scholarly journals Synthesis, Spectroscopic Analysis and Assessment of the Biological Activity of New Hydrazine and Hydrazide Derivatives of 3-Formylchromone

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2067 ◽  
Author(s):  
Krzysztof Słomiak ◽  
Andrzej Łazarenkow ◽  
Lilianna Chęcińska ◽  
Joachim Kusz ◽  
Justyn Ochocki ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Umbilical Vein ◽  
L929 Cell ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Xtt Assay ◽  
Structural Assessment ◽  
Mouse Fibroblast Cell Line ◽  
Derivatives Of

The hydrazine and hydrazide derivatives of benzo-γ-pyrones with fluorine substituents remain an unexplored group of chemical compounds. This preliminary study reports the synthesis, structural assessment, initial microbiological screening and biological testing of the synthesized compounds on cell lines using the XTT-assay. A series of 10 novel hydrazine and hydrazide derivatives of 3-formylchromone were synthesized and their structures determined. Structural assessment consisted of elemental analysis, IR, 1H-NMR, 13C-NMR, MS and crystallographic studies. Antimicrobial activity was tested on standard strains representing different groups of microorganisms. The tested compounds were found to inhibit microbial growth. Concentrations of 0.01–1250 µmol/L were found to influence cell proliferation, demonstrating antiproliferative and stimulation of proliferation against two cell lines: the L929 cell line (mouse fibroblast cell line) and the EA.hy926 cell line (the human umbilical vein, somatic cell hybrid).

scholarly journals Cytotoxicity and Morphological Effects of Aqueous Areca Nut Crude Extract on L929 Fibroblast Cell Line

2020 ◽  
Vol 4 (1) ◽  
pp. 34-41
Author(s):  
Noor Fadilatul Akmal Mat Salleh ◽  
Ahmad Azlina ◽  
Masitah Hayati Harun ◽  
Badr Abdullah Al-Tayar ◽  
Siti Nurnasihah Md Hashim ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cell Line ◽  
Aqueous Extract ◽  
Crude Extract ◽  
L929 Cell ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Areca Nut ◽  
Fibroblast Cell Line ◽  
Mouse Fibroblast Cell Line

Betel quid chewing is a detrimental recreational habit amongst Asians and a risk factor for oral cancer. Arecoline, a component of areca nut (a major constituent of betel quid) is a known carcinogen. However, the effect of areca nut crude extract is not much studied. To evaluate the cytotoxicity and morphologic effects of areca nut aqueous extract on mouse fibroblast cell line (L929). Dried raw areca nut obtained from a local market in Kota Bharu, Kelantan was prepared and suspended in DMEM (Dulbecco’s Modified Eagle’s medium), prior to serial dilution of 1.56, 0.781, 0.39, 0.195, 0.0976, 0.0488, and 0.0244 mg/ml. The L929 was then exposed to each of the aqueous areca nut extract dilutions and incubated at 37 °C for 24, 48 and 72 hours. Following incubation, the cytotoxicity level of treated L929 was measured using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] assay. Five highest concentrations of areca nut extract showed significantly decreased L929 cell viability (1.56, 0.781, 0.39, 0.195, 0.0976 mg/ml) for all incubation periods compared to untreated cells (p<0.05). The IC50 values of aqueous areca nut extract on L929 were 0.1516, 0.1040, and 0.09136 mg/ml at 24, 48 and 72 hours, respectively. The L929 cell showed altered morphology when cultured in the extract for 24 hours. Higher concentrations of the areca nut aqueous extract is cytotoxic to L929. Prolonged exposure to the extract reduced the IC50 value. Investigation on the role of areca nut in cell proliferation could be further undertaken to assess its association with oral cancer.       Keywords: areca nut, L929, mouse fibroblast cell line, cytotoxicity, MTT assay

Survival and Function of Fibroblasts Stored as Stock Cultures at a Non-freezing Temperature

1993 ◽  
Vol 21 (2) ◽  
pp. 206-209
Author(s):  
Anders H. G. Andrén ◽  
Anders P. Wieslander
Keyword(s):  
Cell Growth ◽  
Cell Line ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Freezing Temperature ◽  
Mouse Fibroblast ◽  
Toxic Response ◽  
Normal Manner ◽  
And Function

