scholarly journals Aldose Reductase, Protein Glycation Inhibitory and Antioxidant of Peruvian Medicinal Plants: The Case of Tanacetum parthenium L. and Its Constituents

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 2010 ◽  
Author(s):  
Seung Hwan Hwang ◽  
Hyun-Yong Kim ◽  
Yanymee N. Guillen Quispe ◽  
Zhiqiang Wang ◽  
Guanglei Zuo ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Aldose Reductase ◽  
Radical Scavenging ◽  
Protein Glycation ◽  
Dpph Radical ◽  
Sephadex Column ◽  
Impaired Wound Healing ◽  
Tanacetum Parthenium ◽  
Ic50 Values ◽  
Dpph Radical Scavenging

Diabetes complications, including peripheral neuropathy, cataracts, impaired wound healing, vascular damage, arterial wall stiffening and retinopathy diseases, are among the most predominant health problems facing the world’s population today. The 22 Peruvian plant extracts were screened for their potential inhibitory activity against rat lens aldose reductase (RLAR) and DPPH radical scavenging. Among them, we have found that Tanacetum parthenium L. (TP) has the RLAR, AGEs and DPPH radical scavenging activities. We used for screening of active components in TP against RLAR and DPPH for the first time by ultrafiltration (UF) and DPPH. Compounds in TP were isolated by Sephadex column chromatography and their structures were established by MS and NMR spectroscopic analyses. Among the isolated compounds, ferulic acid, apigenin, luteolin-7-O-glucoside, luteolin, chrysosplenol, and kaempferol showed potent inhibition with IC50 values of 1.11–3.20 and 6.44–16.23 μM for RLAR and DPPH radical scavenging. Furthermore, these compounds suppressed sorbitol accumulation in rat lenses and ferulic acid, luteolin-7-O-glucoside, and luteolin have AGEs inhibitory activities with IC50 values of 3.43–6.73 μM. In summary, our study provides interesting plants for further study with respect to the treatment and prevention of diabetic complication of Peruvian plant and can provide the scientific base of the traditional uses.

scholarly journals Phytochemical Screening and Evaluation of Antimicrobial and Antioxidant Activity of Mahonia napaulensis (Jamanemandro) Bark Extract

2021 ◽  
Vol 19 (2) ◽  
pp. 55-61
Author(s):  
Ranjan Paudel ◽  
Rabi Kiran Sharma ◽  
Shreeya Bhandari ◽  
Manan Koirala ◽  
Ganesh Bhandari ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Maximum Effect ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Ic50 Values ◽  
Dpph Radical Scavenging

Mahonia napaulensis also known as “Jamanemandro” in Nepali is a medium-sized stiff evergreen shrub widely distributed in South East Asia at an altitude of 1000-2000m, is traditionally used to treat dysentery and eye inflammation. This research focuses on screening of the phytochemicals, antimicrobial, and antioxidant properties of this plant. The methanolic extract was prepared using a Soxhlet apparatus. The antioxidant properties of extract were carried out by 50% inhibitory concentration (IC50) values from 2-2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. The phytochemical screening confirmed the presence of terpenoids, reducing sugars, tannins, alkaloids, glycosides including cardiac glycosides and steroids. The antimicrobial activity was studied using the disc diffusion method in five different human pathogens named Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi, and Shigella spps. The methanol extract was compared with standard chloramphenicol and showed that methanolic extract of is M.napaulensis exhibit maximum effect against S. aureus with higher growth inhibition zones (27.3 mm), followed by P. aeruginosa, Shigella spps., E. coli and S. typhi. These antimicrobial properties showed similar effect to that of positive control, chloramphenicol. The IC50 values from DPPH radical scavenging were 230.89 µg/mL and 182.73 µg/mL of methanol extract and ascorbic acid, respectively. Due to this antimicrobial and antioxidant properties of M. napaulensis it was widely applicable in biomedical field.

scholarly journals Evaluation of Aldose Reductase, Protein Glycation, and Antioxidant Inhibitory Activities of Bioactive Flavonoids in Matricaria recutita L. and Their Structure-Activity Relationship

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Seung Hwan Hwang ◽  
Zhiqiang Wang ◽  
Yanymee N. Guillen Quispe ◽  
Soon Sung Lim ◽  
Jae Myung Yu
Keyword(s):  
Aldose Reductase ◽  
Ex Vivo ◽  
Radical Scavenging ◽  
Protein Glycation ◽  
Matricaria Recutita ◽  
Structure Activity ◽  
Potent Inhibition ◽  
Glycation End Products ◽  
Inhibitory Activities ◽  
Dpph Radical Scavenging

