scholarly journals Phenolic Composition, Antioxidant Properties, and Inhibition toward Digestive Enzymes with Molecular Docking Analysis of Different Fractions from Prinsepia utilis Royle Fruits

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3373 ◽  
Author(s):  
Xuan Zhang ◽  
Yijia Jia ◽  
Yanli Ma ◽  
Guiguang Cheng ◽  
Shengbao Cai
Keyword(s):  
Molecular Docking ◽  
Phenolic Compounds ◽  
Digestive Enzymes ◽  
Digestive Enzyme ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Docking Analysis ◽  
Molecular Docking Analysis

The present study investigated the phenolic profiles and antioxidant properties of different fractions from Prinsepia utilis Royle fruits using molecular docking analysis to delineate their inhibition toward digestive enzymes. A total of 20 phenolics was identified and quantified. Rutin, quercetin-3-O-glucoside, and isorhamnetin-3-O-rutinoside were the major phenolic compounds in the total phenolic fraction and flavonoid-rich fraction. The anthocyanin-rich fraction mainly contained cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside. All of the fractions exhibited strong radical scavenging activities and good inhibition on cellular reactive oxygen species (ROS) generation in H2O2-induced HepG2 cells, as evaluated by DPPH and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays. Moreover, the powerful inhibitory effects of those fractions against pancreatic lipase and α-glucosidase were observed. The major phenolic compounds that were found in the three fractions also showed good digestive enzyme inhibitory activities in a dose-dependent manner. Molecular docking analysis revealed the underlying inhibition mechanisms of those phenolic standards against digestive enzymes, and the theoretical analysis data were consistent with the experimental results.

scholarly journals HPLC-ESI-qTOF-MS/MS Characterization, Antioxidant Activities and Inhibitory Ability of Digestive Enzymes with Molecular Docking Analysis of Various Parts of Raspberry (Rubus ideaus L.)

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 274 ◽  
Author(s):  
Lingfeng Wu ◽  
Yufeng Liu ◽  
Yin Qin ◽  
Lu Wang ◽  
Zhenqiang Wu
Keyword(s):  
Molecular Docking ◽  
Phenolic Compounds ◽  
Digestive Enzymes ◽  
Antioxidant Activities ◽  
Rubus Idaeus ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Inhibitory Effects ◽  
Docking Analysis ◽  
Molecular Docking Analysis

The anti-oxidative phenolic compounds in plant extracts possess multiple pharmacological functions. However, the phenolic characterization and in vitro bio-activities in various parts of raspberry (Rubus idaeus L.) have not been investigated systematically. In the present study, the phenolic profiles of leaves (LE), fruit pulp (FPE), and seed extracts (SE) in raspberry were analyzed by HR-HPLC-ESI-qTOF-MS/MS method, and their antioxidant activities and digestive enzymes inhibitory abilities were also investigated. The molecular docking analysis was used to delineate their inhibition mechanisms toward type II diabetes related digestive enzymes. Regardless of LE, FPE, or SE, 50% methanol was the best solvent for extracting high contents of phenolic compounds, followed by 50% ethanol and 100% methanol. The LE of raspberry displayed the highest total phenolic content (TPC) and total flavonoid content (TFC). A total of nineteen phenolic compounds were identified. The quantitative results showed that gallic acid, ellagic acid, and procyanidin C3 were the major constituents in the three extracts. The various parts extracts of raspberry all exhibited the strong antioxidant activities, especially for LE. Moreover, the powerful inhibitory effects of the three extracts against digestive enzymes (α-glucosidase and α-amylase) were observed. The major phenolic compounds of the three extracts also showed good inhibitory activities of digestive enzyme in a dose-dependent manner. The underlying inhibitory mechanisms of the main phenolic compounds against digestive enzymes were clarified by molecular docking analysis. The present study demonstrated that the various parts of raspberry had strong antioxidant activities and inhibitory effects on digestive enzymes, and can potentially prevent oxidative damage or diabetes-related problems.

