scholarly journals Simultaneous Optimization for Ultrasound-Assisted Extraction and Antioxidant Activity of Flavonoids from Sophora flavescens Using Response Surface Methodology

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 112 ◽  
Author(s):  
Jing Zhou ◽  
Lincheng Zhang ◽  
Qinping Li ◽  
Weifeng Jin ◽  
Weiyan Chen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging ◽  
Methanol Concentration ◽  
Extraction Time ◽  
Sophora Flavescens ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

The ultrasonic-assisted extraction process and antioxidant activity of flavonoids from Sophora flavescens were investigated in this study. In order to optimize the extraction of flavonoids from Sophora flavescens, the influence of extraction time, methanol concentration, ultrasonic temperature, and solvent-to-material ratio was analyzed. Results showed that the extraction yields reached a maximum with the extraction time of 30 min, methanol concentration of 80%, temperature of 80 °C, and solvent-to-material ratio of 26 mL/g. The flavonoids were determined by HPLC, and the mean yields of trifolirhizin, formononetin, isoxanthohumol, maackiain, and kurarinone under the optimal conditions were 2.570, 0.213, 0.534, 0.797, and 3.091 mg/g, respectively. The evaluation of vitro antioxidant activity exhibited Sophora flavescens flavonoids had a strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical-scavenging ability with IC50 of 0.984 and 1.084 mg/g, respectively. These results indicate that ultrasonic-assisted extraction is an efficient approach for the selective extraction of flavonoids, and response surface methodology further optimized the extraction.

Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Moringa oleifera Leaves Using Response Surface Methodology

2021 ◽  
pp. 363-367
Author(s):  
Abdelhakim Benarima ◽  
Mohamed Nasreddine Raache ◽  
Moulay Rachid Kouadri ◽  
Yassine Belaiche ◽  
Salah Eddine Laouini
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Moringa Oleifera ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Moringa Oleifera Leaves ◽  
Ultrasonic Assisted Extraction

Moringa oleifera is considered one of the most useful plants in the world because it's rich in bioactive substances, which employing on modern medical treatment, also can be used for many fields: pharmaceutical, food and cosmetics purposes. In this study, the response surface methodology (RSM) based on a Box–Behnken design (BBD) was employed to optimize the extraction time (X1: 20–60 min), extraction temperature (X2: 15–45 °C) and solvent-solid ratio (X3: 5–7 ml/g), to obtain a high crude of flavonoids yield from Moringa oleifera Leaves by ultrasonic-assisted extraction technique (UAE). The optimum conditions were an extraction time 23 (min), extraction temperature 44 (°C) and solvent-solid ratio 5.05 (ml/g). Under these conditions, the experimental yield was 72.65 (mg ER/g), well matched with the predicted yield 74.34 (mg ER/g) with the coefficients of determination (R2= 0.9861), thus indicating the suitability of response surface methodology in optimizing the ultrasound-assisted extraction of flavonoids from Moringa oleifera Leaves.

scholarly journals Valorisation of Sea Buckthorn Pomace by Optimization of Ultrasonic-Assisted Extraction of Soluble Dietary Fibre Using Response Surface Methodology

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1330
Author(s):  
Shehzad Hussain ◽  
Minaxi Sharma ◽  
Rajeev Bhat
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Dietary Fibre ◽  
Sea Buckthorn ◽  
Extraction Time ◽  
Hydration Properties ◽  
Assisted Extraction ◽  
Soluble Dietary Fibre ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Sea buckthorn pomace is a valuable industrial waste/by-product obtained after juice production that contains bioactive, health-promoting dietary fibres. This pomace finds usage as animal feed or simply discarded, owed to the lack of appropriate handling or processing facilities. The present study was aimed to evaluate the effects of green extraction technologies such as ultrasonic-assisted extraction on the yield of soluble dietary fibre (SDF) from sea buckthorn pomace. Response surface methodology (RSM) coupled with Box–Behnken design (BBD) was applied for optimization of SDF yield. The effects of sonication temperature (60–80 °C), sonication power (100–130 W) and extraction time (30–60 min) on the yield of SDF were also investigated. Furthermore, colour measurement and hydration properties of sea buckthorn pomace powder (STP) and dietary fibre fractions (SDF and insoluble dietary fibre, IDF) were also investigated. From the RSM results, the optimal sonication temperature (67.83 °C), sonication power (105.52 W) and extraction time (51.18 min) were identified. Based on this, the modified optimum conditions were standardised (sonication temperature of 70 °C, sonication power of 105 W and extraction time of 50 min.). Accordingly, the yield of SDF obtained was 16.08 ± 0.18%, which was close to the predicted value (15.66%). Sonication temperature showed significant effects at p ≤ 0.01, while sonication power and extraction time showed significant effects at p ≤ 0.05 on the yield of SDF. The result on colour attributes of STP, SDF and IDF differed (L* (STP: 54.71 ± 0.72, IDF: 72.64 ± 0.21 and SDF: 54.53 ± 0.31), a* (STP: 52.35 ± 1.04, IDF: 32.85 ± 0.79 and SDF: 43.54 ± 0.03), b* (STP: 79.28 ± 0.62, IDF: 82.47 ± 0.19 and SDF: 71.33 ± 0.50), and ∆E* (STP: 79.93 ± 0.50, IDF: 74.18 ± 0.30 and SDF: 68.40 ± 0.39)). Higher values of hydration properties such as the water holding, swelling and oil holding capacities were found in SDF (7.25 ± 0.10 g g−1, 7.24 ± 0.05 mL g−1 and 1.49 ± 0.02 g g−1), followed by IDF (6.30 ± 0.02, 5.75 ± 0.07 and 1.25 ± 0.03) and STP (4.17 ± 0.04, 3.48 ± 0.06 and 0.89 ± 0.03), respectively. Based on our results, response surface methodology is recommended to be adopted to optimize the ultrasonic-assisted extraction to obtain maximum yield of SDF from sea buckthorn pomace. These results can be of practical usage while designing future functional food formulations using sea buckthorn pomace.

