scholarly journals Phenolic Content and Antioxidant Activity in Trifolium Germplasm from Different Environments

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 298 ◽  
Author(s):  
Aldo Tava ◽  
Łukasz Pecio ◽  
Roberto Lo Scalzo ◽  
Anna Stochmal ◽  
Luciano Pecetti
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Chemical Structure ◽  
Dry Matter ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Adaptive Strategies ◽  
Phenolic Composition ◽  
Leaf Extracts ◽  
Plant Environment

Phenolics are important mediators in plant-environment interactions. The presence and concentration of phenolic compounds and their antioxidant activity were evaluated in leaves and flowers of a set of Trifolium species originating from contrasting environments encompassing lowland and mountain sites. The current germplasm proved a great reservoir of phenolic compounds, with different chemical structure and, possibly, diversified biological activity. Germplasm groups with specific phenolic composition were observed. In some cases, different patterns bore a taxonomic meaning. Lowland germplasm showed higher concentration of total phenolics in leaves than mountain accessions (50.30 vs. 34.19 mg/g dry matter (DM)), while the latter had higher concentration in flowers (114.16 vs. 57.44 mg/g DM). Outstanding concentration of isoflavones was observed in leaves of lowland germplasm (24.19 mg/g DM), and of both proanthocyanidins and flavonoids in flowers of mountain germplasm (53.81 and 56.62 mg/g DM, respectively). The pattern of phenolic composition in lowland and mountain germplasm was suggestive of different adaptive strategies. Three assays of antioxidant activity were tested, which were characterised by rather different reactivity towards phenolic composition. The scavenging activity was higher for leaf extracts of lowland germplasm, and for flower extracts of mountain germplasm. Besides identifying germplasm of interest, this study also suggested possible links between environmental factors and concentration and composition of phenolic compounds.

scholarly journals Phenolic composition and antioxidant activity of different parts of pistacia vera L.

2016 ◽  
Vol 6 (1) ◽  
pp. 658-664
Author(s):  
Raouia Boumaiza ◽  
Ahmed Snoussi ◽  
Tania Cirkovic-Velickovic ◽  
Nabiha Bouzouita
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Higher Plants ◽  
Variety Identification ◽  
Total Phenolic ◽  
Pistacia Vera ◽  
Phenolic Composition ◽  
Floral Buds

Phenolic compounds are widely distributed in almost all higher plants and are present in different tissues. The quality and quantity of phenolics are used as criteria for variety identification. The present study has been planned to determine the phenolic composition of Pistacia vera. Total phenolic content and concentration of flavonoids of eight different extracts, from the floral buds and leaves of Pistacia vera. (Anacardiaceae) were determined using spectrophotometric methods. The total phenolic content ranged from 401.64 to 513.77 (mg GAE/g DW). The total flavonoid concentrations varied from 8.28 to 67.94 mg CE/g. Antioxidant activity was assessed by three methods: DPPH°+, β-carotene and ABTS and determined respectively as IC50 (mg/mL), CAA and PI (%), all extract have shown to be endowed by a high antioxidant activity similar to that of BHT. Ethanolic extract of male floral buds showed the highest phenolic and flavonoid concentration and the strongest antioxidant activity. The significant linear correlation was confirmed between the values for the total phenolic content and antioxidant activity of plant extracts. Phenolic compounds were investigated by LTQ Orbitrap LC-MS analysis and revealed 17compounds split into 5 groups: Abscisic acids, phenolic acids and their derivatives, flavonols, flavanones, flavones, flavanols. The high contents of phenolic compounds indicated that these last contribute to the antioxidant activity. The Pistacia vera can be regarded as promising candidates for natural plant sources of antioxidants with high value.

Phenolic Composition and Antioxidant Activity of Leaves of Strawberry Tree (Arbutus unedo L.) Populations from Morocco

2021 ◽  
Author(s):  
Z. Ait lhaj ◽  
K. Taghzouti ◽  
A. Bouyahya ◽  
G. Diria ◽  
K. Bakhy ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phenolic Composition ◽  
Leaf Extracts ◽  
Arbutus Unedo ◽  
Scavenging Effect ◽  
Promising Source