The inhibitory activities of Matricaria recutita L. 70% methanol extract were evaluated by isolating and testing 10 of its compounds on rat lens aldose reductase (RLAR), advanced glycation end products (AGEs), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Among these compounds, apigenin-7-O-β-D-glucoside, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucuronide, luteolin-7-O-β-D-glucuronide, 3,5-O-di-caffeoylquinic acid, apigenin, and luteolin showed potent inhibition, and their IC50 values in RLAR were 4.25, 1.12, 1.16, 0.85, 0.72, 1.72, and 1.42 μM, respectively. Furthermore, these compounds suppressed sorbitol accumulation in rat lens under high-glucose conditions, demonstrating their potential to prevent sorbitol accumulation ex vivo. Notably, luteolin-7-O-β-D-glucuronide and luteolin showed antioxidative as well as AGE-inhibitory activities (IC50 values of these compounds in AGEs were 3.39 and 6.01 μM). These results suggest that the M. recutita extract and its constituents may be promising agents for use in the prevention or treatment of diabetic complications.

In Silico Design, Synthesis of Hybrid Combinations: Quercetin Based MAO Inhibitors with Antioxidant Potential

2019 ◽  
Vol 19 (2) ◽  
pp. 156-170
Author(s):  
Priyanka Dhiman ◽  
Neelam Malik ◽  
Anurag Khatkar
Keyword(s):  
Monoamine Oxidase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Scavenging Activity ◽  
Mao Inhibitors ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Ic50 Values ◽  
Dpph Radical Scavenging

<P>Background: Monoamine oxidase (MAO) is a critical target used for the cure of neuropsychological diseases. </P><P> Objective: A series of quercetin based derivatives was designed, synthesized, and evaluated as novel multifunctional agents against monoamine oxidase A and B with antioxidant potential. Methods: Hybrid derivatives based on quercetin were synthesized and screened for hMAO inhibition along with antioxidant activity. Molecular docking was performed to explicate the rationale of the different MAO (IC50) values and to explain the presence of inhibitory activity against specificity, respectively. Results: The results of in vitro hMAO inhibition showed that compound 8a, 6c, and 4 were found as potent hMAO-A inhibitors whereas compounds 6b, 6a, and 6d were observed as potent hMAO-B inhibitors. The DPPH radical scavenging activity showed that compounds 6b, 6a, and 4 exhibited a promising antioxidant potential with IC50 values 5.931±0.007, 6.421±0.037, and 8.516±0.098 respectively. Moreover, the compound 6b, 6a, and 4 exhibited remarkable H2O2 scavenging potential with IC50 values 05.80±0.004 µM, 06.20±0.009 µM, and 07.66±0.009 µM respectively. Conclusion: The results of docking studies were found in good correlation with experimental MAO inhibition studies. Moreover, the mechanistic insight into the docking poses was also explored by binding interactions of quercetin based derivatives inside the dynamic site of hMAO-A and hMAO-B. It was also noticed that the potent MAO inhibitors were also acting as better antioxidants as evaluated through DPPH radical scavenging activity and H2O2 radical scavenging assay.

scholarly journals Nutrients Content and Antioxidant Potential of Selected Traditional Vegetables Grown in Malawi

2021 ◽  
Vol 9 (2) ◽  
pp. 406
Author(s):  
Joseph Yohane Issa ◽  
Arnold Onyango ◽  
Anselimo Makokha ◽  
Judith Okoth
Keyword(s):  
Moringa Oleifera ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Leafy Vegetables ◽  
Corchorus Olitorius ◽  
Dpph Radical ◽  
Bidens Pilosa ◽  
Ipomea Batatas ◽  
Ic50 Values ◽  
Dpph Radical Scavenging

Vegetables provide nutrients, especially minerals and vitamins, and non-nutrient phytochemicals with health benefits. Iron, zinc and vitamin A deficiencies are common in most developing countries yet traditional vegetables that can provide these nutrients grow wildly in most areas. This study evaluated the nutrient contents, phytochemicals and the 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activities of five traditional leafy vegetables grown in Malawi, namely Amaranth hybridus (Amaranth ssp.), Moringa oleifera ( Drum stick), Bidens pilosa (Black jack), Corchorus olitorius (Jute mallow) and Ipomea batatas (Sweet potato) leaves. Results from the present study show that beta-carotene ranged from 829.68 µg/100g to 5,791.64 µg/100g and Moringa oleifera had the highest concentration. Vitamin C content ranged from 7.93 mg/100g to 35.81 mg/100g and Ipomea batatas leaves had the highest concentration. Zinc concentrations ranged from 4.42 mg/100g to 10.59 mg/100g. Iron content ranged from 37.22 mg/100g to 48.21 mg/100g. Total phenolics content (TPC) ranged from 0.98 g/100g to 3.89 g/100g with Bidens pilosa leaves having the highest TPC (3.89±0.23 g/100g). Moringa oleifera and Bidens pilosa leaves had significantly higher (P<0.05) total flavonoids content (TFC) compared to the other vegetables. Total carotenoids content (TCC) ranged from 1,708.68 µg/100g to 15,041.59 µg/100g. Tannins content ranged from 42.94 mg/100g to 77.16 mg/100g. DPPH radical scavenging activities of the vegetable leaves were in the order of Bidens pilosa > Corchorus olitorius > Moringa oleifera > Ipomea batatas > Amaranth hybridus. The 50% inhibitory concentration (IC50) values of these vegetables ranged from 12.5 µg/mL to 125 µg/mL. Lower IC50 values indicate higher radical scavenging activity. The results suggested that the traditional vegetables under the study can significantly contribute to improved human nutrition and have potential to reduce diseases related to oxidative stress.