scholarly journals Optimization of Ultrasonic-Assisted Extraction by Response Surface Methodology with Maximal Phenolic Yield and Antioxidant Activity from Acer truncatum Leaves

2017 ◽  
Author(s):  
Lingguang Yang ◽  
Peipei Yin ◽  
Hang Fan ◽  
Qiang Xue ◽  
Ke Li ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Gallic Acid ◽  
Response Surface ◽  
Total Phenol ◽  
Phenolic Composition ◽  
Liquid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

This study was designed for the first time to improve phenolic yield and antioxidant activity of ultrasonic-assisted extraction from Acer truncatum leaves (ATL) using response surface methodology, and phenolic composition in ATL extracted under the optimized condition were characterized by UPLC-QTOF-MS/MS. Solvent and extraction time were selected based on preliminary experiments, and a four-factors-three-levels central composite design was conducted to optimize solvent concentration (X1), material-to-liquid ratio (X2), ultrasonic temperature (X3) and power (X4) for an optimal total phenol yield (Y1) and DPPH• antioxidant activity (Y2). The results showed that the optimal combination was ethanol: water (v:v) 66.21%, material-to-liquid ratio 1:15.31 g/mL, ultrasonic temperature 60 °C, power 267.30 W, and time 30 min with three extractions, giving a maximal total phenol yield of 7593.62 mg gallic acid equivalent /100 g d.w. and a maximal DPPH• antioxidant activity of 74241.61 μmol Trolox equivalent/100 g d.w.. Furthermore, 22 phenolics were first identified in ATL extract obtained under the optimized conditions, indicating that gallates, gallotannins, quercetin, myricetin and chlorogenic acid derivatives were the main phenolic composition in ATL. What’s more, a gallotannins pathway existing in ATL from gallic acid to penta-O-galloyl-glucoside was interpreted. All these results provided practical information aiming at full utilization of phenolics in ATL, together with fundamental knowledge for further research.

OPTIMIZATION OF ULTRASONIC ASSISTED EXTRACTION OF ANTIOXIDANTS FROM MORINGA OLEIFERA LAM. LEAVES USING RESPONSE SURFACE METHODOLOGY

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (12) ◽  
pp. 32-38
Author(s):  
H. N Sholapur ◽  
◽  
F. S Dasankoppa ◽  
Z. Abbas ◽  
N.G.N. Swamy
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Moringa Oleifera ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

The present study was embarked upon for the optimization of conditions aimed at ultrasonic assisted extraction (UAE) of antioxidant chemicals from Moringa oleifera leaves by applying response surface methodology (RSM) based on face centered central composite design (CCD). Evaluation was done at three levels (20 experimental designs) with three process variables: extraction temperature (20, 30, 40oC), extraction time (20, 30, 40 min) and liquid-solid ratio (20:1, 30:1, 40:1) using probe sonicator. The extraction process optimization based on RSM was focused on the capacity of antioxidants present in the extracts to scavenge the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) free radicals. To envisage the optimization process, multiple regression analysis was carried out using RSM tool to obtain a quadratic polynomial equation. Stastical analysis of the factors and their interactions were tested using analysis of variance (ANOVA). Extraction of antioxidants was mainly influenced by liquid-solid ratio and temperature. The ideal combination of variables designed by RSM for maximum radical-scavenging activity was optimized with extracting temperature of 39.80 °C, extracting time of 39.95 min and liquidsolid ratio of 24.00 mL/g to obtain a predicted value of 85.528% of DPPH radical-scavenging activity, which was very close to the experimentally value of 86.49 % with an error of 1.12%, indicating suitability of the model.

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