scholarly journals Hypoglycemic, Antiglycation, and Cytoprotective Properties of a Phenol-Rich Extract From Waste Peel of Punica granatum L. var. Dente di Cavallo DC2

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3103 ◽  
Author(s):  
Antonella Di Sotto ◽  
Marcello Locatelli ◽  
Alberto Macone ◽  
Chiara Toniolo ◽  
Stefania Cesa ◽  
...  
Keyword(s):  
Ethyl Acetate Extract ◽  
Punica Granatum ◽  
Oxygen Species ◽  
Advanced Glycation ◽  
Total Glutathione ◽  
Pomegranate Peel ◽  
Phenolic Profile ◽  
Spectrophotometric Methods ◽  
Glycation End Products ◽  
Punica Granatum L

Pomegranate peel is a natural source of phenolics, claimed to possess healing properties, among which are antioxidant and antidiabetic. In the present study, an ethyl acetate extract, obtained by Soxhlet from the peel of Dente di Cavallo DC2 pomegranate (PGE) and characterized to contain 4% w/w of ellagic acid, has been evaluated for its hypoglycemic, antiglycation, and antioxidative cytoprotective properties, in order to provide possible evidence for future nutraceutical applications. The α-amylase and α-glucosidase enzyme inhibition, interference with advanced glycation end-products (AGE) formation, and metal chelating abilities were studied. Moreover, the possible antioxidant cytoprotective properties of PGE under hyperglycemic conditions were assayed. Phenolic profile of the extract was characterized by integrated chromatographic and spectrophotometric methods. PGE resulted able to strongly inhibit the tested enzymes, especially α-glucosidase, and exerted chelating and antiglycation properties. Also, it counteracted the intracellular oxidative stress under hyperglycemic conditions, by reducing the levels of reactive oxygen species and total glutathione. Among the identified phenolics, rutin was the most abundant flavonoid (about 4 % w/w). Present results suggest PGE to be a possible remedy for hyperglycemia management and encourage further studies to exploit its promising properties.

scholarly journals Hypoglycemic, Antiglycation and Cytoprotective Properties of A Phenol Rich Extract from Waste Peel of Punica Granatum L. Var. Dente Di Cavallo DC2

2019 ◽  
Author(s):  
Antonella Di Sotto ◽  
Marcello Locatelli ◽  
Alberto Macone ◽  
Chiara Toniolo ◽  
Stefania Cesa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ellagic Acid ◽  
Punica Granatum ◽  
Pomegranate Peel ◽  
Experimental Conditions ◽  
Phenolic Profile ◽  
Spectrophotometric Methods ◽  
Cell Functions ◽  
Glycation End Products ◽  
Alpha Glucosidase

Pomegranate peel is a natural source of phenolics, claimed to possess healing properties, among which antioxidant and antidiabetic. In line with this evidence, the ethyl acetate PGE extract, obtained by Soxhlet from the peel of Dente di cavallo DC2 variety and characterized by a 4% amount of ellagic acid, has been studied for its hypoglycemic, antiglycation and antioxidative cytoprotective properties, in order to support a possible further nutraceutical interest. The α-amylase and α-glucosidase enzyme inhibition, interference with advanced glycation end-products (AGE) formation and metal chelating abilities were evaluated as hypoglycemic mechanisms. Also, considering that oxidative stress is associated with hyperglycemia complications, PGE antioxidant cytoprotective properties under hyperglycemic conditions were assayed. Phenolic profile was characterized by integrated chromatographic and spectrophotometric methods. Under our experimental conditions, PGE strongly inhibited the tested enzymes, especially α-glucosidase, and exerted chelating and antiglycation properties. Also, it reduced both ROS and GSH levels under hyperglycemic conditions, thus suggesting its ability to support cell functions by counteracting intracellular oxidative stress. Along with ellagic acid, rutin was the major identified flavonoid (about 4 %) of PGE. Present results suggest PGE to be a possible remedy for hyperglycemia management and encourage further studies to exploit its promising properties.