Strong antihemolytic and antioxidant properties of aqueous extract from Algerian Hammada elegans (Bge.) Botsch (Chenopodiaceae)

2021 ◽  
Vol 17 ◽  
Author(s):  
Brahim Asseli ◽  
Reguia Mahfoudi ◽  
Amar Djeridane ◽  
Mohamed Yousfi
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Condensed Tannin ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Vitro Assay

Background: Research on medicinal plant antioxidants has emerged as a potential therapeutic to prevent free radical generated damage in the human body. Hammada elegans Botsch (popularly known as “Ajram”) is a xerophytic plant widely found in Laghouat region, but there are only a few reports about the biological or chemical properties of these species. Hence, the aim of this study is to investigate the antioxidant and the antihemolytic activities of hexanic, acetonic, methanolic and aqueous extracts of aerial parts of Algerian Hammada elegans Botsch by employing different in vitro assay systems. Methods: The total phenolic content, the flavonoid content and the condensed tannin amount were analyzed using Folin-Ciocalteu, aluminum chloride and vanillin assays, respectively. The in vitro antioxidant capacity of extracts was assessed by CUPRAC, iron chelating, ABTS•+and antihemolytic assays, and was expressed as EC50 values. Results: Among the analyzed extracts, the aqueous extract had the highest phenolic, flavonoid and tannin contents. Also, this extract displayed the highest antioxidant capacities compared to the other extracts and standards. Its EC50 value for ABTS radical-scavenging activity was 0.265 ± 0.003 mg/L. Moreover, this extract showed high iron (II) chelating ability (EC50 = 0.958 ± 0.001 mg/L), and good antioxidant activity in the cupric ion reducing activity (CUPRAC) in a concentration dependent manner (EC50 were 0.709 ± 0.002 mg/L). Additionally, this extract had the best antihemolytic activity against AAPH-induced hemolysis (EC50=0.090 ± 0.004 mg/L). Conclusion: Our study revealed that the aqueous extract of Hammada elegans Botsch, is a potential source of antioxidants which possess a high protective effect of membrane against free radical.

scholarly journals Effect of different extraction solvents on antioxidant properties of water lily (Nymphaeaceae antares) flower petal and stamen

Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 359-368
Author(s):  
Z. Mohd Zin ◽  
A.Z.M. Sanuri ◽  
N. Bashah ◽  
K. Ibrahim ◽  
H. Yahya ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Water Lily ◽  
Antioxidative Properties ◽  
Extraction Solvents

Water lily (Nymphaeaceae antares) contains a vast array of phenolic compounds with important natural antioxidative properties that could inhibit the adverse effects of the reactive oxygen species produced in living things. These substances are complex in composition which requires a suitable extraction medium to exploit the phenolic compounds. This study was designed to determine the antioxidative properties in petal and stamen of water lily extracted using different extraction solvents namely ethyl acetate and 60% methanol. Antioxidant properties of the samples were determined using 2,2-diphenyl -1-picrylhydrazyl (DPPH) radical scavenging activity, ferric thiocyanate (FTC) method, thiobarbituric acid (TBA) test, total phenolic content (TPC), total flavonoid content (TFC) and total tannin content (TTC) along with individual flavonoids content using High Performance Liquid Chromatography (HPLC). The yield of extract was found to be the highest in petals extracted with 60% methanol (44.55±1.63%) while stamen extracted with ethyl acetate yielded the lowest (24.50±1.09%). In the DPPH inhibition method, petal extracted with ethyl acetate obtained the highest scavenging activity (89.15±3.62%). FTC analysis revealed that ethyl acetate extract of petal experienced the highest percentage inhibition (76.70±6.48%) while in TBA analysis ethyl acetate extract of stamen exhibited the highest percentage inhibition (70.78±1.39%). Flavonoids such as quercetin, kaempferol, rutin, gallic acid, catechin, epigallocatechin, p-coumaric acid and myricetin were found to be present in ethyl acetate and 60% methanol extracts of water lilies stamen and petal. The information from this study indicates that water lily petals and stamens may be integrated as a good source of nutrients in the food industry, the pharmaceutical industry. If the advantages of water lily are not used and integrated into everyday life for health benefits, it will be a massive waste of natural resources.

