scholarly journals Wound Healing Effect of Essential Oil Extracted from Eugenia dysenterica DC (Myrtaceae) Leaves

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 2 ◽  
Author(s):  
Sandra Mazutti da Silva ◽  
Claudio Rezende Costa ◽  
Guilherme Martins Gelfuso ◽  
Eliete Silva Guerra ◽  
Yanna de Medeiros Nóbrega ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Migration ◽  
Essential Oil ◽  
Cell Line ◽  
The Body ◽  
No Production ◽  
Cam Assay ◽  
Skin Cell ◽  
Natural Oils ◽  
Eugenia Dysenterica

The use of natural oils in topical pharmaceutical preparations has usually presented safe agents for the improvement of human health. Based on research into the immense potential of wound management and healing, we aimed to validate the use of topical natural products by studying the ability of the essential oil of Eugenia dysenterica DC leaves (oEd) to stimulate in vitro skin cell migration. Skin cytotoxicity was evaluated using a fibroblast cell line (L929) by MTT assay. The oil chemical profile was investigated by GC-MS. Moreover, the inhibition of lipopolysaccharide (LPS) induced nitric oxide (NO) production in the macrophage cell line (RAW 264.7) tested. The Chick Chorioallantoic Membrane (CAM) assay was used to evaluate the angiogenic activity and irritating potential of the oil. The oEd induces skin cell migration in a scratch assay at a concentration of 542.2 µg/mL. α-humulene and β-caryophyllene, the major compounds of this oil, as determined by GC-MS, may partly explain the migration effect. The inhibition of nitric oxide by oEd and α-humulene suggested an anti-inflammatory effect. The CAM assay showed that treatment with oEd ≤ 292 µg/mL did not cause skin injury, and that it can promote angiogenesis in vivo. Hence, these results indicate the feasibility of the essential oil of Eugenia dysenterica DC leaves to developed dermatological products capable of helping the body to repair damaged tissue.

The Role of Nitric Oxide on the Proliferation of a Human Osteoblast Cell Line Stimulated With Hydroxyapatite

2008 ◽  
Vol 34 (4) ◽  
pp. 196-202 ◽  
Author(s):  
Wihaskoro Sosroseno ◽  
Erwan Sugiatno ◽  
Abdul Rani Samsudin ◽  
Mohd Fikri Ibrahim
Keyword(s):  
Nitric Oxide ◽  
Cell Proliferation ◽  
Cell Line ◽  
No Production ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Human Osteoblast Cell ◽  
Integrin Αv ◽  
Osteoblast Cell Line ◽  
Nitrite Production

Abstract The aim of the present study was to test the hypothesis that the proliferation of a human osteoblast cell line (HOS cells) stimulated with hydroxyapatite (HA) may be regulated by nitric oxide (NO). The cells were cultured on the surface of HA. Medium or cells alone were used as controls. L-arginine, D-arginine, 7-NI (an nNOS inhibitor), L-NIL (an iNOS inhibitor), L-NIO (an eNOS inhibitor) or carboxy PTIO, a NO scavenger, was added in the HA-exposed cell cultures. The cells were also precoated with anti-human integrin αV antibody. The levels of nitrite were determined spectrophotometrically. Cell proliferation was assessed by colorimetric assay. The results showed increased nitrite production and cell proliferation by HA-stimulated HOS cells up to day 3 of cultures. Anti-integrin αV antibody, L-NIO, or carboxy PTIO suppressed, but L-arginine enhanced, nitrite production and cell proliferation of HA-stimulated HOS cells. The results of the present study suggest, therefore, that interaction between HA and HOS cell surface integrin αV molecule may activate eNOS to catalyze NO production which, in turn, may regulate the cell proliferation in an autocrine fashion.

scholarly journals Effect of Cadmium Exposure in Polymorphisms Gene NOS3, Blood Cadmium Level, Nitric Oxide Level, Blood Pressure and Antioxidant Enzymes

2018 ◽  
Vol 73 ◽  
pp. 06006
Author(s):  
Hernayanti ◽  
Santoso Slamet ◽  
Lestari Sri
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Antioxidant Enzyme ◽  
Human Life ◽  
The Body ◽  
No Production ◽  
Textile Materials ◽  
Length Polymorphisms ◽  
Exposed Individual ◽  
Result Show

