scholarly journals Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1725 ◽  
Author(s):  
Zhe Chen ◽  
Zhaoyang Ding ◽  
Guangya Zhang ◽  
Leilei Tian ◽  
Xuanjun Zhang
Keyword(s):  
Fluorescence Intensity ◽  
Gel Electrophoresis ◽  
Emission Spectroscopy ◽  
Water Solubility ◽  
Fluorescence Emission ◽  
Amide Bond ◽  
Aggregation Induced Emission ◽  
Bio Imaging ◽  
High Fluorescence Intensity ◽  
High Fluorescence

In this work, an aggregation-induced emission (AIE) molecule (tetraphenylethene derivative, TPE-COOH) was conjugated to elastin-like polypeptides (ELPs40) via an amide bond to form ELPs40-TPE. The successful synthesis of ELPs40-TPE was confirmed by Circular Dichroism spectroscopy, gel electrophoresis, UV-vis absorption, and fluorescence emission spectroscopy. ELPs40-TPE possessed both amphiphilicity and the features of an AIE, and the fluorescence intensity was dependent on the local temperature. The Hela cells imaging indicated that ELPs40-TPE has great potential for bio-imaging applications because of its advantages of high fluorescence intensity, good water-solubility, and remarkable biocompatibility.

Synthesis, aggregation-induced emission and electroluminescence properties of three new phenylethylene derivatives comprising carbazole and (dimesitylboranyl)phenyl groups

2017 ◽  
Vol 5 (45) ◽  
pp. 11741-11750 ◽  
Author(s):  
Heping Shi ◽  
Xinlei Zhang ◽  
Chen Gui ◽  
Shujuan Wang ◽  
Li Fang ◽  
...  
Keyword(s):  
Solid State ◽  
Fluorescence Intensity ◽  
Light Emitting Diodes ◽  
Organic Light Emitting Diodes ◽  
Light Emitting ◽  
Aggregation Induced Emission ◽  
Organic Light ◽  
High Fluorescence Intensity ◽  
High Fluorescence

It is essential that light-emitting materials possess high fluorescence intensity in the solid-state and a stable charge-transporting ability for the construction of organic light-emitting diodes (OLEDs) with outstanding performance.

L-Histidine Stabilized Copper Nanoclusters as a Fluorescence Probe for Determination of Fluazinam

NANO ◽  
2020 ◽  
Vol 15 (05) ◽  
pp. 2050063
Author(s):  
Zhifeng Cai ◽  
Xiu Yin ◽  
Jingling Fang ◽  
Jie Zhao ◽  
Tianqi Wu ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Photoelectron Spectroscopy ◽  
Water Solubility ◽  
Fluorescence Probe ◽  
Synthesis Method ◽  
Fluorescence Emission ◽  
Average Diameter ◽  
High Dispersion ◽  
Excitation Wavelength ◽  
One Pot

In this contribution, a one-pot synthesis method possessing the advantages of simple, green and low-cost had been researched for the preparation of L-histidine-stabilized Cu nanoclusters (Cu NCs). Subsequently, the structure and optical properties of as-prepared Cu NCs were studied by using Fourier transform infrared (FTIR), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), fluorescence spectroscopy and UV-Vis absorption spectroscopy. TEM image of the Cu NCs showed high dispersion with an average diameter of 2.0[Formula: see text]nm. Fluorescence spectrum displayed that the Cu NCs emitted green fluorescence (emission wavelength of 492[Formula: see text]nm) under excitation wavelength of 393[Formula: see text]nm. Moreover, the as-synthesized Cu NCs illustrated excellent performances, such as good water solubility, UV stability and high-salt resistance. Interestingly, the fluorescence intensity of as-prepared Cu NCs was obviously quenched in the presence of fluazinam. Under optimal conditions, the relative fluorescence intensity was linear with the fluazinam concentrations from 1 to 40[Formula: see text][Formula: see text]M, with a detection limit of 0.25[Formula: see text][Formula: see text]M. Eventually, the fluorescence sensor was successfully used to determine fluazinam in real water samples.

scholarly journals A flow cytometric assay of neutrophil degranulation.

1983 ◽  
Vol 31 (6) ◽  
pp. 737-744 ◽  
Author(s):  
W R Abrams ◽  
L W Diamond ◽  
A B Kane
Keyword(s):  
Flow Cytometry ◽  
Fluorescence Intensity ◽  
Ionophore A23187 ◽  
Flow Cytometric Assay ◽  
Elastase Activity ◽  
Flow Cytometric ◽  
Specific Granules ◽  
Neutrophil Degranulation ◽  
High Fluorescence Intensity ◽  
High Fluorescence

A quantitative assay of neutrophil degranulation was developed using flow cytometry. Dog neutrophils were purified to greater than 95% purity and viability by isopyknic density centrifugation in an isosmotic medium. These cells concentrated the fluorochrome acridine orange (AO) in their azurophilic granules, but not in specific granules. Also contained in the azurophilic granules are elastase, myeloperoxidase, and approximately 50% of the lysozyme activity. The fluorochrome was released concomitantly with elastase activity, as shown by flow cytometry, fluorescence microscopy, and biochemical assay in response to the ionophore A23187. By flow cytometry, unstimulated cells are distributed in a single broad peak of high fluorescence intensity. With increasing concentrations of A23187 (0.48-4.80 microM), a greater proportion of the cells shifted to a single peak of low fluorescence intensity. Few cells with intermediate fluorescence were observed. These analyses revealed that the neutrophils degranulated in a quantal, all-or-none response.

