scholarly journals Analysis of Flavonoid Metabolites in Citrus Peels (Citrus reticulata “Dahongpao”) Using UPLC-ESI-MS/MS

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2680 ◽  
Author(s):  
Fu Wang ◽  
Lin Chen ◽  
Hongping Chen ◽  
Shiwei Chen ◽  
Youping Liu
Keyword(s):  
Biological Activities ◽  
Antioxidant Properties ◽  
Principal Component ◽  
The Other ◽  
Citrus Reticulata ◽  
Flavonoid Content ◽  
Esi Ms ◽  
Medicinal Value ◽  
Citrus Peels ◽  
Citrus Varieties

Flavonoids are a kind of essential substance for the human body because of their antioxidant properties and extremely high medicinal value. Citrus reticulata “Dahongpao” (DHP) is a special citrus variety that is rich in flavonoids, however little is known about its systematic flavonoids profile. In the present study, the presence of flavonoids in five important citrus varieties, including DHP, Citrus grandis Tomentosa (HZY), Citrus ichangensis Swingle (YCC), Citrus sinensis (L.) Osbeck (TC), and Citrus reticulata ‘Buzhihuo’ (BZH), was determined using a UPLC-ESI-MS/MS-based, widely targeted metabolome. Results showed that a total of 254 flavonoid metabolites (including 147 flavone, 39 flavonol, 21 flavanone, 24 anthocyanins, 8 isoflavone, and 15 polyphenol) were identified. The total flavonoid content of peels from DHP was the highest. DHP could be clearly separated from other samples through clustering analysis and principal component analysis (PCA). Further, 169 different flavonoid metabolites were observed between DHP peels and the other four citrus peels, and 26 down-regulated differential metabolites displayed important biological activities in DHP. At the same time, a unique flavonoid component, tricin 4′-O-syringyl alcohol, was only found in DHP, which could be used as a marker to distinguish between other varieties. This work might facilitate a better understanding of flavonoid metabolites between DHP peels and the other four citrus peels and provide a reference for its sufficient utilization in the future.

scholarly journals Evaluation of The Physicochemical, Sensorial and Antioxidant Properties of Functional Ale Beer Brewed with Rice and Fruit by-Products

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Wichien Sriwichai ◽  
Pakkawat Detchewa ◽  
Patcharee Prasajak
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Principal Component ◽  
Banana Peel ◽  
Total Phenolic ◽  
Malt Extract ◽  
Flavonoid Content ◽  
Coffee Pulp ◽  
By Products ◽  
Rice Powder

Functional beer brewed with a high bioactive compound ingredients is beneficial to prevent many diseases. Rice and fruit by-products are rich in bioactive compounds and have a potential for functional beer production. The aim of the present work was to assess the physicochemical, nutritional and sensorial properties of the beer brewed with rice and fruit by-products. The five formulas of beer investigated were the dried malt extract, Phitsanulok paddy rice powder, riceberry powder, banana peel and coffee pulp. The ratio of the dried malt extract and the other alternative ingredients was 80:20. The contents in reducing sugar, total phenolic compounds, flavonoid, chlorogenic acid, caffeine and dietary fiber were evaluated. The correlations between the physicochemical, sensorial, antioxidant properties and acceptability of beers were assessed by the Principal Component Analysis. The results showed that beers were differed in color density and have a low alcohol content. Among beer formula, beers brewed with coffee pulp was the richest in total phenolic and beers brewed with banana peel was the richest in flavonoid content (278.82 mg gallic acid equivalent/ L beer and 69.18 mg catechin equivalent/ L respectively). The antioxidant capacity was the highest in banana peel beer formula (76.64%). The Principal Component Analysis revealed that beers brewed with the two varieties of rice powder formula were in the same group of beer control (dried malt extract formula). These beers were characterized by a high note of appreciation from consumer. Whereas, a high correlation between the antioxidant capacity and total polyphenolic, flavonoid content was observed in beer fruit by-products formulas. The incorporation of two varieties of rice powder or fruit by-products was feasible in brewing process to produce a functional ale beer appreciated by customer and having a noticeable antioxidant capacity.

scholarly journals Analysis of Flavonoid Metabolites in Buckwheat Leaves Using UPLC-ESI-MS/MS

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1310 ◽  
Author(s):  
Jing Li ◽  
Pu Yang ◽  
Qinghua Yang ◽  
Xiangwei Gong ◽  
Hongchi Ma ◽  
...  
Keyword(s):  
Clustering Analysis ◽  
Principal Component ◽  
Ultra Performance Liquid Chromatography ◽  
Tartary Buckwheat ◽  
Biological Functions ◽  
Common Buckwheat ◽  
Flavonoid Content ◽  
Esi Ms ◽  
Orthogonal Signal Correction ◽  
Signal Correction

