scholarly journals UHPLC-UV/Vis Quantitative Analysis of Hydroxylated and O-prenylated Coumarins in Pomegranate Seed Extracts

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1963 ◽  
Author(s):  
Serena Fiorito ◽  
Federica Ianni ◽  
Francesca Preziuso ◽  
Francesco Epifano ◽  
Luca Scotti ◽  
...  
Keyword(s):  
Punica Granatum ◽  
Biologically Active ◽  
Experimental Conditions ◽  
Analytical Methodology ◽  
Extraction Solvent ◽  
Versatile Technique ◽  
Efficient Extraction ◽  
Pomegranate Seed ◽  
Set Up ◽  
Seed Extracts

A simple and rapid analytical UHPLC methodology with spectrophotometric (UV/Vis) detection, coupled with different extraction procedures, has been perfected to investigate the presence of biologically active O-prenylated umbelliferone derivatives, such as auraptene and umbelliprenin, in pomegranate (Punica granatum L.) seed extracts. Absolute ethanol was the most efficient extraction solvent in terms of yields, after a short ultrasound-assisted. The highest concentration values recorded under these experimental conditions were 1.99 μg/g of dry extract and 6.53 μg/g for auraptene and umbelliprenin, respectively. The parent metabolite umbelliferone was also detected (0.67 μg/g). The extraction and UHPLC analytical methodology set up in the present study proved to be an efficient, powerful, and versatile technique for the simultaneous qualitative analysis and quantification of oxyprenylated coumarins in pomegranate seed extracts. The characterization of such secondary metabolites in the mentioned phytopreparation represents, to the best of our knowledge, the first example in the literature.

scholarly journals Application of Bar Adsorptive Microextraction for the Determination of Levels of Tricyclic Antidepressants in Urine Samples

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3101
Author(s):  
Mariana N. Oliveira ◽  
Oriana C. Gonçalves ◽  
Samir M. Ahmad ◽  
Jaderson K. Schneider ◽  
Laiza C. Krause ◽  
...  
Keyword(s):  
Tricyclic Antidepressants ◽  
Matrix Effects ◽  
Activated Carbons ◽  
Process Efficiency ◽  
Gas Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Experimental Conditions ◽  
Analytical Methodology ◽  
Low Efficiency

This work entailed the development, optimization, validation, and application of a novel analytical approach, using the bar adsorptive microextraction technique (BAμE), for the determination of the six most common tricyclic antidepressants (TCAs; amitriptyline, mianserin, trimipramine, imipramine, mirtazapine and dosulepin) in urine matrices. To achieve this goal, we employed, for the first time, new generation microextraction devices coated with convenient sorbent phases, polymers and novel activated carbons prepared from biomaterial waste, in combination with large-volume-injection gas chromatography-mass spectrometry operating in selected-ion monitoring mode (LVI-GC-MS(SIM)). Preliminary assays on sorbent coatings, showed that the polymeric phases present a much more effective performance, as the tested biosorbents exhibited low efficiency for application in microextraction techniques. By using BAμE coated with C18 polymer, under optimized experimental conditions, the detection limits achieved for the six TCAs ranged from 0.2 to 1.6 μg L−1 and, weighted linear regressions resulted in remarkable linearity (r2 > 0.9960) between 10.0 and 1000.0 μg L−1. The developed analytical methodology (BAμE(C18)/LVI-GC-MS(SIM)) provided suitable matrix effects (90.2–112.9%, RSD ≤ 13.9%), high recovery yields (92.3–111.5%, RSD ≤ 12.3%) and a remarkable overall process efficiency (ranging from 84.9% to 124.3%, RSD ≤ 13.9%). The developed and validated methodology was successfully applied for screening the six TCAs in real urine matrices. The proposed analytical methodology proved to be an eco-user-friendly approach to monitor trace levels of TCAs in complex urine matrices and an outstanding analytical alternative in comparison with other microextraction-based techniques.

