scholarly journals Isolation, Chemical Profile and Antimalarial Activities of Bioactive Compounds from Rauvolfia caffra Sond

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 39 ◽  
Author(s):  
Dorcas Tlhapi ◽  
Isaiah Ramaite ◽  
Teunis Van Ree ◽  
Chinedu Anokwuru ◽  
Taglialatela-Scafati Orazio ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Indole Alkaloids ◽  
Preparative Thin Layer Chromatography ◽  
Ultra Performance Liquid Chromatography ◽  
Antiplasmodial Activity ◽  
Liquid Chromatography Mass Spectrometry ◽  
Active Fraction ◽  
Chemical Profile ◽  
Layer Chromatography ◽  
Crude Methanol Extract

In this study, the chemical profile of a crude methanol extract of Rauvolfia caffra Sond was determined by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Column chromatography and preparative thin layer chromatography were used to isolate three indole alkaloids (raucaffricine, N-methylsarpagine and spegatrine) and one triterpenoid (lupeol). The antiplasmodial activity was determined using the parasite lactate dehydrogenase (pLDH) assay. The UPLC-MS profile of the crude extract reveals that the major constituents of R. caffra are raucaffricine (m/z 513.2) and spegatrine (m/z 352.2). Fraction 3 displayed the highest antiplasmodial activity with an IC50 of 6.533 μg/mL. However, raucaffricine, isolated from the active fraction did not display any activity. The study identifies the major constituents of R. caffra and also demonstrates that the major constituents do not contribute to the antiplasmodial activity of R. caffra.

scholarly journals ANTIBACTERIAL ACTIVITY OF FLAVONOID FROM KEPEL (STELECHOCARPUS BURAHOL) LEAVES AGAINST STAPHYLOCOCCUS EPIDERMIDIS

2017 ◽  
Vol 9 (10) ◽  
pp. 292
Author(s):  
Susi Indariani ◽  
Aisyah Hidayat ◽  
Latifah K. Darusman ◽  
Irmanida Batubara
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Functional Group ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Minimum Bactericidal Concentration ◽  
Preparative Thin Layer Chromatography ◽  
Active Fraction ◽  
Microdilution Method ◽  
Layer Chromatography

Objective: The objective of this research was to separate flavonoids of kepel (Stelechocarpus burahol) leaves from methanol extract as antibacterial agent against Staphylococcus epidermidis that have a role in body odor.Methods: The methanol extracts of kepel was fractionated in methanol: water (7:3), n-hexane and chloroform consecutively. Methanol: water (7:3) extract with the highest flavonoids content, fractioned by silica gel column chromatography (isocratic elution, with n-buthanol: methanol: acetic acid (1:8:1) as eluent) to produce 7 fractions. All fractions were tested for antibacterial activity with a microdilution method. The most active fractions was determined using UV-VIS spectrophotometer (Shimadzu, Japan) and FTIR (Brucker, Germany).Results: Fraction V was the most active fraction with minimum inhibitory concentration (MIC) 0.06 mg/ml and minimum bactericidal concentration (MBC) of 0.50 mg/ml. Fraction V was further separated by preparative thin layer chromatography (TLC) and gave three fractions. Fraction V3 was the most active fraction with MIC 1.00 mg/ml and MBC of 2.00 mg/ml. Identification of fraction V3 based on assessments on ultraviolet-visible and infrared spectrum showed the maximum wavelength at 327 nm. These results indicate a transition π → π * and n → π * generated from the chromophore conjugated C = C and C = O. Based on the allegations of the functional group obtained, then alleged that in Fraction V3 containing flavones compounds.Conclusion: These results suggest that flavonoid of S. burahol leaves extracts is potential as antibacterial agents against S. epidermidisand therefore justifies their usage in traditional medicine for the treatment of body odor. 

