scholarly journals Expression of Bioactive Lunasin Peptide in Transgenic Rice Grains for the Application in Functional Food

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2373 ◽  
Author(s):  
Guixing Ren ◽  
Yuqiong Hao ◽  
Yingying Zhu ◽  
Zhenxing Shi ◽  
Gang Zhao
Keyword(s):  
Antioxidant Activity ◽  
Transgenic Rice ◽  
Functional Food ◽  
Radical Scavenging Activity ◽  
Expression System ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Anti Inflammatory

Lunasin, a bioactive peptide initially isolated from soybean, has anticancer, anti-inflammatory, and antioxidant activity. Due its great application value, lunasin seems to be a candidate gene in improving the nutritional value of crops. In this study, lunasin was inserted into the rice genome to evaluate whether it was feasible to express lunasin using the rice expression system and improve the bioactivity of protein in rice for our needs. We generatedlunasin-overexpressing rice lines, and chose three independent transgenic rice lines for further study. The lunasin content in trans-lunasin rice detected by UPLC-MS/MS was 1.01 × 10−3 g·kg−1 dry rice flour with grease removal in the lunasin extracts. The antioxidant efficacy of LET (lunasin-enriched fraction from trans-lunasin rice) and PEW (peptide-enriched fraction from wild type rice) was compared. Due to the presence of lunasin, LET showed higher (p < 0.05) antioxidant activity than PEW. LET exhibited high DPPH radical scavenging activity (IC50 value, 8 g·L−1), strong ABTS+ radical scavenging activity (IC50 value, 1.18 g·L−1), and great oxygen radical scavenging activity (170 μmol·L−1 Trolox equivalents when the concentration reached 4 g·L−1). Moreover, LET presented significantly higher (p < 0.05) anti-inflammatory activity on macrophage cells, and the NO production and the release of pro-inflammatory cytokines (IL-6, MCP1, and TNF-α) were significantly inhibited by LET. However, because of the low purity, LET showed weaker antioxidant and anti-inflammatory activity when compared to the Lunasin standard. These results suggested that it is feasible to use the rice expression system to express the exogenous lunasin in rice, and lunasin-overexpressing rice seems to be a candidate resource for application in functional food. Rice rich in lunasin is beneficial for human health, and could be used as a functional food in the diets of cancer and obese patients in the future.

scholarly journals Antioxidant and Potentially Anti-Inflammatory Activity of Anthocyanin Fractions from Pomace Obtained from Enzymatically Treated Raspberries

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 299 ◽  
Author(s):  
Urszula Szymanowska ◽  
Barbara Baraniak
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Solid Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Phase Extraction ◽  
Scavenging Activity ◽  
Hplc Technique ◽  
Anti Inflammatory

Raspberry pomace was obtained from raspberries subjected to enzymatic maceration using three commercial pectinolytic preparations (Pectinex Ultra SP-L, Pectinex Yield Mash, and Ultrazym AFP-L). Phenolic compounds were extracted and anthocyanin fractions were isolated using the SPE solid phase extraction technique. In the separated anthocyanin fractions, the content of individual compounds was determined by the HPLC technique and the antioxidant activity was assessed with four complementary methods (DPPH and ABTS radical scavenging activity, chelating Fe(II) power, and ferric reducing power). Potential anti-inflammatory properties were also identified as the ability to inhibit the activity of lipoxygenase and cyclooxygenase 2. For these enzymes, the type of inhibition was determined based on the Lineweaver–Burke plot.

scholarly journals In vitro assessment of the protection from oxidative stress by various fractions of Artemisia incisa Pamp.

2011 ◽  
Vol 76 (10) ◽  
pp. 1379-1386
Author(s):  
A Aziz-Ur-Rehman ◽  
Sehrish Gulzar ◽  
Muhammad Abbasi ◽  
Tayyaba Shahzadi ◽  
Tauheeda Riaz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aqueous Fraction ◽  
Antioxidant Power ◽  
Ic50 Value