Cadmium is one of a heavy metal which widely used in human life, especially in the electroplating industry and a mixture of textile materials. Cadmium that enters the body binds to the metallothioneins protein. It can increase the formation of free radical compounds, there by inhibiting enzyme activity such as nitric oxide synthase3. This gene regulates the expression of endothelial nitric oxide synthase which produce a nitric oxide. Nitric oxide role in regulated blood pressure as vasodilator with Angiotensin II as vasoconstriction. The susceptibility to Cd exposure will elevate if the polymorphisms of gene is found in population. The aim of this research was to know effect of cadmium to gene NOS3 polymorphisms on NO, systolic and diastolic blood pressure and antioxidant enzyme in Cd-exposed individual. The genotype individual were detected by Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms (PCR-RLFP) with MBo1 restriction enzyme. Parameter recorded were blood Cd , NO level, SOD, systolic and diastolic. Data were analyzed by independent t-test. These result showed that 20% of 40 individual of cases subject were detected as polymorphisms individual of NOS3gene, with GA genotype. Their fragment DNA located on 206 bp, 119 bp and 87 bp, but non polymorphisms of NO gene is only located on 206 bp. The result show cadmium could influence polymorphisms NOS3gene and decrease NO production followed by increasing of blood pressure both systolic and diastolic. Cadmium also decrease antioxidant enzyme SOD and GPx level.

scholarly journals Control of IntracellularFrancisella tularensisby Different Cell Types and the Role of Nitric Oxide

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Sarah L. Newstead ◽  
Amanda J. Gates ◽  
M. Gillian Hartley ◽  
Caroline A. Rowland ◽  
E. Diane Williamson ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Line ◽  
Rational Design ◽  
Cell Types ◽  
No Production ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Schu S4 ◽  
Intracellular Infections

Reactive nitrogen is critical for the clearance ofFrancisella tularensisinfections. Here we assess the role of nitric oxide in control of intracellular infections in two murine macrophage cell lines of different provenance: the alveolar macrophage cell line, MH-S, and the widely used peritoneal macrophage cell line, J774A.1. Cells were infected with the highly virulent Schu S4 strain or with the avirulent live vaccine strain (LVS) with and without stimuli. Compared to MH-S cells, J774A.1 cells were unresponsive to stimulation and were able to control the intracellular replication of LVS bacteria, but not of Schu S4. In MH-S cells, Schu S4 demonstrated control over cellular NO production. Despite this, MH-S cells stimulated with LPS or LPS and IFN-γwere able to control intracellular Schu S4 numbers. However, only stimulation with LPS induced significant cellular NO production. Combined stimulation with LPS and IFN-γproduced a significant reduction in intracellular bacteria that occurred whether high levels of NO were produced or not, indicating that NO secretion is not the only defensive cellular mechanism operating in virulentFrancisellainfections. Understanding howF. tularensisinteracts with host macrophages will help in the rational design of new and effective therapies.

Antineuroinflammatory constituents from the root extract of Paris verticillata

2011 ◽  
Vol 89 (4) ◽  
pp. 441-445 ◽  
Author(s):  
Ki Hyun Kim ◽  
Kyu Ha Lee ◽  
Ho Kyung Kim ◽  
Eunjung Moon ◽  
Sung-Hoon Kim ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fatty Acid ◽  
Cell Line ◽  
Spectroscopic Data ◽  
2D Nmr ◽  
No Production ◽  
Root Extract ◽  
Meoh Extract ◽  
Microglia Cell ◽  
Chemical Evidence

Two new cyclopropanoic fatty acid glycosides, named parisveroside A (1) and parisveroside B (2), were isolated from a MeOH extract of the roots of Paris verticillata (Liliaceae) together with three other known compounds, salicin (3), 3-(β-d-glucopyranosyloxymethyl)-2-(4-hydroxy-3-methoxyphenyl)-5-(3-hydroxypropyl)-7-methoxy-(2R,3S)-dihydrobenzofuran (4), and allantoin (5). Their structures were elucidated on the basis of spectroscopic data, including 1D and 2D NMR, HR-FAB-MS, and chemical evidence. To investigate the antineuroinflammatory effects of the isolated compounds (1–5), nitric oxide (NO) production was evaluated in the lipopolysaccharide-activated microglia cell line, BV-2. Compounds 2 and 4 significantly inhibited NO production with IC50 values of 74.8 and 60.5 µmol/L, respectively.

scholarly journals Four New Iridoid Metabolites Have Been Isolated from the Stems of Neonauclea reticulata (Havil.) Merr. with Anti-Inflammatory Activities on LPS-Induced RAW264.7 Cells