Isolation of the antidiarrhoeal tiliroside and its derivative from Waltheria indica leaf extract

2021 ◽  
Vol 25 (1) ◽  
pp. 86-92
Author(s):  
B.A. Ayinde ◽  
J.O. Owolabi ◽  
I.S. Uti ◽  
P.C. Ogbeta ◽  
M.I. Choudhary
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Preparative Thin Layer Chromatography ◽  
Inhibitory Effects ◽  
Aqueous Fraction ◽  
Vacuum Liquid Chromatography ◽  
Ileum Contraction ◽  
Layer Chromatography ◽  
Dose Dependent

The antidiarrhoeal effect of Waltheria indica methanol extract and fractions have been reported earlier but, the present work examined the intestinal relaxant effects of two flavonoid-phenyl propanoids isolated from the methanol extract. The active aqueous fraction was subjected to vacuum liquid chromatography using dichloromethane with increasing concentration of ethyl acetate, and that of methanol and water successively. The ten (10) fractions obtained were combined to give seven (7). The fraction 2 (C, D) was subjected to preparative thin layer chromatography on silica gel GF254 (10-40μm) using CHCl3-CH3OH (8:2) to obtain compound coded F2. Fraction 4 (F) was subjected to column chromatography using silica gel (60-120μm mesh) and eluted with  dichloromethane with increasing concentrations of methanol. Fractions 9-28 were combined and subjected to column  chromatography using chloroform with increasing concentration of methanol. The fractions 1-16 of these were purified on Sephadex LH-20 to obtain compound BAA. The identities of the two compounds were established using spectroscopic methods. The  antidiarrheal effect of compound F2 was evaluated on mice using charcoal transit (100,200, 400mg/kg), castor oil (40, 60 mg/kg)  while the two compounds were examined for their inhibitory effects on Ach-induced ileum contraction. The effects of the  compounds were compared with loperamide (3mg/kg) and atropine (80μg). Compounds F2 and BAA were identified as tiliroside and 3’’’, 5’’’-dimethoxy tiliroside respectively. Tiliroside inhibited the charcoal transition in the animals in a dose dependent pattern with 400mg/ mL eliciting 63.41% inhibition compared to 59.23% produced by loperamide. The compound also elicited significantly (P<0.05) prolonged onset of stooling and reduced the number and weight of stools produced lower than the control. The two  compounds drastically inhibited the Ach-induced contractions of the ileum. The compound, tiliroside at 10mg, completely abolished  the contraction by Ach unlike 3’’’, 5’’’-dimethoxy tiliroside which reduced the contraction to 1.92% at 20mg. The identified compounds seem to be responsible for the ethnomedicinal use of the plant in treating diarrhea.

scholarly journals Structural Elucidation of Irish Ale Bioactive Polar Lipids with Antithrombotic Properties

Biomolecules ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1075 ◽  
Author(s):  
Alexandros Tsoupras ◽  
Ronan Lordan ◽  
Eoin O'Keefe ◽  
Katie Shiels ◽  
Sushanta Kumar Saha ◽  
...  
Keyword(s):  
Inflammatory Mediator ◽  
Fatty Acid Content ◽  
Platelet Activating Factor ◽  
Preparative Thin Layer Chromatography ◽  
Polar Lipids ◽  
Liquid Chromatography Mass Spectrometry ◽  
Omega 3 ◽  
Human Platelet Aggregation ◽  
Layer Chromatography ◽  
By Products

The structures of bioactive polar lipids (PLs) of Irish ale with potent antithrombotic and cardioprotective properties were elucidated. Ale PL was fractionated by preparative thin layer chromatography (TLC) into subclasses, and their antithrombotic effect was assessed against human platelet aggregation induced by the pro-inflammatory mediator, platelet-activating factor (PAF). The fatty acid content and the overall structures of ale PL were elucidated by liquid chromatography mass spectrometry (LC-MS). Phosphatidylcholines (PC) and molecules of the sphingomyelin (SM) family exhibited the strongest anti-PAF effects, followed by phosphatidylethanolamines (PE). PC contained higher amounts of omega-3 polyunsaturated fatty acids (n-3 PUFA) and thus the lowest n-6/n-3 ratio. Bioactive diacyl and alkyl-acyl PC and PE molecules bearing n-3 PUFA at their sn-2 position, especially docosahexaenoic acid (DHA) and α-linolenic acid (ALA) but mostly oleic acid (OA), were identified in both PC and PE subclasses. Eicosapentaenoic acid (EPA) was present only in bioactive PC molecules and not in PE, explaining the lower anti-PAF effects of PE. Bioactive sphingolipid and glycolipid molecules with reported anti-inflammatory and anti-tumour properties, such as specific ceramides and glucosylcerebrosides with sphingosine, phytosphingosine and dihydrosphingosine bases but also specific monogalactodiglycerides and SM species bearing ALA at their sn-2 position, were identified in the SM subclass, providing a rational for its strong bioactivities against the PAF pathway. Further studies are required on the health benefits of bioactive PL from beer and brewery by-products.