scholarly journals Copolymerization of Eu(TTA)3Phen doped styrene and methyl methacrylate nanoparticles and use in quantitative detection of pepsinogen

RSC Advances ◽  
2017 ◽  
Vol 7 (20) ◽  
pp. 12217-12223 ◽  
Author(s):  
Feng Wu ◽  
Mao Mao ◽  
Yu Cen ◽  
Hongtian Yang ◽  
Zhifeng Qin ◽  
...  
Keyword(s):  
Methyl Methacrylate ◽  
Fluorescence Intensity ◽  
Quantitative Detection ◽  
High Fluorescence Intensity ◽  
High Fluorescence

High fluorescence intensity nanoparticles were prepared by copolymerization of Eu(TTA)3Phen doped styrene and methyl methacrylate.

Benzothiazole integrated into a cryptand for ESIPT-based selective chemosensor for Zn2+ ions

2019 ◽  
Vol 48 (22) ◽  
pp. 7801-7808 ◽  
Author(s):  
Mayank Gupta ◽  
Sunanda Sahana ◽  
Vivekanand Sharma ◽  
Parimal K. Bharadwaj
Keyword(s):  
Excited State ◽  
Detection Limit ◽  
Proton Transfer ◽  
Fluorescence Intensity ◽  
Intramolecular Proton Transfer ◽  
High Fluorescence Intensity ◽  
High Fluorescence

A novel 2(2′-hydroxyphenyl) benzothiazole-based cryptand (L) exhibits high fluorescence intensity in the presence of Zn2+ ions by stopping the excited state intramolecular proton transfer (ESIPT) process with a detection limit of 0.20 μM.

scholarly journals Synthesis and Optical Characterizations of the Fluorescence Silica Nanoparticles Containing Quantum Dots

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Chu Viet Ha ◽  
Chu Anh Tuan ◽  
Nguyen Thi Bich Ngoc ◽  
Tran Hong Nhung ◽  
Nguyen Quang Liem ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Silica Nanoparticles ◽  
Water Solubility ◽  
Fluorescence Emission ◽  
Ammonium Hydroxide ◽  
Sio2 Nanoparticles ◽  
Synthesis Conditions ◽  
High Fluorescence ◽  
Modified Stöber Method ◽  
Fluorescence Material

The quantum dots coated by silica  is fluorescence material class with great biocompatibility, low toxicity and water-solubility, that is suitable for bioapplications. This work presents the synthesis of SiO2 coated CdTe/ZnSe (named CdTe) quantum dots (CdTe@SiO2 nanoparticles) via a wet chemmical route called modified Stöber method. The compounds tetraethylorthosilicate (TEOS) has used as precursors, aminopropyltriethoxysilane (APTES) is  as  electric neutralizer, and ammonium hydroxide is used as catalysts. The size of CdTe@SiO2 nanoparticles was estimated about 70 to 150 nm depending on the quantities of H2O, APTEOS, and catalysts. The emission behaviours of SiO2 coated quantum dots was effected by ratio of substances participating in the reaction and synthesis conditions. with the ratio (by volume) of suitable substances: TEOS:solution of QDs:NH4OH:APTES:H2O being 1.5:1.5x10-2:0.8x10-2:4x10-2:3x10-4:5x10-2, the prepared silica nanoparticles containing quantum dots show high fluorescence emission efficiency, the fluorescence intensity is higher than that of uncoated CdTe/ZnSe quantum dots. This is a positive result in the technique of manufacturing luminescent silica nanoparticles containing quantum dots. The results show an ability to use the CdTe@SiO2 nanoparticles for biological application.

scholarly journals Detection of Counterfeit Tequila by Fluorescence Spectroscopy

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
José Manuel de la Rosa Vázquez ◽  
Diego Adrián Fabila-Bustos ◽  
Luis Felipe de Jesús Quintanar-Hernández ◽  
Alma Valor ◽  
Suren Stolik
Keyword(s):  
Fluorescence Spectroscopy ◽  
Fluorescence Intensity ◽  
Uv Light ◽  
Fluorescence Emission ◽  
Good Method ◽  
Fluorescence Signal ◽  
Fluorescence Measurement ◽  
Fluorescence Study ◽  
Light Emitting ◽  
High Fluorescence

An ultraviolet (UV) light induced fluorescence study to discriminate fake tequila from genuine ones is presented. A portable homemade system based on four light emitting diodes (LEDs) from 255 to 405 nm and a miniature spectrometer was used. It has been shown that unlike fake and silver tequila, which produce weak fluorescence signal, genuine mixed, rested, and aged tequilas show high fluorescence emission in the range from 400 to 750 nm. The fluorescence intensity grows with aging in 100% agave tequila. Such fluorescence differences can even be observed with naked eyes. The presented results demonstrate that the fluorescence measurement could be a good method to detect counterfeit tequila.

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