The methanolic extract of Artemisia incisa Pamp. was dissolved in distilled water and successively partitioned with n-hexane, chloroform, ethyl acetate and n-butanol. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods, i.e., the scavenging activity of the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), the total antioxidant activity, the ferric reducing antioxidant power (FRAP) assay and the ferric thiocyanate assay. In addition, the total phenolics was determined. The obtained results revealed that among the studied fractions the ethyl acetate soluble fraction showed the most potent DPPH-radical scavenging activity with an IC50 value of 5.3 ? 0.71 ?g mL-1, which is even more effective than the standard antioxidant butylated hydroxytoluene (BHT) (IC50 value 0f 12.1? 0.92 ?g mL-1). The ethyl acetate fraction also showed the highest FRAP value (3677.13 ? 27.1 ?g TE mL-1), inhibition of lipid peroxidation (60.93 ? 0.84 % at 500 ?g mL-1) and total phenolic content (95.5 ? 0.05 ?g GAE g-1) as compared to other fractions. However, the remaining aqueous fraction was found to posses the highest antioxidant activity of all the fractions.

scholarly journals Antioxidant and Anti-Inflammatory Activity Determination of One Hundred Kinds of Pure Chemical Compounds Using Offline and Online Screening HPLC Assay

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Kwang Jin Lee ◽  
You Chang Oh ◽  
Won Kyung Cho ◽  
Jin Yeul Ma
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chemical Compounds ◽  
Cell Counting ◽  
Raw 264.7 Cells ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Abts Assay ◽  
Anti Inflammatory

This study investigated the antioxidant activity of one hundred kinds of pure chemical compounds found within a number of natural substances and oriental medicinal herbs (OMH). Three different methods were used to evaluate the antioxidant activity of DPPH radical-scavenging activity, ABTS radical-scavenging activity, and online screening HPLC-ABTS assays. The results indicated that 17 compounds exhibited better inhibitory activity against ABTS radical than DPPH radical. The IC50rate of a more practical substance is determined, and the ABTS assay IC50values of gallic acid hydrate, (+)-catechin hydrate, caffeic acid, rutin hydrate, hyperoside, quercetin, and kaempferol compounds were 1.03 ± 0.25, 3.12 ± 0.51, 1.59 ± 0.06, 4.68 ± 1.24, 3.54 ± 0.39, 1.89 ± 0.33, and 3.70 ± 0.15 μg/mL, respectively. The ABTS assay is more sensitive to identifying the antioxidant activity since it has faster reaction kinetics and a heightened response to antioxidants. In addition, there was a very small margin of error between the results of the offline-ABTS assay and those of the online screening HPLC-ABTS assay. We also evaluated the effects of 17 compounds on the NO secretion in LPS-stimulated RAW 264.7 cells and also investigated the cytotoxicity of 17 compounds using a cell counting kit (CCK) in order to determine the optimal concentration that would provide an effective anti-inflammatory action with minimum toxicity. These results will be compiled into a database, and this method can be a powerful preselection tool for compounds intended to be studied for their potential bioactivity and antioxidant activity related to their radical-scavenging capacity.

scholarly journals PHENOLIC COMPOUND FROM THE STEM BARK OF MANGGIS HUTAN (Garcinia bancana Miq.) AND THEIR ANTIOXIDANT ACTIVITY

2010 ◽  
Vol 9 (2) ◽  
pp. 321-327 ◽  
Author(s):  
Muharni Muharni ◽  
Supriyatna Supriyatna ◽  
Husein H. Bahti ◽  
Dachriyanus Dachriyanus
Keyword(s):  
Antioxidant Activity ◽  
Biological Activity ◽  
Spectroscopic Data ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Reported Data

A phenolic compoud, (-)-epicatechin, was isolated from  the stem bark of Garcinia bancana. The structure of this compound was determined base on spectroscopic  data such as including UV, IR, 1-D, 2-D NMR, and comparison with the reported data. Biological activity of this compound at free radical scavenging activity by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and inhibitory xanthine oxidase(XO) activity showed that (-)- epicathechin active at two methods with IC50 value 8.1 and 8.6 mg/mL respectively.   Keywords: phenolic, (-)-epicatechin, Garcinia  bancana, DPPH, XO

scholarly journals Anti-Inflammatory and Antioxidant Activities ofSalvia fruticosa: An HPLC Determination of Phenolic Contents

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Rima Boukhary ◽  
Karim Raafat ◽  
Asser I. Ghoneim ◽  
Maha Aboul-Ela ◽  
Abdalla El-Lakany
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Aerial Parts ◽  
Phenolic Constituents ◽  
Anti Inflammatory