Cytotoxicity, measured as inhibition of cell growth of cultured cell lines, is a widely used method for testing the safety of biomaterials and chemicals. One major technical disadvantage with this method is the continuous routine maintenance of the cell lines. We decided to investigate the possibility of storing stock cultures of fibroblasts (L-929) in an ordinary refrigerator as a means of reducing the routine workload. Stock cultures of the mouse fibroblast cell line L-929 were prepared in plastic vials with Eagle's minimum essential medium. The vials were stored in a refrigerator at 4–10°C for periods of 7–31 days. The condition of the cells after storage was determined as cell viability, cell growth and the toxic response to acrylamide, measured as cell growth inhibition. We found that the L-929 cell line can be stored for 2–3, weeks with a viabilty > 90% and a cell growth of about 95%, compared to L-929 cells grown and subcultured in the normal manner. The results also show that the toxic response to acrylamide, using refrigerator stored L-929 cells, corresponds to that of control L-929 cells. We concluded that it is possible to store L-929 cells in a refrigerator for periods of up to 3 weeks and still use the cells for in vitro cytotoxic assays.

Rosette formation by a mouse fibroblast cell line

Nature ◽  
1974 ◽  
Vol 248 (5448) ◽  
pp. 514-515 ◽  
Author(s):  
E. V. ELLIOTT ◽  
R. S. KERBEL ◽  
B. J. PHILLIPS
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Rosette Formation ◽  
Fibroblast Cell Line ◽  
Mouse Fibroblast Cell ◽  
Mouse Fibroblast Cell Line

scholarly journals Evaluation of cytotoxicity of 5-n-alkylresorcinol homologs and fraction on mouse fibroblast cell line L929

2016 ◽  
Vol 243 (7) ◽  
pp. 1137-1148 ◽  
Author(s):  
Izabela Biskup ◽  
Ewa Zaczynska ◽  
Miroslawa Krauze-Baranowska ◽  
Izabela Fecka
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Fibroblast Cell Line ◽  
Mouse Fibroblast Cell ◽  
Mouse Fibroblast Cell Line

Parasiticidal activity of bovine lactoperoxidase against Toxoplasma gondii

2006 ◽  
Vol 84 (5) ◽  
pp. 774-779 ◽  
Author(s):  
Tetsuya Tanaka ◽  
Shin Murakami ◽  
Haruto Kumura ◽  
Ikuo Igarashi ◽  
Kei-ichi Shimazaki
Keyword(s):  
Toxoplasma Gondii ◽  
Cell Line ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
A Cell ◽  
Mouse Fibroblast Cell Line ◽  
Infected Meat ◽  
Free Environment ◽  
Nih 3T3 ◽  
Mouse Macrophage Cell Line

Toxoplasma gondii is an obligatory intracellular parasitic protozoan transmitted via the ingestion of raw, infected meat that causes congenital infections. In a cell-free environment, virulent Toxoplasma was strikingly resistant to H2O2. The activity of H2O2 or H2O2 generated by glucose – glucose oxidase against the resistant tachyzoite stage of pathogenic T. gondii was enhanced by adding KI and bovine lactoperoxidase (bLPO), referred to here as the bLPO system. Replacing bLPO (heme content, 90%) with recombinant bLPO (heme content, 6%) did not enhance the parasiticidal activity with KI and H2O2. These results indicated that heme contributed to the enzyme activity and resulted in the killing of tachyzoites of T. gondii. Tachyzoites treated with the bLPO system also lost the ability to penetrate the mouse fibroblast cell line (NIH/3T3), and could be killed intracellularly after exposure by bLPO to a mouse macrophage cell line (J774A.1). These findings suggested that toxicity was mediated through small amounts of H2O2 generated by phagocytic events in naive macrophages, and by the peroxidative activity of bLPO. Our observations suggest that the bLPO system could help prevent the development of Toxoplasmosis in humans after ingesting raw, infected meat.

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