scholarly journals Xylopia aethiopica Seeds from Two Countries in West Africa Exhibit Differences in Their Proteomes, Mineral Content and Bioactive Phytochemical Composition

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1979 ◽  
Author(s):  
Xiaojian Yin ◽  
María A.S.C. Chávez León ◽  
Richard Osae ◽  
Loveth O. Linus ◽  
Lian-Wen Qi ◽  
...  

Aside from its multiple medicinal uses, the fruit of Xylopia aethiopica is widely used in Africa as food. Herein, we characterize the protein profiles, mineral content and bioactive phytochemical composition of the seeds of this plant sourced in Ghana and Nigeria. Using label-free proteomics, a total of 677 proteins were identified, with 260 found in the Ghana-sourced samples while 608 proteins were detected in the samples from Nigeria. However, 114 proteins were common between the samples from the two countries, among which 48 were significantly changed. Bioinformatics and functional analyses revealed that the differential levels of the proteins were mainly linked to pathways involved amino acids metabolism and biosynthesis. The significantly changed proteins related mainly to catalytic activity and carbon metabolism. The samples from Nigeria also exhibited superior qualities in terms of their antioxidant effects, and total phenolic and flavonoid content. Finally, only the content of Na varied to a statistically significant level. This study lends support to its culinary use and hints towards the impact of location of cultivation on the quality of the seeds. There is however need for further mechanistic investigations to unravel the underlying reasons for the observed differences.

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1840
Author(s):  
Aleksandra Matys ◽  
Artur Wiktor ◽  
Magdalena Dadan ◽  
Dorota Witrowa-Rajchert

The conditions of convective drying induce a negative effect on the quality of the dried products, and thus, innovative solutions, such as the utilization of ultrasound and dehumidified air are gaining importance. Therefore, the aim of this study was to evaluate the impact of ultrasound pretreatment and variable air temperature on the kinetics of convective drying with dehumidified air and the quality of dried apples. Apples were dried with dehumidified and non-dehumidified air at 55, 70 and 85 °C. Preliminary ultrasound treatment was conducted by immersion for 60 min. The effect of both ultrasound and dehumidified air utilization was more prominent in the terms of drying time reduction, when lower drying temperatures were used. Drying of apples with dehumidified air, preceded by ultrasound pretreatment, resulted in a greater rehydration capacity of the products, and limited the browning process. Dehumidified air increased the lightness of the dried products, while sonication darkened them. The use of ultrasound before drying coupled with a drying with dehumidified medium at a temperature of 70 °C reduced the exposure of the product to a high temperature and oxygen. Products treated before dehumidified air drying with ultrasounds were characterized by high total phenolic content and the greatest antioxidant activity. This was because such technological treatment reduced exposure of the product to a high temperature and oxygen.


2020 ◽  
Vol 50 (9) ◽  
Author(s):  
Paula Juliana Grotto Débia ◽  
Beatriz Cervejeira Bolanho Barros ◽  
Heriksen Higashi Puerari ◽  
Claudia Regina Dias-Arieira

ABSTRACT: Meloidogyne javanica is a plant-parasitic nematode that infects a wide range of vegetables. Its negative effects on crop yield and value are well documented. However, few studies have investigated the impact of the parasite on the nutritional value of vegetables. This study aimed to assess the effect of M. javanica parasitism on the vegetative characteristics, nematological parameters, chemistry composition and antioxidant activity of carrots. Seedlings were inoculated with 0 (control), 1000, 2500, or 5000 eggs and eventual second-stage juveniles (J2) of M. javanica. At 60 days after inoculation, plants were harvested and evaluated. Plants inoculated with 2500 eggs and J2 of M. javanica had higher root and tuber fresh weight than the control. Gall number increased with increasing inoculum density. The number of nematodes in the roots increased until 3000 specimens, decreasing thereafter. Proximate analysis revealed that plants inoculated with 1000 eggs and J2 of M. javanica or more had higher protein content in roots. In contrast, inoculation with 1775 nematodes or more resulted in a decrease in carotenoid content. There was no effect of inoculation on total phenolic content or antioxidant activity. Although, M. javanica infection did not have a marked impact on the nutritional quality of carrots, gall formation resulted in deformed roots of low commercial value.


Toxins ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 369 ◽  
Author(s):  
Zhao Jin ◽  
James Gillespie ◽  
John Barr ◽  
Jochum Wiersma ◽  
Mark Sorrells ◽  
...  