Optimization of subcritical water extraction of phenolic antioxidants from pomegranate (Punica granatum L.) peel by response surface methodology

2017 ◽  
Vol 9 (32) ◽  
pp. 4647-4656 ◽  
Author(s):  
Linlin Yan ◽  
Yungang Cao ◽  
Guangyao Zheng
Keyword(s):  
Response Surface Methodology ◽  
Subcritical Water ◽  
Punica Granatum ◽  
Water Extraction ◽  
Phenolic Antioxidants ◽  
Subcritical Water Extraction ◽  
Extraction Technology ◽  
Pomegranate Peel ◽  
Efficient Extraction ◽  
Punica Granatum L

Subcritical water extraction (SWE), a ‘green’ and efficient extraction technology, was applied to extract phenolic antioxidants from pomegranate peel in this study.

Studies on the use of cultured cells in a bioassay for parathyroid hormone

1994 ◽  
Vol 143 (2) ◽  
pp. 261-268
Author(s):  
A E Armston ◽  
P J Wood
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Cell Line ◽  
Cultured Cells ◽  
Biologically Active ◽  
Kidney Cell Line ◽  
Opossum Kidney ◽  
Pre Treatment ◽  
Set Up ◽  
Insufficient Sensitivity

Abstract Measurement of parathyroid hormone (PTH) is important for diagnosing hyper- and hypoparathyroidism. The move to two-site immunometric assays that detect the whole molecule has improved the discrimination of these conditions but these assays may be too restrictive because some PTH fragments that are biologically active may not be detected. In addition, PTH-like peptide of malignancy, an important cause of malignancy-associated hypercalcaemia, is not detected by the two-site assays. Experiments were performed to set up a simple, robust and inexpensive bioassay for PTH, exploiting a kidney cell line and using cyclic AMP or an eluted stain assay as the end point. Of the 12 cell lines tested, an opossum kidney (WOK) cell line showed the most promise. Despite optimization of the procedure to include pre-treatment with dexamethasone, insulin and PTH, followed by incubation in the presence of 5′ -guanylimidodiphosphate, isobutyl-1-methylxanthine and forskolin, the WOK cells showed insufficient sensitivity for use in a cultured cell bioassay for PTH in human serum. In addition, the cells were less sensitive to PTH-like peptide precluding their use for an assay for this molecule. Journal of Endocrinology (1994) 143, 261–268

scholarly journals Synthesis of New Antibiotics Derivatives by the Photocatalytic Method: A Screening Research

Catalysts ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1102
Author(s):  
Wojciech Baran ◽  
Ewa Masternak ◽  
Dominika Sapińska ◽  
Andrzej Sobczak ◽  
Ewa Adamek
Keyword(s):  
Quantum Yield ◽  
Biologically Active ◽  
Quantum Yields ◽  
Photocatalytic Process ◽  
Experimental Conditions ◽  
New Antibiotics ◽  
Photocatalytic Reactions ◽  
Kinetics Of ◽  
Derivatives Of ◽  
Uva Radiation

The aim of our study was to assess the possibility of using the photocatalytic process conducted in the presence of TiO2 to obtain new stable derivatives of antibacterial drugs. The possibility of introducing hydroxyl, chlorine, or bromide groups into antibiotics molecules was investigated. The experiments were conducted in aqueous solutions in the presence of TiO2-P25 as a photocatalyst, Cl− and Br- ions, and antibiotics belonging to eight different chemical classes. All experiments were initiated by UVa radiation. The kinetics of photocatalytic reactions and their quantum yield were determined, and the stable products were identified. All of the antibiotics used in the experiments underwent a photocatalytic transformation, and the quantum yields were in the range from 0.63 to 22.3%. The presence of Br- or FeCl3 significantly increased the efficiency of the photocatalytic process performed in the presence of TiO2, although Br- ion also acted as an inhibitor. Potentially biologically active chlorine derivatives from Trimethoprim, Metronidazole, Chloramphenicol, and bromine derivatives from Trimethoprim, Amoxicillin were obtained under experimental conditions. The potentially inactive halogen derivatives of Sulfamethoxazole and hydroxyl derivatives described in the literature were also identified.

