scholarly journals Profiling of Seed Proteome in Pea (Pisum sativum L.) Lines Characterized with High and Low Responsivity to Combined Inoculation with Nodule Bacteria and Arbuscular Mycorrhizal Fungi

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1603 ◽  
Author(s):  
Mamontova ◽  
Afonin ◽  
Ihling ◽  
Soboleva ◽  
Lukasheva ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Soil Microorganisms ◽  
Molecular Mechanisms ◽  
High Efficiency ◽  
Arbuscular Mycorrhizal ◽  
Cellular Respiration ◽  
Food Protein ◽  
Pisum Sativum L ◽  
Productivity Gain ◽  
Seed Proteome

Legume crops represent the major source of food protein and contribute to human nutrition and animal feeding. An essential improvement of their productivity can be achieved by symbiosis with beneficial soil microorganisms—rhizobia (Rh) and arbuscular mycorrhizal (AM) fungi. The efficiency of these interactions depends on plant genotype. Recently, we have shown that, after simultaneous inoculation with Rh and AM, the productivity gain of pea (Pisum sativum L) line K-8274, characterized by high efficiency of interaction with soil microorganisms (EIBSM), was higher in comparison to a low-EIBSM line K-3358. However, the molecular mechanisms behind this effect are still uncharacterized. Therefore, here, we address the alterations in pea seed proteome, underlying the symbiosis-related productivity gain, and identify 111 differentially expressed proteins in the two lines. The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation, manifested by up-regulation of proteins involved in cellular respiration, protein biosynthesis, and down-regulation of late-embryogenesis abundant (LEA) proteins. In contrast, the low-EIBSM line K-3358 demonstrated lower levels of the proteins, related to cell metabolism. Thus, we propose that the EIBSM trait is linked to prolongation of seed filling that needs to be taken into account in pulse crop breeding programs. The raw data have been deposited to the ProteomeXchange with identifier PXD013479.

scholarly journals Unique transcriptome features of pea (Pisum sativum L.) lines with differing responses to beneficial soil microorganisms

2021 ◽  
Vol 19 (2) ◽  
pp. 131-141
Author(s):  
Alexey M. Afonin ◽  
Emma S. Gribchenko ◽  
Evgeny A. Zorin ◽  
Anton S. Sulima ◽  
Daria A. Romanyuk ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Mycorrhizal Fungi ◽  
Soil Microorganisms ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Arbuscular Mycorrhizal ◽  
Plant Responses ◽  
Single Nucleotide Variants ◽  
Interaction Efficiency

BACKGROUND: Garden pea (Pisum sativum L.) possesses the ability to form beneficial symbioses with various soil microorganisms. However, different pea cultivars, genotypes, and lines gain more or less benefit from these interactions, so the trait named efficiency of interaction with soil microorganisms (EIBSM) was suggested to describe this phenomenon. The molecular mechanisms underlying the manifestation of the EIBSM trait are not properly studied, and only few works focusing on plant responses to combined microbial preparations have been published to date. METHODS: Eight pea lines previously described as contrasting in manifestation of the EIBSM trait were grown in pots with soil under combined inoculation with nodule bacteria and arbuscular mycorrhizal fungi, and the transcriptome profiles of the whole root systems of the plants were investigated using 3'MACE RNA sequencing. RESULTS: The relatedness of the lines inferred from the analysis of transcripts SNVs (Single Nucleotide Variants) corresponded to the manifestation of the EIBSM trait: three high-EIBSM lines and three low-EIBSM lines formed two distinct clusters. Thus, the gene expression profiles were compared between these two clusters, which enabled identification of transcriptome signatures characteristic for each group. The lines previously described as high-EIBSM have lower symbiotic activity, and the expression levels of pathogen response genes were elevated compared to the lines with low EIBSM. CONCLUSION: This result suggests that the mechanism of high interaction efficiency may be connected to stricter host control of symbionts, allowing such plants to expend less on the symbioses.

scholarly journals Proteome Map of Pea (Pisum Sativum L.) Embryos Containing Different Amounts of Residual Chlorophylls

2018 ◽  
Author(s):  
Tatiana Mamontova ◽  
Elena Lukasheva ◽  
Gregory Mavropolo-Stolyarenko ◽  
Carsten Proksch ◽  
Tatiana Bilova ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Seed Protein ◽  
High Efficiency ◽  
Animal Feed ◽  
Intracellular Localization ◽  
Food Protein ◽  
Protein Patterns ◽  
Pisum Sativum L ◽  
Pea Seeds ◽  
Pea Seed

