scholarly journals Terrestrial Microorganisms: Cell Factories of Bioactive Molecules with Skin Protecting Applications

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1836 ◽  
Author(s):  
Laure-Anne Peyrat ◽  
Nikolaos Tsafantakis ◽  
Katerina Georgousaki ◽  
Jamal Ouazzani ◽  
Olga Genilloud ◽  
...  
Keyword(s):  
Low Cost ◽  
Bioactive Molecules ◽  
Added Value ◽  
Pyrrole Derivatives ◽  
Cell Factories ◽  
Enhanced Production ◽  
Different Types ◽  
Terrestrial Environments ◽  
Whitening Activity ◽  
Competition For Resources

It is well known that terrestrial environments host an immense microbial biodiversity. Exposed to different types of stress, such as UV radiation, temperature fluctuations, water availability and the inter- / intra-specific competition for resources, terrestrial microorganisms have been evolved to produce a large spectrum of bioactive molecules. Bacteria, archaea, protists, fungi and algae have shown a high potential of producing biomolecules for pharmaceutical or other industrial purposes as they combine a sustainable, relatively low-cost and fast-production process. Herein, we provide an overview of the different bioactive molecules produced by terrestrial microorganisms with skin protecting applications. The high content in polyphenolic and carotenoid compounds produced by several strains, as well as the presence of exopolysaccharides, melanins, indole and pyrrole derivatives, mycosporines, carboxylic acids and other molecules, are discussed in the context of their antioxidant, photo-protective and skin-whitening activity. Relevant biotechnological tools developed for the enhanced production of high added value natural products, as well as the protecting effect of some antioxidant, hydrolytic and degrading enzymes are also discussed. Furthermore, we describe classes of microbial compounds that are used or have the potential to be used as antimicrobials, moisturizers, biosurfactants, pigments, flavorings and fragrances.

scholarly journals Exploration of the Potential Bioactive Molecules of Tamarillo (Cyphomandra betacea): Antioxidant Properties and Prebiotic Index

2021 ◽  
Vol 11 (23) ◽  
pp. 11322
Author(s):  
Verónica Reyes-García ◽  
Alfonso Totosaus ◽  
Lourdes Pérez-Chabela ◽  
Zaida Nelly Juárez ◽  
Gabriel Abraham Cardoso-Ugarte ◽  
...  
Keyword(s):  
Low Cost ◽  
Scientific Information ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Good Alternative ◽  
Bioactive Molecules ◽  
Added Value ◽  
Total Phenolic ◽  
Prebiotic Activity ◽  
Chemical Structures

Tamarillo is an alternative for the consumption of food with high added value through various technological methodologies with nutritional quality and low cost, generating an economic impact on society. The objective of this research was to evaluate the potential of tamarillo red variety, as a source of bioactive compounds, to generate scientific information on the importance of its chemical composition and antioxidant and prebiotic properties. Different analyses were carried out: spectroscopic methods (IR, UV, NMR) of pulp flour and epicarp flour, antioxidant properties, prebiotic activity, and bromatological analysis. The spectra obtained by FTIR, UV, and NMR allowed the identification of chemical structures associated with the inulin-like functional groups. Pulp flour showed the highest prebiotic activity with values of 1.49 for Lactiacidbacillus. plantarum. Total phenolic compounds content in epicarp flour was 206.23 mg/100 g dry weight, with an acceptable antioxidant property (ABTS+ = 6.27 TEAC and DPPH= %AA of 91.74 at a concentration of 250.00 µg/mL, 131.26 of IC50 ascorbic acid). The results regarding tamarillo as a source of bioactive molecules with important physiological properties as an antioxidant and putative prebiotic indicate it is a good alternative for the formulation of functional foods.

scholarly journals Phenolic Compounds From Brewer's Spent Grains: Toward Green Recovery Methods and Applications in the Cosmetic Industry