Antibiotic treatment of the breadcrumb sponge Halichondria panicea and subsequent recolonization v1

2021 ◽  
Author(s):  
Lara Schmittmann ◽  
Ute U Hentschel
Keyword(s):  
Antibiotic Treatment ◽  
Recovery Phase ◽  
Phase 3 ◽  
Experimental Conditions ◽  
Host Environment ◽  
Halichondria Panicea ◽  
Set Up ◽  
Bacterial Recolonization ◽  
Sponge Halichondria Panicea

This protocol generates sponges (Halichondria panicea) with a disturbed microbiome under controlled experimental conditions, in order to study bacterial recolonization dynamics. Bacteria-bacteria interactions can be analysed with this set-up within the host environment aiming at a better understanding of sponge-microbe symbiosis in vivo. It is divided into the sections 1) preparation, 2) antibiotic treatment and recovery phase, 3) recolonization with the natural microbiome and 4) sampling.

scholarly journals An in-vitro assay using human spermatozoa to detect toxicity of biologically active substances

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Tino Vollmer ◽  
Börje Ljungberg ◽  
Vera Jankowski ◽  
Joachim Jankowski ◽  
Griet Glorieux ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Toxicity Testing ◽  
Biologically Active ◽  
Human Spermatozoa ◽  
Vitro Assay ◽  
Cellular Behavior ◽  
Novel Method ◽  
Set Up

Abstract Identifying the key toxic players within an in-vivo toxic syndrome is crucial to develop targeted therapies. Here, we established a novel method that characterizes the effect of single substances by means of an ex-vivo incubation set-up. We found that primary human spermatozoa elicit a distinct motile response on a (uremic) toxic milieu. Specifically, this approach describes the influence of a bulk toxic environment (uremia) as well as single substances (uremic toxins) by real-time analyzing motile cellular behavior. We established the human spermatozoa-based toxicity testing (HSTT) for detecting single substance-induced toxicity to be used as a screening tool to identify in-vivo toxins. Further, we propose an application of the HSTT as a method of clinical use to evaluate toxin-removing interventions (hemodialysis).

High performance multifunctional green Co3O4 spinel nanoparticles: photodegradation of textile dye effluents, catalytic hydrogenation of nitro-aromatics and antibacterial potential

2017 ◽  
Vol 16 (5) ◽  
pp. 766-778 ◽  
Author(s):  
S. K. Jesudoss ◽  
J. Judith Vijaya ◽  
P. Iyyappa Rajan ◽  
K. Kaviyarasu ◽  
M. Sivachidambaram ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Catalytic Hydrogenation ◽  
High Performance ◽  
Punica Granatum ◽  
Seed Extract ◽  
Textile Dye ◽  
Nitro Aromatics ◽  
Pomegranate Seed ◽  
Antibacterial Potential ◽  
Spinel Nanoparticles

The multifunctional catalytic activity of Co3O4 spinel nanoparticles, synthesized using Punica granatum (pomegranate) seed extract was evaluated for its multiple applications.

scholarly journals Characterization of constituents, quality and stability of pomegranate seed oil (Punica granatum L.)

2016 ◽  
Vol 36 (1) ◽  
pp. 132-139 ◽  
Author(s):  
Illana Louise Pereira de MELO ◽  
Eliane Bonifácio Teixeira de CARVALHO ◽  
Ana Mara de Oliveira e SILVA ◽  
Luciana Tedesco YOSHIME ◽  
José Augusto Gasparotto SATTLER ◽  
...  
Keyword(s):  
Seed Oil ◽  
Punica Granatum ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed ◽  
Punica Granatum L
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