Flavonoids from plants are particularly important in our diet. Buckwheat is a special crop that is rich in flavonoids. In this study, four important buckwheat varieties, including one tartary buckwheat and three common buckwheat varieties, were selected as experimental materials. The total flavonoid content of leaves from red-flowered common buckwheat was the highest, followed by tartary buckwheat leaves. A total of 182 flavonoid metabolites (including 53 flavone, 37 flavonol, 32 flavone C-glycosides, 24 flavanone, 18 anthocyanins, 7 isoflavone, 6 flavonolignan, and 5 proanthocyanidins) were identified based on Ultra Performance Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry (UPLC-ESI-MS/MS) system. Through clustering analysis, principal component analysis (PCA), and orthogonal signal correction and partial least squares-discriminant analysis (OPLS-DA), different samples were clearly separated. Considerable differences were observed in the flavonoid metabolites between tartary buckwheat leaves and common buckwheat leaves, and both displayed unique metabolites with important biological functions. This study provides new insights into the differences of flavonoid metabolites between tartary buckwheat and common buckwheat leaves and provides theoretical basis for the sufficient utilization of buckwheat.

scholarly journals Metabolomic Profiling and Biological Activities of Pleurotus columbinus Quél. Cultivated on Different Agri-Food Byproducts

Antibiotics ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1245
Author(s):  
Paola Angelini ◽  
Roberto Maria Pellegrino ◽  
Bruno Tirillini ◽  
Giancarlo Angeles Flores ◽  
Husam B. R. Alabed ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biological Activities ◽  
Biological Effects ◽  
Antioxidant Properties ◽  
Principal Component ◽  
Antimicrobial Activities ◽  
Biological Properties ◽  
Ms Analysis ◽  
Quantitative Hplc ◽  
Mushroom Extracts

The genus Pleurotus (Fr.) P. Kumm (Pleurotaceae, Basidiomycota) comprises a cosmopolitan group of mushrooms highly appreciated for their nutritional value and health-promoting benefits. Despite there being many studies about the phytochemical composition of Pleurotus spp., there are very few reports dealing with the phytochemistry, antioxidant and antimicrobial activities of P. columbinus Quél. In this study, a mass spectrometry ultra-performance liquid chromatography mass spectrometry (UHPLC)-QTOF method, coupled with principal component analysis (PCA), was applied to the P. columbinus metabolome in order to investigate the influence of different agri-food residues as growth substrates for P. columbinus cultivation, on the bioactive chemical profile of fruiting bodies and evaluated their potential as antioxidants and antimicrobials. Additionally, a quantitative HPLC-DAD-MS analysis was conducted on phenolic and flavonoid compounds, that could explain, albeit partially, the observed biological effects of P. columbinus extracts. The qualitative metabolic profile identified 97 metabolites, whereas the quantitative HPLC-DAD-MS analysis confirmed the presence of phenolic and flavonoids, in the mushroom extracts, which also showed intrinsic scavenging/reducing and antimicrobial effects. The antibacterial effects were particularly evident against Escherichia coli, whereas Tricophyton and Aspergillus were the dermatophytes more sensitive to the mushroom extracts. The present study supports more in-depth investigations, aimed at evaluating the influence of growth substrate on P. columbinus antimicrobial and antioxidant properties. The extracts from P. columbinus revealed valuable sources of primary and secondary metabolites, thus suggesting potential applications in the formulation of food supplements with biological properties, above all in terms of antioxidant and antimicrobial properties.

scholarly journals Natural Bioactive Cinnamoyltyramine Alkylamides and Co-Metabolites

Biomolecules ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1765
Author(s):  
Antonio Evidente ◽  
Marco Masi
Keyword(s):  
Natural Products ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Carbon Skeleton ◽  
The Other ◽  
Present Review ◽  
Natural Sources ◽  
Plant Kingdom ◽  
Structural Variability ◽  
Plant Families

Natural products are a vital source for agriculture, medicine, cosmetic and other fields. Among them alkylamides are a broad and expanding group found in at least 33 plant families. Frequently, they possess a simple carbon skeleton architecture but show broad structural variability and important properties such as immunomodulatory, antimicrobial, antiviral, larvicidal, insecticidal and antioxidant properties, amongst others. Despite to these several and promising biological activities, up to today, only two reviews have been published on natural alkylamides. One focuses on their potential pharmacology application and their distribution in the plant kingdom and the other one on the bioactive alkylamides specifically found in Annona spp. The present review is focused on the plant bioactive cinnamoyltyramine alkylamides, which are subject of several works reported in the literature. Furthermore, the co-metabolites isolated from the same natural sources and their biological activities are also reported.