This project was initiated with the goal of investigating the malt quality of winter rye cultivars and hybrids grown in the United States in 2014 and 2015, but high levels of deoxynivalenol (DON) were subsequently found in many of the malt samples. DON levels in 75% of the investigated rye samples (n = 117) were actually below 1.0 mg/kg, as quantified by a gas chromatography combined with electron capture detector (GC-ECD). However, 83% of the samples had DON in excess of 1.0 mg/kg following malting, and the average DON level in malted rye was 10.6 mg/kg. In addition, relatively high levels of 3-acetate DON (3-ADON), 15-acetate DON (15-ADON), nivalenol (NIV), and DON-3-glucoside (D3G) were observed in some rye malts. Our results show that rye grain DON is likely a poor predicator of type B trichothecenes in malt in practice, because high levels of malt DON, 15-ADONm and D3G were produced, even when the rye samples with DON levels below 0.50 mg/kg were processed. Fusarium Tri5 DNA content in rye was highly associated with malt DON levels (r = 0.83) in a small subset of samples (n = 55). The impact of Fusarium infection on malt quality was demonstrated by the significant correlations between malt DON levels and wort viscosity, β-glucan content, wort color, wort p-coumaric acid content, and total phenolic content. Additional correlations of rye Fusarium Tri5 DNA contents with malt diastatic power (DP), wort free amino nitrogen (FAN) content, and arabinoxylan content were observed.


Author(s):  
Isaac John Umaru ◽  
Benedict Samling ◽  
Hauwa A. Umaru

Objective: This study was carried out with an objective to investigate the impact of biochar on phytochemical composition in plant especially Etlingera elatior cultivated on different dosage of biochar. Methods: Etlingera elatior was cultivated on the pot with 20 cm diameter and 35 cm height. 3 replicates for pots of Etlingera elatior was cultivated and label as 0%, 5% and 20%. The ordinary farm soil without biochar (0%) as control, fertilized soil with biochar; 5% and 20%. In a net house and watered twice daily. The gas chromatography mass spectrometry analysis was performed by using a non-polar BPX-5 capillary column with an initial temperature of 50°C hold for five minutes and then increased to 300°C at a rate of 5.0°C per minutes and hold 10 minutes. The biochar samples were analysed using an ATR-FTIR equipped with diamond crystal, controlled by OMNIC software (Thermo Nicolet Analytical Instruments, Madison, WI). A flat tip powder press was used to achieve even distribution and contact. Results: The result showed significantly increased in the phytochemical composition with increase in the biochar concentration. At 0% phytol (13%), Hexadecanoic acid (9.76%), Neophytadiene (6.51%), coumarin (5.65%), precocene (5.27%) and caryophyllene (4.59%). At 5% are Dihydrocucurbitacin (13.69%), Niacinamide (11.02%), α-Limonene (10.01%), Phyrahen (9.23%), Phytol (7.24%) and Neophytadiene (5.75%) and at 20% Linoleic acid (39.98%), 2-pinen-4-ol (12.32%), Hexatriacontyl pentafluoropropionate (6.89%), Benzofuran (5.12%), Acethophenon (4.41%) and furfural (4.03%). Conclusion: Application of biochar on soil can increase nutrient availability and enhance the development of phytochemical composition in plants. Without biochar, the chemical composition Etlingera elatior extract was slighlyt low. At 5% and 20% biochar, some compounds are increasing and new were obtained compared to 0% biochar. Most of the compounds known to be secondary metabolite which are rich in medicinal values. Thus, biochar could be used to increase the quantity and quality of phytochemicals in plant especially medicinal plants.


Author(s):  
CHAIYAVAT CHAIYASUT ◽  
PERIYANAINA KESIKA ◽  
Sasithorn Sirilun ◽  
Netnapa Makhamrueang ◽  
Sartjin Peerajan ◽  
...  

