E. arabicus) belongs to the family Asteraceae. It has several uses in folk medicine in the Arabian Peninsula. The current study aimed at evaluating the wound healing properties of the E. arabicus extract in rats. Primarily, E. arabicus successfully accelerated cell migration in vitro and it also showed no signs of dermal toxicity. Topical application of E. arabicus extract (5% or 20%) expedited healing of excised skin in rats. Histological examinations indicated that E. arabicus shortened epithelization period, stimulated fibroblast activity, and increased collagen deposition in wound tissues. The plant extract exerted antioxidant activity as evidenced by inhibition of GSH depletion and MDA accumulation and enhanced mRNA expression of Sod1 in wound tissues collected at the end of the experiment. Further, E. arabicus inhibited the rise of TNF-α and IL-1β in the skin wound region. The anti-inflammatory was confirmed by the observed down regulation of Ptgs2, Nos2, IL-6, and NF-κB mRNA expression. In addition, the extract enhanced the expression of TGF-β1 and HIF-1α in wounded skin tissues as indicated immunohistochemically. Conclusively, E. arabicus expedites excision wound healing in rats. Collagen-enhancing, anti-inflammatory, and antioxidant properties mediate the observed wound healing activity. These findings might contribute to our understanding of the ethnobotanical use of E. arabicus in wounds.

scholarly journals Medicinal Plants in the Records of Latvian Folk Medicine and Analysis of Their Practical Applications. Doctoral Thesis

2021 ◽  
Author(s):  
◽  
Inga Sīle ◽  
Keyword(s):  
Medicinal Plants ◽  
Molecular Mechanisms ◽  
Ex Vivo ◽  
Folk Medicine ◽  
European Medicines Agency ◽  
Evidence Based ◽  
Traditional Use ◽  
Practical Applications ◽  
Anti Inflammatory

Medicinal plant knowledge in Europe is rooted in a long history of health traditions. Numerous ethnobotanical studies across Europe have addressed the increasing importance of the traditional use of medicinal plants. Latvia is a country with old folk medicine traditions and an extensive folk knowledge archive. The aim of this thesis was to collect and analyse knowledge about the use of the medicinal plants found in the records of Latvian folk medicine and to search for new ideas regarding the practical applications of these plants. This thesis reveals, for the first time to the international scientific community, the important ethnobotanical information contained in the records of Latvian folk medicine that had not yet been translated into English and are therefore less visible to researchers from all over the world. This thesis provides a list of the used plant species and plant parts, the dosage forms of herbal medicines, the routes of administration, and the disorders treated with medicinal plants mentioned in the records of Latvian folk medicine and used by indigenous people of Latvia in the end of 19th and the beginning of 20th centuries. In total, the thesis includes information on 211 plant taxa, most of which were utilized for the treatment of digestive and respiratory system disorders. To understand whether the information mentioned in the folklore material is relevant today, it was compared with evidence-based information regarding the uses of the listed plant, including official herbal monographs. During this study, it was concluded that only 59 plant taxa mentioned in the studied records of Latvian folk medicine are included in the official monographs of the European Medicines Agency, and most of the plant indications mentioned in the records have not been described in evidence-based monographs. After the systematic analysis of folklore materials, additional information on the molecular mechanisms of the anti-inflammatory activities of Pelargonium sidoides DC. and Prunus padus L. were investigated to confirm their traditional use for the treatment of inflammatory conditions. The obtained results provide evidence that both plant extracts exhibit pronounced in vitro and ex vivo anti-inflammatory activities, supporting their use in Latvian ethnomedicine as effective anti-inflammatory agents. The obtained results are important as they provide ideas for further research related to possibilities regarding the use of plants growing in the territory of Latvia and allow new perspectives to be gained for both national and international ethnobotanical research.

scholarly journals Inhibition of Proinflammatory Enzymes and Attenuation of IL-6 in LPS-Challenged RAW 264.7 Macrophages Substantiates the Ethnomedicinal Use of the Herbal Drug Homalium bhamoense Cubitt & W.W.Sm

2020 ◽  
Vol 21 (7) ◽  
pp. 2421 ◽  
Author(s):  
Rungcharn Suksungworn ◽  
Paula B. Andrade ◽  
Andreia P. Oliveira ◽  
Patrícia Valentão ◽  
Sutsawat Duangsrisai ◽  
...  
Keyword(s):  
Wound Healing ◽  
Bark Extract ◽  
Raw 264.7 ◽  
Necrosis Factor Alpha ◽  
Cutaneous Toxicity ◽  
Raw 264.7 Macrophages ◽  
Dependent Inhibition ◽  
Ic50 Values ◽  
Anti Inflammatory

Commonly used to treat skin injuries in Asia, several Homalium spp. have been found to promote skin regeneration and wound healing. While ethnobotanical surveys report the use of H. bhamoense trunk bark as a wound salve, there are no studies covering bioactive properties. As impaired cutaneous healing is characterized by excessive inflammation, a series of inflammatory mediators involved in wound healing were targeted with a methanol extract obtained from H. bhamoense trunk bark. Results showed concentration-dependent inhibition of hyaluronidase and 5-lipoxygenase upon exposure to the extract, with IC50 values of 396.9 ± 25.7 and 29.0 ± 2.3 µg mL−1, respectively. H. bhamoense trunk bark extract also exerted anti-inflammatory activity by significantly suppressing the overproduction of interleukin 6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages at concentrations ranging from 125 to 1000 µg mL−1, while leading to a biphasic effect on nitric oxide (NO) and tumor necrosis factor alpha (TNF-α) levels. The phenolic profile was elucidated by HPLC-DAD, being characterized by the occurrence of ellagic acid as the main constituent, in addition to a series of methylated derivatives, which might underlie the observed anti-inflammatory effects. Our findings provide in vitro data on anti-inflammatory ability of H. bhamoense trunk bark, disclosing also potential cutaneous toxicity as assessed in HaCaT keratinocytes.

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