scholarly journals UPLC-QTOF/MS-Based Metabolomics Applied for the Quality Evaluation of Four Processed Panax ginseng Products

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2062 ◽  
Author(s):  
Jae Lee ◽  
Seung-Heon Ji ◽  
Bo-Ram Choi ◽  
Doo Choi ◽  
Yeong-Geun Lee ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Quality Evaluation ◽  
Principal Component ◽  
Ultra Performance Liquid Chromatography ◽  
Flight Mass Spectrometry ◽  
Black Ginseng ◽  
White Ginseng ◽  
Well Differentiated ◽  
And Control

In the food industry and herbal markets, it is critical to control the quality of processed Panax ginseng products. In this study, ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-QTOF/MS)-based metabolomics was applied for the quality evaluation of white ginseng (WG), tae-geuk ginseng (TG), red ginseng (RG), and black ginseng (BG). Diverse metabolites including ginsenosides were profiled by UPLC-QTOF/MS, and the datasets of WG, TG, RG, and BG were then subjected to multivariate analyses. In principal component analysis (PCA), four processed ginseng products were well-differentiated, and several ginsenosides were identified as major components of each product. S-plot also characterized the metabolic changes between two processed ginseng products, and the major ginsenosides of each product were found as follows: WG (M-Rb1, M-Rb2, M-Rc, Re, Rg1), TG (Rb2, Rc, Rd, Re, Rg1), RG (Rb1, Rb2, Rc, Rd, Re, Rg1), and BG (Rd, Rk1, Rg5, Rg3). Furthermore, the quantitative contents of ginsenosides were evaluated from the four processed ginseng products. Finally, it was indicated that the proposed metabolomics approach was useful for the quality evaluation and control of processed ginseng products.

scholarly journals Correlation between Quality and Geographical Origins of Poria cocos Revealed by Qualitative Fingerprint Profiling and Quantitative Determination of Triterpenoid Acids

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2200 ◽  
Author(s):  
Li-Xia Zhu ◽  
Jun Xu ◽  
Ru-Jing Wang ◽  
Hong-Xiang Li ◽  
Yu-Zhu Tan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Multiple Reaction Monitoring ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Ultra Performance Liquid Chromatography ◽  
Poria Cocos ◽  
Flight Mass Spectrometry ◽  
Geographical Regions ◽  
Array Detection

Poria cocos (Schw.) Wolf (PC) is a well-known saprophytic fungus, and its sclerotium without the epidermis (PCS) is widely used in traditional Chinese medicine and as a functional food in many countries. PCS is normally collected from multiple geographical regions, but whether and how the quality of PCS correlates with where it grows have not been determined. This correlation could be significant both for quality control and optimum utilization of PCS as a natural resource. In this study, a qualitative fingerprint profiling method performed by ultra-performance liquid chromatography (UHPLC) with diode array detection (DAD) combining quadrupole time-of-flight-mass spectrometry (QTOF-MS/MS) and a quantitative UHPLC coupled with triple quadrupole mass spectrometry (QqQ-MS/MS) approach were established to investigate whether and how the quality of PCS correlates with its collection location. A standard fingerprint of PCS was generated by median simulation of 25 tested samples collected from four main producing areas of China, and similarity analysis was applied to evaluate the similarities between the fingerprints of samples and the standard fingerprint. Twenty three common peaks occurring in the fingerprint were unequivocally or tentatively identified by UHPLC-QTOF-MS/MS. Meanwhile, principal component analysis (PCA), supervised orthogonal partial least squares-discriminate analysis (OPLS-DA) and hierarchical cluster analysis (HCA) were employed to classify 25 batches of PCS samples into four groups, which were highly consistent with the four geographical regions. Ten compounds were screened out as potential markers to distinguish the quality of PCS. Nine triterpene acids, including five compounds that played important roles in the clusters between different samples collected from the four collection locations, were simultaneously quantified by using the multiple reaction monitoring (MRM) mode of UHPLC-QqQ-MS/MS. The current strategy not only clearly expounded the correlation between quality and geographical origins of PCS, but also provided a fast, accurate and comprehensive qualitative and quantitative method for assessing the quality of PCS.

