scholarly journals Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of Platycodi Radix Collected from Various Regions in China by HPLC Coupled with Chemometrics

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1823 ◽  
Author(s):  
Haiyang Lu ◽  
Mengzhen Ju ◽  
Shanshan Chu ◽  
Tao Xu ◽  
Yuzhe Huang ◽  
...  
Keyword(s):  
Quality Evaluation ◽  
Southern China ◽  
Pulmonary Diseases ◽  
Evaporative Light Scattering Detection ◽  
Fingerprint Analysis ◽  
Light Scattering Detection ◽  
Platycodi Radix ◽  
Evaporative Light Scattering ◽  
Long Time

Platycodi Radix (PR) is the root of Platycodon grandiflorum (Jacq.) A. DC., which has been used for a long time in China to treat pulmonary diseases. The present study aimed to evaluate the quality of PR samples collected from 23 regions of 11 provinces in China. Eight saponins were quantified using HPLC coupled with evaporative light scattering detection (HPLC-ELSD). The samples with the highest total contents of saponins were from southern China, such as Yunnan, Guangxi, Jiangxi, and Guangzhou. The fingerprint analysis of PR samples was conducted by HPLC-UV method. Nineteen common peaks were selected and the similarity values varied from 0.607 to 0.921. These findings indicated that the saponins contents of PR from different regions varied significantly, with PR samples from southern China having the highest contents of saponins. These comprehensive methods were successful in evaluating the quality of PR samples from northern and southern China, which will serve as a guide for the development of PR as a clinical medication.

scholarly journals Chromatographic fingerprints and quantitative analysis for the quality evaluation of Bao-Yuan Decoction by ultra-performance liquid chromatography coupled with diode array and evaporative light scattering detection

2021 ◽  
Author(s):  
Min Zhang ◽  
Zhenling Zhu ◽  
Mengyue Wang ◽  
Jincai Hou ◽  
Yinqing Li ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Light Scattering ◽  
Ultra Performance Liquid Chromatography ◽  
Dosage Forms ◽  
Diode Array ◽  
Evaporative Light Scattering Detection ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering ◽  
Marker Compounds

AbstractBao-Yuan Decoction (BYD), a widely used traditional Chinese medicine formula, is worth developing into modern dosage forms. To assess the quality of traditional decoction, the commonly used ultra-performance liquid chromatography coupled with diode array and evaporative light scattering detection (UPLC-DAD/ELSD) method was initially applied to develop the analytical methods for the qualitative fingerprints and simultaneous quantitation of multiple marker compounds in BYD. Based on 16 batches of BYD prepared from multiple batches of qualified crude herbs combined randomly, the characteristic fingerprints were generated, with 41 and 19 common peaks detected by DAD and ELSD, respectively. Furthermore, ginsenosides Re, Rg1 and Rb1, calycosin-7-glucoside, calycosin, liquiritin, isoliquiritin apioside, isoliquiritin, glycyrrhizic acid and cinnamic acid were qualified as marker compounds to represent the herbs composing the formula. The characteristic fingerprints and the content ranges of multiple batches of the decoction were obtained, thus providing guidance for the quality control of modern dosage forms. The combination of these qualitative and quantitative methods will be an effective operational measure by which to evaluate and control the quality of BYD from traditional decoction to modern dosage forms.

scholarly journals Identification and Quantification of three Tubeimosides in Rhizoma Bolbostematis by High Performance Liquid Chromatography with Evaporative Light Scattering Detection and Electrospray Mass Spectrometric Detection

2008 ◽  
Vol 3 (5) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Hao Huang ◽  
MingJin Liang ◽  
Wen Xu ◽  
Chuan Zhang ◽  
WeiDong Zhang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Light Scattering ◽  
High Performance ◽  
Methanol Extract ◽  
Gradient Elution ◽  
Evaporative Light Scattering Detection ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering

Tubeimoside I, tubeimoside II and tubeimoside III were simultaneously determined and identified by high performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC-ESI/MS), and a novel and sensitive high performance liquid chromatography - evaporative light scattering detection (HPLC-ELSD) in a 70% methanol extract of Rhizoma Bolbostematis. The chromatographic separation was performed on a Zorbax Extend C18 analytical column using gradient elution with a solution of acetonitrile and 0.5% acetic acid. The method was validated with acceptable linearities (r > 0.9992) and recoveries (98.6 to 102.4 %). The limits of detection of these three tubeimosides were as low as 0.05 μg. The intra- and inter-day precisions of the method were evaluated and were less than 3.6%. The method was successfully used to analyze 15 batches of Rhizoma Bolbostematis. The content of tubeimosides in the plant material varied from habitat to habitat confirming the necessity to control the quality of Rhizoma Bolbostematis during its preparation and application in the clinic.