This study was aimed at determining the phenolic composition and the antioxidant activity of leaves of Arbutus unedo populations belonging to seven Moroccan locations. Antioxidant activity is evaluated using two methods: scavenging effect on DPPH and scavenging effect on ABTS radicals. The Arbutus unedo leaf extracts displayed average values of 61.95 mg GAE, 3.60 mg QE, and 0.15 mg CE per g of dry weight (DW) in polyphenols, flavonoids, and tannins contents, respectively. All extracts exhibited a strong antioxidant effect on both assays, presenting mean IC50 values of 4.22 in DPPH assay and 4.86 μg/ml in ABTS assay. Significant variability (P < 0.0001) was showed among surveyed populations; samples from the Chefchaouen location (CH) was revealed the highest total phenolic content (107.67 mg GAE/g DW) and the strongest antioxidant activity (IC50 values of 0.80 and 1.56 μg/ml, in DPPH and ABTS tests, respectively). The phenolic profile achieved by HPLC-DAD-ESI/MS was dominated by gallic acid, Gallic acid glucose derivative, and Galloylquinic acid derivative. A significant negative correlation (P < 0.05) was observed between the total phenolic content and the antioxidant activity. These results provide that Arbutus unedo leaves should be considered as a promising source of natural antioxidants, and as ingredients in complimentary food and nutraceutical products.

scholarly journals Evaluation of the phenolic compounds and the antioxidant potentials of Vitex agnus-castus L. leaves and fruits

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ceren Kımna ◽  
Tuğçe Fafal
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Leaf Extract ◽  
Phenolic Content ◽  
Leaf Extracts ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Antioxidant Agents ◽  
Agnus Castus ◽  
Chaste Tree

Abstract Objectives In this study, we aim at deciphering the phenolic content of Vitex agnus-castus L. leaf and fruit extracts prepared with different methods and relate it to their antioxidant activity. Methods In this study, phenolic compounds and the antioxidant potential of the ethanol fruit and leaf extracts of V. agnus-castus L. (Chaste tree) were evaluated spectrometrically. Furthermore, selected polyphenols, i.e., chlorogenic acid and rutin, were determined by the HPLC-DAD method qualitatively and quantitatively. Results The results obtained from leaf and fruit extracts were compared with a commercial product (CP) containing the fruit extract of V. agnus-castus. Leaf extract was found to be richer in flavonoids when compared to the fruit counterparts. Accordingly, they also showed higher antioxidant activity. Conclusions Extracts prepared here can be considered as promising antioxidant agents for future therapeutic formulations.

scholarly journals Antioxidant and Antimicrobial Evaluation and Chemical Investigation of Rosa gallica var. aegyptiaca Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6498
Author(s):  
Ahmed S. Abdelbaky ◽  
Abir M. H. A. Mohamed ◽  
Salman S. Alharthi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Rosa Gallica

Rosa gallica var. aegyptiaca is a species of flowering plant belonging to the Rosaceae family that plays an important role as a therapeutic agent for the treatment of specific types of cancer, microbial infections, and diabetes mellitus. This work presents the first report on the evaluation of the antioxidant and antimicrobial potential along with the phytochemical analysis of Rosa gallica var. aegyptiaca leaves. Five leaf extracts of hexane, chloroform, methanol, hydromethanol 80%, and water were prepared. Assessment of antioxidant activity was carried out via DPPH radical scavenging assay. Antimicrobial activity against five foodborne pathogenic bacteria—including Listeria monocytogenes, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Salmonella enteritidis—and the fungus Candida albicans, was examined using the disc diffusion method. Total phenolic content and total flavonoid content were determined using the Folin–Ciocalteu reagent and aluminum chloride methods, respectively. Isolation, identification, and quantification of phenolic compounds were performed using HPLC-DAD analysis. Amongst the five leaf extracts that were investigated, hydromethanol 80% extract possessed the highest extraction yield, antioxidant activity, total phenolic content, and antimicrobial activity against all tested microbial strains. Moreover, this extract furnished six active phenolic compounds: gallic acid (1), (+) catechin (2), chlorogenic acid (3), (–) epicatechin (4), quercetin-3-O-α-d-(glucopyranoside) (5), and quercetin (6). This study provides an alternative utilization of R. gallica var. aegyptiaca leaves as a readily accessible source of natural antioxidants and antimicrobials in the food and pharmaceutical industries.

scholarly journals Effects of Water Deficit Irrigation on Phenolic Composition and Antioxidant Activity of Monastrell Grapes under Semiarid Conditions