scholarly journals Secondary Metabolites Profiling of Acinetobacter baumannii Associated with Chili (Capsicum annuum L.) Leaves and Concentration Dependent Antioxidant and Prooxidant Properties

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Tahmina Monowar ◽  
Md. Sayedur Rahman ◽  
Subhash J. Bhore ◽  
Gunasunderi Raju ◽  
Kathiresan V. Sathasivam
Keyword(s):  
Phenolic Compounds ◽  
Secondary Metabolites ◽  
Metal Ions ◽  
Acinetobacter Baumannii ◽  
Endophytic Bacteria ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Total Antioxidant

Secondary bioactive compounds of endophytes are inevitable biomolecules of therapeutical importance. In the present study, secondary metabolites profiling of an endophytic bacterial strain, Acinetobacter baumannii, were explored using GC-MS study. Presence of antioxidant substances and antioxidant properties in chloroform (CHL), diethyl ether (DEE), and ethyl acetate (EA) crude extracts of the endophytic bacteria were studied. Total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (TAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and ferrous ion chelating assay were evaluated. A total of 74 compounds were identified from the GC-MS analysis of the EA extract representing mostly alkane compounds followed by phenols, carboxylic acids, aromatic heterocyclic compounds, ketones, aromatic esters, aromatic benzenes, and alkenes. Among the two phenolic compounds, namely, phenol, 2,4-bis(1,1-dimethylethyl)- and phenol, 3,5-bis(1,1-dimethylethyl)-, the former was found in abundance (11.56%) while the latter was found in smaller quantity (0.14%). Moreover, the endophytic bacteria was found to possess a number of metal ions including Fe(II) and Cu(II) as 1307.13 ± 2.35 ppb and 42.38 ± 0.352 ppb, respectively. The extracts exhibited concentration dependent antioxidant and prooxidant properties at high and low concentrations, respectively. The presence of phenolic compounds and metal ions was believed to play an important role in the antioxidant and prooxidant potentials of the extracts. Further studies are suggested for exploring the untapped resource of endophytic bacteria for the development of novel therapeutic agents.

scholarly journals Assessment of Antioxidant Properties in Fruits ofMyrica esculenta: A Popular Wild Edible Species in Indian Himalayan Region

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Sandeep Rawat ◽  
Arun Jugran ◽  
Lalit Giri ◽  
Indra D. Bhatt ◽  
Ranbeer S. Rawal
Keyword(s):  
Phenolic Compounds ◽  
Gallic Acid ◽  
Significant Variation ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Himalayan Region ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Indian Himalayan Region ◽  
Edible Species

Crude extract ofMyrica esculentafruits, a wild edible species of Indian Himalayan Region, was evaluated for phenolic compounds and antioxidant properties. Results revealed significant variation in total phenolic and flavonoid contents across populations. Among populations, total phenolic content varied between 1.78 and 2.51 mg gallic acid equivalent/g fresh weight (fw) of fruits and total flavonoids ranged between 1.31 and 1.59 mg quercetin equivalent/g fw. Antioxidant activity determined by 2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging, 1,1-diphenyl-2-picrylhydrazyl radical scavenging and ferric reducing antioxidant power (FRAP) exhibited considerable antioxidant potential and showed significant positive correlation with total phenolic and total flavonoids content. High performance liquid chromatography analysis revealed significant variation (P<  .01) in phenolic compounds (i.e., gallic acid, catechin, hydroxybenzioc acid andρ-coumaric acid) across populations. This study provides evidences to establish that consumption ofM. esculentafruits while providing relished taste would also help in reduction of free radicals. Therefore, this wild edible species deserves promotion in the region through horticulture and forestry interventions.

scholarly journals Phenolic Compounds of Soybean Seeds from Two European Countries and Their Antioxidant Properties

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2075
Author(s):  
Angelika Król-Grzymała ◽  
Ryszard Amarowicz
Keyword(s):  
Phenolic Compounds ◽  
Defense Mechanism ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Cold Climate ◽  
Total Phenolic ◽  
Soybean Seeds ◽  
Phenolic Contents ◽  
Soybean Cultivars