2021 ◽  
Vol 5 ◽  
Author(s):  
Rodrigo Macias-Garbett ◽  
Sergio Othón Serna-Hernández ◽  
Juan Eduardo Sosa-Hernández ◽  
Roberto Parra-Saldívar
Keyword(s):  
Phenolic Compounds ◽  
Low Cost ◽  
Antioxidant Properties ◽  
Economic Feasibility ◽  
Bioactive Molecules ◽  
Added Value ◽  
Anticancer Activities ◽  
Cosmetic Industry ◽  
Potential Applications ◽  
Pharmaceutical Industries

Brewers' spent grain (BSG) is the main by-product derived from the brewing industry, where it accounts for 85% of the total waste generated. The total annual production worldwide of this waste is 39 million tons. This lignocellulosic material is traditionally used as cattle feed and sold at a low retail price (~USD 45.00 per ton). However, efforts for the revalorization of this by-product are emerging since research has established that it can be used as a low-cost source of bioactive molecules and commodity chemicals that can bring value to integral biorefinery ventures. Among commodities, phenolic compounds have attracted attention as added-value products due to their antioxidant properties with applications in the food, cosmetic, and pharmaceutical industries. These phytochemicals have been associated with antiaging and anticancer activities that have potential applications on cosmetic products. This mini-review summarizes the most relevant extraction techniques used for the recovery of phenolic compounds from BSG while discussing their advantages and shortcomings and the potential applications from BSG bioactive extracts in the cosmetic industry and their reported beneficial effects. This mini-review also makes a brief comment on the role of phenolic compounds extraction in the economic feasibility of an integral BSG biorefinery.

scholarly journals Towards Iron-Titanium Oxide Nanostructures from Ecuadorian Black Mineral Sands

Minerals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 122
Author(s):  
Karina J. Lagos ◽  
Bojan A. Marinkovic ◽  
Alexis Debut ◽  
Karla Vizuete ◽  
Víctor H. Guerrero ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Titanium Oxide ◽  
Low Cost ◽  
Mineral Resources ◽  
Added Value ◽  
Secondary Phases ◽  
Hydrothermal Route ◽  
Oxide Nanostructures ◽  
Transmission Electron ◽  
Pre Treatment

Ecuadorian black mineral sands were used as starting material for the production of iron-titanium oxide nanostructures. For this purpose, two types of mineral processing were carried out, one incorporating a pre-treatment before conducting an alkaline hydrothermal synthesis (NaOH 10 M at 180 °C for 72 h), and the other prescinding this first step. Nanosheet-assembled flowers and nanoparticle agglomerates were obtained from the procedure including the pre-treatment. Conversely, nanobelts and plate-like particles were prepared by the single hydrothermal route. The nanoscale features of the product morphologies were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses. The ilmenite and hematite molar fractions, within the ilmenite-hematite solid solution, in the as-synthetized samples were estimated by Brown’s approach using the computed values of unit-cell volumes from Le Bail adjustments of X-ray powder diffraction (XRPD) patterns. The resulting materials were mainly composed of Fe-rich ilmenite-hematite solid solutions (hematite molar contents ≥0.6). Secondary phases, which possibly belong to lepidocrocite-like or corrugated titanate structures, were also identified. The current study demonstrated the feasibility of employing Ecuadorian mineral resources as low-cost precursors to synthesize high-added-value nanostructures with promising applications in several fields.

scholarly journals Extraction of Total Anthocyanins from Sicana odorifera Black Peel Fruits Growing in Paraguay for Food Applications

2021 ◽  
Vol 11 (13) ◽  
pp. 6026
Author(s):  
Laura Mereles ◽  
Silvia Caballero ◽  
Alberto Burgos-Edwards ◽  
Macarena Benítez ◽  
Danya Ferreira ◽  
...  
Keyword(s):  
Bioactive Molecules ◽  
Added Value ◽  
Total Anthocyanin ◽  
Efficient System ◽  
Natural Colorant ◽  
Solid Ratio ◽  
Screening Design ◽  
Total Anthocyanins ◽  
Food Applications ◽  
Antioxidant Characteristics