Objectives.Salvia fruticosaMill. (S. fruticosa) is widely used in folk medicine. Accordingly, the present study was designed to evaluate the antioxidant and anti-inflammatory activities ofS. fruticosa, and to determine the phenolic constituents of its extracts.Methods. The antioxidant activity was determined using 2,2-diphenylpicrylhydrazyl assay. Total phenolic contents were estimated using Folin-Ciocalteu reagent, and high-performance liquid chromatography was performed to identify phenolic constituents. To evaluate the anti-inflammatory activity, carrageenan-induced mouse paw edema was determined plethysmographically.Key Findings. Different plant extracts demonstrated strong radical scavenging activity, where the ethyl acetate extract had the highest value in the roots and the lowest in the aerial parts. This antioxidant activity was correlated to the total phenolic content of different extracts, where rutin and luteolin were the most abundant constituents. Interestingly, both the roots and aerial parts revealed a significant anti-inflammatory activity comparable to diclofenac.Conclusions. This study is the first to demonstrate pharmacologic evidence of the potential anti-inflammatory activity ofS. fruticosa. This activity may partly be due to the radical scavenging effects of its polyphenolic contents. These findings warrant the popular use of the East Mediterranean sage and highlight the potential of its active constituents in the development of new anti-inflammatory drugs.

In vitro Anti-oxidant and Anti-inflammatory Activity of the Aqueous and Ethanol Leaf Extracts of Clerodendrum phlomidis Linn. F.

2021 ◽  
pp. 5217-5221
Author(s):  
Krishnaveni R ◽  
Rajan S.
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Scavenging Activity ◽  
Rbc Membrane ◽  
Clerodendrum Phlomidis ◽  
Anti Oxidant ◽  
Anti Inflammatory

Clerodendrum phlomid is was a common shrub of arid plains, low hills, and tropical deserts. It belongs to the family Verbenaceae. Many species of this genus have been documented in traditional system of medicine in India. The roots of the plant have been extensively studied in Ayurveda but in folk and traditional medicine, it is the leaf that finds more application in alleviating many diseases. Oxidation byproducts of metabolism was responsible for many pathophysiological conditions. Anti-oxidants are molecules that quench the free radicals. The present study aims at evaluating the in-vitro anti-oxidant and anti-inflammatory activity of the aqueous and ethanol leaf extract of Clerodendrum phlomidis. The anti-oxidant activity was evaluated by three in vitro models, namely the DPPH radical scavenging activity, hydroxyl radical scavenging activity and NO radical scavenging activity. The anti-inflammatory activity was evaluated by the RBC membrane Inhibition method. The aqueous extract was found to have better anti- oxidant activity than the ethanol extract in DPPH and NO radical scavenging activity. The RBC membrane inhibition method clearly states that the aqueous leaf extract of Clerodendrum phlomidis was found to have a comparatively an effective anti-inflammatory activity than ethanol extract.

A Preliminary Study on In Vitro Antioxidant and In Vivo Anti-Inflammatory Activity of Cissus quadrangularis Linn.

2021 ◽  
pp. 2619-2624
Author(s):  
Mamta Tiwari ◽  
Pushpraj S Gupta ◽  
Nisha Sharma
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Inflammatory Activity ◽  
Scavenging Activity ◽  
Cissus Quadrangularis ◽  
Anti Inflammatory

Cissus quadrangularis L. is a member of succulent climber of family Vitaceae and it is also called as bone setter because of its bone healing property. The present study focused to assess the in vitro antioxidant and in vivo anti-inflammatory activity of the plant stem extracts in different solvents namely petroleum ether, chloroform, ethyl acetate and methanol. Among all these extracts; ethyl extract showed highest phenolic and flavonoid content (19.35±0.29mg GA/g, 8.68±0.06 RE/g dry wt. respectively). In DPPH free radical scavenging assay all mentioned extracts exhibited free radical scavenging activity but the highest free radical scavenging activity was showed by ethyl acetate extract in increasing dose dependent manner. On the basis of highest phenolic, flavonoid content and free radical scavenging activity ethyl acetate extract was selected for anti- inflammatory activity. The anti-inflammatory activity of Cissus quadrangularis Lin. was investigated in carageenan induced inflammation in Wistar albino rats. Two different doses 250mg/kg and 500mg/kg were administered to the animals. The results showed that both doses had significant reduction in inflammatory condition but dose 500mg/kg showed maximum inflammatory reduction on comparing with control and standard treated groups.

scholarly journals In vitro ANTI-INFLAMMATORY AND ANTIOXIDANT EVALUATION OF AN INDIGENOUS MEDICINAL PLANT – Pterospermum rubiginosum

2021 ◽  
Vol 9 (5) ◽  
pp. 687-696
Author(s):  
Rajamohanan J Anish ◽  
◽  
Arun A Rauf ◽  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Cellular Viability ◽  
Anti Inflammatory

