scholarly journals Efficient Conversion of Cane Molasses Towards High-Purity Isomaltulose and Cellular Lipid Using an Engineered Yarrowia lipolytica Strain in Fed-Batch Fermentation

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1228 ◽  
Author(s):  
Zhi-Peng Wang ◽  
Qin-Qing Wang ◽  
Song Liu ◽  
Xiao-Fang Liu ◽  
Xin-Jun Yu ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
High Purity ◽  
Batch Fermentation ◽  
Low Cost ◽  
Lipid Level ◽  
Corn Steep Liquor ◽  
Biodiesel Production ◽  
Fed Batch ◽  
Cane Molasses ◽  
Fed Batch Fermentation

: Cane molasses is one of the main by-products of sugar refineries, which is rich in sucrose. In this work, low-cost cane molasses was introduced as an alternative substrate for isomaltulose production. Using the engineered Yarrowia lipolytica, the isomaltulose production reached the highest (102.6 g L−1) at flask level with pretreated cane molasses of 350 g L−1 and corn steep liquor of 1.0 g L−1. During fed-batch fermentation, the maximal isomaltulose concentration (161.2 g L−1) was achieved with 0.96 g g−1 yield within 80 h. Simultaneously, monosaccharides were completely depleted, harvesting the high isomaltulose purity (97.4%) and high lipid level (12.2 g L−1). Additionally, the lipids comprised of 94.29% C16 and C18 fatty acids, were proved suitable for biodiesel production. Therefore, the bioprocess employed using cane molasses in this study was low-cost and eco-friendly for high-purity isomaltulose production, coupling with valuable lipids.

Optimized fed-batch fermentation of L-β-hydroxy isobutyric acid by Yarrowia lipolytica

1999 ◽  
Vol 20 (3) ◽  
pp. 189-193
Author(s):  
H. S. Kim ◽  
J. Y. Ju ◽  
J. H. Suh ◽  
C. S. Shin
Keyword(s):  
Yarrowia Lipolytica ◽  
Batch Fermentation ◽  
Isobutyric Acid ◽  
Fed Batch ◽  
Fed Batch Fermentation

scholarly journals Waste Conversion into a Sweetener—Development of an Innovative Strategy for Erythritol Production by Yarrowia lipolytica

2020 ◽  
Vol 12 (17) ◽  
pp. 7122
Author(s):  
Ludwika Tomaszewska-Hetman ◽  
Waldemar Rymowicz ◽  
Anita Rywińska
Keyword(s):  
Yarrowia Lipolytica ◽  
Crude Glycerol ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Culture Broth ◽  
Biodiesel Production ◽  
Innovative Strategy ◽  
Simple Medium ◽  
Low Cost Strategy ◽  
Steep Liquor

The study proposed the innovative low-cost strategy for erythritol production by Yarrowia lipolytica through developing a simple medium based on industrial waste by-products and a natural method for culture broth purification. Results obtained proved that corn steep liquor might successfully replace traditional sources of nitrogen and other nutrients without compromising activities of the enzymes responsible for erythritol production and its production level. As a consequence, a production process was performed where Y. lipolytica A-6 was able to produce 108.0 g/L of erythritol, with a production rate of 1.04 g/Lh and a yield of 0.45 g/g of the medium containing exclusively 220 g/L of crude glycerol derived from biodiesel production and 40 g/L of corn steep liquor. Moreover, a comparable concentration of erythritol (108.1 g/L) was obtained when a part of crude glycerol was exchanged for the crude fraction of fatty acids in the two-steps process. Next, the collected post-fermentation broths were used in the culture with Y. lipolytica Wratislavia K1 for natural purification. The process resulted in a high increase of erythritol selectivity from 72% to 97% and in the production of 22.0 g/L of biomass with 40.4% protein content, which enables its use as an attractive animal feedstuff.