Sicana odorifera is a native fruit of South America large in size. Its dark-colored skin constitutes a useful byproduct for obtaining bioactive molecules because it is rich in polyphenolic compounds, such as anthocyanins. Obtaining appropriate extracts for obtaining anthocyanins can be useful for multiple applications in the food industry or for obtaining phytopharmaceuticals. In this work, the fruit and its peel composition were evaluated, an anthocyanin extraction system was designed and optimized, and the extract obtained was characterized. The peel composition of S. odorifera ripe fruits from the black accession growing in Paraguay was rich in polyphenol compounds and anthocyanins. Ultrasound-assisted extractions of total anthocyanins were studied, and the extraction variables were optimized. First, a screening design was considered to analyze time, pH, liquid–solid ratio, solvent concentration, and temperature. From the screening design, the significant variables were considered in a Box–Behnken design, and a response surface methodology was applied. The resulting total anthocyanin extract was characterized by UPLC-DAD-MS / MS-ESI. The most efficient system for the extraction of anthocyanins from the peel was at 15 min, 20 °C, pH = 6, 60% ethanol, and 80 mL/g of liquid–solid ratio. The highest concentration obtained was 61.908 mg C3G/g peel extract. In the extract, 12 main compounds were tentatively identified, including five anthocyanin derivatives, five flavonol derivatives, and two flavonol aglycones. This study provides information for the obtention of an anthocyanin-based dye from black kurugua peel, possibly useful for future applications as a natural colorant in high-added-value foods due to its antioxidant characteristics.

scholarly journals Photocatalytic three-component asymmetric sulfonylation via direct C(sp3)-H functionalization

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shi Cao ◽  
Wei Hong ◽  
Ziqi Ye ◽  
Lei Gong
Keyword(s):  
Asymmetric Catalysis ◽  
Organic Synthesis ◽  
Photochemical Reaction ◽  
Low Cost ◽  
Bioactive Molecules ◽  
Atom Economy ◽  
Mild Conditions ◽  
Wide Range ◽  
Unsaturated Carbonyl Compound ◽  
Direct Functionalization

AbstractThe direct and selective C(sp3)-H functionalization of cycloalkanes and alkanes is a highly useful process in organic synthesis owing to the low-cost starting materials, the high step and atom economy. Its application to asymmetric catalysis, however, has been scarcely explored. Herein, we disclose our effort toward this goal by incorporation of dual asymmetric photocatalysis by a chiral nickel catalyst and a commercially available organophotocatalyst with a radical relay strategy through sulfur dioxide insertion. Such design leads to the development of three-component asymmetric sulfonylation involving direct functionalization of cycloalkanes, alkanes, toluene derivatives or ethers. The photochemical reaction of a C(sp3)-H precursor, a SO2 surrogate and a common α,β-unsaturated carbonyl compound proceeds smoothly under mild conditions, delivering a wide range of biologically interesting α-C chiral sulfones with high regio- and enantioselectivity (>50 examples, up to >50:1 rr and 95% ee). This method is applicable to late-stage functionalization of bioactive molecules, and provides an appealing access to enantioenriched compounds starting from the abundant hydrocarbon compounds.

Glycans in scaffold design in tissue reconstruction

2021 ◽  
pp. 088391152199784
Author(s):  
Nipun Jain ◽  
Shashi Singh
Keyword(s):  
Cell Attachment ◽  
Low Cost ◽  
Growth Conditions ◽  
Downstream Signaling ◽  
Cell Carrier ◽  
Wide Range ◽  
Proliferation And Differentiation ◽  
Different Types ◽  
Tissue Reconstruction ◽  
A Cell