scholarly journals Identification of 4-4’-(1-methylethylidene)-bisphenol from an Endophytic Fungus Cladosporium oxysporum derived from Aglaia odorata

2018 ◽  
Vol 14 (2) ◽  
pp. 193
Author(s):  
Armila Fatma Setyaningrum ◽  
Risma Pratiwi ◽  
Suciati Suciati ◽  
Noor Erma Nasution Sugijanto ◽  
Gunawan Indrayanto
Keyword(s):  
Escherichia Coli ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Preparative Thin Layer Chromatography ◽  
Chemical Constituent ◽  
Biologically Active ◽  
Raw Material ◽  
Economic Potential ◽  
Active Fraction ◽  
Layer Chromatography

<p>Endophytic fungi has an economic potential as raw material for biologically active compounds<em>. Cladosp</em><em>o</em><em>rium oxysporum</em><em> </em>is one of the endophytic fungi isolated from Indonesian medicinal plant <em>Aglaia odorata </em>Lour (Local name: Pacarcina). This planth has been used for fever, cough, diarrhea, inflammation and injury. In our previous study, the ethyl acetate extract and several fractions of the extract of <em>C. oxysporum </em>showed antimicrobial activity against <em>Candida albicans, Escherichia coli</em>, and <em>Staphylococcus aureus</em>. The objective of the currents study is to investigate the chemical constituent of the active fraction.<strong> </strong>Purification of the metabolite was achieved by using column chromatography followed by preparative thin layer chromatography. Identification of the metabolite was conducted by using TLC densitometry, GC-FID and GC-MS. Compound 1 was isolated from fraction 12. The purity of this compound was determined by 2D-TLC and GC-FID. The UV-Vis profile of compound 1 indicates a phenolic compound. Further analysis by using GC-MS shows one peak at a retention time (Rt) of 23.80 minutes, predicted as 4-4’-(1-methylethylidene)-bisphenol. The chemical constituent of the sub fraction 12.2.7 (fraction <strong>a</strong>) is identified as 4-4’-(1-methylethylidene)-bisphenol.</p>

scholarly journals PROLIFERATION INHIBITORY ACTIVITY OF THE ACTIVE FRACTION MARINE SPONGE Cinachyrella sp. AGAINST CELL LINE T47D

2015 ◽  
Vol 2 (1) ◽  
pp. 663
Author(s):  
Awik Puji Dyah Nurhayati ◽  
Rarastoeti Pratiwi ◽  
Subagus Wahyuono ◽  
Istriyati .
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Cell Line ◽  
Inhibitory Activity ◽  
Doubling Time ◽  
Preparative Thin Layer Chromatography ◽  
Marine Sponges ◽  
Active Fraction ◽  
Layer Chromatography

