scholarly journals Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold Storage

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3292 ◽  
Author(s):  
Shiyuan Fang ◽  
Qianqian Zhou ◽  
Yan Hu ◽  
Feng Liu ◽  
Jun Mei ◽  
...  

The objective of this research was to explore the antimicrobial activity and mechanism of carvacrol against Vibrio Parahemolyticus, Shewanella putrefaciens, Staphylococcus aureus and Pseudomonas fluorescens and evaluate the effect of the addition of carvacrol/β-cyclodextrin emulsions to flaxseed gum (FSG)-sodium alginate (SA) edible films on the preservation of Chinese sea bass (Lateolabrax maculatus) fillets during refrigerated storage. The minimum inhibitory concentration (MIC) of carvacrol against V. parahemolyticus, S. putrefaciens, S. aureus and P. fluorescens were 0.5, 0.5, 0.125, and 0.5 mg/mL, respectively. Alkaline phosphatase activity assay, nucleotide and protein leakage, and scanning electron microscope demonstrated that carvacrol damaged the external structure of the tested bacterial cells causing leakage of cytoplasmic components. At the same time, when FSG-SA films containing carvacrol used as coating agents for Chinese sea bass fillets cold storage, FSG-SA films containing 1.0 or 2.0 mg/mL carvacrol could significantly reduce TVB-N content, K-value, the degree of microbial deterioration and maintain quality of sea bass fillets according to organoleptic evaluation results.

Coatings ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 506
Author(s):  
Jun Mei ◽  
Yong Shen ◽  
Wenru Liu ◽  
Weiqing Lan ◽  
Na Li ◽  
...  

This study developed a biopreservation method for ready-to-eat (RTE) fresh salmon fillets based on the use of bacteriocin EFL4 produced by bacteriocinogenic Enterococcus faecalis L04 previously isolated from Chinese sea bass (Lateolabrax maculatus). Bacteriocin EFL4 has the ability to inhibit the growth of several fish-spoilage bacteria and foodborne pathogens, including Staphylococcus aureus, Escherichia coli, Shewanella putrefaciens, Pseudomonas fluorescens and Listeria monocytogenes, and the minimal inhibitory concentration (MIC) for S. putrefaciens was 0.32 μg/mL. The biopreservation potential of bacteriocin EFL4 for RTE fresh salmon fillets during cold storage at 4 °C was tested for the first time on a laboratory scale. Microbiological and physicochemical properties, as well as organoleptic evaluations, have been done during the biopreservation trials. The results show that RTE fresh salmon fillets treated with 0.64 μg/mL bacteriocin EFL4 could significantly (p < 0.05) reduce the total viable count (TVC), total volatile basic nitrogen (TVB-N), K value and maintain the quality of RTE fresh salmon fillets during 8-day storage on the basis of the organoleptic evaluation results.


Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 871 ◽  
Author(s):  
Qianqian Zhou ◽  
Peiyun Li ◽  
Shiyuan Fang ◽  
Jun Mei ◽  
Jing Xie

The purpose of this research was to explore the fresh keeping effect of modified atmosphere packaging (MAP) with different gas ratios combined with gelatin active coatings containing eugenol on Chinese sea bass stored at −0.9 °C for 36 days. The results showed that MAP3 (60% CO2/10% O2/30% N2), together with gelatin active coatings containing eugenol, could prevent water loss, which maintained high field NMR, MRI, and organoleptic evaluation results. This hurdle technology could also effectively delay the bacterial reproduction, protein degradation, and alkaline accumulation, so it showed the lowest K value, total volatile basic nitrogen, free amino acids, total viable count, Pseudomonas spp., and H2S-producing bacteria, which better maintain the quality of sea bass.


Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2645 ◽  
Author(s):  
Jingsong Ye ◽  
Donghui Ma ◽  
Wen Qin ◽  
Yaowen Liu

Edible films have gradually become a research focus for food packaging materials due to a variety of benefits, including environmental friendliness, good barrier properties, and good carrying capacity. In this experimental study, we used sodium alginate as a film-forming substrate, sodium carboxymethylcellulose as a modifier, and glycerol as a plasticizer, then Lactococcus lactis was added to film solutions to form bacteriostatic films via the tape casting method. With the addition of Lactococcus lactis, the films did not significantly change thickness, while the transparency decreased and a significant increase in red and yellow hues was observed. Meanwhile, the dispersion of bacterial cells in film solutions destroyed intermolecular interactions in the solutions during film formation and increased the volume of voids in the Lactococcus lactis-containing films, thereby slightly decreasing the tensile strength of the films, but significantly increasing water vapor permeability. Moreover, the films with added Lactococcus lactis showed significant bacteriostatic activity against Staphylococcus aureus at 4 °C. In a seven-day bacteriostatic test, the films with Lactococcus lactis added at a level of 1.5 g/100 g resulted in a decrease in the viable cell count of Staphylococcus aureus by at least four logarithmic units. This study of Lactococcus lactis-containing films has provided a new method and strategy for antibacterial preservation of foods.