Development of an artificial tissue by tissue engineering is witnessed to be one of the long lasting clarified solutions for the damaged tissue function restoration. To accomplish this, a scaffold is designed as a cell carrier in which the extracellular matrix (ECM) performs a prominent task of controlling the inoculated cell’s destiny. ECM composition, topography and mechanical properties lead to different types of interactions between cells and ECM components that trigger an assortment of cellular reactions via diverse sensing mechanisms and downstream signaling pathways. The polysaccharides in the form of proteoglycans and glycoproteins yield better outcomes when included in the designed matrices. Glycosaminoglycan (GAG) chains present on proteoglycans show a wide range of operations such as sequestering of critical effector morphogens which encourage proficient nutrient contribution toward the growing stem cells for their development and endurance. In this review we discuss how the glycosylation aspects are of considerable importance in everyday housekeeping functions of a cell especially when placed in a controlled environment under ideal growth conditions. Hydrogels made from these GAG chains have been used extensively as a resorbable material that mimics the natural ECM functions for an efficient control over cell attachment, permeability, viability, proliferation, and differentiation processes. Also the incorporation of non-mammalian polysaccharides can elicit specific receptor responses which authorize the creation of numerous vigorous frameworks while prolonging the low cost and immunogenicity of the substance.

scholarly journals The Bioactive Potential of Trawl Discard: Case Study from a Crinoid Bed Off Blanes (North-Western Mediterranean)

Marine Drugs ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 83
Author(s):  
Alfredo García-de-Vinuesa ◽  
Montserrat Demestre ◽  
Arnau Carreño ◽  
Josep Lloret
Keyword(s):  
Bioactive Compounds ◽  
Fish Habitat ◽  
Antibacterial Properties ◽  
Marine Species ◽  
Western Mediterranean ◽  
Bioactive Molecules ◽  
Added Value ◽  
Future Research ◽  
Trawl Survey ◽  
Bioactive Potential

Although knowledge of the bioactive compounds produced by species inhabiting coastal waters is increasing, little is known about the bioactive potential produced by marine species occupying deeper habitats with high biodiversity and productivity. Here, we investigate about the bioactive potential of molecules produced by species that inhabit the crinoid beds, a poorly known essential fish habitat affected by trawling, wherein large amounts of commercial and noncommercial species are discarded. Based on a trawl survey conducted in 2019, 14% of the 64 species discarded on crinoid beds produce molecules with some type of bioactive potential, including; soft corals (Alcyonium palmatum); tunicates (Ascidia mentula); bony fish, such as horse mackerel (Trachurus trachurus); European hake (Merluccius merluccius); and chondrichthyans, such as small-spotted catshark (Scyliorhinus canicula). In addition, 16% of the discarded species had congeneric species that produce compounds with bioactive potential, indicating that such species might also possess similar types of bioactive molecules. Molecules with antioxidant, antitumour, antihypertensive, and antibacterial properties were the most frequent, which could provide the basis for future research aiming to discover new marine-based drugs and compounds for other human uses. Among all species or genera that produce compounds with bioactive potential, 68% presented medium or high vulnerability to trawling. Results show that the discarded catch contains many species, which produce different bioactive compounds that represent an added-value resource. These results highlight the importance of manage properly crinoid beds, to ensure that species that produce molecules with bioactive potential inhabiting these habitats are protected.

scholarly journals A metabolic modeling approach reveals promising therapeutic targets and antiviral drugs to combat COVID-19

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fernando Santos-Beneit ◽  
Vytautas Raškevičius ◽  
Vytenis A. Skeberdis ◽  
Sergio Bordel
Keyword(s):  
Literature Search ◽  
Metabolic Modeling ◽  
Bioactive Molecules ◽  
Flux Balance ◽  
Triacsin C ◽  
Protein Palmitoylation ◽  
Positive Sense ◽  
Different Types ◽  
Balance Analysis ◽  
Docking Calculations