<p>Marine sponges Cinachyrella sp. (Family:Tetillidae) in Kukup beach, Kemadang Village, Tanjungsari District, Gunung Kidul, DIY were producing diversity secondary metabolites such as polyketides, alkaloids, peptide and terpene. The purpose of this study was investigated proliferation inhibitory activity of active fraction Cinachyrella sp. against cell line T47D. Sponges samples were collected manually from rocky substrate at depth 0.5 m. The sponges was minced and extracted with 95% ethanol. The ethanol extract was partitioned sequentially with ethyl acetate. The extract ethyl acetate was fractionation with 4 organic solvent, in increasing order of polarity with vacuum liquid chromatography column (VLC) method. Doubling time method was applied to analyse the inhibition proliferative cell line T47D. Resulted showed ethyl acetate extract of Cinachyrella sp. were 12 fractions and all tested fraction obtained by thin layer chromatography (TLC). Fractions that have the same value Rf grouped together to obtain 6 fractions. The fraction number 5 exhibited proliferation inhibitory activity to cell line T47D. The Rf value of active fraction number 5 were 0.125; 0.25 and 0.437. The active fraction 5 than isolation by preparative thin-layer chromatography (PTLC) was 5 isolate fractions preparative. The isolate fractions preparative number 5 exhibited proliferation inhibitory activity against cell line T47D. Fraction which determined by cerium sulfate and results was expressed terpene and alkaloid. <br /><strong></strong></p><p><strong>Keywords</strong>: Cinachyrella sp., Doubling time method, proliferation inhibitory activity.</p>

scholarly journals Isolasi Dan Identifikasi Terpenoid dari Fraksi n-Butanol Herba Lampasau (Diplazium esculentum Swartz)

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Maria Dewi Astuti ◽  
Evi Mintowati Kuntorini ◽  
Farah Eka Putri Wisuda
Keyword(s):  
Double Bond ◽  
Silica Gel ◽  
1H Nmr ◽  
Methanol Extract ◽  
Preparative Thin Layer Chromatography ◽  
Uv Spectra ◽  
Methyl Proton ◽  
Butanol Fraction ◽  
Uv Lamp ◽  
Layer Chromatography

Abstrak Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa kimia yang diisolasi dari fraksi n-butanol ekstrak metanol herba lampasau (Diplazium esculentum Swartz). Ekstrak metanol diperoleh secara maserasi dan difraksinasi berturut-turut denganpetroleum eter, etil asetat, dan n-butanol. Fraksi n­-butanol difraksinasidengan kromatografi kolom dengan fase diam silika gel dihasilkan fraksi A, B, C, dan D. Fraksi B dimurnikan dengan kromatografi lapis tipis preparatif pada silika geldihasilkan isolat B1. Isolat B1 berupa padatan tidak berwarna danberfluoresensi putih di bawah lampu UV 366 nm. Panjang gelombang maksimum pada spektra UV  isolat B1 adalah 225 nm dan 272.5 nm yang menunjukkan adanya ikatan rangkap tak terkonjugasi. Spektra IR isolat B1 menunjukkan adanya gugus C=C, –OH, C=O lakton, –CO, C–H ulur, dan C–H tekuk. Spektra 1H-NMR isolat B1 menunjukkan sinyal proton pada ikatan rangkap, proton –OH, proton pada –CH2 yang terikat atom oksigen, serta proton gugus metil –CH3. Berdasarkan data spektra UV, IR, dan 1H-NMR maka isolat B1 disarankan sebagai turunan senyawa triterpenoid hopan-lakton. Kata kunci : diplazium esculentum Swartz, fraksi n-butanol, triterpenoid hopan-lakton  Abstract The research  aims to identify chemical compounds isolated fromn-butanol fraction methanol extract of lampasau herbs (Diplazium esculentum Swartz). The methanol extract was obtained by maceration and fractioned by petroleum ether, ethyl acetate, andn-butanol. N-butanol fraction was fractionated using column chromatography on silica gel produced fractions A, B, C, and D. Fraction B was purified by preparative thin layer chromatography on silica gel produced isolate B1. Isolate B1was colorless solid and has white fluorescent under UV lamp 366 nm. The maximum wavelength on UV spectra of B1 are 225 nm and 272,5 nm indicates the unconjugated double bond. IR spectra of B1 showed the vibration of C=C, –OH, C=O lactone, –CO, C–H stretching and   C–H bending. Signals of 1H-NMR spectra of B1 showed the proton of double bond, –OH  proton, –CH2 proton bounded on oxygen atom, and –CH3 methyl proton. Based on data of spectra UV, IR, and 1H-NMR, isolate B1 suggested as a hopan triterpen derivative. Keywords : diplazium esculentum Swartz, n-butanol fraction, hopan-lactone triterpene

scholarly journals Potensi Insektisida Melur (Brucea javanica L. Merr) dalam Mengendalikan Hama Kubis Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) dan Plutella xylostella (L.) (Lepidoptera: Yponomeutidae)