Due to low culturing costs and high seed protein contents, legumes represent the main global source of food protein. Pea (Pisum sativum L.) is one of the major economically important legume crops, impacting both animal feed and human nutrition. Therefore, the quality of pea seeds needs to be ensured in the context of sustainable crop production and nutritional efficiency. Obviously, changes in seed protein patterns might directly affect both of these aspects. Thus, here we address the pea seed proteome in more detail and provide, to the best of our knowledge, the most comprehensive annotation of the functions and intracellular localization of pea seed proteins. Accordingly, 1938 and 1989 non-redundant proteins were identified in yellow and green pea seeds, in total. Only 35 and 44 proteins, respectively, could be additionally identified after protamine sulfate precipitation (PSP) potentially indicating the high efficiency of our experimental workflow. In total 981 protein groups could be assigned to 34 functional classes, which were to a large extent differentially represented in yellow and green seeds. Closer analysis of these differences by processing of the data in KEGG and String databases revealed their possible relation to a higher metabolic status and reduced longevity of green seeds.

scholarly journals The seed proteome of pea (Pisum sativum L.) lines different in their response to beneficial microorganisms highlights the different strategies for seed production in plants

2020 ◽  
Author(s):  
A. M. Afonin ◽  
T. Mamontova ◽  
A. Soboleva ◽  
E. Lukasheva ◽  
A. S. Sulima ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Seed Production ◽  
Soil Microorganisms ◽  
Seed Filling ◽  
Pisum Sativum L ◽  
Beneficial Microorganisms ◽  
Filling Phase ◽  
Seed Proteome

The analysis of the seed proteome of pea lines contrasting in their responsiveness to inoculation with soil microorganisms revealed the mechanisms underlying the extension of the seed filling phase in highly responsive line K-8274.

scholarly journals Proteome Map of Pea (Pisum sativum L.) Embryos Containing Different Amounts of Residual Chlorophylls

2018 ◽  
Vol 19 (12) ◽  
pp. 4066 ◽  
Author(s):  
Tatiana Mamontova ◽  
Elena Lukasheva ◽  
Gregory Mavropolo-Stolyarenko ◽  
Carsten Proksch ◽  
Tatiana Bilova ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Crop Production ◽  
Seed Protein ◽  
High Efficiency ◽  
Animal Feed ◽  
Intracellular Localization ◽  
Food Protein ◽  
Protein Patterns ◽  
Pisum Sativum L ◽  
Pea Seed

Due to low culturing costs and high seed protein contents, legumes represent the main global source of food protein. Pea (Pisum sativum L.) is one of the major legume crops, impacting both animal feed and human nutrition. Therefore, the quality of pea seeds needs to be ensured in the context of sustainable crop production and nutritional efficiency. Apparently, changes in seed protein patterns might directly affect both of these aspects. Thus, here, we address the pea seed proteome in detail and provide, to the best of our knowledge, the most comprehensive annotation of the functions and intracellular localization of pea seed proteins. To address possible intercultivar differences, we compared seed proteomes of yellow- and green-seeded pea cultivars in a comprehensive case study. The analysis revealed totally 1938 and 1989 nonredundant proteins, respectively. Only 35 and 44 proteins, respectively, could be additionally identified after protamine sulfate precipitation (PSP), potentially indicating the high efficiency of our experimental workflow. Totally 981 protein groups were assigned to 34 functional classes, which were to a large extent differentially represented in yellow and green seeds. Closer analysis of these differences by processing of the data in KEGG and String databases revealed their possible relation to a higher metabolic status and reduced longevity of green seeds.

scholarly journals Proteome Map of Pea (Pisum Sativum L.) Embryos Containing Different Amounts of Residual Chlorophylls

2018 ◽  
Author(s):  
Tatiana Mamontova ◽  
Elena Lukasheva ◽  
Gregory Mavropolo-Stolyarenko ◽  
Carsten Proksch ◽  
Tatiana Bilova ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Seed Protein ◽  
High Efficiency ◽  
Animal Feed ◽  
Intracellular Localization ◽  
Food Protein ◽  
Protein Patterns ◽  
Pisum Sativum L ◽  
Pea Seeds ◽  
Pea Seed

