scholarly journals Microfluidics-Based Fabrication of Cell-Laden Hydrogel Microfibers for Potential Applications in Tissue Engineering

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1633 ◽  
Author(s):  
Gen Wang ◽  
Luanluan Jia ◽  
Fengxuan Han ◽  
Jiayuan Wang ◽  
Li Yu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Double Layer ◽  
Umbilical Vein ◽  
Single Layer ◽  
Microfluidic System ◽  
Hydrogel Scaffolds ◽  
Potential Applications ◽  
One Step ◽  
Umbilical Vein Endothelial Cells

Fibrous hydrogel scaffolds have recently attracted increasing attention for tissue engineering applications. While a number of approaches have been proposed for fabricating microfibers, it remains difficult for current methods to produce materials that meet the essential requirements of being simple, flexible and bio-friendly. It is especially challenging to prepare cell-laden microfibers which have different structures to meet the needs of various applications using a simple device. In this study, we developed a facile two-flow microfluidic system, through which cell-laden hydrogel microfibers with various structures could be easily prepared in one step. Aiming to meet different tissue engineering needs, several types of microfibers with different structures, including single-layer, double-layer and hollow microfibers, have been prepared using an alginate-methacrylated gelatin composite hydrogel by merely changing the inner and outer fluids. Cell-laden single-layer microfibers were obtained by subsequently seeding mouse embryonic osteoblast precursor cells (MC3T3-E1) cells on the surface of the as-prepared microfibers. Cell-laden double-layer and hollow microfibers were prepared by directly encapsulating MC3T3-E1 cells or human umbilical vein endothelial cells (HUVECs) in the cores of microfibers upon their fabrication. Prominent proliferation of cells happened in all cell-laden single-layer, double-layer and hollow microfibers, implying potential applications for them in tissue engineering.

scholarly journals Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds

2015 ◽  
Vol 12 (110) ◽  
pp. 20150509 ◽  
Author(s):  
J. P. Cattalini ◽  
A. Hoppe ◽  
F. Pishbin ◽  
J. Roether ◽  
A. R. Boccaccini ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Release ◽  
Umbilical Vein ◽  
Burst Release ◽  
Potential Applications ◽  
Cell Property ◽  
Umbilical Vein Endothelial Cells

This work aimed to develop novel composite biomaterials for bone tissue engineering (BTE) made of bioactive glass nanoparticles (Nbg) and alginate cross-linked with Cu 2+ or Ca 2+ (AlgNbgCu, AlgNbgCa, respectively). Two-dimensional scaffolds were prepared and the nanocomposite biomaterials were characterized in terms of morphology, mechanical strength, bioactivity, biodegradability, swelling capacity, release profile of the cross-linking cations and angiogenic properties. It was found that both Cu 2+ and Ca 2+ are released in a controlled and sustained manner with no burst release observed. Finally, in vitro results indicated that the bioactive ions released from both nanocomposite biomaterials were able to stimulate the differentiation of rat bone marrow-derived mesenchymal stem cells towards the osteogenic lineage. In addition, the typical endothelial cell property of forming tubes in Matrigel was observed for human umbilical vein endothelial cells when in contact with the novel biomaterials, particularly AlgNbgCu, which indicates their angiogenic properties. Hence, novel nanocomposite biomaterials made of Nbg and alginate cross-linked with Cu 2+ or Ca 2+ were developed with potential applications for preparation of multifunctional scaffolds for BTE.

Bio-Electrospraying of Human Umbilical Vein Endothelial Cells with a Customized Multi-Hole Spinneret

2016 ◽  
Vol 705 ◽  
pp. 291-296 ◽  
Author(s):  
Ting Zhang ◽  
Yuan Yuan Liu ◽  
Hong Chen Yu ◽  
Shuai Li ◽  
Hai Ping Chen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Cell Morphology ◽  
Trypan Blue ◽  
Umbilical Vein ◽  
Engineering Applications ◽  
Human Umbilical Vein ◽  
Sulforhodamine B ◽  
Umbilical Vein Endothelial Cells

Bio-electrospraying (BES) is becoming an attractive tool for the delivery of cells into scaffolds for tissue engineering applications. In this study, we aimed to electrospray human umbilical vein endothelial cells (HUVECs) and improve the efficiency of BES by designing a new customized multi-hole spinneret. We demonstrated that the multi-hole spinneret could produce continuous and stable jets during BES, and the efficiency was increased by 5–7 times. Morphological observations, trypan blue and sulforhodamine B assays revealed that the HUVECs electrosprayed using the multi-hole spinneret remained viable and proliferated at a rate similar to that of the controls. Thus, the new multi-hole nozzle can considerably improve output for BES without affecting cell morphology, viability, and proliferation.