Objective: The present study evaluated the impact of drying and storage conditions on the quality of the avocado oil. Methods: The fresh avocado was obtained from local farmers of Chiang Mai province, Thailand. The avocado pulp was collected and blended. The blended samples were dried at different temperatures (60, 80, and 100°C) and the oil was extracted by manual or mechanical pressing. The avocado oil samples were stored in clear and amber glass bottles at various temperatures (4, 30, and 40°C) for 3 months. The organoleptic, Folin–Ciocalteu colorimetric, 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid assay, and titration methods were used to measure the physical changes (color, odor, and precipitation), total phenolic content (TPC), antioxidant activity, and acid value (AV) and peroxide values (PVs) of the samples, respectively. Results: The manual squeezing and mechanical pressing methods yielded about 1.17 and 24.91% of avocado oil from fresh, undried avocado flesh, and the pH of both samples was 5. The AV of manually squeezed and mechanically pressed oil samples was 2.91 and 2.88 mg KOH/g of oil, respectively. The PV of manually squeezed and mechanically pressed oil samples was 7.96 and 8.03 milliequivalent of oxygen per Kg of oil, respectively. The quality of the avocado oil dried at 60°C was high compared to other samples regarding TPC and antioxidant capacity. The storage of oil samples for 3 months at various temperatures and in different containers has not affected the quality significantly, except at 40°C. Conclusion: The study proposed that the drying process at more than 80°C interferes with the quality of the avocado oil, and the storage conditions have a greater impact on the stability of the avocado oil. The storage at a low temperature in the absence of light may extend the shelf life of the avocado oil.


2019 ◽  
Vol 16 (1-2) ◽  
Author(s):  
Joanna Zubernik ◽  
Magdalena Dadan ◽  
Joanna Cichowska ◽  
Dorota Witrowa-Rajchert

AbstractThe aim of this study was to investigate the effects of pre-treatment in ethanol solution carried out in variable time with and without ultrasound (US) assistance on convective drying kinetics, total phenolic content (TPC) and hygroscopicity of an apple. The drying time after immersion in ethanol solution was shorter up to 13.4 (without US) and 18.3 % (with US) in comparison to intact slices. The most appropriate model that described kinetics of drying was the one proposed by Sledz et al. Drying of the untreated apple resulted in a decrease of the TPC by 18 %, compared with raw apples and the pre-treatment led to further losses. The tissue after ethanol pre-treatment was less hygroscopic, which proves the possibility to maintain a characteristic texture of the dried apple with prolonged storage stability. The best quality of dried apples was obtained after 1 min of ethanolic pre-treatment with US.


2020 ◽  
Vol 29 (4) ◽  
pp. 2097-2108
Author(s):  
Robyn L. Croft ◽  
Courtney T. Byrd

Purpose The purpose of this study was to identify levels of self-compassion in adults who do and do not stutter and to determine whether self-compassion predicts the impact of stuttering on quality of life in adults who stutter. Method Participants included 140 adults who do and do not stutter matched for age and gender. All participants completed the Self-Compassion Scale. Adults who stutter also completed the Overall Assessment of the Speaker's Experience of Stuttering. Data were analyzed for self-compassion differences between and within adults who do and do not stutter and to predict self-compassion on quality of life in adults who stutter. Results Adults who do and do not stutter exhibited no significant differences in total self-compassion, regardless of participant gender. A simple linear regression of the total self-compassion score and total Overall Assessment of the Speaker's Experience of Stuttering score showed a significant, negative linear relationship of self-compassion predicting the impact of stuttering on quality of life. Conclusions Data suggest that higher levels of self-kindness, mindfulness, and social connectedness (i.e., self-compassion) are related to reduced negative reactions to stuttering, an increased participation in daily communication situations, and an improved overall quality of life. Future research should replicate current findings and identify moderators of the self-compassion–quality of life relationship.


2016 ◽  
Vol 1 (13) ◽  
pp. 162-168
Author(s):  
Pippa Hales ◽  
Corinne Mossey-Gaston

Lung cancer is one of the most commonly diagnosed cancers across Northern America and Europe. Treatment options offered are dependent on the type of cancer, the location of the tumor, the staging, and the overall health of the person. When surgery for lung cancer is offered, difficulty swallowing is a potential complication that can have several influencing factors. Surgical interaction with the recurrent laryngeal nerve (RLN) can lead to unilateral vocal cord palsy, altering swallow function and safety. Understanding whether the RLN has been preserved, damaged, or sacrificed is integral to understanding the effect on the swallow and the subsequent treatment options available. There is also the risk of post-surgical reduction of physiological reserve, which can reduce the strength and function of the swallow in addition to any surgery specific complications. As lung cancer has a limited prognosis, the clinician must also factor in the palliative phase, as this can further increase the burden of an already compromised swallow. By understanding the surgery and the implications this may have for the swallow, there is the potential to reduce the impact of post-surgical complications and so improve quality of life (QOL) for people with lung cancer.


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