The current study was carried out to determine the antioxidant potential, anti-inflammatory activity, and cellular viability of Pterospermum rubiginosum (PR), a tropical tree endemic to the Western Ghats. The antioxidant activities of the PR bark methanolic (PRME) and aqueous extract (PRAQ) were tested using ABTS as well as superoxide, nitric oxide, and hydroxyl radical assays. Total antioxidant activity was evaluated by adopting the colorimetric method and correlation with their antioxidant activities was derived by Pearson co-efficient analysis. The PRME showed the highest ABTS radical scavenging activity, EC50 (46.09µg/ml) followed by PRAQ (52.08µg/ml). Furthermore, the PRME exhibited the highest scavenging activity against superoxide, nitric oxide, and hydroxyl radicals. The MTT assay results revealed good cellular viability up to a concentration of 100µg/ml with an EC50 (106.869µg/ml). The inflammatory mediators such as Cox-2, IL-1β, IL-6, and NF-kB were reduced during the treatment of PRME in LPS stimulated RAW cells. The stress marker in rat liver cells such as glutathione reductase (GR), glutathione peroxidase (GPx), and reduced glutathione (GSH) levels was found in normal levels when compared to the untreated group of rats. The antioxidant enzyme superoxide dismutase and catalase also exhibited notable bioactivity in PRME treated groups up to a concentration of 1000µg/ml. The present study showed excellent In vitro and In vivo antioxidant activity; the potent anti-inflammatory ability of PRME in reducing the LPS induced inflammation in cell culture conditions.

scholarly journals Ethno-Medicinal Survey of Plants Used in Fever and Biological Activity of Selected Medicinal Plants

2019 ◽  
Vol 5 (1) ◽  
pp. 1-4
Author(s):  
Sunila Paudel ◽  
Khem Raj Joshi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Zone Of Inhibition ◽  
Traditional Treatment ◽  
Ic50 Value ◽  
Keywords Fever

Fifty one plant species belonging to 35 families were documented for the traditional treatment of fever. The widely used species are Centellaasiatica (50%), Ocimumsantum (22.5%), Cissampelospareira (19.5%), Rubusellipticus (10%), and Allium hypsistum (14%). Leaves were used in majority of cases followed by roots in the form of decoction, juice, infusion with or without solvents such as water. Leave extract of R. ellipticus showed potent antioxidant activity towards 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity with IC50 value 2.98 µg/ml compared with standard Ascorbic acid (4.77 µg/ ml). The antibacterial activity of the leave of R. ellipticus showed comparable zone of inhibition with that of standard drugs at a concentration of 1 mg/ml. Keywords: Fever, Antioxidant, Antimicrobial activity, Rubusellipticus, Centellaasiatica

scholarly journals Evaluation of Antioxidant Activity of Synthesized Silver Nanoparticles from Citrus Aurantium Peels Extract by Using the Green Method

2021 ◽  
Vol 16 (2) ◽  
Author(s):  
Sumit Ringwal ◽  
Ankit Singh Bartwal ◽  
S C Nautiyal ◽  
Satish Chandra Sati
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Radical Scavenging Activity ◽  
Research Work ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Citrus Aurantium ◽  
Research Article ◽  
Ic50 Value

The substance which avoids oxidant of easily oxidizing substance even at low concentration is called antioxidant when antioxidant reacts with reactive oxygen species (ROS) or reactive nitrogen species (RNS) they often convert themself into antioxidant radical. Although such a radical has a reduced ability to react with imperative cellular targets, it can still cause damage. To decrease reactivity and reduction potential further down, the ‘antioxidant radical' react with another antioxidant and this reaction continues in a stepwise manner until antioxidant radical’ is no longer damage the cells like lipids, protein, DNA, and other important cellular molecules. In this research article, we had scanned our previously synthesized silver nanoparticles from Citrus aurantium peels extracts; Synthesis and characterization part of the research work is already in communication we are extending our research work on previously synthesized silver nanoparticles. In this research article, we had scanned the antioxidant activity of synthesized AgNPs was determined by H2O2 free radical scavenging activity, we obtained antioxidant activity of BHT with IC50 Value 65.52 µg/mL and antioxidant activity of synthesized AgNPs with IC50 Value 93.92 µg/mL. Antioxidant activity of synthesized AgNPs was also determined by DPPH free radical scavenging activity. We obtained antioxidant activity of BHT with IC50 Value 65.99 µg/mL and antioxidant activity of synthesized AgNPs with IC50 Value 99.68 µg/mL.

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