2012 ◽  
Vol 12 (2) ◽  
pp. 109 ◽  
Author(s):  
Eka Candra Lina ◽  
Arneti Arneti ◽  
Djoko Prijono ◽  
Dadang Dadang
Keyword(s):  
Ethyl Acetate ◽  
Plutella Xylostella ◽  
Methanol Extract ◽  
Choice Test ◽  
Active Fraction ◽  
Brucea Javanica ◽  
Antifeedant Effect ◽  
Choice Tests ◽  
Layer Chromatography ◽  
Preparative Layer Chromatography

This study was conducted to evaluate the potency of Brucea javanica (melur) for controlling two species ofcrucifer pests, i.e. Crocidolomia pavonana and Plutella xylostella. Melur fruits, twigs, and leaves were extracteddirectly with methanol or sequentially with hexane, ethyl acetate, and methanol. The most active extract was thenfractionated by preparative layer chromatography using hexane, mixtures of ethyl acetate and methanol, andmethanol as eluents. The most active fraction was formulated as EC (emulsifiable concentrate) and WP (wettablepowder) formulations, and tested for their toxicity and antifeedant effect against C. pavonana and P. xylostellalarvae. The results showed that methanol extract of melur fruits was more active than that of twigs and leaves.Fractionation of methanol extract of melur fruits yielded an active fraction which was eluted with ethyl acetate-methanol 9:1. EC and WP formulations of melur fruits were active against C. pavonana larvae with LC50 of 0.39%and 0.21%, respectively. The same formulations were also active against P. xylostella larvae with LC50 of 0.31% and0.54%, respectively. In no-choice tests, the antifeedant effect of the EC formulation on C. pavonana larvae (feedinginhibition [FI]: 70.9%-97.5%) was higher than on P. xylostella larvae (FI: 52.2%-83.9%), but the antifeedant effect ofthe WP formulation on the two species was relatively the same. In a choice test, the EC formulation at LC 85completely inhibited feeding by C. pavonana larvae (FI: 100%).

scholarly journals Umbelliprenin from Ferula persica Roots Inhibits the Red Pigment Production in Serratia marcescens

2004 ◽  
Vol 59 (7-8) ◽  
pp. 506-508 ◽  
Author(s):  
Mehrdad Iranshahi ◽  
Ahmad R. Shahverdi ◽  
Roohollah Mirjani ◽  
Gholamreza Amin ◽  
Abbas Shafiee
Keyword(s):  
Antibacterial Activity ◽  
Thin Layer ◽  
Petroleum Ether ◽  
Serratia Marcescens ◽  
Preparative Thin Layer Chromatography ◽  
Chloroform Extract ◽  
Pigment Production ◽  
Red Pigment ◽  
Active Fraction ◽  
Layer Chromatography

AbstractThe chloroform extract of Ferula persica var. persica roots was found to inhibit red pigment production of Serratia marcescens. A bioguided fractionation study by preparative thin layer chromatography (PTLC) detected a fraction (Rf = 0.71, petroleum ether/EtOAc, 2:1 v/v), which was effective on depigmentation of Serratia marcescens. Using conventional spectroscopy methods, the active fraction was identified as umbelliprenin. Neither the chloroform extract nor the isolated umbelliprenin fraction showed any antibacterial activity against the test strain at a certain concentration. In contrast, they exhibited depigmentation zones on culture plates.

Anti-inflammatory effect of Mitragyna speciosa crude methanol extract on the guinea pig ileum

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
WM Shaik Mossadeq ◽  
K Syamimi ◽  
MP Azyyati ◽  
ZA Zakaria ◽  
AK Arifah ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Methanol Extract ◽  
Guinea Pig Ileum ◽  
Mitragyna Speciosa ◽  
Anti Inflammatory ◽  
Crude Methanol Extract ◽  
Inflammatory Effect
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