scholarly journals Protective Effect of Cyclically Pressurized Solid–Liquid Extraction Polyphenols from Cagnulari Grape Pomace on Oxidative Endothelial Cell Death

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2105 ◽  
Author(s):  
Anna Posadino ◽  
Grazia Biosa ◽  
Hatem Zayed ◽  
Haissam Abou-Saleh ◽  
Annalisa Cossu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Death ◽  
Biological Activities ◽  
Liquid Extraction ◽  
Grape Pomace ◽  
Ros Generation ◽  
Extraction Technology ◽  
Fluorescence Measurements ◽  
Solid Liquid Extraction ◽  
Solid Liquid

The aim of this work is the evaluation of a green extraction technology to exploit winery waste byproducts. Specifically, a solid–liquid extraction technology (Naviglio Extractor®) was used to obtain polyphenolic antioxidants from the Cagnulari grape marc. The extract was then chemically characterized by spectrophotometric analysis, high-performance liquid chromatography, and mass spectrometry, revealing a total polyphenol content of 4.00 g/L ± 0.05, and the presence of anthocyanins, one of the most representative groups among the total polyphenols in grapes. To investigate potential biological activities of the extract, its ability to counteract hydrogen peroxide-induced oxidative stress and cell death was assessed in primary human endothelial cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, used to assess potential extract cytotoxicity, failed to show any deleterious effect on cultured cells. Fluorescence measurements, attained with the intracellular reactive oxygen species (ROS) probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA), revealed a strong antioxidant potential of the marc extract on the used cells, as indicated by the inhibition of the hydrogen peroxide-induced ROS generation and the counteraction of the oxidative-induced cell death. Our results indicate the Naviglio extraction, as a green technology process, can be used to exploit wine waste to obtain antioxidants which can be used to produce enriched foods and nutraceuticals high in antioxidants.

scholarly journals The Antidiabetic Effect of Grape Pomace Polysaccharide-Polyphenol Complexes

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4495
Author(s):  
Filipa Campos ◽  
Andreia F. Peixoto ◽  
Pedro A. R. Fernandes ◽  
Manuel A. Coimbra ◽  
Nuno Mateus ◽  
...  
Keyword(s):  
Fruits And Vegetables ◽  
Metabolic Diseases ◽  
Liquid Extraction ◽  
Grape Pomace ◽  
Hot Water ◽  
Total Phenolic ◽  
Pectic Polysaccharides ◽  
Antidiabetic Effect ◽  
Solid Liquid Extraction ◽  
Solid Liquid

Type 2 diabetes mellitus (T2DM) is one of the most prevalent chronic metabolic diseases of the 21st century. Nevertheless, its prevalence might be attenuated by taking advantage of bioactive compounds commonly found in fruits and vegetables. This work is focused on the recovery of polyphenols and polysaccharide–polyphenol conjugates from grape pomace for T2DM management and prevention. Bioactives were extracted by solid–liquid extraction and by pressurized hot water extraction (PHWE). Polyphenolic fraction recovered by PHWE showed the highest value for total phenolic content (427 mg GAE.mg−1), mainly anthocyanins and proanthocyanidins, and higher antioxidant activity compared to the fraction recovered by solid–liquid extraction. Polysaccharide–polyphenol conjugates comprehended pectic polysaccharides to which approximately 108 mg GAE of phenolic compounds (per mg fraction) were estimated to be bound. Polyphenols and polysaccharide–polyphenol conjugates exhibited distinct antidiabetic effects, depending on the extraction methodologies employed. Extracts were particularly relevant in the inhibition of a-glucosidase activity, with free polyphenols showing an IC50 of 0.47 mg.mL−1 while conjugates showed an IC50 of 2.7, 4.0 and 5.2 mg.mL−1 (solid–liquid extraction, PHWE at 95 and 120°C, respectively). Antiglycation effect was more pronounced for free polyphenols recovered by PHWE, while the attenuation of glucose uptake by Caco-2 monolayers was more efficient for conjugates obtained by PHWE. The antidiabetic effect of grape pomace bioactives opens new opportunities for the exploitation of these agri-food wastes in food nutrition, the next step towards reaching a circular economy in grape products.