scholarly journals Antioxidant Properties of Fresh and Frozen Peels of Citrus Species

2019 ◽  
Vol 7 (2) ◽  
pp. 331-339 ◽  
Author(s):  
Nur Fatin Inazlina Noor Azman ◽  
Azrina Azlan ◽  
Hock Eng Khoo ◽  
Muhammad Rizal Razman
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Citrus Species ◽  
Flavonoid Content ◽  
Citrus Peel ◽  
Citrus Peels

Citrus peel is a functional food. It is rich in antioxidants. This study aims to investigate the antioxidant properties of selected fresh and frozen peels of Citrus species. Frozen and fresh peels of lemon (Citrus limon), key lime (C. aurantifolia) and musk lime (C. microcarpa) were screened for their antioxidant properties such as total phenolic content and total flavonoid content. DPPH radical scavenging activity and ferric ion reducing antioxidant power (FRAP) assays were also determined. Among the three citrus peels, musk lime peel had the significantly highest total phenolic content and total flavonoid content. Frozen citrus peels showed significantly higher antioxidant content than the fresh peels. The frozen peels also showed promising antioxidant activity as indicated by their significantly higher FRAP value compared with fresh citrus peels. Moreover, frozen citrus peel possessed higher antioxidant activity as indicated by its lower EC50 values which ranged between 0.823 ± 0.1 and 3.16 ± 0.92 mg mL-1. A moderately high correlation was determined between FRAP value and total phenolic content (r=0.783), and between FRAP value and total flavonoid content. This study shows that frozen peels of citrus are functional foods and sources of potent antioxidants.

Determination and Evaluation of Flavonoids in Hawthorn in China

2020 ◽  
Vol 14 (5) ◽  
pp. 664-669
Author(s):  
Wenzhe Hou ◽  
Yuting Wang ◽  
Wei Wang ◽  
Meili Zhi ◽  
Xinlei Gou ◽  
...  
Keyword(s):  
High Performance ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
The Other ◽  
Agricultural Product ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Catechin Hydrate ◽  
Crataegus Pinnatifida ◽  
Hawthorn Fruit

Hawthorn (Crataegus pinnatifida Bunge) is a common agricultural product with applications in medicine and food in China. In particular, the flavonoids in hawthorn have important medicinal effects. To quantify the flavonoid contents in hawthorn fruit of different regions in China and scientifically guide the reasonable consumption of hawthorn, 12 flavonoids in hawthorn samples from Shandong (SD), Henan (HN), Shaanxi (SAX), Shanxi (SX), and Jiangsu (JS) were detected by means of high performance liquid chromatography (HPLC). The total flavonoid content in the Henan sample from hawthorn was the highest, at 177.94 μg · g−1, and the Jiangsu sample was the lowest at 124.67 μg · g−1. Epicatechin was the flavonoid with the highest concentration in samples from all regions, and the average content in Shandong samples reached 73.44 μg · g−1. Principal component analysis (PCA), hierarchical cluster analysis (HCA) and discriminant analysis (DA) were also performed to evaluate the effect of different growing regions in China on the contents of flavonoids in hawthorn. We found the Jiangsu samples had obvious regional characteristics, and the compositions of (±)-catechin hydrate, epicatechin and phlorizin dihydrate could be used to distinguish hawthorn grown in Jiangsu from the other four producing areas. The results will provide the reference basis for Chinese hawthorn consumers.

Effects of Saccharomyces Cerevisiae Strains on Chemical Profiles of Cabernet Sauvignon Wines: Based on the Combined Results of 1H NMR, HS-SPME/GC-MS and HPLC-DAD-ESI-MS/MS

2018 ◽  
Vol 18 (2) ◽  
pp. 115-131
Author(s):  
Liang Heng-Yu ◽  
Su Ning ◽  
Guo Kun ◽  
Wang Yuan ◽  
Yang De-Yu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Principal Component ◽  
Grape Juice ◽  
Pilot Scale ◽  
Ethyl Esters ◽  
Cabernet Sauvignon ◽  
Esi Ms ◽  
H Nmr ◽  
Chemical Profiles ◽  
Indigenous Yeasts

