scholarly journals Effect of Dibutyltin Dilaurate on Triglyceride Metabolism through the Inhibition of the mTOR Pathway in Human HL7702 Liver Cells

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1654 ◽  
Author(s):  
Xiaozhi Qiao ◽  
Yunlan Li ◽  
Jiaqi Mai ◽  
Xiaoqing Ji ◽  
Qingshan Li
Keyword(s):  
Liver Cells ◽  
Mtor Pathway ◽  
Dibutyltin Dilaurate ◽  
Triglyceride Metabolism

Abstract 30: Apolipoprotein CIII Sialylation is a Critical Determinant of Liver Triglyceride Metabolism

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Hussein N Yassine ◽  
Ambika Ramrakhiani ◽  
Aarushi Parekh ◽  
Ryan Walker ◽  
Michael Goran ◽  
...  
Keyword(s):  
Plasma Concentrations ◽  
Liver Cells ◽  
Liver Fat ◽  
Lipid Uptake ◽  
Liver Uptake ◽  
Critical Determinant ◽  
Triglyceride Metabolism ◽  
Apo E

Apolipoprotein (apo) CIII inhibits lipoprotein lipase (LPL) activity and remnant particle uptake in the liver by the lipolysis stimulated receptor (LSR); and therefore modulates liver and systemic lipid metabolism. Although post-translational modifications of apo CIII lead to asialylated, mono- or di-sialylated variants, the biological relevance of these sialylations is not known. Our objectives were to determine the association of apo C-III sialylation with plasma triglycerides and liver fat content in vivo, and to evaluate the effect of apo CIII sialylation on lipid uptake in liver cells or LPL activity in vitro. In 209 obese non-diabetic adolescent Hispanic participants, apo CIII variants in plasma were measured using mass spectrometric immunoassay. Increased plasma apo C-III sialylation (di- to mono-sialylated apo CIII ratio) was associated with lower triglyceride levels (r=-0.43, p<0.001) and liver fat (by MRI, r=-0.27, p<0.001) independent of total apo CIII concentrations. Higher plasma concentrations of the mono-, but not the di-sialylated variant, were associated with higher triglycerides concentrations (r=0.53, p<0.001). In HepG2 liver cells, immunoprecipitated apo CIII from VLDL of participants in the upper quartile of plasma apo CIII sialylation less effectively inhibited LSR-mediated VLDL uptake compared to apo CIII from those in the lower quartile of apo CIII sialylation (28% difference, p=0.02). This difference in inhibition was abolished by removal of sialic acid with neuraminidase. Apo CIII isolated from VLDL of participants with different level of apo CIII sialylation showed similar capacity to inhibit fatty acid release by LPL of control VLDL. VLDL particles with higher apo CIII sialylation also demonstrated a two-fold increase in ApoE. We conclude that a greater apo CIII sialylation together with particle enrichment in apo E allow for more efficient VLDL liver uptake and less liver fat accumulation. The measurement of plasma apo CIII sialylation may be a useful index of triglyceride metabolism and risk of fatty liver.

The Morphology of Vacuolated Cells in the Liver Following Administration of Vitamin A.

1973 ◽  
Vol 31 ◽  
pp. 408-409
Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo ◽  
Fawzia Batti
Keyword(s):  
Electron Microscopy ◽  
Body Weight ◽  
Vitamin A ◽  
Light And Electron Microscopy ◽  
Liver Cells ◽  
Prominent Feature ◽  
Vascular Space ◽  
Vacuolated Cell ◽  
Vacuolated Cells ◽  
Vacuolated Cytoplasm

Vacuolated cells in the liver of young rats were studied by light and electron microscopy following the administration of vitamin A (200 units per gram of body weight). Their characteristics were compared with similar cells found in untreated animals.In rats given vitamin A, cells with vacuolated cytoplasm were a prominent feature. These cells were found mostly in a perisinusoidal location, although some appeared to be in between liver cells (Fig. 1). Electron microscopy confirmed their location in Disse's space adjacent to the sinusoid and in recesses between liver cells. Some appeared to be bordering the lumen of the sinusoid, but careful observation usually revealed a tenuous endothelial process separating the vacuolated cell from the vascular space. In appropriate sections, fenestrations in the thin endothelial processes were noted (Fig. 2, arrow).

Effects of Hypophysectomy on the Fine Structure of Rat Liver Cells

1967 ◽  
Vol 25 ◽  
pp. 156-157
Author(s):  
Robert R. Cardell
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Rat Liver ◽  
Liver Cell ◽  
Anterior Pituitary ◽  
Liver Cells ◽  
Biochemical Studies ◽  
Liver Functions ◽  
Rat Liver Cells ◽  
And Control

Hypophysectomy of the rat renders this animal deficient in the hormones of the anterior pituitary gland, thus causing many primary and secondary hormonal effects on basic liver functions. Biochemical studies of these alterations in the rat liver cell are quite extensive; however, relatively few morphological observations on such cells have been recorded. Because the available biochemical information was derived mostly from disrupted and fractionated liver cells, it seemed desirable to examine the problem with the techniques of electron microscopy in order to see what changes are apparent in the intact liver cell after hypophysectomy. Accordingly, liver cells from rats which had been hypophysectomized 5-120 days before sacrifice were studied. Sham-operated rats served as controls and both hypophysectomized and control rats were fasted 15 hours before sacrifice.

The Transaminase Content of Parenchymatous Liver Cells

1959 ◽  
Vol 36 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Keith S. Henley ◽  
Hugh S. Wiggins ◽  
H. Marvin Pollard ◽  
Esther Dullaert
Keyword(s):  
Liver Cells

Nitric Oxide Protects Murine Embryonic Liver Cells (BNL CL.2) from Cytotoxicity Induced by Glucose Deprivation

2000 ◽  
Vol 86 (3) ◽  
pp. 140-144 ◽  
Author(s):  
Hyun-Ock Pae ◽  
Hong-Gone Kim ◽  
Young-Suk Paik ◽  
Sang-Gi Paik ◽  
Young-Myeong Kim ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Glucose Deprivation ◽  
Liver Cells ◽  
Embryonic Liver
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