scholarly journals Lignan Glycosides and Flavonoid Glycosides from the Aerial Portion of Lespedeza cuneata and Their Biological Evaluations

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1920 ◽  
Author(s):  
Jiwon Baek ◽  
Tae Lee ◽  
Jae-Hyoung Song ◽  
Eunyong Choi ◽  
Hyun-Jeong Ko ◽  
...  
Keyword(s):  
Negative Control ◽  
Breast Cancer Cell Lines ◽  
Flavonoid Glycosides ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Lespedeza Cuneata ◽  
2D Nmr Spectroscopy ◽  
Aerial Portion ◽  
Alkaline Phosphatase Staining ◽  
Lignan Glycosides

Lespedeza cuneata (Fabaceae), known as Chinese bushclover, has been used in traditional medicines for the treatment of diseases including diabetes, hematuria, and insomnia. As part of a continuing search for bioactive constituents from Korean medicinal plant sources, phytochemical analysis of the aerial portion of L. cuneata led to the isolation of two new lignan glycosides (1,2) along with three known lignan glycosides (3–7) and nine known flavonoid glycosides (8–14). Numerous analysis techniques, including 1D and 2D NMR spectroscopy, CD spectroscopy, HR-MS, and chemical reactions, were utilized for structural elucidation of the new compounds (1,2). The isolated compounds were evaluated for their applicability in medicinal use using cell-based assays. Compounds 1 and 4–6 exhibited weak cytotoxicity against four human breast cancer cell lines (Bt549, MCF7, MDA-MB-231, and HCC70) (IC50 < 30.0 μM). However, none of the isolated compounds showed significant antiviral activity against PR8, HRV1B, or CVB3. In addition, compound 10 produced fewer lipid droplets in Oil Red O staining of mouse mesenchymal stem cells compared to the untreated negative control without altering the amount of alkaline phosphatase staining.

scholarly journals Phytochemical Analysis of the Fruits of Sea Buckthorn (Hippophae rhamnoides): Identification of Organic Acid Derivatives

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 860
Author(s):  
Yong Hoon Lee ◽  
Hee Joo Jang ◽  
Kun Hee Park ◽  
Seon-Hee Kim ◽  
Jung Kyu Kim ◽  
...  
Keyword(s):  
Organic Acid ◽  
Negative Control ◽  
Nutritional Composition ◽  
Sea Buckthorn ◽  
Hippophae Rhamnoides ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Hippophaë Rhamnoides ◽  
The Absolute ◽  
New Compounds

Hippophae rhamnoides L. (Elaeagnaceae), commonly known as “Sea buckthorn” and “Vitamin tree”, is a spiny deciduous shrub whose fruit is known for its nutritional composition, such as vitamin C, and is consumed as a dietary supplement worldwide. As part of our ongoing efforts to identify structurally new and bioactive constituents from natural resources, the phytochemical investigation of the extract of H. rhamnoides fruits led to the isolation of one malate derivative (1), five citrate derivatives (2–6), and one quinate derivative (7). The structures of the isolated compounds were elucidated by analysis of 1D and 2D nuclear magnetic resonance (NMR) spectroscopic data and high-resolution electrospray ionization (HR-ESI) liquid chromatography–mass spectrometry (LC/MS) data. Three of the citrate derivatives were identified as new compounds: (S)-1-butyl-5-methyl citrate (3), (S)-1-butyl-1′-methyl citrate (4), and (S)-1-methyl-1′-butyl citrate (6), which turned out to be isolation artifacts. The absolute configurations of the new compounds were established by quantum chemical electronic circular dichroism (ECD) calculation, which is an informative tool for verifying the absolute configuration of organic acid derivatives. The isolated compounds 1–7 were evaluated for their stimulatory effects on osteogenesis. Compounds 1, 3, 4, 6, and 7 stimulated osteogenic differentiation up to 1.4 fold, compared to the negative control. These findings provide experimental evidence that active compounds 1, 3, 4, 6, and 7 induce the osteogenesis of mesenchymal stem cells and activate bone formation.

