scholarly journals Development and Validation of an Analytical Method Based on HPLC-ELSD for the Simultaneous Determination of Rosmarinic Acid, Carnosol, Carnosic Acid, Oleanolic Acid and Ursolic Acid in Rosemary

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 323 ◽  
Author(s):  
Penghui Li ◽  
Ailing Liu ◽  
Yinhua Li ◽  
Bin Yuan ◽  
Wenjun Xiao ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Analytical Method ◽  
Rosmarinic Acid ◽  
High Performance ◽  
High Sensitivity ◽  
Carnosic Acid ◽  
Effective Manner

The safety, efficacy and stability of natural antioxidants have been the focus of research in the food industry, with the aim of rapidly analyzing and controlling the quality of rosemary and its extracts, a novel analytical method involving high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for the simultaneous determination of rosmarinic acid, carnosol, carnosic acid, oleanolic acid and ursolic acid in rosemary. Chromatographic separation was conducted with gradient elution mode by using a Zorbax SB-C18 column (4.6 mm × 250 mm, 5 μm) with mobile phases of methanol and 0.6% acetic acid. The drift tube temperature of ELSD was 70 °C, and the pressure of nebulizer nitrogen gas was 40 Psi. The method developed has high sensitivity (with limits of detection from 1.3 to 8.6 μg/mL), acceptable linearity over the tested concentrations (with correlation coefficients from 0.991 to 0.999), good repeatability (with intra- and inter-day CV less than 3.1% for all analytes) and satisfactory accuracy (with recovery between 95.5% and 100.8%). The method has been demonstrated as a powerful tool for the functional ingredients analysis and quality control of rosemary and its extracts in a cost- and time-effective manner.

scholarly journals Simultaneous Analysis of Ursolic Acid and Oleanolic Acid in Guava Leaves Using QuEChERS-Based Extraction Followed by High-Performance Liquid Chromatography

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Chang Xu ◽  
Yiyi Liao ◽  
Chunyan Fang ◽  
Makoto Tsunoda ◽  
Yingxia Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
High Performance ◽  
Correlation Coefficients ◽  
Guava Leaves ◽  
Guava Leaf

In this paper, a novel method of QuEChERS-based extraction coupled with high-performance liquid chromatography has been developed for the simultaneous determination of ursolic acid (UA) and oleanolic acid (OA) in guava leaves. The QuEChERS-based extraction parameters, including the amount of added salt, vortex-assisted extraction time, and absorbent amount, and the chromatographic conditions were investigated for the analysis of UA and OA in guava leaves. Under the optimized conditions, the method showed good linearity over a range of 1–320 μg mL−1, with correlation coefficients above 0.999. The limits of detection of UA and OA were 0.18 and 0.36 μg mL−1, respectively. The intraday and interday precision were below 1.95 and 2.55%, respectively. The accuracies of the UA and OA determinations ranged from 97.4 to 111.4%. The contents of UA and OA in the guava leaf samples were 2.50 and 0.73 mg g−1, respectively. These results demonstrate that the developed method is applicable to the simultaneous determination of UA and OA in guava leaves.

scholarly journals Simultaneous Determination of Eight Synthetic Permitted and Five Commonly Encountered Nonpermitted Food Colors in Various Food Matrixes by High-Performance Liquid Chromatography

2010 ◽  
Vol 93 (5) ◽  
pp. 1503-1514 ◽  
Author(s):  
Sumita Dixit ◽  
Subhash K Khanna ◽  
Mukul Das
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
High Sensitivity ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Hplc Method ◽  
Food Category ◽  
Food Commodities ◽  
Food Colors

Abstract A simple and sensitive HPLC method has been developed for the simultaneous determination of eight permitted food colors and five commonly encountered nonpermitted colors in various food commodities, including sugar-, fat-, and starch-based food matrixes. The method uses a specific food category-based cleanup/treatment procedure before color extraction to avoid the interference of food matrixes, and to obtain the optimal color extraction. Analysis was performed on a reversed-phase C18 -Bondapak column with ammonium acetate and acetonitrile gradient elution as the mobile phase; a programmable max-specific visible detection was used to monitor colors to obtain the higher sensitivity and expanded scope needed for multicolor blends having diverse absorption maxima. All colors showed good linearity, with regression coefficients of 0.99740.9999. The LOD and LOQ values ranged from 0.01 to 0.12 mg/L, and from 0.04 to 0.83 mg/L or mg/kg, respectively. The intraday and interday precision tests produced good RSD values, and the recoveries from different food matrixes ranged from 82 to 104%. The method offers high sensitivity for analysis of a wide variety of food matrixes containing a broad scope of multicolor blends. Two nonpermitted colors, orange II and metanil yellow, were found. Also, a number of samples contained permitted colors at levels two-to seven-fold higher than those prescribed.

Serotonin and its N-Acetylated and Acidic Derivatives in Insect Brain as Determined by High-Performance Liquid Chromatography with Electrochemical Detection

1993 ◽  
Vol 28 (1) ◽  
pp. 16-24 ◽  
Author(s):  
R. Vieira ◽  
M. Aldegunde
Keyword(s):  
Analytical Method ◽  
High Performance ◽  
Amperometric Detection ◽  
High Sensitivity ◽  
Hplc Method ◽  
Insect Brain ◽  
Oxidative Deamination ◽  
Wide Range ◽  
Hydrodynamic Voltammetry

The determination of serotonin (5-HT), N-acetylserotonin (NAS) and 5-hydroxy-3-indoleacetic acid (5-HIAA) in single brains of two acridids (Paracinema tricolor and Oedipoda caerulescens) was accomplished using a HPLC method combined with amperometric detection. A hydrodynamic voltammetry approach was used to assess the identity of each peak by comparing the voltammograms of standards and those of samples. The analytical method gave satisfactory reproducibility and sensitivity, and detected levels of 5-HT, NAS and 5-HIAA as low as 29, 55 and 10 fmol, respectively. This high sensitivity together with the simplicity of sample processing make the present analytical method suitable for a wide range of studies concerning indoleamine analyses in the insect nervous system. In both acridids, 5-HT showed the largest quantities, while its derivatives occurred in extremely low amounts. The results suggest that N-acetylation of 5-HT is quantitatively preferred to oxidative deamination in both species (NAS levels were 4-fold those of 5-HIAA). The relative importance of each catabolic pathway is discussed as related to physiological and genetic aspects.

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