scholarly journals Simultaneous determination of various platycosides in Four Platycodon grandiflorum cultivars by UPLC-QTOF/MS

2019 ◽  
Vol 62 (1) ◽  
Author(s):  
Dae Young Lee ◽  
Bo-Ram Choi ◽  
Jae Won Lee ◽  
Yurry Um ◽  
Dahye Yoon ◽  
...  
Keyword(s):  
Principal Component ◽  
Ultra Performance Liquid Chromatography ◽  
Platycodon Grandiflorum ◽  
Pentacyclic Triterpenoid ◽  
Flight Mass Spectrometry ◽  
Platycodi Radix ◽  
Isomeric Pairs ◽  
Different Levels

Abstract In Platycodi Radix (root of Platycodon grandiflorum), there are a number of platycosides that consist of a pentacyclic triterpenoid aglycone and two sugar moieties. Due to the pharmacological activities of platycosides, it is critical to assess their contents in PR, and develop an effective method to profile various platycosides is required. In this study, an analytical method based on ultra performance liquid chromatography coupled with quadrupole time-of-flight/mass spectrometry (UPLC-QTOF/MS) with an in-house library was developed and applied to profile various platycosides from four different Platycodi Radix cultivars. As a result, platycosides, including six isomeric pairs, were successfully analyzed in the PRs. In the principal component analysis, several platycosides were represented as main variables to differentiate the four Platycodi Radix cultivars. Their different levels of platycosides were also represented by relative quantification. Finally, this study indicated the proposed method based on the UPLC-QTOF/MS can be an effective tool for identifying the detail characterization of various platycosides in the Platycodi Radix.

scholarly journals Preparation and Quality Evaluation of Soy Corn Yoghurt

2018 ◽  
Vol 2 ◽  
pp. 102-107
Author(s):  
Suman Lal Shrestha ◽  
Ram Shovit Yadav
Keyword(s):  
Statistical Analysis ◽  
Moisture Content ◽  
Sensory Evaluation ◽  
Storage Time ◽  
Quality Evaluation ◽  
Consumer Acceptability ◽  
Total Solids ◽  
And Control

Soy corn yoghurt is a vegan friendly product prepared by using soymilk as the major ingredient. The aim of this study was to assess the effect of corn milk addition (0, 10, 20, 25 & 30%) on the quality of soy yoghurt. The samples were subjected to sensory evaluation for consumer acceptability and data were subjected to statistical analysis. From sensory evaluation and statistical analysis 10% corn milk inclusion was selected as the best product (p<0.05). The yoghurt thus prepared using 10% corn milk had total solids 22.04%, moisture content 78.62%, protein 4.84%, fat 3.29%, carbohydrate 12.57%, ash 0.81%, acidity 0.68% and pH 4.58. Both control and 10% corn milk incorporated yoghurts were stored under refrigeration 6±1° C and analyzed at 2 days interval till 10 days. Yoghurts with corn milk and control were organoleptically acceptable up to 10 and 6 days respectively. Acidity and syneresis increased but pH decreased with storage time in both samples.

scholarly journals Metabolomics Approach for Discrimination and Quality Control of Natural and Commercial Fallopia multiflora Products in Vietnam

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Nguyen Thi Thoa ◽  
Nguyen Hai Dang ◽  
Do Hoang Giang ◽  
Nguyen Thi Thu Minh ◽  
Nguyen Tien Dat
Keyword(s):  
Clustering Analysis ◽  
Principal Component ◽  
Multivariate Statistical Methods ◽  
Multivariate Statistical ◽  
Fda Guidance ◽  
Promising Tool ◽  
Precision And Accuracy ◽  
And Control