Phytochemical evaluation of leaf extracts of Naringi crenulata (roxb.) Nicolson

2016 ◽  
Vol 5 (11) ◽  
pp. 5110
Author(s):  
Sartaj Ahmad Allayie ◽  
Mushtaq Ahmed Parray* ◽  
Bilal Ahmad Bhat ◽  
S. Hemalatha
Keyword(s):  
Quantitative Analysis ◽  
Solvent System ◽  
Great Promise ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Traditional Medicines ◽  
The People ◽  
Wide Range ◽  
Rf Values

The use of traditional medicines holds a great promise as an easily available source as effective medicinal agents to cure a wide range of ailments among the people particularly in tropical developing countries like India. The present study investigates the qualitative and quantitative analysis of the major bioactive constituents of N. crenulata leaf extracts. The extractive values of aqueous, acetone and chloroform extracts were found to be 11.34, 4.24 and 6.06 respectively. Qualitative phytochemical analysis of these three solvent extracts confirm the presence of Alkaloids, Saponins, Flavonoids and Phenolic compounds in all the three extracts; however, these phytochemicals were more significant in aqueous extract. Quantitative analysis was carried out using TLC method by different solvent system. Amongst various solvent systems, Butanol: acetic acid: water (9: 0.9: 0.1 v/v/v) shows maximum resolution and number of spots produced at long UV (365 nm) and under iodine vapours. The TLC chromatograms constituted different coloured phytochemical compounds with different Rf values. It can be conveniently used to evaluate the quality of different area samples. This indicates that the leaves can be useful for treating different diseases because the therapeutic activity of a plant is due to the presence of particular class of compounds and thus can serve as potential sources of useful drugs in future.

scholarly journals Comparative phytochemical profiling and effects of Nerium oleander extracts on the activities of murine peritoneal macrophages

2016 ◽  
Vol 68 (3) ◽  
pp. 515-531 ◽  
Author(s):  
Priyankar Dey ◽  
Tapas Chaudhuri
Keyword(s):  
Free Radical ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Peritoneal Macrophages ◽  
Nerium Oleander ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Root Extracts ◽  
Murine Peritoneal Macrophages

Nerium oleander is a medicinal plant. Apart from its ethnopharmacological uses, pharmacognostic studies have revealed several of its bioactivities. Previously we demonstrated that the phenolic and flavonoid rich extracts of oleander leaf, stem and root possess potent antioxidant and free radical scavenging activities. Moreover, the leaf extract actively modulates the Th1/Th2 cytokine balance and exerts anti-inflammatory activities on murine splenic lymphocytes. Therefore, the present study was designed to evaluate the effect of oleander leaf, stem and root extracts on phagocytosis and the free radical-related activities of murine peritoneal macrophages. In addition, phytochemical profiling was performed using gas chromatography-mass spectrometry (GC-MS). The results demonstrated that the increase in phagocytosis and decrease in myeloperoxidase (MPO) were in the order of leaf>root>stem. The inhibition of cell adhesion, nitric oxide (NO) and elevation of respiratory burst activity was in the order of leaf>stem>root. However, the bioactivities of the leaf extract were much high than those of the stem and root extracts. Phytochemical analysis also revealed the presence of several bioactive constituents in oleander extracts. Therefore, the present study demonstrated that oleander possesses the capacity to modulate macrophage activities and the bioactivities are attributed to the numerous phytochemicals identified in oleander extracts.

In vitro Anthelminthic Activity of Crude Methanolic Leaf Extract of Hyptis suaveolens (L.) Poit (Bush mint) on Ascaris suum.

2019 ◽  
Vol 2 (1) ◽  
pp. 82-87
Author(s):  
O. S Salami
Keyword(s):  
Leaf Extract ◽  
Ascaris Suum ◽  
Positive Control ◽  
Negative Control ◽  
Phytochemical Analysis ◽  
Egg Hatchability ◽  
Anthelminthic Activity ◽  
Life Cycle Stages ◽  
Hyptis Suaveolens

Ascariasis is a neglected tropical disease prevalent in areas with poor hygiene and low standard of living in tropical countries. Hyptis suaveolens is been used traditionally around the world for various ailments. The aim of the study was to evaluate the anthelminthic activity of the methanolic leaf extract of the plant on the three life cycle stages of Ascaris suum synonymous to Ascaris lumbricoides. The phytochemical analysis of the extract was evaluated. The in-vitro anthelminthic activity of the methanolic leaf extract of Hyptis suaveolens on the egg hatchability and larva inhibition of Ascaris suum were assessed using Bizimenyara inhibition method, where 100 eggs were counted and incubated in 5 different plate-wells containing positive control, negative control and 3 concentrations (50, 70 and 100 mg/ml) of extract. The plate wells were brought out after incubation and content examined under the microscope. The effect of the methanolic leaf extract and pyrantel pamoate on cuticle peeling, motility and mortality were evaluated using adult worms. The egg hatchability and larval development after incubation for 48 h and 21 days significantly (p<0.05 for both) inhibited with 98% unhatched eggs and 95% undeveloped larva of A. suum respectively. There was significant (p<0.05) visible peeling of cuticles, reduced motility and mortality of the adult worms after 30, 12 and 80 minutes respectively. It can be concluded that H. suaveolens has anthelminthic activity on A. suum which may be attributed to some of the phytochemical constituents.