There is only a small acreage of planted soybeans in northern Europe, as the global production of this crop is mainly dictated by the warmer temperatures needed for bountiful yields. The defense response of soybean plants to a cold climate entails the secretion of specific compounds which help mitigate oxidative stress, i.e., antioxidants, including phenolic compounds. The objective of this study was to examine differences in the concentrations of phenolic compounds, their antioxidant properties, and the concentration of key isoflavones (namely genistein, daidzein, malonyl daidzein, malonyl genistein, and daidzin) in the seeds of six soybean cultivars from two different regions of Europe, namely Poland and France. The total phenolic contents, isoflavone levels, and in vitro antioxidant capacities of soybean seeds from most of the investigated cultivars of northeast Europe were found to be greater than those from southwest Europe. The phenolic compounds of seed extracts are primarily responsible for the free-radical scavenging of soybeans. Factors regulating the production of phenolic compounds in the seeds have not been thoroughly elucidated. Hence, the results presented in this paper can be useful in the selection of soybean cultivars with higher levels of seed phenolics, because of their beneficial impact on human health and on the soybean’s defense mechanism against plant stresses.

scholarly journals Determination of Antioxidant Properties of 26 Chilean Honeys and a Mathematical Association Study with their Volatile Profile

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Elizabeth Sánchez ◽  
Marisa Piovano ◽  
Erika Valdés ◽  
Manuel E. Young ◽  
Cristian A. Acevedo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Persea Americana ◽  
Total Phenolic ◽  
Volatile Profile ◽  
Total Phenolic Compounds ◽  
Latent Structures

Radical scavenging activity (RSA), antioxidant content (TEAC), total phenolic compounds content (TPCC) and volatile profile (VOCs) were measured in 26 honeys obtained from the Valparaíso Region (Chile). Persea americana honey was the most interesting sample according to these evaluated parameters. A Projection to Latent Structures (PLS) based algorithm was used to model the possible relationship between antioxidant activity, total phenolic compounds content and volatile profile. Concerning the volatile profile, only nine volatile compounds, of a total of fifty, showed dependence on antioxidant activity and total phenolic compounds content.

scholarly journals Effect of boiling and roasting on physicochemical and antioxidant properties of dark red kidney bean (Phaseolus vulgaris)

Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 438-445
Author(s):  
M. Roy ◽  
M.Z.H. Imran ◽  
M. Alam ◽  
M. Rahman
Keyword(s):  
Phenolic Compounds ◽  
Phaseolus Vulgaris ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Kidney Bean ◽  
Total Phenolic ◽  
Quality Health ◽  
Gross Energy ◽  
Red Kidney Bean

The effects of two processing methods (boiling and roasting) on physicochemical qualities of dark red kidney bean (Phaseolus vulgaris) were investigated. Changes in total polyphenol content (TPC) and antioxidant activity due to processing were also analyzed by using Folin–Ciocaltue method and 1,1 diphenyl-2-picrylhydrazyl (DPPH) assay, respectively. Analysis of physicochemical properties exhibited substantial variations (p<0.05) in the levels of moisture, ash, protein, fat, carbohydrate, gross energy and pH of raw and processed beans. Meanwhile, there was no considerable difference (p<0.05) in the fibre content of fresh and processed beans. Investigation of total phenolic content showed the significant (p<0.05) reduction of phenolic compounds in boiled (5.53±0.74 mg GAE/ g) and roasted (8.15±1.60 mg GAE/g) beans than raw samples (12.55±1.42 mg GAE/g). DPPH radical scavenging activity was also decreased significantly (p<0.05) in boiled and roasted samples with IC50 value of 0.0608±0.0765 mg/mL and 0.0523±0.0026 mg/mL, respectively. In the case of household cooking, keeping boiling water would be advised for the preservation of minerals, vitamins and phenolic compounds. The roasting process may be used to develop high-quality health-promoting dark red kidney bean products.