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1301
Author(s):  
Eva P. Pérez-Álvarez ◽  
Diego S. Intrigliolo ◽  
María Pilar Almajano ◽  
Pilar Rubio-Bretón ◽  
Teresa Garde-Cerdán
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Deficit Irrigation ◽  
Phenolic Content ◽  
Negative Impact ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Vitis Vinifera L ◽  
Phenolic Composition

The high phenolic compound content of grapes makes them an important source of natural antioxidants, among other beneficial health properties. Vineyard irrigation might affect berry composition and quality. Regulated deficit irrigation (RDI) is a widely used strategy to reduce the possible negative impact of irrigation on grapes, improving grape composition and resulting in water savings. Monastrell grapevines (Vitis vinifera L.) grown in eastern Spain were subjected to two water regime strategies: rainfed (non-irrigation) and RDI. The content of anthocyanins, flavonols, flavanols, hydroxybenzoic and hydroxycinnamic acids, and stilbenes was determined by HPLC and was related with total phenolic content and three antioxidant activity methods (ABTS, DPPH, and ORAC). The study aimed to evaluate and compare the phenolic composition and antioxidant potential of Monastrell grapes. The rainfed regime concentrated grapes in terms of phenolic compounds. Thus, total content of anthocyanins, flavonols, flavanols, hydroxybenzoic acids, and total phenols were higher in the rainfed grapes than in the RDI ones. Besides, the rainfed grapes doubled their antioxidant potential with respect to the RDI grapes with the ORAC method. Total phenolic content and antioxidant activity by ORAC assay positively correlated with most of the total phenolic compounds analyzed. This study demonstrates how field practices can modulate final grape composition in relation to their antioxidant activity.

scholarly journals Phenolics content and antioxidant activity of three Sorbus species

2019 ◽  
pp. 15-21
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Phenolic Acids ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Sorbus Aucuparia ◽  
Phenolics Content ◽  
Highly Correlated ◽  
Sorbus Aria

The phenolic content and antioxidant activity of Sorbus aucuparia L., Sorbus aria (L.) Crantz and Sorbus austriaca (Beck) Hedlund leaves and fruit were investigated. The quantification of total phenolics, flavonoids and phenolic acids was performed using the Folin–Ciocalteu, Dowd and Arnow methods, respectively. The antioxidant activity of the extracts was evaluated using DPPH, TEAC and FRAP methods with Trolox as a standard. Leaves had a higher content of phenolic compounds and antioxidant activity than the fruits for all species. The highest content of phenolics (76.11 mg gallic acid equivalents (GAE)/g plant), flavonoids (15.86 mg rutin equivalents (RE)/g plant) and phenolic acids (44.54 mg caffeic acid equivalents (CAE)/g plant) was determined for S. austriaca leaves. Sorbus austriaca fruit had the highest content of phenolics (13.21 mg GAE/g plant), flavonoids (1.82 mg RE/g plant) and S. aucuparia fruit had the highest content of phenolic acids (9.05 mg CAE/g plant). The antioxidant activity was in the range: DPPH=38.42–274.52 μmol Trolox equivalents (TE)/g plant; TEAC=43.23–403.02 μmol TE/g plant; FRAP=47.13–706.96 μmol TE/g plant. The highest values of antioxidant activity were found for S. austriaca leaf and fruit extracts while the lowest values were determined for S. aucuparia leaves and S. aria fruit. The antioxidant activity was highly correlated with total phenolics and phenolic acids.

scholarly journals Impact of Abiotic Stresses (Nitrogen Reduction and Salinity Conditions) on Phenolic Compounds and Antioxidant Activity of Strawberries

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1044
Author(s):  
José L. Ordóñez-Díaz ◽  
Vanessa Cardeñosa ◽  
José M. Muñoz-Redondo ◽  
Federico Ferreres ◽  
Gema Pereira-Caro ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Abiotic Stresses ◽  
High Salinity ◽  
Phenolic Content ◽  
Fragaria X Ananassa ◽  
Total Phenolic ◽  
Saline Stress ◽  
Phenolic Composition ◽  
Phenolic Profile