Hepatoprotective Effect of Flavonoid Glycosides from Lespedeza cuneata against Oxidative Stress Induced by tert -Butyl Hyperoxide

2011 ◽  
Vol 25 (7) ◽  
pp. 1011-1017 ◽  
Author(s):  
Sang Min Kim ◽  
Kyungsu Kang ◽  
Eun Hye Jho ◽  
Yu-Jin Jung ◽  
Chu Won Nho ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepatoprotective Effect ◽  
Flavonoid Glycosides ◽  
Lespedeza Cuneata ◽  
Tert Butyl

scholarly journals Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of Allium Mongolicum Regel and Their Intestinal Motility Evaluation

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 577 ◽  
Author(s):  
Yongzhe Dong ◽  
Jingya Ruan ◽  
Zhijuan Ding ◽  
Wei Zhao ◽  
Mimi Hao ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Phenolic Acid ◽  
Charge Ratio ◽  
Flavonoid Glycosides ◽  
Fragmentation Pattern ◽  
Bioactive Constituents ◽  
Exact Mass ◽  
Fast Analysis ◽  
Spectrophotometric Methods ◽  
Aerial Parts

To clarify whether flavonoids and phenols in Allium mongolicum Regel have the effect of improving gastrointestinal function and analyze its quality, this study was designed to isolate and identify them from the aerial parts of A. mongolicum by using various chromatographic and spectrophotometric methods, a bioassay on motility of mouse isolated intestine tissue, as well as qualitative analysis using liquid chromatography/mass spectrometry (LC-MS) analysis. As a result, 31 flavonoids and phenolic acids were obtained and identified, including six new flavonoid glycosides, mongoflavonosides A1 (1), A2 (2), A3 (3), A4 (4), B1 (5), B2 (6), and four new phenolic acid glycosides, mongophenosides A1 (7), A2 (8), A3 (9), B (10). Among them, eleven flavonoids and three phenolic acids showed significant increase in the height of mouse small intestinal muscle. It was a first systematic bioactive constituents’ study for A. mongolicum on gastrointestinal tract. Furthermore, according to the retention time (tR) and the exact mass-to-charge ratio (m/z), thirty-one compounds were unambiguously identified by comparing to the standard references by using LC-MS. Then, on the basis of generalized rules of MS/MS fragmentation pattern, chromatographic behaviors, as well as biosynthetic laws of the 31 isolates, five flavonoid glycosides and one phenolic acid glycoside were tentatively speculated. On the basis of the study, a fast analysis method for flavonoids and phenolic acids in A. mongolicum was established.

scholarly journals IN VITRO EVALUATION OF THE ANTHELMINTIC ACTIVITY OF RHIZOME EXTRACTS OF CURCUMA LONGA (LINN.)

2018 ◽  
Vol 11 (12) ◽  
pp. 425
Author(s):  
Jyoti Pandey ◽  
Suman Mishra ◽  
Kamal Jaiswal
Keyword(s):  
Curcuma Longa ◽  
Anthelmintic Activity ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Alternative Source ◽  
Significant Efficacy ◽  
Phosphate Buffered Saline

Objective: The current study was carried out to evaluate the anthelmintic activity of the rhizome extract of Curcuma longa as an alternative source of effective remedies for nematodiasis.Methods: The anthelmintic activity of the C. longa was assessed in vitro against Haemonchus spp., a gastrointestinal (abomasum) parasite of goats. Different concentrations of the extracts (1 mg/mL, 2.5 mg/mL, 5 mg/mL, and 10 mg/mL) in phosphate-buffered saline (PBS) were tested, and the results expressed in terms of time of paralysis (minute) and time of death (minute) of the worms. Albendazole (1 mg/mL) was used as a reference (positive control) and PBS as a control group (negative control).Results: The qualitative phytochemical analysis of the methanolic extract (ME) of the plant disclosed the presence of alkaloids, glycosides, terpenoids, flavonoids, tannins, saponins, phenol, anthraquinone, and carbohydrates; whereas, the aqueous extract (AE) showed the presence of alkaloids, carbohydrate, flavonoids, and saponins. Both ME and AE of the C. longa (rhizome) expressed significant efficacy (p≤0.05) in causing paralysis as well as the death of the worms within 12 h of exposure at all tested concentrations, as compared to the negative control. The rhizome extracts of C. longa showed dose-dependent efficacy in causing paralysis of the worm motility and the final progression to death. The results showed that the ME at 10 mg/mL was significantly more potent (p≤0.05) over the AE.Conclusions: This study concluded that the rhizome extract of C. longa exhibited potent anthelmintic efficacy against the nematode parasite, Haemonchus spp.