scholarly journals Potential antioxidant effects of Narcissus tazetta phenolic compounds against cadmium chloride induced hepatotoxicity in Swiss albino mice

2021 ◽  
Author(s):  
Saoussen Ben-Abdallah ◽  
Mediha Sefi ◽  
Nejla Soudani ◽  
Amel Hamdi ◽  
Safa Bejaoui ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Cadmium Chloride ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Protective Effects ◽  
Gamma Glutamyl Transpeptidase ◽  
Narcissus Tazetta ◽  
Protein Thiols

Abstract Narcissus tazetta (Amaryllidaceae) is a medicinal plant, widely used for cut flowers and potted ornamental plant in Tunisia flora. The current study evaluated the phenolic composition and antioxidant properties of its flower extracts and investigated its potential protective activity against cadmium chloride (CdCl2)-induced hepatotoxicity in mice. Adult Swiss mice were exposed either to CdCl2 (0.16 mg/kg bw) or to Narcissus tazetta extract (100 or mg/kg bw) or to their combination. Cadmium chloride and Narcissus tazetta flower extracts were given by intraperitoneal injection and gavage respectively for eight consecutive days. Results indicated that ethanolic flower extracts contained high levels of total phenolic and flavonoid contents along with a strong total antioxidant and 2,2-Diphenyl-1-picrylhydrazyl free radical scavenging activities. High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) analysis identified eight phenolic compounds, including rutin, Kaempferol glycosides and chlorogenic acids. The extract also exhibited marked hepato-protective effects against CdCl2 toxicity by reducing hepatic levels of malondialdehyde, advanced oxidation protein products, hydrogen peroxide, metallothioneins and DNA degradation. Additionally, co-administration of Narcissus tazetta flower extracts lowered the plasma activities of transaminases, gamma glutamyl transpeptidase and lactate dehydrogenase, and increased hepatic levels of reduced glutathione, non-protein thiols, vitamin C, and catalase activity. The hepatoprotective effects of the extract were demonstrated by histopathological improvement of liver disorders. The current study provided ethnopharmacological application of Narcissus tazetta flower extracts against CdCl2 induced oxidative stress, suggesting its chemoprevention role of its phenolic compounds as a natural antioxidant.

scholarly journals Evaluation of Phenolic Compounds in Cistus creticus Bee Pollen from Greece. Antioxidant and Antimicrobial Properties

2017 ◽  
Vol 12 (11) ◽  
pp. 1934578X1701201 ◽  
Author(s):  
Emmanuel Atsalakis ◽  
Ioanna Chinou ◽  
Maria Makropoulou ◽  
Sofia Karabournioti ◽  
Konstantia Graikou
Keyword(s):  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Total Phenolic ◽  
Bee Pollen ◽  
Butanol Extract ◽  
Cistus Creticus

This study was carried out to evaluate the antioxidant properties of Greek endemic Cistus creticus L. (rock rose) bee pollen and define its phenolic compounds. In the framework of our scientific studies on Greek bee keeping products, we report herein our research on three Greek bee pollen samples from Cistus. Their pollinic spectra were obtained by Louveaux's quantitative microscopical analysis and it showed that one of them had Cistus sp. (Cistaceae) as abundant pollen (together with low percentage of Brassica sp. (Cruciferae). Throughout the chemical analysis of the extracts, several secondary metabolites of flavonoid structure have been identified as major components. Specifically, quercetin-7-rhamnoside (1), quercetin-3-neohesperidoside (2), kaempferol-3-neohesperidoside (3), myricetin-3-neohesperidoside (4), kaempferol-3-glucoside (5) and quercetin-3-glucoside (6) have been isolated and their structures were elucidated on the basis of spectral evidence. Moreover, the total phenolic and flavonoid content was estimated and the free radical scavenging activity was determined by DPPH and ABTS assays. The antimicrobial activity of the extracts was tested against six Gram-positive and -negative bacteria and three pathogenic fungi, and the butanol extract showed a very interesting broad antimicrobial profile (MIC 1.98•10−3 - 2.98•10−3 mg/ml) against all the assayed microorganisms.

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