A precise HPLC-DAD-based quantification together with the metabolomics statistical method was developed to distinguish and control the quality of Fallopia multiflora, a popular medicinal material in Vietnam. Multivariate statistical methods such as hierarchical clustering analysis and principal component analysis were utilized to compare and discriminate six natural and twelve commercial samples. 2,3,4′,5-Tetrahydroxystilbene 2-O-β-D-glucopyranoside (THSG) (1), emodin (4), and the new compound 6-hydroxymusizin 8-O-α-D-apiofuranosyl-(1⟶6)-β-D-glucopyranoside (5) could be considered as important markers for classification of F. multiflora. Furthermore, seven phenolics were quantified that the variation in the contents of selected metabolites revealed the differences in the quality of natural and commercial samples. Recovery of the compounds from the analytes was more than 98%, while the limits of detection (LOD) and the limits of quantitation (LOQ) ranged from 0.5 to 6.6 μg/ml and 1.5 to 19.8 μg/ml, respectively. The linearity, LOD, LOQ, precision, and accuracy satisfied the criteria FDA guidance on bioanalytical methods. Overall, this method is a promising tool for discrimination and quality assurance of F. multiflora products.

A Study on the Sound Quality Evaluation Model of Mechanical Air-Cleaners

2009 ◽  
Vol 131 (3) ◽  
Author(s):  
Jeong-Guon Ih ◽  
Su-Won Jang ◽  
Cheol-Ho Jeong ◽  
Youn-Young Jeung
Keyword(s):  
High Frequency ◽  
Quality Evaluation ◽  
Principal Component ◽  
Sound Quality ◽  
Subjective Feeling ◽  
Subjective Data ◽  
Frequency Bands ◽  
Air Cleaner ◽  
Air Cleaners

In operating the air-cleaner for a long time, people in a quiet enclosed space expect low sound at low operational levels for a routine cleaning of air. However, in the condition of high operational levels of the cleaner, a powerful yet nonannoying sound is desired, which is connected to a feeling of an immediate cleaning of pollutants. In this context, it is important to evaluate and design the air-cleaner noise to satisfy such contradictory expectations from the customers. In this study, a model for evaluating the sound quality of air-cleaners of mechanical type was developed based on objective and subjective analyses. Sound signals from various air-cleaners were recorded and they were edited by increasing or decreasing the loudness at three wide specific-loudness bands: 20–400 Hz (0–3.8 barks), 400–1250 Hz (3.8–10 barks), and 1.25–12.5 kHz bands (10–22.8 barks). Subjective tests using the edited sounds were conducted by the semantic differential method (SDM) and the method of successive intervals (MSI). SDM tests for seven adjective pairs were conducted to find the relation between subjective feeling and frequency bands. Two major feelings, performance and annoyance, were factored out from the principal component analysis. We found that the performance feeling was related to both low and high frequency bands, whereas the annoyance feeling was related to high frequency bands. MSI tests using the seven scales were conducted to derive the sound quality index to express the severity of each perceptive descriptor. Annoyance and performance indices of air-cleaners were modeled from the subjective responses of the juries and the measured sound quality metrics: loudness, sharpness, roughness, and fluctuation strength. The multiple regression method was employed to generate sound quality evaluation models. Using the developed indices, sound quality of the measured data was evaluated and compared with the subjective data. The difference between predicted and tested scores was less than 0.5 points.

scholarly journals A New Strategy for Quality Evaluation and Identification of Representative Chemical Components in Polygonum multiflorum Thunb.

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Yun-xia Li ◽  
Xiao-hong Gong ◽  
Mei-chen Liu ◽  
Cheng Peng ◽  
Peng Li ◽  
...  
Keyword(s):  
Quality Evaluation ◽  
Comprehensive Evaluation ◽  
Principal Component ◽  
Chemical Compounds ◽  
Chemical Components ◽  
Health Food ◽  
Polygonum Multiflorum ◽  
New Strategy ◽  
Novel Method