AbstractIn this study we have developed a method based on Flux Balance Analysis to identify human metabolic enzymes which can be targeted for therapeutic intervention against COVID-19. A literature search was carried out in order to identify suitable inhibitors of these enzymes, which were confirmed by docking calculations. In total, 10 targets and 12 bioactive molecules have been predicted. Among the most promising molecules we identified Triacsin C, which inhibits ACSL3, and which has been shown to be very effective against different viruses, including positive-sense single-stranded RNA viruses. Similarly, we also identified the drug Celgosivir, which has been successfully tested in cells infected with different types of viruses such as Dengue, Zika, Hepatitis C and Influenza. Finally, other drugs targeting enzymes of lipid metabolism, carbohydrate metabolism or protein palmitoylation (such as Propylthiouracil, 2-Bromopalmitate, Lipofermata, Tunicamycin, Benzyl Isothiocyanate, Tipifarnib and Lonafarnib) are also proposed.

scholarly journals Plasmid Replicons for the Production of Pharmaceutical-Grade pDNA, Proteins and Antigens by Lactococcus lactis Cell Factories

2021 ◽  
Vol 22 (3) ◽  
pp. 1379
Author(s):  
Sofia O.D. Duarte ◽  
Gabriel A. Monteiro
Keyword(s):  
Lactococcus Lactis ◽  
Recombinant Proteins ◽  
Plasmid Copy Number ◽  
Fermented Food ◽  
Added Value ◽  
Regulatory Sequences ◽  
Plasmid Copy ◽  
Mucosal Vaccination ◽  
Cell Factories ◽  
Plasmid Replicons

The Lactococcus lactis bacterium found in different natural environments is traditionally associated with the fermented food industry. But recently, its applications have been spreading to the pharmaceutical industry, which has exploited its probiotic characteristics and is moving towards its use as cell factories for the production of added-value recombinant proteins and plasmid DNA (pDNA) for DNA vaccination, as a safer and industrially profitable alternative to the traditional Escherichia coli host. Additionally, due to its food-grade and generally recognized safe status, there have been an increasing number of studies about its use in live mucosal vaccination. In this review, we critically systematize the plasmid replicons available for the production of pharmaceutical-grade pDNA and recombinant proteins by L. lactis. A plasmid vector is an easily customized component when the goal is to engineer bacteria in order to produce a heterologous compound in industrially significant amounts, as an alternative to genomic DNA modifications. The additional burden to the cell depends on plasmid copy number and on the expression level, targeting location and type of protein expressed. For live mucosal vaccination applications, besides the presence of the necessary regulatory sequences, it is imperative that cells produce the antigen of interest in sufficient yields. The cell wall anchored antigens had shown more promising results in live mucosal vaccination studies, when compared with intracellular or secreted antigens. On the other side, engineering L. lactis to express membrane proteins, especially if they have a eukaryotic background, increases the overall cellular burden. The different alternative replicons for live mucosal vaccination, using L. lactis as the DNA vaccine carrier or the antigen producer, are critically reviewed, as a starting platform to choose or engineer the best vector for each application.

scholarly journals Design and Implementation on Auto Test Set of an Airborne Radar

2013 ◽  
Vol 756-759 ◽  
pp. 489-492
Author(s):  
Fu Lu Jin ◽  
Yun Peng Li ◽  
Hong Rui Wang
Keyword(s):  
Software Design ◽  
Resource Sharing ◽  
Low Cost ◽  
Airborne Radar ◽  
Automatic Test ◽  
Test Set ◽  
Test Items ◽  
Different Types ◽  
And Performance ◽  
Test Process

To automatic test the function and performance of an airborne radar, changeable test adapter is adopted to implement the hardware and software design of the automatic test set of the antenna, transceiver and indicator of the radar based on AT89C52. Problems such as t the different types of interfaces, the various kinds of signals and the test of microwave signal are solved successfully and the objectives of resource sharing and automatic test are realized. The test software is designed by modular structure, and with the help of automatic test set hardware, the required test items of the radar system are experimented and the test process control succeeded. Experiment results show that the automatic test set performs steadily and the results meet the requirements of the airborne radar. The set has the advantages of intelligent, manageable and reducing artificial errors. It provides effective guarantees for radars maintenance, fault diagnosis and fault detection, and has a wide application prospect with low cost.

Sign in / Sign up

Export Citation Format

Share Document