scholarly journals Polyphenolic Profile and Antimicrobial Potential of Peel Extracts Obtained from Organic Pomegranate (Punica granatum L.) Variety “Mollar De Elche”

2020 ◽  
Vol 23 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Marina Cano-Lamadrid ◽  
Manuel Viuda-Martos ◽  
José Miguel García-Garví ◽  
Jesús Clemente-Villalba ◽  
Ángel A. Carbonell-Barrachina ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
High Performance ◽  
Ethyl Acetate Extract ◽  
Antibacterial Properties ◽  
Punica Granatum ◽  
Listeria Innocua ◽  
Pomegranate Peel ◽  
Main Component ◽  
Polyphenolic Profile ◽  
Punica Granatum L

AbstractThe aim of this work was to determine the polyphenolic profile and the antibacterial properties of extracts from organic pomegranate peel, to evaluate if it could be used as a potential antimicrobial ingredient to elaborated organic food. The antibacterial properties of different organic pomegranate peel extracts (ethyl acetate, acetone and methanol, with an increasing polarity) were tested against: Listeria innocua, Achromobacter denitrificans and Algaligenes faecalis. All extracts showed antimicrobial activity against all bacteria tested except ethyl acetate extract against A. denitrificans. The polyphenolic profile was determined by High Performance Liquid Chromatography (HPLC). Five compounds were identified. Punicagalin was the main component found in acetone and methanol extracts (7,939 µg.g−1 and 5,178 µg.g−1 of lyophilized sample, respectively). Ellagic acid was the main component found in ethyl acetate extract (171 µg.g−1 of lyophilized sample).

Optimization of subcritical water extraction of phenolic antioxidants from pomegranate (Punica granatum L.) peel by response surface methodology

2017 ◽  
Vol 9 (32) ◽  
pp. 4647-4656 ◽  
Author(s):  
Linlin Yan ◽  
Yungang Cao ◽  
Guangyao Zheng
Keyword(s):  
Response Surface Methodology ◽  
Subcritical Water ◽  
Punica Granatum ◽  
Water Extraction ◽  
Phenolic Antioxidants ◽  
Subcritical Water Extraction ◽  
Extraction Technology ◽  
Pomegranate Peel ◽  
Efficient Extraction ◽  
Punica Granatum L

Subcritical water extraction (SWE), a ‘green’ and efficient extraction technology, was applied to extract phenolic antioxidants from pomegranate peel in this study.

scholarly journals Advanced Glycation End Products and Oxidative Stress in a Hyperglycaemic Environment

2021 ◽  
Author(s):  
Akio Nakamura ◽  
Ritsuko Kawahrada
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Advanced Glycation End Products ◽  
Reactive Oxygen ◽  
Protein Glycation ◽  
Oxygen Species ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products ◽  
And Oxidative Stress

Protein glycation is the random, nonenzymatic reaction of sugar and protein induced by diabetes and ageing; this process is quite different from glycosylation mediated by the enzymatic reactions catalysed by glycosyltransferases. Schiff bases form advanced glycation end products (AGEs) via intermediates, such as Amadori compounds. Although these AGEs form various molecular species, only a few of their structures have been determined. AGEs bind to different AGE receptors on the cell membrane and transmit signals to the cell. Signal transduction via the receptor of AGEs produces reactive oxygen species in cells, and oxidative stress is responsible for the onset of diabetic complications. This chapter introduces the molecular mechanisms of disease onset due to oxidative stress, including reactive oxygen species, caused by AGEs generated by protein glycation in a hyperglycaemic environment.

Inhibition of advanced glycation end products by Punica granatum Linn. leaves and its antioxidant activity

2018 ◽  
Vol 18 (2) ◽  
pp. 97-105
Author(s):  
Snehal Nitin Mestry ◽  
Nitin Bhanudas Gawali ◽  
Malvika Sunil Gursahani ◽  
Sarayu Arvind Pai ◽  
Jayesh Bachu Dhodi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Advanced Glycation End Products ◽  
Punica Granatum ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products
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