scholarly journals Rapid Evaluation of Spermidine from 12 Bean Cultivars by Direct Real-Time Mass Spectrometry Analysis

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2138
Author(s):  
Tao Wu ◽  
Xiaoyu Wu ◽  
Xv Yuan ◽  
Yi Wang ◽  
Wenhua Zhou ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Chromatographic Separation ◽  
Extraction Procedure ◽  
Direct Analysis ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Three Samples ◽  
Sample Extraction ◽  
Ionization Mode

The routine spermidine (SPD) detection method is time-consuming and laborious due to the lengthy chromatographic separation and/or tedious sample derivatization pretreatment. In this study, direct analysis in real-time ionization mode coupled with mass spectrometry (DART-MS) was developed to rapidly determine the SPD content of 12 bean cultivars. The results were compared in detail with those of the classical UHPLC-ESI-QTOF method. After conducting a series of optimizations, a simple sample extraction procedure employing 80% aqueous methanol, was followed by determination of sample extracts directly without any chromatographic separation or prior derivatization. The validated method showed excellent performance with low limits of detection (LOD of 0.025 mg·kg−1) and good recovery rates (102.79–148.44%). The investigation highlighted that the DART-MS method (~1.3 min per three samples) could be used as a high-throughput alternative to the classic UHPLC-ESI-QTOF method (~15 min per three samples).

Proteomic and direct analysis in real time mass spectrometry analysis of a Native American ceremonial hat

The Analyst ◽  
2019 ◽  
Vol 144 (24) ◽  
pp. 7437-7446 ◽  
Author(s):  
Timothy P. Cleland ◽  
G. Asher Newsome ◽  
R. Eric Hollinger
Keyword(s):  
Mass Spectrometry ◽  
Native American ◽  
Natural History ◽  
Real Time ◽  
Smithsonian Institution ◽  
Direct Analysis ◽  
Mass Spectrometry Analysis ◽  
National Museum ◽  
Spectrometry Analysis

Complementary mass spectrometry analyses were performed to study a broken ceremonial hat of the Tlingit in the collection of the Smithsonian Institution National Museum of Natural History.

scholarly journals Assessing the feasibility and acceptability of online measurements of exhaled volatile organic compounds (VOCs) in children with preschool wheeze: a pilot study

2021 ◽  
Vol 5 (1) ◽  
pp. e001003
Author(s):  
Karl Holden ◽  
Misty Makinde ◽  
Michael Wilde ◽  
Matthew Richardson ◽  
Tim Coats ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Preschool Children ◽  
Volatile Organic Compounds ◽  
Real Time ◽  
Organic Compounds ◽  
Mass Spectrometry Analysis ◽  
Breathing Patterns ◽  
Spectrometry Analysis ◽  
Non Invasive ◽  
Volatile Organic

BackgroundInvestigating airway inflammation and pathology in wheezy preschool children is both technically and ethically challenging. Identifying and validating non-invasive tests would be a huge clinical advance. Real-time analysis of exhaled volatile organic compounds (VOCs) in adults is established, however, the feasibility of this non-invasive method in young children remains undetermined.AimTo determine the feasibility and acceptability of obtaining breath samples from preschool children by means of real-time mass spectrometry analysis of exhaled VOCs.MethodsBreath samples from preschool children were collected and analysed in real time by proton transfer reaction–time of flight–mass spectrometry (PTR–TOF–MS) capturing unique breath profiles. Acetone (mass channel m/z 59) was used as a reference profile to investigate the breath cycle in more detail. Dynamic time warping (DTW) was used to compare VOC profiles from adult breath to those we obtained in preschool children.Results16 children were recruited in the study, of which eight had acute doctor-diagnosed wheeze (mean (range) age 3.2 (1.9–4.5) years) and eight had no history of wheezing (age 3.3 (2.2–4.1) years). Fully analysable samples were obtained in 11 (68%). DTW was used to ascertain the distance between the time series of mass channel m/z 59 (acetone) and the other 193 channels. Commonality of 12 channels (15, 31, 33, 41, 43, 51, 53, 55, 57, 60, 63 and 77) was established between adult and preschool child samples despite differences in the breathing patterns.ConclusionReal-time measurement of exhaled VOCs by means of PTR–MS is feasible and acceptable in preschool children. Commonality in VOC profiles was found between adult and preschool children.

scholarly journals Serial coupling of chromatographic columns having orthogonal selectivity to improve the coverage of oxidised lipidome for mass spectrometry analysis

2019 ◽  
Author(s):  
Alpesh Thakker ◽  
Andrew Pitt ◽  
Corinne Spickett
Keyword(s):  
Mass Spectrometry ◽  
Chromatographic Separation ◽  
Biological Activities ◽  
Mass Spectrometry Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Hydrophilic Interaction ◽  
Spectrometry Analysis ◽  
Head Groups ◽  
Improved Methods

1AbstractPhospholipid oxidation (OxPL) generates a wide variety of products with potentially novel biological activities that may be associated with disease pathogenesis. To understand their role in disease requires precise information about their abundance in biological samples. Liquid chromatography-mass spectrometry (LCMS) is a sensitive technique that can provide detailed information about the oxidative lipidome, but challenges remain. Furthermore, variation in charge of the polar head groups and the extreme diversity of oxidised species make analysis of several classes of OxPLs within one analytical run challenging.The work in this study aims to develop improved methods for detection of OxPLs by improvement of chromatographic separation through the serial coupling of polystyrene-divinylbenzene based monolithic, and mixed-mode hydrophilic interaction (HILIC) with use of semi-targeted mass spectrometry approaches. The results suggests that by serially coupling two columns, HILIC and monolith, provided the better coverage of OxPL species in a single analytical run. We tested in-vitro generated oxidized species for phosphatidylcholine (PC) and phosphatidylethanolamine (PE) class and the combination of orthogonal chromatographic separation allowed separation of oxdised species from both the classes, which otherwise coeluted.

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