Supercritical fluid and solid–liquid extraction of phenolic antioxidants from grape pomace: a comparative study

2006 ◽  
Vol 226 (1-2) ◽  
pp. 199-205 ◽  
Author(s):  
Manuel Pinelo ◽  
Alejandro Ruiz-Rodríguez ◽  
Jorge Sineiro ◽  
Francisco J. Señoráns ◽  
Guillermo Reglero ◽  
...  
Keyword(s):  
Comparative Study ◽  
Supercritical Fluid ◽  
Liquid Extraction ◽  
Grape Pomace ◽  
Phenolic Antioxidants ◽  
Solid Liquid Extraction ◽  
Solid Liquid

Rotation planar extraction and medium-pressure solid-liquid extraction of onion (Allium cepa)

2003 ◽  
Vol 16 (1) ◽  
pp. 66-70 ◽  
Author(s):  
Irena Vovk ◽  
Breda Simonovska ◽  
Samo Andrenšek ◽  
Teijo Yrjönen ◽  
Pia Vuorela ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Liquid Extraction ◽  
Medium Pressure ◽  
Solid Liquid Extraction ◽  
Solid Liquid

MASS TRANSFER IN SOLID-LIQUID EXTRACTION AT HIGH SOLUTE CONCENTRATIONS

2018 ◽  
Vol 17 (4) ◽  
pp. 827-839
Author(s):  
Eugenia Teodora Iacob Tudose ◽  
Marcela Popa ◽  
Ioan Mamaliga
Keyword(s):  
Mass Transfer ◽  
Liquid Extraction ◽  
Solid Liquid Extraction ◽  
Solid Liquid

Ultrasound Assisted Solid-Liquid Extraction Devices - Harnessing Active Ingredients from Plant materials – Overview and Analysis

2020 ◽  
Vol 13 ◽  
Author(s):  
Venkatasubramanian Sivakumar
Keyword(s):  
Mass Transfer ◽  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Liquid Extraction ◽  
Active Ingredients ◽  
Plant Materials ◽  
Ultrasound Assisted ◽  
Solid Liquid Extraction ◽  
Solid Liquid

Background: In the growing environmental concern use of natural products, efficient processes and devices are necessary. Solid-Liquid extraction of active Ingredients from Plant materials is one of the important unit operations in Chemical Engineering and need to be enhanced. Objectives: Since, these active ingredients are firmly bound to the plant cell wall membrane, which pose mass-transfer resistance and need to get detached through the use of suitable process intensification tools such as ultrasound and suitable devices. Therefore, detailed analysis and review is essential on development made in this area through Publications and Patents. Hence, the present paper illustrates the development of ultrasound assisted device for solid-liquid extraction are presented in this paper. Methods: Advantages such as % Yield, Reduction in extraction time, use of ambient conditions, better process control, avoidance or minimizing multi stage extraction could be achieved due to the use of ultrasound in extraction as compared to conventional processes. Conclusions: Use of ultrasound to provide significant improvements in the extraction of Vegetable tannins, Natural dyes for application in Leather processing has been demonstrated and reported earlier. These enhancement could be possible through various effects of ultrasound such as better flow of solvents through micro-jet formation, mass transfer enhancement due to rupture of plant cell wall membranes through acoustic cavitation, better leaching due to micro-mixing and acoustic streaming effects. This approach would minimize material wastage; thereby, leading to eco-conservation of plant materials, which is very much essential for better environment. Hence, various methods and design for application of ultrasound assisted solid-liquid extractor device are necessary.

Superiority of polymeric scavenger over its hexapodal monomer towards efficient ReO4- removal in water

2021 ◽  
Author(s):  
Pradyut Ghosh ◽  
Rajib Ghosh ◽  
Tamal Kanti Ghosh
Keyword(s):  
Aqueous Medium ◽  
Liquid Extraction ◽  
Efficient Removal ◽  
Solid Liquid Extraction ◽  
Solid Liquid

A new imidazolium functionalized hexapodal polymeric receptor, [PHIm-Br] showed selective and efficient removal (>99%) of perrhenate (ReO4-), from 100% aqueous medium via solid-liquid extraction which was 13% higher as compared...

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