scholarly journals Solvent Front Position Extraction with Semi-Automatic Device as a Powerful Sample Preparation Procedure Prior to Quantitative Instrumental Analysis

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1358 ◽  
Author(s):  
Anna Klimek-Turek ◽  
Kamila Jaglińska ◽  
Magdalena Imbierowicz ◽  
Tadeusz Dzido
Keyword(s):  
Sample Preparation ◽  
Automatic Device ◽  
Preparation Procedure ◽  
3D Printer ◽  
Adsorbent Layer ◽  
Solvent Front ◽  
Front Position ◽  
Classical Procedure ◽  
Sample Preparation Procedure ◽  
Prototype Device

The new prototype device is applied to the Solvent Front Position Extraction (SFPE) sample preparation procedure. The mobile phase is deposited onto the chromatographic plate adsorbent layer by the pipette, which is moved, according to programmed movement path, by a 3D printer mechanism. The application of the prototype device to SFPE procedure leads to the increased repeatability of the results and significant reduction of the analysis time in comparison to the classical procedure of chromatogram development. Additionally, the new equipment allows use procedures that are not possible to run using the classic chromatogram development. In this paper, the results of manual and semi-automatic sample preparation with SFPE are compared and the possible application of this prototype device is discussed.

scholarly journals Solvent front position extraction with semi-automatic device as a powerful sample preparation procedure to quantitatitation of tryptophan in human plasma

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Anna Klimek-Turek ◽  
Adam Chomicki ◽  
Emilia Fornal ◽  
Anna Pradiuch ◽  
Michał Hys ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Internal Standard ◽  
Automatic Device ◽  
Preparation Procedure ◽  
Solvent Front ◽  
Front Position ◽  
Relative Standard ◽  
Sample Preparation Procedure ◽  
Sample Purification

Abstract In the paper the results of the tryptophan determination in human plasma samples prepared with the novel Solvent Front Position Extraction (SFPE) technique are presented. The SFPE procedure is used for preparation of real biological sample for the first time. The results obtained using SFPE are compared with those using the classical sample preparation procedure. Under the optimal conditions, tryptophan and its internal standard were separated from other plasma compounds (matrix) as a small common zone/spot on a chromatographic plate using semiautomatic device equipped with moving pipet, which distributed developing solvent on the adsorbent layer. Tryptophan and the internal standard were evenly distributed within the small common zone from that the both substances were extracted and the solution obtained was transferred to quantitation with LC–MS and MS techniques. The determination results are satisfactory, the percentage values of relative error and RSD relative standard deviation do not exceed 5%. The procedure is characterized by simplicity, high analysis throughput, very good sample purification and seems to be easy applicable to other biological samples with these advantages mentioned.

scholarly journals Optimization of Adsorbent Layer Type and Developing Solvent in Coccidiostats Sample Preparation with Procedure of Solvent Front Position Extraction

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 6011
Author(s):  
Maciej Jan Rybicki ◽  
Anna Klimek-Turek ◽  
Tadeusz Henryk Dzido
Keyword(s):  
Sample Preparation ◽  
Preparation Method ◽  
Automatic Device ◽  
Common Disease ◽  
The European Union ◽  
Adsorbent Layer ◽  
Sample Preparation Method ◽  
Widespread Application ◽  
Solvent Front ◽  
Front Position

Coccidiostats are drugs used against coccidiosis, a common disease among breeding animals. Their widespread application leads to the appearance of their residues in food, which is potentially harmful for human health and life. The European Union has established limits of concentrations of these drugs in premixtures and food. Nowadays, there are many methods for monitoring coccidiostats’ presence in market products, but their frequent weakness is sample preparation. Solvent Front Position Extraction is a planar chromatography-based sample preparation method that allows for effective assay of samples with coccidiostats when coupled with LC-MS/MS. The purpose of this research was to find common conditions for the effective isolation of eight coccidiostats from biological sample components with both lower and higher retention than the substances of interest. The acquired results were used for effective isolation of monensin and salinomycin from the premixture samples and allowed for their quantitative determination. The application of a semi-automatic device for the development of chromatograms positively impacted the results, confirming the effectiveness of the method for determining coccidiostats in biological samples.

Experimental design for metal extraction from facial cosmetics samples and determination by square wave voltammetry

2018 ◽  
Vol 10 (44) ◽  
pp. 5327-5334 ◽  
Author(s):  
Lucas Vinicius de Faria ◽  
Thalles Pedrosa Lisboa ◽  
Rafael Arromba de Sousa ◽  
Gustavo Senra Gonçalves de Carvalho ◽  
Maria Auxiliadora Costa Matos ◽  
...  
Keyword(s):  
Experimental Design ◽  
Sample Preparation ◽  
Square Wave Voltammetry ◽  
Metal Extraction ◽  
Preparation Procedure ◽  
Square Wave ◽  
Microwave Assisted ◽  
Wave Voltammetry ◽  
Sample Preparation Procedure ◽  
Extraction Of Metals

An alternative microwave-assisted sample preparation procedure is proposed for the rapid, reliable and easy extraction of metals from facial cosmetics samples.

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