This study evaluated the phenolic profile and the antioxidant capacity of strawberries (Fragaria x ananassa Duch., cv. Primoris) cultivated under reduction of nitrogen and adverse irrigation conditions (high salinity), such as those prevailing in Almeria (south-eastern Spain). The phenolic compound and anthocyanin profiles were analysed by HPLC-DAD-ESI/MSn, and the antioxidant activity. Nineteen phenolic compounds were quantified, mainly ellagitannins, anthocyanins, and flavan-3-ols. The total phenolic content ranged from 731 to 1521 mg/100 g of dried weight. The flavan-3-ols group compounds from the strawberries were positively affected by saline stress, especially the afz-(e)Catechin content in the first sampling. The reduction of nitrogen and the adverse irrigation conditions for the cultivation of strawberries (cv. Primoris) partially affected the phenolic composition, with the harvesting dates having a greater influence.

scholarly journals Influence of processing on the phenolic compounds of feijoa pulp (Feijoa sellowiana)

2020 ◽  
Vol 11 ◽  
Author(s):  
Ana Carolina Leme Castelucci ◽  
Nataly Maria Viva De Toledo ◽  
Fernanda Francetto Juliano ◽  
Paula Porrelli Moreira Da Silva ◽  
Marta Helena Fillet Spoto
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Principal Component ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Phenolic Composition ◽  
And Control

The effect of processing on potential changes of antioxidant activity (DPPH and ABTS), total phenolic content (TPC) and total flavonoids (FLAV) of feijoa pulp during storage was investigated. In addition, possible modifications in the phenolic composition of the pulp. The pulp was subjected to refrigeration (control, 5 °C), pasteurization (85 °C/5 min) and irradiation (2.0 kGy). TPC, FLAV, DPPH and ABTS were measured during 28 days, whereas the identification and quantification of phenolic compounds were conducted after processing. The results were submitted to principal component analysis (SAS 9.4). Pasteurization preserved TPC, DPPH and ABTS for 21 days, while in the irradiated samples and control, the values were decreased and FLAV were maintained at high levels. The profile of phenolic compounds was different for each sample with six compounds being identified.

scholarly journals Characterization of phenolic compounds and antioxidant properties of Glycyrrhiza glabra L. rhizomes and roots

RSC Advances ◽  
2015 ◽  
Vol 5 (34) ◽  
pp. 26991-26997 ◽  
Author(s):  
Natália Martins ◽  
Lillian Barros ◽  
Montserrat Dueñas ◽  
Celestino Santos-Buelga ◽  
Isabel C. F. R. Ferreira
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Glycyrrhiza Glabra ◽  
Phenolic Composition ◽  
Glycyrrhiza Glabra L

The present work aims to characterize and quantify the phenolic composition and to evaluate the antioxidant activity of Glycyrrhiza glabra L. (commonly known as licorice) rhizomes and roots.

scholarly journals Optimization of Ultrasound-Assisted Extraction via Sonotrode of Phenolic Compounds from Orange By-Products

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1120
Author(s):  
María del Carmen Razola-Diaz ◽  
Eduardo Jesús Guerra-Hernández ◽  
Celia Rodríguez-Pérez ◽  
Ana María Gómez-Caravaca ◽  
Belén García-Villanova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
High Performance ◽  
Phenolic Content ◽  
Orange Peel ◽  
Polar Compounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Quantitative Analyses ◽  
Ultrasound Assisted

Orange peel is the main by-product from orange juice industry. It is a known source of bioactive compounds, mostly phenolic compounds, and it has been widely studied for its healthy activities. Thus, this research focuses on the establishment of ultrasound-assisted extraction of phenolic compounds in orange peel using a sonotrode. For this purpose, a Box–Behnken design of 27 experiments was carried out with four independent factors—ratio ethanol/water (v/v), time (min), amplitude (%), and pulse (%). Quantitative analyses of phenolic compounds were performed and the antioxidant activity was measured by ABTS and DPPH methods. The validity of the experimental design was confirmed by ANOVA and the optimal sonotrode extraction conditions were obtained by response surface methodology (RSM). The extracts obtained in the established conditions were analyzed by High Performance Liquid Chromatography (HPLC) coupled to mass spectrometer detector and 74 polar compounds were identified. The highest phenolic content and antioxidant activity were obtained using 45/55 ethanol/water (v/v), 35 min, amplitude 90% (110 W), and pulse 100%. The established method allows an increment of phenolics recovery up to 60% higher than a conventional extraction. Moreover, the effect of drying on phenolic content was also evaluated.

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