Five Saccharomyces cerevisiae strains (Chinese indigenous yeasts SC5, WC5, SC8, CC17 and commercial starter F15) were inoculated into Cabernet sauvignon grape must and fermented at pilot scale. For the first time, combination of 1H NMR, HS-SPME/GC-MS and HPLC-DAD-ESI-MS/MS metabonomic profiling techniques was performed to analyze the global chemical fingerprints of sampled wines at the end of alcoholic and malolactic fermentation respectively, then 13 non-volatile flavor compounds, 52 volatile organic aromas and 43 polyphenolic molecules were identified and determined correspondently. All principal component analysis (PCA) of two fermentation stages based on the analytical results of 1H NMR, HS-SPME/GC-MS and HPLC-DAD-ESI-MS/MS divided these strains into three clusters: (1) SC5 and SC8, (2) WC5 and F15 and (3) CC17. The wine fermented by indigenous yeast, CC17, showed a very unique chemical profile, such as low pH and high color intensity, reduced amino acids (including proline) and the lowest total higher alcohols levels, most of the fixed acids, glycerol, ethyl esters and anthocyanins concentrations. The statistical results indicate that CC17 strain possesses very special anabolism and catabolism abilities on such substances in grape juice and has potentiality to produce characteristic wines with high qualities.

HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species

2018 ◽  
Vol 69 (2) ◽  
pp. 305-309 ◽  
Author(s):  
Daniela Gitea ◽  
Simona Vicas ◽  
Manuel Alexandru Gitea ◽  
Sebastian Nemeth ◽  
Delia Mirela Tit ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Polyphenolic Compounds ◽  
The Other ◽  
The North ◽  
Western Area ◽  
North Western ◽  
Hypericum Species ◽  
Biological Actions

Our study compares the content in polyphenolic compounds and hypericin, in four species of Hypericum - H. perforatum L., H. maculatum Cr., H. hirsutum L., H. tetrapterum Fr. (syn. Hypericumacutum Mnch.) harvested from spontaneous flora in the north-western area of Transylvania, Romania. These species represent an important source of such compounds with different biological actions. After making the extracts, they were subjected to HPLC-SM analysis. The presence of rutoside in the largest amount (462.82 mg %) in the H. perforatum extract was observed, this containing most of the flavonoid heterosides. For the species H. maculatum, the presence in a much higher amount of the hyperoside (976.36 mg %) is characteristic compared to the other species. Quercetol is the best represented of the flavonoid aglycons, its concentration being the highest in H. hirsutum (659.66 mg %). The hypericin content ranges from 0.2171 g % in the H. tetrapterum extract, to 0.0314 g % in the methanol extract of H. maculatum.The highest antioxidant properties measured by FRAP method were recorded in the case of H. perforatum and H. maculatum.

Biological activities of two polypore macrofungi (Basidiomycota) and characterization of their compounds using HPLC–DAD and LC–ESI–MS/MS

2021 ◽  
Author(s):  
Samaneh Chaharmiri Dokhaharani ◽  
Masoomeh Ghobad-Nejhad ◽  
Hamid Moghimi ◽  
Abbas Farazmand ◽  
Hossein Rahmani
Keyword(s):  
Biological Activities ◽  
Esi Ms

scholarly journals Bioactive Alkaloids from Genus Aspergillus: Mechanistic Interpretation of Their Antimicrobial and Potential SARS-CoV-2 Inhibitory Activity Using Molecular Modelling

2021 ◽  
Vol 22 (4) ◽  
pp. 1866
Author(s):  
Fadia S. Youssef ◽  
Elham Alshammari ◽  
Mohamed L. Ashour
Keyword(s):  
Active Sites ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Marine Organisms ◽  
Topoisomerase Iv ◽  
Angiotensin Converting Enzyme 2 ◽  
Nucleotidyl Transferase ◽  
In Silico Studies ◽  
Best Fitting ◽  
Inhibitory Potential

Genus Aspergillus represents a widely spread genus of fungi that is highly popular for possessing potent medicinal potential comprising mainly antimicrobial, cytotoxic and antioxidant properties. They are highly attributed to its richness by alkaloids, terpenes, steroids and polyketons. This review aimed to comprehensively explore the diverse alkaloids isolated and identified from different species of genus Aspergillus that were found to be associated with different marine organisms regarding their chemistry and biology. Around 174 alkaloid metabolites were reported, 66 of which showed important biological activities with respect to the tested biological activities mainly comprising antiviral, antibacterial, antifungal, cytotoxic, antioxidant and antifouling activities. Besides, in silico studies on different microbial proteins comprising DNA-gyrase, topoisomerase IV, dihydrofolate reductase, transcriptional regulator TcaR (protein), and aminoglycoside nucleotidyl transferase were done for sixteen alkaloids that showed anti-infective potential for better mechanistic interpretation of their probable mode of action. The inhibitory potential of compounds vs. Angiotensin-Converting Enzyme 2 (ACE2) as an important therapeutic target combating COVID-19 infection and its complication was also examined using molecular docking. Fumigatoside E showed the best fitting within the active sites of all the examined proteins. Thus, Aspergillus species isolated from marine organisms could afford bioactive entities combating infectious diseases.

Sign in / Sign up

Export Citation Format

Share Document