scholarly journals Ectopic transient overexpression of OCT-4 facilitates BMP4-induced osteogenic transdifferentiation of human umbilical vein endothelial cells

2020 ◽  
Vol 11 ◽  
pp. 204173142090920
Author(s):  
Seung Hyun L Kim ◽  
Seunghun S Lee ◽  
Inseon Kim ◽  
Janet Kwon ◽  
Song Kwon ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Cell Therapy ◽  
Bone Morphogenetic Protein ◽  
Umbilical Vein ◽  
Cell Types ◽  
Bone Morphogenetic Protein 4 ◽  
Morphogenetic Protein ◽  
Umbilical Vein Endothelial Cells

Limitation in cell sources for autologous cell therapy has been a recent focus in stem cell therapy and tissue engineering. Among various research advances, direct conversion, or transdifferentiation, is a notable and feasible strategy for the generation and acquirement of wanted cell source. So far, utilizing cell transdifferentiation technology in tissue engineering was mainly restricted at achieving single wanted cell type from diverse cell types with high efficiency. However, regeneration of a complete tissue always requires multiple cell types which poses an intrinsic complexity. In this study, enhanced osteogenic differentiation was achieved by transient ectopic expression of octamer-binding transcription factor 4 ( OCT-4) gene followed by bone morphogenetic protein 4 treatment on human umbilical vein endothelial cells. OCT-4 transfection and bone morphogenetic protein 4 treatment resulted in enhanced expression of osteogenic markers such as core-binding factor alpha 1, alkaline phosphatase, and collagen 1 compared with bone morphogenetic protein 4 treatment alone. Furthermore, we employed gelatin-heparin cryogel in cranial defect model for in vivo bone formation. Micro-computed tomography and histological analysis of in vivo samples showed that OCT-4 transfection followed by bone morphogenetic protein 4 treatment resulted in efficient transdifferentiation of endothelial cells to osteogenic cells. These results suggest that the combination of OCT-4 and bone morphogenetic protein 4 on endothelial cells would be a reliable multicellular transdifferentiation model which could be applied for bone tissue engineering.

Platelet adhesion to human umbilical vein endothelial cells cultured on anionic hydrogel scaffolds

Biomaterials ◽  
2007 ◽  
Vol 28 (10) ◽  
pp. 1752-1760 ◽  
Author(s):  
Yong Mei Chen ◽  
Masaru Tanaka ◽  
Jian Ping Gong ◽  
Kazunori Yasuda ◽  
Sadaaki Yamamoto ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Platelet Adhesion ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Hydrogel Scaffolds ◽  
Umbilical Vein Endothelial Cells ◽  
Cells Cultured

Osteoblastic alkaline phosphatase mRNA is stabilized by binding to vimentin intermediary filaments

2015 ◽  
Vol 396 (3) ◽  
pp. 253-260 ◽  
Author(s):  
Yvonne Schmidt ◽  
Martin Biniossek ◽  
G. Björn Stark ◽  
Günter Finkenzeller ◽  
Filip Simunovic
Keyword(s):  
Tissue Engineering ◽  
Alkaline Phosphatase ◽  
Endothelial Cells ◽  
Umbilical Vein ◽  
Luciferase Reporter ◽  
Reporter Assay ◽  
Sirna Transfection ◽  
The Stability ◽  
Umbilical Vein Endothelial Cells ◽  
Mrna Binding

Abstract Vascularization is essential in bone tissue engineering and recent research has focused on interactions between osteoblasts (hOBs) and endothelial cells (ECs). It was shown that cocultivation increases the stability of osteoblastic alkaline phosphatase (ALP) mRNA. We investigated the mechanisms behind this observation, focusing on mRNA binding proteins. Using a luciferase reporter assay, we found that the 3′-untranslated region (UTR) of ALP mRNA is necessary for human umbilical vein endothelial cells (HUVEC)-mediated stabilization of osteoblastic ALP mRNA. Using pulldown experiments and nanoflow-HPLC mass spectrometry, vimentin was identified to bind to the 3′-UTR of ALP mRNA. Validation was performed by Western blotting. Functional experiments inhibiting intermediate filaments with iminodipropionitrile and specific inhibition of vimentin by siRNA transfection showed reduced levels of ALP mRNA and protein. Therefore, ALP mRNA binds to and is stabilized by vimentin. This data add to the understanding of intracellular trafficking of ALP mRNA, its function, and have possible implications in tissue engineering applications.

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