Improvement of Yarrowia lipolytica lipase production by fed-batch fermentation

2008 ◽  
Vol 49 (2) ◽  
pp. 212-215 ◽  
Author(s):  
Patrick Fickers ◽  
Jacqueline Destain ◽  
Philippe Thonart
Keyword(s):  
Yarrowia Lipolytica ◽  
Batch Fermentation ◽  
Lipase Production ◽  
Fed Batch ◽  
Fed Batch Fermentation

Repeated fed-batch fermentation using biosensor online control for citric acid production by Yarrowia lipolytica

2011 ◽  
Vol 153 (3-4) ◽  
pp. 133-137 ◽  
Author(s):  
Lucie Moeller ◽  
Mario Grünberg ◽  
Andreas Zehnsdorf ◽  
Andreas Aurich ◽  
Thomas Bley ◽  
...  
Keyword(s):  
Citric Acid ◽  
Yarrowia Lipolytica ◽  
Acid Production ◽  
Batch Fermentation ◽  
Online Control ◽  
Citric Acid Production ◽  
Fed Batch ◽  
Fed Batch Fermentation

scholarly journals Dissolved-oxygen feedback control fermentation for enhancing β-carotene in engineered Yarrowia lipolytica

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Peng Jun Lv ◽  
Shan Qiang ◽  
Liang Liu ◽  
Ching Yuan Hu ◽  
Yong Hong Meng
Keyword(s):  
Dissolved Oxygen ◽  
Yarrowia Lipolytica ◽  
Batch Fermentation ◽  
Utilization Rate ◽  
Crabtree Effect ◽  
Fed Batch ◽  
Promising Strategy ◽  
Fed Batch Fermentation ◽  
Β Carotene ◽  
Gene Expression Levels

Abstract The DO-stat fed-batch fermentation was carried out to explore the volumetric productivity of β-carotene in engineered Yarrowia lipolytica C11 strain. Using DO-stat fed-batch fermentation, we achieved 94 g/L biomass and 2.01 g/L β-carotene. Both biomass and β-carotene were about 1.28-fold higher than that in fed-batch fermentation. The ATP, NADP+/NADPH, and gene expression levels of tHMG, GGS1, carRA, and carB were promoted as compared to that in fed-batch fermentation. As for as the kinetic parameters in DO-stat fed-batch fermentation, μm′, Yx/s′, and Yp/s′ was 0.527, 0.353, and 0.158, respectively. The μm′ was elevated 4.66-fold than that in fed-batch fermentation. These data illustrate that more dissolved oxygen increased the biomass. The Yx/s′ and Yp/s′ were increased 1.15 and 22.57-fold, which suggest that the DO-stat fed-batch fermentation reduced the Crabtree effect and improved the utilization rate of glucose. Therefore, DO-stat fed-batch fermentation is a promising strategy in the industrialized production of β-carotene.

1,3-Propanediol Fermentation with the by-Product Glycerol from Biodiesel Production by a Genetic Modified Klebsiella pneumoniae

2012 ◽  
Vol 512-515 ◽  
pp. 323-329 ◽  
Author(s):  
Hong Juan Liu ◽  
Yu Jie Zhou ◽  
Zhong Zhen Cai ◽  
Yan Sun ◽  
Jian An Zhang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Batch Fermentation ◽  
Crude Glycerol ◽  
Acid Synthesis ◽  
Biodiesel Production ◽  
Klebsiella Pneumonia ◽  
Lactic Acid Concentration ◽  
Fed Batch ◽  
Fed Batch Fermentation

Nowadays, the utilization of the by-product glycerol has become a common problem with the large amount of biodiesel production. This paper studied the conversion of the by-product glycerol of biodiesel production by Klebsiella pneumonia to 1,3-propanediol. The crude glycerol could be used directly without refining by a lactate dehydrogenase deficient K. pneumoniae and the lactic acid concentration was very low. In the fed-batch fermentation of 7L fermenter, 93.3g/l 1,3-propanediol was obtained and lactic acid concentration was just 2g/l. Analysis showed that D-type lactic acid synthesis was reduced obviously. The fermentation also was scaled up on the 42L fermenter. 1,3-propanediol and 2,3-Butanediol concentration reached 81.5g/l and 33.8g/l, respectively. No lactic acid was detected. The demonstration fermentation in 5000L fermenter was also successfully performed. The final 1,3-propanediol concentration and productivity was 87g/l and 1.2g/(lh). This work was considered useful to the integrated production of biodiesel and 1,3-propanediol.

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