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4271
Author(s):  
Fang-Pin Chang ◽  
Shyh-Shyun Huang ◽  
Tzong-Huei Lee ◽  
Chi-I Chang ◽  
Tzong-Fu Kuo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Detailed Analysis ◽  
Cell Line ◽  
Spectroscopic Data ◽  
Protocatechuic Acid ◽  
Control Group ◽  
No Production ◽  
Raw264.7 Macrophages ◽  
Ic50 Values ◽  
Inhibitory Activities

One new iridoid, namely neonanin C (1) one monocyclic iridoid ring-opened derivative namely neonanin D (2), two new bis-iridoid derivatives namely reticunin A (3) and reticunin B (4) with sixteen known compounds (5–20) were isolated from the stems of Neonauclea reticulata (Havil.) Merr. These new structures were determined by the detailed analysis of spectroscopic data and comparison with the data of known analogues. Compounds 1–20 were evaluated for inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages cell line. The results showed that all compounds exhibited no obvious cytotoxicity compared to the control group and five compounds including isoboonein (7), syringaresinol (10), (+)-medioresinol (12), protocatechuic acid (14) and trans-caffeic acid (15) exhibited inhibitory activities with IC50 values at 86.27 ± 3.45; 9.18 ± 1.90; 76.18 ± 2.42; 72.91 ± 4.97 and 95.16 ± 1.20 µg/mL, respectively.

Enhanced Expression of Inducible Nitric Oxide Synthase by Juzen-Taiho-To in LPS-Activated RAW264.7 Cells, a Murine Macrophage Cell Line

2000 ◽  
Vol 28 (02) ◽  
pp. 217-226 ◽  
Author(s):  
Hiroshi Kawamata ◽  
Hiroshi Ochiai ◽  
Naoki Mantani ◽  
Katsutoshi Terasawa
Keyword(s):  
Nitric Oxide ◽  
Mrna Expression ◽  
Cell Line ◽  
Raw264.7 Cells ◽  
Inos Mrna ◽  
No Production ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line

We have investigated the effect of Juzen-taiho-to (TJ-48) on inducible NO synthase (iNOS) expression and nitric oxide (NO) production in RAW264.7 cells, a murine macrophage cell line. TJ-48-lipopolysaccharide (LPS) combination induced iNOS mRNA expression earlier, stronger and remained longer that paralleled but with a higher NO production compared to LPS stimulation. TJ-48 itself showed no inducible effect either no NO production or iNOS mRNA expression. This phenomenon could be considered to contribute, at least in part, to the beneficial effects of TJ-48 through the iNOS-mediated activation of biodefense mechanism.

scholarly journals Neuropeptide Y Reduces Nasal Epithelial T2R Bitter Taste Receptor–Stimulated Nitric Oxide Production

Nutrients ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 3392
Author(s):  
Ryan M. Carey ◽  
Nithin D. Adappa ◽  
James N. Palmer ◽  
Robert J. Lee
Keyword(s):  
Nitric Oxide ◽  
Neuropeptide Y ◽  
Bitter Taste ◽  
Beat Frequency ◽  
Taste Receptor ◽  
Ciliary Beat Frequency ◽  
Fluid Secretion ◽  
The Body ◽  
No Production ◽  
Neuropeptide Tyrosine

Bitter taste receptors (T2Rs) are G-protein-coupled receptors (GPCRs) expressed on the tongue but also in various locations throughout the body, including on motile cilia within the upper and lower airways. Within the nasal airway, T2Rs detect secreted bacterial ligands and initiate bactericidal nitric oxide (NO) responses, which also increase ciliary beat frequency (CBF) and mucociliary clearance of pathogens. Various neuropeptides, including neuropeptide tyrosine (neuropeptide Y or NPY), control physiological processes in the airway including cytokine release, fluid secretion, and ciliary beating. NPY levels and/or density of NPYergic neurons may be increased in some sinonasal diseases. We hypothesized that NPY modulates cilia-localized T2R responses in nasal epithelia. Using primary sinonasal epithelial cells cultured at air–liquid interface (ALI), we demonstrate that NPY reduces CBF through NPY2R activation of protein kinase C (PKC) and attenuates responses to T2R14 agonist apigenin. We find that NPY does not alter T2R-induced calcium elevation but does reduce T2R-stimulated NO production via a PKC-dependent process. This study extends our understanding of how T2R responses are modulated within the inflammatory environment of sinonasal diseases, which may improve our ability to effectively treat these disorders.

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