scholarly journals Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1226 ◽  
Author(s):  
Andrei Mocan ◽  
Alina Diuzheva ◽  
Sabin Bădărău ◽  
Cadmiel Moldovan ◽  
Vasil Andruch ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Iridoid Glycosides ◽  
Deep Eutectic Solvent ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Extraction Solvent ◽  
Microwave Assisted ◽  
Pattern Determination ◽  
Natural Deep Eutectic Solvent

Background: Galium is a plant rich in iridoid glycosides, flavonoids, anthraquinones, and small amounts of essential oils and vitamin C. Recent works showed the antibacterial, antifungal, antiparasitic, and antioxidant activity of this plant genus. Methods: For the determination of the multicomponent phenolic pattern, liquid phase microextraction procedures were applied, combined with HPLC-PDA instrument configuration in five Galium species aerial parts (G. verum, G. album, G. rivale, G. pseudoaristatum, and G. purpureum). Dispersive Liquid–Liquid MicroExtraction (DLLME) with NaCl and NAtural Deep Eutectic Solvent (NADES) medium and Ultrasound-Assisted (UA)-DLLME with β-cyclodextrin medium were optimized. Results: The optimal DLLME conditions were found to be: 10 mg of the sample, 10% NaCl, 15% NADES or 1% β-cyclodextrin as extraction solvent—400 μL of ethyl acetate as dispersive solvent—300 μL of ethanol, vortex time—30 s, extraction time—1 min, centrifugation at 12000× g for 5 min. Conclusions: These results were compared with microwave-assisted extraction procedures. G. purpureum and G. verum extracts showed the highest total phenolic and flavonoid content, respectively. The most potent extract in terms of antioxidant capacity was obtained from G. purpureum, whereas the extract obtained from G. album exhibited the strongest inhibitory effect against tyrosinase.

scholarly journals Chemical composition and antioxidant activity of beebread, and its influence on the glioblastoma cell line (U87MG)

2013 ◽  
Vol 57 (2) ◽  
pp. 147-157 ◽  
Author(s):  
Renata Markiewicz-Żukowska ◽  
Sylwia K. Naliwajko ◽  
Emilia Bartosiuk ◽  
Justyna Moskwa ◽  
Valery Isidorov ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Cell Line ◽  
Phenolic Content ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Glioblastoma Cell Line ◽  
Glioblastoma Cell ◽  
Acid Fermentation

Abstract Beebread is processed pollen stored in the cells of the honeycomb, with the addition of various enzymes and honey, which undergoes lactic acid fermentation. Ethanolic extracts (EBBs) were obtained from three different samples of beebread from Poland. Assays were carried out for the determination of chemical composition (GC/MS), for the total phenolic content, and for the antioxidant and cytotoxic activities. The effects of beebread extracts (10, 20, 30, 50, 100 μg/mL) on the viability of the glioblastoma cell line (U87MG) were studied after 24 h, 48 h, and 72 h. Our results indicated a time-dependent inhibitory effect on the viability of U87MG cells treated EBB. The main inhibitory effect of EBB was observed after 72 h; EBB treatment decreased cell viability to 49 - 66%.

Sensitive determination of psychotropic drugs in urine samples using continuous liquid-phase microextraction with an extraction solvent lighter than water

2018 ◽  
Vol 42 (6) ◽  
pp. 4450-4456 ◽  
Author(s):  
Lida Haghnazari ◽  
Hamid Nomani ◽  
Nazir Fattahi ◽  
Kiomars Sharafi ◽  
Masoud Moradi
Keyword(s):  
Liquid Phase ◽  
Psychotropic Drugs ◽  
Urine Samples ◽  
Liquid Phase Microextraction ◽  
Extraction Solvent ◽  
Sensitive Determination ◽  
First Time

A novel extraction vessel was employed, for the first time, in continuous liquid-phase microextraction (CLPME) with an extraction solvent lighter than water for the extraction of psychotropic drugs from urine samples.