scholarly journals 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of extracts from Aloiampelos striatula

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2062-2066
Author(s):  
K.T. Mokoroane ◽  
M.K. Pillai ◽  
S. Magama
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Positive Control ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Water Extracts ◽  
Ic50 Values ◽  
Commercial Applications ◽  
Dpph Radical Scavenging

Hexane, chloroform, acetone, methanolic and water extracts from leaves and stem-bark of Aloiampelos striatula were evaluated for their antioxidant activity by 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging assay. The hexane, chloroform, acetone, methanolic and water extracts from leaves of A. striatula showed scavenging activity ranging from 10.30±0.41 - 30.14±1.4, 30.49±0.85 - 45.95±0.22, 38.45±0.49 - 51.65±0.95, 34.83±1.53 - 64.98±0.45 and 54.37±1.24 - 66.74±0.89%, respectively, at various concentrations. The hexane, chloroform, acetone, methanolic and water extracts from stem -bark of A. striatula showed scavenging activity ranging from 12.53±3.07 - 29.81±2.43, 17.80±5.17 - 37.20±2.13, 6.15±0.24 - 62.79±3.82, 38.23±1.18 - 67.32±0.55 and 3.16±0.40 - 70.66±0.18%, respectively, at various concentrations. Additionally, the IC50 values of these extracts were also determined and were found to be in the range of <200 to >3000 µg/mL. The positive control, vitamin C, showed an IC50 value of <200 µg/mL. The therapeutic applications of A. striatula are often in traditional Basotho medicine in the Kingdom of Lesotho, which include treating skin problems, high blood pressure, sexually transmitted illnesses, common colds, blood cleansing, stomach ailments and dressing of wounds. Further studies on this plant are required to explore this plant for its commercial applications.

scholarly journals Antioxidant and hypoglycemic activities of various solvent fractions of methanol extract of Terminalia alata Heyne ex Roth trunk-bark

2021 ◽  
Vol 20 (1) ◽  
pp. e748
Author(s):  
Vinh Nguyen Quang ◽  
Pham Van Hung ◽  
Anh Dzung Nguyen
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Methanol Extract ◽  
Fasting Blood Glucose ◽  
Radical Scavenging ◽  
Diabetic Rats ◽  
Dpph Radical ◽  
Ic50 Values ◽  
Phenolics Content ◽  
Dpph Radical Scavenging

The antioxidant and hypoglycemic capacities of various solvent fractions from the trunk-bark methanol extract of Terminalia alata Heyne ex Roth (T. alata) were investigated. The DPPH radical scavenging assay was used to evaluate the antioxidant activity, and the methods for determination of digestive enzymes inhibitory activity and fasting blood glucose reduction capacity in diabetic rats were used to determine the hypoglycemic activity of the extract fractions. The results indicated that higher total phenolics content was measured with increasing polarity of extraction solvent and DPPH radical scavenging activity coincided with phenolics content. Ethyl acetate fraction (EAF), n-butanol fraction (BF), and water fraction (WF) were obtained. They possessed α-amylase inhibition with the IC50 values of 0.056 ± 0.001, 0.138 ± 0.005, and 0.022 ± 0.001 mg.mL-1, respectively, which were lower than acarbose (IC50 = 0.154 ± 0.02 mg.mL-1). In contrast, IC50 values of α-glucosidase inhibitory activities of these fractions were higher than those of acarbose. In addition, these fractions also lowered fasting blood glucose concentrations in streptozotocin induced diabetic rats at a dose of 200 mg.kg-1 bw (body weight) without inducing body weight loss, which was not observed when treating with acarbose. The EAF and WF of trunk-bark of T. alata are recommended as potent sources for further research on antioxidant and hypoglycemic activities.