Fingerprint Analysis of Flos Lonicerae by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

2012 ◽  
Vol 554-556 ◽  
pp. 2058-2063
Author(s):  
Feng Lai Lu ◽  
Chuan Ming Fu ◽  
Hai Ying Jiang ◽  
Yue Yuan Chen ◽  
Jin Lei Liu ◽  
...  
Keyword(s):  
Light Scattering ◽  
Flow Rate ◽  
Retention Time ◽  
High Performance ◽  
Evaporative Light Scattering Detection ◽  
Fingerprint Analysis ◽  
Light Scattering Detection ◽  
Flos Lonicerae ◽  
Evaporative Light Scattering ◽  
Relative Standard

A high-performance liquid chromatographic method (HPLC) with evaporative light scattering detection (ELSD) has been developed for fingerprint analysis of Flos Lonicerae. The samples were separated with an Agilent C18 column using acetonitrile (A) and 0.1% formic acid solution under gradient conditions (0→10 min：11.5%→15% A, 10→12 min：15% →22% A, 12→18min：22%→33% A; 18→30min: 33%→45% A) as the mobile phase at a flow rate of 0.8ml min−1 within 30 min. The ELSD conditions were optimized at nebulizer-gas flow rate 2.7 L min−1 and drift tube temperature 105°C. Precision experiments showed relative standard deviation (R.S.D.) of peak area and retention time were better than 2.5%, inter-day and intra-day variabilities showed that R.S.D. was ranged from 0.66% to 4.17%. Accuracy validation showed that average recovery was between 95.33% and 104.10%. The method was validated to achieve the satisfactory precision and recovery. Relative retention time and relative peak area were used to identify the common peaks for fingerprint analysis. There are nine common peaks in the fingerprint. The quality of sixteen batches of Flos Lonicerae samples was evaluated by hierarchical clustering analysis, which classfied all bacthes into two clusters. Furthermore, the contents of important medicinal compounds (chlorogenic acid, macranthoidin B and dipsacoside B) in different batches of Flos Lonicerae samples were determined simultaneously using the developed HPLC-ELSD method. The developed analytical procedure was proved to be a reliable and rapid method for the quality control of Flos Lonicerae.

scholarly journals Chemical Fingerprint Analysis and Quantitative Analysis of Saccharides in Morindae Officinalis Radix by HPLC-ELSD

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7242
Author(s):  
Hongmei Sun ◽  
Yini Cai ◽  
Jie Shen ◽  
Enyao Ma ◽  
Zhimin Zhao ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Ion Mobility ◽  
High Performance ◽  
Gradient Elution ◽  
Fingerprint Analysis ◽  
Chemical Fingerprint ◽  
Hplc Fingerprint ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering

A method based on high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for the quantitative analysis of three active compounds and chemical fingerprint analyses of saccharides in Morindae officinalis radix. Ten batches of Morindae officinalis radix were collected from different plantations in the Guangdong region of China and used to establish the fingerprint. The samples were separated with a COSMOIL Sugar-D column (4.6 mm × 250 mm, 5 μm) by using gradient elution with water (A) and acetonitrile (B). In addition, Trapped-Ion-Mobility (tims) Time-Of-Flight (tims TOF) was used to identify saccharides of Morindae officinalis radix. Fingerprint chromatogram presented 26 common characteristic peaks in the roots of Morinda officinalis How, and the similarities were more than 0.926. In quantitative analysis, the three compounds showed good regression (r = 0.9995–0.9998) within the test ranges, and the recoveries of the method were in the range of 96.7–101.7%. The contents of sucrose, kestose and nystose in all samples were determined as 1.21–7.92%, 1.02–3.37%, and 2.38–6.55%, respectively. The developed HPLC fingerprint method is reliable and was validated for the quality control and identification of Morindae officinalis radix and can be successfully used to assess the quality of Morindae officinalis radix.

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