Microwave-assisted extraction for recovery of polyphenolic antioxidants from ripe mango (Mangifera indica L.) peel using lactic acid/sodium acetate deep eutectic mixtures

2019 ◽  
Vol 26 (1) ◽  
pp. 78-92 ◽  
Author(s):  
Chandra Bhushan T Pal ◽  
Girirajsinh C Jadeja
Keyword(s):  
Lactic Acid ◽  
Sodium Acetate ◽  
Mangifera Indica ◽  
Deep Eutectic Solvent ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Solid Ratio ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Mango Peel

The present study investigates recovery of polyphenolic compounds from ripe mango ( Mangifera indica L.) peel using deep eutectic solvents based on microwave-assisted extraction method. Lactic acid/sodium acetate/water (3:1:4) screened out from eight different types of deep eutectic solvent systems was used as extractant. A Box–Behnken design along with response surface methodology was applied to optimize the effect of microwave power (W), time (min), and liquid-to-solid ratio (mL g−1) on polyphenol extraction. The optimized conditions determined were power of 436.45 W, time of 19.66 min, and liquid-to-solid ratio of 59.82 mL g−1. Under the optimal conditions, the recovery of total phenolic content, ferric reducing antioxidant power, and 2,2-diphenyl-1-picrylhydrazyl scavenging activity was 56.17 mg gallic acid equivalent g−1 dw, 683.27 µmol ascorbic acid equivalent g−1 dw, and 82.64 DPPHsc%, respectively. High Performance Liquid Chromatography (HPLC) analysis revealed mangiferin as the prominent phenolic compound in the mango peel extracts. Microwave-assisted deep eutectic solvent extraction showed remarkable effects on the extraction efficiency of phenolic compounds as revealed from scanning electron microscopy analysis. Rancimat test results revealed that the oxidative stability almost doubled upon addition of purified mango peel extracts to the sunflower oil and thus paving way for the use of mango peel waste as a potential source of antioxidants.

scholarly journals Rapid Method for the Determination of Organochlorine Pesticides and PCBs in Fish Muscle Samples by Microwave-Assisted Extraction and Analysis of Extracts by GC-ECD

2010 ◽  
Vol 93 (6) ◽  
pp. 1987-1994 ◽  
Author(s):  
Angelika M Wilkowska ◽  
Marek Biziuk
Keyword(s):  
Organochlorine Pesticides ◽  
Fish Muscle ◽  
Food Samples ◽  
Fat Content ◽  
Clupea Harengus ◽  
Microwave Assisted Extraction ◽  
Extraction Solvent ◽  
Microwave Assisted ◽  
Assisted Extraction

Abstract A procedure for the multiresidue determination of organochlorine pesticides and polychlorinated biphenyls in fish muscle samples has been developed. The method is based on the microwave-assisted extraction (MAE) of food samples from an acetonitrilewater (95 5, v/v) mixture followed by SPE cleanup of the extracts and analysis by GC with an electron capture detector. MAE operational parameters, such as the extraction solvent, temperature, and time, were optimized with respect to the extraction efficiency of the target compounds from food samples with 1013 fat content. The chosen extraction technique allows reduction of the solvent consumption and extraction time when compared with methods already used. Acetonitrile is a good extraction solvent for low-fat matrixes (220 fat content), such as fish samples, because it does not significantly dissolve the highly polar proteins, salts, and sugars commonly found in food and gives high recoveries of a wide polarity range of analytes. For purification, SPE using LC-Florisil was shown to be sufficient for the removal of coextracted substances. Recoveries >78 with RSD values <15 were obtained for all compounds under the selected conditions. Method quantification limits were in the 510 g/kg range. The method was applied to the analysis of samples of herring (Clupea harengus) purchased at the local fish market. The method is rapid and reliable for the determination of organochlorine analytes in fish muscle.

scholarly journals Comparison of total phenolic and flavonoids contents in Malaysian propolis extract with two different extraction solvents

2019 ◽  
Vol 6 (2) ◽  
pp. 1-11
Author(s):  
Siti Umairah Mokhtar
Keyword(s):  
Colorimetric Method ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Propolis Extract ◽  
Extraction Solvent ◽  
Percentage Yield

This study is aimed to prepare propolis extracts by using two different solvents which are ethanol and water via maceration method and to evaluate the total phenolic and flavonoid components of the extract. Optimization of the extraction process was done by using different solvents and sample-to-solvent ratio. The ethanolic and water extracts were analysed for the determination of Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) by using Folin-Ciocalteu colorimetric method and Aluminimum chloride method, respectively. Based on sample-to-solvent ratio, it showed that 1:10 gave the highest percentage yield compared to other ratio for both solvents. Ethanolic extract resulted contains high phenolic and flavonoids content compared to water extract. The result showed that sample-to-solvent ratio 1:5 gave highest TPC and TFC in ethanolic extract which are 29.09 and 7.75 mg GAE/g, respectively. In conclusion, this study revealed that ethanol is the best extraction solvent to be used for propolis extraction compared to water. It is also demonstrated that higher volume of solvent does not leads to high values of TPC and TFC. The optimum extraction parameters and conditions are necessary to be obtained in order to extract high TPC and TFC.

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