Polygonum multiflorum Thunb. (HSW) is widely used as herb medicine and health food additive. Recently, a series of HSW-induced hepatotoxicities have been reported and many studies have been carried out to investigate it. But contradictory conclusions were drawn that might be caused by the inconsistent quality of market decoction pieces. Therefore, the HSW decoction pieces quality was evaluated with a developed novel method in the paper. 25 batches of raw HSW (RHSW) and 21 batches of processed HSW (PHSW) samples were purchased from different provinces of China. HPLC determination was performed to identify and detect the contents of 16 chemical compounds in herbal material. Fingerprint similarity was analyzed using chromatography information and the results showed that most herbs were in good similarity. Then, a comprehensive evaluation strategy based on principal component analysis with representative quality control indicators was developed to evaluate the quality of HSW samples. And the rationality of the developed method was verified by HCA analysis. The results showed that the herb from Dabashan, Sichuan Province, no matter RHSW or PHSW had the best quality. Different representative components were selected for RHSW or PHSW decoction pieces which might be caused by the chemical reaction during processing. And most PHSW were unqualified according to the requirement of Chinese Pharmacopeia which might take the responsibility for the toxicity of HSW.

scholarly journals Simultaneous Determination of Six Active Compounds in Yixin Badiranjibuya Granules, a Traditional Chinese Medicine, by RP-HPLC-UV Method

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Ning Yu ◽  
ChenHui He ◽  
Gulistan Awuti ◽  
Cheng Zeng ◽  
JianGuo Xing ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Rosmarinic Acid ◽  
Quality Evaluation ◽  
Scientific Basis ◽  
Determination Method ◽  
Rp Hplc ◽  
And Control ◽  
First Time

In this study, a sensitive, precise, and accurate HPLC-UV method was developed and validated to simultaneously determine the six analytes (luteolin-7-O-β-D-glucuronide, apigenin-7-O-β-D-glucuronide, diosmetin-7-O-β-D-glucuronide, acacetin-7-O-β-D-glucuronide, tilianin, and rosmarinic acid) in Yixin Badiranjibuya Granules, in which five analytes (i.e., luteolin-7-O-β-D-glucuronide, apigenin-7-O-β-D-glucuronide, diosmetin-7-O-β-D-glucuronide, acacetin-7-O-β-D-glucuronide, and rosmarinic acid) were determined for the first time in Yixin Badiranjibuya Granules, the content of tilianin in Yixin Badiranjibuya Granules was reported in other literatures, and the content of tilianin in our work was higher than that of the literature reports. The quality of 11 batch samples from four different manufacturers was evaluated using the proposed determination method. The contents of the six analytes were largely different among samples from various manufacturers. Therefore, this determination method can provide a scientific basis for quality evaluation and control of Yixin Badiranjibuya Granules.

scholarly journals Metabolomic Profiling, Antioxidant and Anti-inflammatory Activities of Hypericum Species Growing in South Korea

2017 ◽  
Vol 12 (7) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Heung Joo Yuk ◽  
Kyeong Yeol Oh ◽  
Doo-Young Kim ◽  
Hyuk-Hwan Song ◽  
Jun Young Kim ◽  
...  
Keyword(s):  
Biological Activities ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Principal Component ◽  
Ultra Performance Liquid Chromatography ◽  
Raw 264.7 Cells ◽  
Bioactive Metabolites ◽  
Flight Mass Spectrometry ◽  
Anti Inflammatory ◽  
Hypericum Species

Although Hypericum species are best known as plants that produce hypericin and are used in folk medicine, their other chemical constituents are poorly understood. Polyphenolic secondary metabolites from whole plants of representative Korean Hypericum species ( H. laxum Koidz., H. erectum Thunb., and H. ascyron L.) were analyzed using a ultra performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-Q-TOF/MS)-based approach combined with unsupervised principal component analysis (PCA) multivariate analysis. On the loading scatter plot, significant changes in metabolites were found between species, and three flavonol glycosides (8: quercetin-3- O-galactoside, 12: kaempferol-3- O-glucoside, and 13: quercetin-3- O-rhamnoside) were evaluated as key markers among 17 isolated metabolites. The extracts of H. laxum Koidz. exhibited significant quenching effects on DPPH and ABTS radicals, with IC50 values of 10–20 μg/mL, and were slightly higher in total phenol (TP) and total flavonoid (TF) contents than other species. Additionally, anti-inflammatory activity was observed by reduced nitric oxide (NO) and interleukin-6 (IL-6) production from lipopolysaccharide (LPS)-stimulated macrophages (RAW 264.7 cells). This is the first study to report the presence of bioactive metabolites and their correlating biological activities in H. laxum Koidz.

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