scholarly journals Antioxidant Activity of Different Forms of Green Tea: Loose Leaf, Bagged and Matcha

2018 ◽  
Vol 6 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Sumaya Farooq ◽  
Amit Sehgal
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Significant Difference ◽  
Ic50 Values ◽  
Dpph Radical Scavenging

Green tea is commercially available in three forms: loose leaf, bagged and powdered. The objective of this study was to compare the radical scavenging capacity of different forms of green tea like loose leaf (3), bagged (2) and powdered matcha (2) of various brands. The green tea forms were prepared at 95-100°C for 5 min., to mimic conditions usually used for tea preparations at home. The comparison of combined IC50 values of different green tea forms (loose leaf, bagged and matcha) showed no significant difference in their radical scavenging activity except bagged tea that exhibited slightly more DPPH radical scavenging potential as compared to matcha. Individually, the Bud white loose leaf demonstrated highest antioxidant activity followed by Laplant bag, Lipton bag, Laplant loose, Gourmet matcha, Wow matcha and Lipton loose. These findings revealed that on the basis of form, it may not be possible to generalize which form of tea whether loose leaf, bagged or matcha, is more effective in scavenging free radicals.

In vitro Antioxidant potential of Sphaeranthus indicus and Abrus precatorius leaves extracts

2021 ◽  
pp. 3821-3825
Author(s):  
Ashutosh Pal Jain ◽  
Gajendra Pratap Choudhary
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Dpph Radical ◽  
Abrus Precatorius ◽  
Reducing Ability ◽  
Ic50 Values ◽  
Dpph Radical Scavenging

Background: The purpose of this study was to evaluate the antioxidant potential of Sphaeranthus indicus and Abrus precatorius leaves. Methods: Both plant leaves powder was exhaustively extracted by soxhlet apparatus with various solvents of increasing order of polarity, petroleum ether followed by chloroform, methanol and water. The total phenol, tannins and flavonoids content were determined spectrophotometrically. Quantitative estimation of phenolic and flavonoid compounds in the extracts was estimated by using the Folin–Ciocalteu reagent and aluminum chloride colorimetric method respectively. The antioxidant potential of all these extracts was evaluated by reducing ability assay and DPPH radical scavenging assay with vitamin C as a standard. The antioxidant activity increased with dose dependence of extracts (from 20 to 200μg/ml). Results: The order for reducing power of extracts was recorded as vitamin C > methanol > water ≈ chloroform > petroleum ether and the same order was found in the DPPH assay having 50% inhibitory concentration (IC50) values. The results revealed that the methanolic extract exhibited the highest percent inhibition of the reducing ability and DPPH radical scavenging assay as compared to the other extracts of both plant. Moreover, methanolic extract showed concentration dependent significant scavenging of DPPH, especially with S. indicus [IC50 values 112.23±0.39 μg/ml] and A. precatorius [IC50 values 101.44±0.41μg/ml], while that of the standard was 13.45±0.37μg/ml (vitamin C). Methanolic extract showed phenolics, flavonoids and tannins contents were significantly higher than all the extracts investigated in both plant. Conclusions: A correlation between the antioxidant activity and the total phenolic contents of the extracts indicated that phenolic compounds were the dominant contributors to the antioxidant activity of the plant. The results suggest that methanolic leaves extract of S. indicus and A. precatorius are source of natural antioxidants and free radical scavenging capability.

ANTIOXIDANT ACTIVITY AND ACUTE TOXICITY OF EXTRACT OF UVARIA GRANDIFLORA ROXB. EX HORNEM - ANNONACEAE&NBSP;

2018 ◽  
Vol 8 (3) ◽  
pp. 95-99
Author(s):  
Thi Hoai Nguyen
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Central Vietnam ◽  
Ic50 Values ◽  
Ld50 Value ◽  
Dpph Radical Scavenging

Background: Uvaria grandiflora, a naturally occurring medicinal plant in Central Vietnam, showed many significant biological activities. The objective of the study was to evaluate the antioxidant activity through the ability of Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and the acute toxicity of extract of U. grandiflora. Materials: The aerial part of U. grandiflora. Method: Evaluation the ability of DPPH radical scavenging by the method of Kedare (2011). Evaluation of acute toxicity by the method of Abraham and Turner (1978). Results: The crude methanol extract and ethyl acetat fraction displayed the IC50 values 88.49 µg/ml and 65.57 µg/ml, respectively. On acute toxicity, the LD50 value could not be identified at the maximum dose given to mice (10000 mg/kg mice). Conclusion: Extract of methanol and ethyl acetat fraction of Uvaria grandiflora showed moderate antioxidant activities. The LD50 value could not be identified. Key words: antioxidant, acute toxictity, DPPH, free radical, Uvaria grandiflora

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