Phenylpropanoid and lignan glycosides from the aerial parts of Lespedeza cuneata

2016 ◽  
Vol 121 ◽  
pp. 58-64 ◽  
Author(s):  
Jian Zhou ◽  
Chuang-Jun Li ◽  
Jing-Zhi Yang ◽  
Jie Ma ◽  
Lian-Qiu Wu ◽  
...  
Keyword(s):  
Lespedeza Cuneata ◽  
Aerial Parts ◽  
Lignan Glycosides

scholarly journals PRELIMINARY PHYTOCHEMICAL STUDIES OF SELECTED MEDICINAL PLANT PACHYGONE OVATA (POER.) HOOK.F. & THOMS FROM MENISPERMACEAE FAMILY FOR BIOACTIVE CONSTITUENTS

2018 ◽  
Vol 5 (2) ◽  
pp. 50-52
Author(s):  
Renjini Haridas ◽  
Radhakrishnan G ◽  
Reshma R ◽  
Sumathi P
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Cardiac Glycosides ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Phytochemical Studies ◽  
Leaf Part ◽  
Important Medicinal Plant

The present study deals with the phytochemical examination of Pachygone ovata (Poer.) Hook.f.& Thoms., an important medicinal plant from menispermaceae family. Leaf and Stem extracts were prepared by using different solvents systems and phytochemical screening was performed using the standard methods given by Harborne. Leaf and stem extracts were prepared from aqueous and organic solvents like petroleum ether, acetone, ethyl acetate and ethanol. Qualitative phytochemical analysis of the petroleum ether, acetone,ethyl acetate, ethanol and aqueous extracts prepared from P. ovata leaf and stem part. Leaf part revealed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, phenols and tannins. Stem part revealed the presence of alkaloids, flavonoids, glycosides, Resin, Steroids, phenols and tannins. The ethanolic extract showed higher amount of secondary metabolites than the other solvent extracts. This observation becomes important in the context of the therapeutically and drug applications of P. ovata.

scholarly journals Evaluation of qualitative phytochemicals and antifungal activity of aqueous leaf extract of Senna tora on downy mildew of cabbage

2018 ◽  
Vol 11 (1) ◽  
pp. 15-19
Author(s):  
S.M. Zakari ◽  
A.A. Aliko ◽  
H Haruna ◽  
A Muazu
Keyword(s):  
Downy Mildew ◽  
Leaf Extract ◽  
Flavonoid Glycosides ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Active Metabolites ◽  
Leaf Extracts ◽  
Minimum Fungicidal Concentration ◽  
Significant Difference ◽  
Aqueous Leaf Extract

This study was embarked upon to evaluate the efficacy of aqueous extracts of Senna tora leaf against Perenospora parasitica, causing downy mildew on cabbage. Phytochemical analysis of the leaf extracts revealed the presence of some secondary metabolites (Tannins, Saponins, Flavonoid, Glycosides, Alkaloids, Phenolic compounds and Carbohydrates) which have been reported to have antimicrobial effects. Assays were performed using extract concentrations of 1000, 500, 250, 125 mg/ml by agar well diffusion technique. Results obtained, revealed a significant difference in diameter of mycelial zone of inhibition between the concentrations. Highest zone of inhibition was recorded at 1000 and 500 mg/ml (20 and 15 mm) respectively. Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) were determined and recorded at 250 mg/ml. The antifungal screening revealed that the extract exhibited inhibitory activities at varying concentrations against the downy mildew pathogen. These activities observed could be attributed to the presence of active metabolites contained in the extract.Keywords: Aqueous leaf extract, Downy mildew, Perenospora parasitica and Senna tora

Sign in / Sign up

Export Citation Format

Share Document