Due to low culturing costs and high seed protein contents, legumes represent the main global source of food protein. Pea (Pisum sativum L.) is one of the major economically important legume crops, impacting both animal feed and human nutrition. Therefore, the quality of pea seeds needs to be ensured in the context of sustainable crop production and nutritional efficiency. Obviously, changes in seed protein patterns might directly affect both of these aspects. Thus, here we address the pea seed proteome in more detail and provide, to the best of our knowledge, the most comprehensive annotation of the functions and intracellular localization of pea seed proteins. Accordingly, 1938 and 1989 non-redundant proteins were identified in yellow and green pea seeds, in total. Only 35 and 44 proteins, respectively, could be additionally identified after protamine sulfate precipitation (PSP) potentially indicating the high efficiency of our experimental workflow. In total 981 protein groups could be assigned to 34 functional classes, which were to a large extent differentially represented in yellow and green seeds. Closer analysis of these differences by processing of the data in KEGG and String databases revealed their possible relation to a higher metabolic status and reduced longevity of green seeds.

scholarly journals LysM Receptor-Like Kinase LYK9 of Pisum Sativum L. May Regulate Plant Responses to Chitooligosaccharides Differing in Structure

2021 ◽  
Vol 22 (2) ◽  
pp. 711
Author(s):  
Irina V. Leppyanen ◽  
Olga A. Pavlova ◽  
Maria A. Vashurina ◽  
Andrey D. Bovin ◽  
Alexandra V. Dolgikh ◽  
...  
Keyword(s):  
Immune Response ◽  
Pisum Sativum ◽  
Binding Capacity ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Phytopathogenic Fungi ◽  
Plant Responses ◽  
Pisum Sativum L ◽  
Receptor Like Kinase ◽  
Pea Mutants

This study focused on the interactions of pea (Pisum sativum L.) plants with phytopathogenic and beneficial fungi. Here, we examined whether the lysin-motif (LysM) receptor-like kinase PsLYK9 is directly involved in the perception of long- and short-chain chitooligosaccharides (COs) released after hydrolysis of the cell walls of phytopathogenic fungi and identified in arbuscular mycorrhizal (AM) fungal exudates. The identification and analysis of pea mutants impaired in the lyk9 gene confirmed the involvement of PsLYK9 in symbiosis development with AM fungi. Additionally, PsLYK9 regulated the immune response and resistance to phytopathogenic fungi, suggesting its bifunctional role. The existence of co-receptors may provide explanations for the potential dual role of PsLYK9 in the regulation of interactions with pathogenic and AM fungi. Co-immunoprecipitation assay revealed that PsLYK9 and two proposed co-receptors, PsLYR4 and PsLYR3, can form complexes. Analysis of binding capacity showed that PsLYK9 and PsLYR4, synthesized as extracellular domains in insect cells, were able to bind the deacetylated (DA) oligomers CO5-DA–CO8-DA. Our results suggest that the receptor complex consisting of PsLYK9 and PsLYR4 can trigger a signal pathway that stimulates the immune response in peas. However, PsLYR3 seems not to be involved in the perception of CO4-5, as a possible co-receptor of PsLYK9.

scholarly journals Radial gradient of the mitochondrial ultrastructure in Pisum sativum L. roots

2020 ◽  
pp. 78-84
Author(s):  
V.O. Brykov ◽  
Keyword(s):  
Pisum Sativum ◽  
Oxygen Content ◽  
Root Surface ◽  
Gradual Decrease ◽  
Cellular Respiration ◽  
Mitochondrial Ultrastructure ◽  
Terrestrial Plants ◽  
Oxygen Utilization ◽  
Radial Gradient ◽  
Pisum Sativum L

Tissue hypoxia in roots of terrestrial plants in the environment saturated with oxygen is conditioned by the high tissue density and oxygen utilization during the cellular respiration, and it is followed by a gradual decrease in the oxygen concentration from the organ surface. We used this natural model on the example of the main roots of 5-day-old seedlings of Pisum sativum L. growing under well-aerated conditions to study the ultrastructure of mitochondria in tissue with reducing oxygen content. In the direction from the root surface to the endodermis, it was found a gradual increase in the size of mitochondria due to swelling and partial fusion of the organelles. The formation of one dominant invagination led to the appearance of cup-shaped organelles in inner cortex cell layers. Such successive changes in the structure of organelles were called the radial gradient of the mitochondrial ultrastructure under a gradual decrease in the cell oxygen content. It is suggested that the described transformations in the mitochondrial ultrastructure could be an unspecific response to conditions that limit their energy and / or metabolic functions.

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