Coatings ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 489 ◽  
Author(s):  
Zhou ◽  
Li ◽  
Fang ◽  
Liu ◽  
Mei ◽  
...  

This research was to evaluate the effects of gelatin (G) active coating containing eugenol/β-cyclodextrin (βCD) emulsions combined with superchilling (−0.9 °C) on physicochemical, microbiological, and organoleptic properties of Chinese seabass samples during 30 days of storage. Results showed that seabass samples dipped in G-βCD coatings containing 0.15% or 0.3% eugenol combined with superchilling could significantly lower the total volatile basic nitrogen, K value, total viable count, H2S-producing bacteria, Pseudomonas spp. and Psychrophilic counts, and free fatty acids. Further, G-βCD coatings containing eugenol with superchilling (−0.9 °C) were more effective in retarding the water migration by low field NMR and MRI results, maintaining quality of seabass during storage according to organoleptic evaluation results.


LWT ◽  
2021 ◽  
pp. 111521
Author(s):  
Jiali Chen ◽  
Weibin Bai ◽  
Dongbao Cai ◽  
Zhiling Yu ◽  
Baojun Xu

2020 ◽  
Vol 145 ◽  
pp. 124-132 ◽  
Author(s):  
Zineb Mahcene ◽  
Aminata Khelil ◽  
Sara Hasni ◽  
Perihan Kubra Akman ◽  
Fatih Bozkurt ◽  
...  

2018 ◽  
Vol 35 (2) ◽  
pp. 551-557 ◽  
Author(s):  
Wenhao Wang ◽  
Hongbiao Dong ◽  
Yongxu Sun ◽  
Ming Cao ◽  
Yafei Duan ◽  
...  

2021 ◽  
Vol 4 (1) ◽  
pp. 946-954
Author(s):  
Nguyen Van Giang ◽  
Phan Thanh Nghi ◽  
Nguyen Bich Hanh

Spotted sea bass (Lateolabrax maculatus, McClelland, 1844) is a species belonging to the genus Lateolabrax (Lateolabracidae), and is characterized by the many distinct black spots on the bodies of mature fish. For the first time, six specimens have been recorded in Quang Ninh province. The specimens were collected from Luon Cave in Bai Tu Long National Park in May-June 2019. The new specimens collected from Quang Ninh, Vietnam are different from the recorded specimens from Japan, Korea, Taiwan, and China in terms of lateral line scales (LLS) as the samples collected in Quang Ninh, Vietnam have 94-97 scales and the samples collected from the other countries have 66-82 scales. Phylogenetic analysis of the 16S rDNA sequence confirmed that all of the samples collected in Quang Ninh, Vietnam belonged to the species of spotted sea bass.


Author(s):  
Baohua Chen ◽  
Zhixiong Zhou ◽  
Yue Shi ◽  
Jie Gong ◽  
Chengyu Li ◽  
...  

Temperature is an important climatic factor that shapes the distribution of eurythermal species. Thermal adaptation of species is important to both evolutionary biology and climate-change biology because it frequently leads to latitudinal gradients of various phenotypes among populations. Spotted sea bass (Lateolabrax maculatus) has a broad latitudinal distribution range along the marginal seas of the Northwest Pacific, providing an excellent teleost model for climate adaptation studies. We generated over 8.57 million SNP loci using whole genome re-sequencing from 100 samples collected at 14 geographic loci. We built the phylogeographic structure and demographic history of L. maculatus and determined sea surface temperature as the key environmental factor and major driving force for genetic divergence and local adaptation. We also identified distinct selective signatures and functional genes underlying adaptive mechanisms and ecological tradeoffs in the southernmost and northernmost populations inhabiting distinct climatic and latitudinal zones. The results offer an opportunity to better understand the genetic basis of the phenotypic variation in eurythermal fishes inhabiting different climatic regions.


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